Abstract
Two new species of Impatiens (Balsaminaceae) from Yunnan, China, are here described. Impatiens rostrisepala can be easily recognized by its pale purplish-red (rarely white) flowers, and lower sepal being deeply infundibuliform, concave near the mouth on the lower side, and usually with a long beak at the mouth. Impatiens leptophylla differs from its closely related species in having lamina with an acute apex, venation with prominent midrib and indistinct tertiary veins adaxially, yellow flowers, and lower sepal spur with a non-circinnate apex. Molecular phylogenetic analyses based on both nuclear ribosomal and plastid DNA sequences further support the recognition of these two taxa as distinct species.
References
- Akiyama, S., Ohba, H., Sugawara, T., Yang, Y.P. & Murata, J. (1995) Notes of Impatiens (Balsaminaceae) from Southwestern Yunnan. Journal of Japanese Botany 70: 95–106.
- Capella-Gutiérrez, S., Silla-Martínez, J.M. & Gabaldón, T. (2009) trimAl: a tool for automated alignment trimming in large-scale phylogenetic analyses. Bioinformatics 25 (15): 1972–1973. https://doi.org/10.1093/bioinformatics/btp348
- Chen, L., Dong, H.J. & Peng, H. (2013) Diversity and distribution of higher plants in Yunnan, China. Biodiversity Science 21: 359–363. https://doi.org/10.3724/SP.J.1003.2013.05162
- Chen, Y.L. (2001) Balsaminaceae. In: Delectis Florae Reipublicae Popularis Sinicae Agenda Academiae Sinicae Edita (Org.) Flora Reipublicae Popularis Sinica, Vol. 47 (2). Science Press, Beijing, pp. 1–231.
- Chen, Y.L., Akiyama, S. & Ohba, H. (2007) Balsaminaceae. In: Wu, Z.Y., Raven, P.H. & Hong, D.Y. (Eds.) Flora of China, Vol. 12. Science Press, Beijing & Missouri Botanical Garden Press, St. Louis, pp. 43–114.
- Comber, H.F. (1934) Diagnoses specierum novarum in herbario Horti Regii Botanici Edinburgensis cognitarum DCI-DCXIII. Notes from the Royal Botanic Garden Edinburgh 18 (89): 221–249. https://doi.org/10.24823/nrbge.1934.2390
- Cong, Y.Y., Hu, G.W., Liu, K.M. & Wang, Q.F. (2025) Plants of Balsaminaceae in China. Hubei Science & Technology Press, Wuhan, 313 pp.
- Doyle, J.J. & Doyle, J.L. (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin 19 (1): 11–15.
- Grey-Wilson, C. (1980) Impatiens of Africa. Balkema, Rotterdam, 235 pp.
- Handel-Mazzetti, H. (1933) Symbolae Sinicae: Botanische Ergebnisse der Expedition der Akademie der Wissenschaften in Wien nach Südwest-China 1914–1918, Vol. 7 (3). Julius Springer, Wien, 730 pp.
- Hooker, J.D. (1908) Les espèces du genre Impatiens dans l’herbier du muséum de Paris. Nouvelles Archives du Muséum d’Histoire Naturelle sér. 4 10: 233–272.
- Hooker, J.D. & Thomsom, T. (1859) Præcursores ad floram Indicam: Balsamineæ. Botanical Journal of the Linnean Society 4: 106–157. https://doi.org/10.1111/j.1095-8339.1859.tb01160.x
- Hu, T., Peng, S., Zhou, X.X., Zheng, Y.M., Cong, Y.Y. & Hu, G.W. (2022) Two new species of Impatiens (Balsaminaceae) from Gaoligong Mountains, Yunnan, China. Phytotaxa 566 (3): 268–278. https://doi.org/10.11646/phytotaxa.566.3.2
- Jin, J.J., Yu, W.B., Yang, J.B., Song, Y., DePamphilis, C.W., Yi, T.S. & Li, D.Z. (2020) GetOrganelle: a fast and versatile toolkit for accurate de novo assembly of organelle genomes. Genome Biology 21 (1): 241. https://doi.org/10.1186/s13059-020-02154-5
- Kalyaanamoorthy, S., Minh, B.Q., Wong, T.K.F., von Haeseler, A. & Jermiin, L.S. (2017) ModelFinder: fast model selection for accurate phylogenetic estimates. Nature Methods 14: 587–589. https://doi.org/10.1038/nmeth.4285
- Katoh, K. & Standley, D.M. (2013) MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Molecular Biology and Evolution 30 (4): 772–780. https://doi.org/10.1093/molbev/mst010
- Lanfear, R., Frandsen, P.B., Wright, A.M., Senfeld, T. & Calcott, B. (2017) PartitionFinder 2: new methods for selecting partitioned models of evolution for molecular and morphological phylogenetic analyses. Molecular Biology and Evolution 34 (3): 772–773. https://doi.org/10.1093/molbev/msw260
- Liang, Y.F., Xue, T.T., Gadagkar, S.R., Qin, F., Janssens, S.B. & Yu, S.X. (2025) Phylogenomic conflict analyses of plastid and mitochondrial genomes of Impatiens (Balsaminaceae) reveal its complex evolutionary history. Molecular Phylogenetics and Evolution 206: 108325. https://doi.org/10.1016/j.ympev.2025.108325
- Liao, R.L., Cai, L., Yu, Z.Y., Wang, Y.H. & Sun, W.B. (2021) Impatiens wutaishanensis (Balsaminaceae), a new species from Southeast Yunnan, China. PhytoKeys 176: 43–53. https://doi.org/10.3897/phytokeys.176.58825
- Linnaeus, C. (1753) Species plantarum 2. Salvius, Stockholm, pp. 561–1200.
