Skip to main content Skip to main navigation menu Skip to site footer
Responsive image
Issue: Vol. 774 No. 2: 15 Sept. 2026
Type: Article
Published: 2026-09-15
DOI: 10.11646/phytotaxa.774.2.6
Page range: 173-182
Abstract views: 29
PDF downloaded: 0

Nigropunctata xishuangbannaensis sp. nov. (Pallidoperidiaceae, Xylariales), a new species from bamboo in Yunnan, China

State Key Laboratory of Discovery and Utilization of Functional Components in Traditional Chinese Medicine & School of Pharmaceutical Sciences; Guizhou Medical University; Guian New District; Guizhou 561113; P.R. China; The High Efficacy Application of Natural Medicinal Resources Engineering Center of Guizhou Province (The Key Laboratory of Optimal Utilization of Natural Medicine Resources); School of Pharmaceutical Sciences; Guizhou Medical University; Guian New District; Guizhou 561113; P.R. China
State Key Laboratory of Discovery and Utilization of Functional Components in Traditional Chinese Medicine & School of Pharmaceutical Sciences; Guizhou Medical University; Guian New District; Guizhou 561113; P.R. China; The High Efficacy Application of Natural Medicinal Resources Engineering Center of Guizhou Province (The Key Laboratory of Optimal Utilization of Natural Medicine Resources); School of Pharmaceutical Sciences; Guizhou Medical University; Guian New District; Guizhou 561113; P.R. China
State Key Laboratory of Discovery and Utilization of Functional Components in Traditional Chinese Medicine & School of Pharmaceutical Sciences; Guizhou Medical University; Guian New District; Guizhou 561113; P.R. China; The High Efficacy Application of Natural Medicinal Resources Engineering Center of Guizhou Province (The Key Laboratory of Optimal Utilization of Natural Medicine Resources); School of Pharmaceutical Sciences; Guizhou Medical University; Guian New District; Guizhou 561113; P.R. China
State Key Laboratory of Discovery and Utilization of Functional Components in Traditional Chinese Medicine & School of Pharmaceutical Sciences; Guizhou Medical University; Guian New District; Guizhou 561113; P.R. China; The High Efficacy Application of Natural Medicinal Resources Engineering Center of Guizhou Province (The Key Laboratory of Optimal Utilization of Natural Medicine Resources); School of Pharmaceutical Sciences; Guizhou Medical University; Guian New District; Guizhou 561113; P.R. China
State Key Laboratory of Discovery and Utilization of Functional Components in Traditional Chinese Medicine & School of Pharmaceutical Sciences; Guizhou Medical University; Guian New District; Guizhou 561113; P.R. China; The High Efficacy Application of Natural Medicinal Resources Engineering Center of Guizhou Province (The Key Laboratory of Optimal Utilization of Natural Medicine Resources); School of Pharmaceutical Sciences; Guizhou Medical University; Guian New District; Guizhou 561113; P.R. China
Fungal Biology and Systematics Research Laboratory; Institute of Botany; University of the Punjab; Quaid-e-Azam Campus 54590; Lahore; Pakistan
Centre of Advanced Study in Botany; Institute of Science; Banaras Hindu University; Varanasi 221005; Uttar Pradesh; India
State Key Laboratory of Discovery and Utilization of Functional Components in Traditional Chinese Medicine & School of Pharmaceutical Sciences; Guizhou Medical University; Guian New District; Guizhou 561113; P.R. China; The High Efficacy Application of Natural Medicinal Resources Engineering Center of Guizhou Province (The Key Laboratory of Optimal Utilization of Natural Medicine Resources); School of Pharmaceutical Sciences; Guizhou Medical University; Guian New District; Guizhou 561113; P.R. China; Guizhou Key Laboratory of Microbio and Infectious Disease Prevention & Control; Guizhou Medical University; Guian New District; Guizhou 561113; P.R. China
Fungi bambusicolous fungi microfungi molecular systematics Xylariales

Abstract

Nigropunctata xishuangbannaensis sp. nov. is introduced in this study, isolated from decaying bamboo culms in Yunnan Province, China. Multigene phylogenetic analyses based on five loci (ITS, LSU, rpb2, tub2, and tef1-α) place this taxon in a well-supported, distinct lineage clearly separated from closely related species in the genus, supporting its status as a novel species. Morphologically, the new species differs from its phylogenetically close relatives, N. chiangraiensis and N. conspicua, by the absence of a mucilaginous sheath and its ellipsoidal ascospores with pointed ends. A detailed comparative morphological analysis of N. xishuangbannaensis and related or similar species is also provided.

References

  1. Boonmee, S., Wanasinghe, D.N., Calabon, M.S., Huanraluek, N., Chandrasiri, S.K.U., Jones, G.E.B., Rossi, W., Leonardi, M., Singh, S.K., Rana, S., Singh, P.N., Maurya, D.K., Lagashetti, A.C., Choudhary, D., Dai, Y.C., Zhao, C.L., Mu, Y.H., Yuan, H.S., He, S.H., Phookamsak, R., Jiang, H.B., Martín, M.P., Dueñas, M., Telleria, M.T., Kałucka, I.L., Jagodziński, A.M., Liimatainen, K., Pereira, D.S., Phillips, A.J.L., Suwannarach, N., Kumla, J., Khuna, S., Lumyong, S., Potter, T.B., Shivas, R.G., Sparks, A.H., Vaghefi, N., Abdel-Wahab, M.A., AbdelAziz, F.A., Li, G.J., Lin, W.F., Singh, U., Bhatt, R.P., Lee, H.B., Nguyen, T.T.T., Kirk, P.M., Dutta, A.K., Acharya, K., Sarma, V.V., Niranjan, M., Rajeshkumar, K.C., Ashtekar, N., Lad, S., Wijayawardene, N.N., Bhat, D.J., Xu, R.J., Wijesinghe, S.N., Shen, H.W., Luo, Z.L., Zhang, J.Y., Sysouphanthong, P., Thongklang, N., Bao, D.F., Aluthmuhandiram, J.V.S., Abdollahzadeh, J., Javadi, A., Dovana, F., Usman, M., Khalid, A.N., Dissanayake, A.J., Telagathoti, A., Probst, M., Peintner, U., Garrido-Benavent, I., Bóna, L., Merényi, Z., Boros, L., Zoltán, B., Stielow, J.B., Jiang, N., Tian, C.M., Shams, E., Dehghanizadeh, F., Pordel, A., Javan-Nikkhah, M., Denchev, T.T., Denchev, C.M., Kemler, M., Begerow, D., Deng, C.Y., Harrower, E., Bozorov, T., Kholmuradova, T., Gafforov, Y., Abdurazakov, A., Xu, J.C., Mortimer, P.E., Ren, G.C., Jeewon, R., Maharachchikumbura, S.S.N., Phukhamsakda, C., Mapook, A. & Hyde, K.D. (2021) Fungal diversity notes 1387–1511: Taxonomic and phylogenetic contributions on genera and species of