- Minh, B.Q., Nguyen, M.A. & von Haeseler, A. (2013) Ultrafast approximation for phylogenetic bootstrap. Molecular Biology and Evolution 30 (5): 1188–1195. https://doi.org/10.1093/molbev/mst024
- Nguyen, L.T., Schmidt, H.A., von Haeseler, A. & Minh, B.Q. (2015) IQ-TREE: a fast and effective stochastic algorithm for estimating maximum-likelihood phylogenies. Molecular Biology and Evolution 32 (1): 268–274. https://doi.org/10.1093/molbev/msu300
- Peng, S., Rono, P.C., Yang, J.X., Wang, J.J., Hu, G.W. & Wang, Q.F. (2021) Description of a new species and lectotypification of two names in Impatiens sect. Racemosae (Balsaminaceae) from China. Plants 10: 1812. https://doi.org/10.3390/plants10091812
- Qin, F., Xue, T., Zhang, X., Yang, X., Yu, J., Gadagkar, S.R. & Yu, S.X. (2023) Past climate cooling and orogenesis of the Hengduan Mountains have influenced the evolution of Impatiens sect. Impatiens (Balsaminaceae) in the Northern Hemisphere. BMC Plant Biology 23 (1): 600. https://doi.org/10.1186/s12870-023-04625-w
- Ronquist, F., Teslenko, M., van der Mark, P., Ayres, D.L., Darling, A., Höhna, S., Larget, B., Liu, L., Suchard, M.A. & Huelsenbeck, J.P. (2012) MrBayes 3.2: Efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology 61 (3): 539–542. https://doi.org/10.1093/sysbio/sys029
- Shimizu, T. & Suksathan, P. (2004) Three new species of the Thai Impatiens (Balsaminaceae). part 3. Bulletin of the National Science Museum Series B (Botany) 30 (4): 165–171.
- Stöver, B.C. & Müller, K.F. (2010) TreeGraph 2: Combining and visualizing evidence from different phylogenetic analyses. BMC Bioinformatics 11: 7. https://doi.org/10.1186/1471-2105-11-7
- Wang, Z.W., Wang, Q., Xu, R.H., Zhang, Y. & Li, X.C. (2022) Impatiens chenmoui (Balsaminaceae), a new species from southern Yunnan, China. PhytoKeys 214: 83–95. https://doi.org/10.3897/phytokeys.214.94898
- Wight, R. & Walker-Arnott, G.A. (1834) Prodromus Florae Peninsulae Indiae Orientalis: containing abridged descriptions of the plants found in the peninsula of British India, arranged according to the natural system 1. Parbury, Allen & Co., London, 480 pp. https://doi.org/10.5962/bhl.title.252
- Xue, T., Yu, J., Gadagkar, S.R., Qin, F., Zhang, X., An, M.T. & Yu, S.X. (2025) Phylogeny and biogeography of Impatiens sect. Racemosae (Balsaminaceae) based on nrDNA and plastome sequences, emphasizing diversification in the Himalaya and the Hengduan Mountains. Taxon 74 (4): 874–889. https://doi.org/10.1002/tax.13352
- Yu, J.H., Zhang, W.D., Qin, F., Xia, C.Y., Qin, Y., An, M.T. & Yu, S.X. (2022) Impatiens yunlingensis (Balsaminaceae), a new species from Yunnan, China. PhytoKeys 212: 13–27. https://doi.org/10.3897/phytokeys.212.89347
- Yu, S.X., Janssens, S.B., Zhu, X.Y., Lidén, M., Gao, T.G. & Wang, W. (2016) Phylogeny of Impatiens (Balsaminaceae): Integrating molecular and morphological evidence into a new classification. Cladistics 32 (2): 179–197. https://doi.org/10.1111/cla.12119
- Yuan, T.H., Li, M.J., Ren, L.Y., Huang, R.X., Chen, Y. & Bai, X.X. (2022) A dataset on the diversity and geographical distributions of wild Impatiens in China. Biodiversity Science 30: 22019. https://doi.org/10.17520/biods.2022019
- Zeng, Y.M., Zhang, G.L., Wu, N.F., Hu, T. & Cong, Y.Y. (2023) Impatiens spiralis (Balsaminaceae), a new species from Yunnan, China. Nordic Journal of Botany 2023 (8): e03862. https://doi.org/10.1111/njb.03862
- Zhang, D., Gao, F., Jakovlić, I., Zou, H., Zhang, J., Li, W.X. & Wang, G.T. (2020) PhyloSuite: An integrated and scalable desktop platform for streamlined molecular sequence data management and evolutionary phylogenetics studies. Molecular Ecology Resources 20 (1): 348–355. https://doi.org/10.1111/1755-0998.13096
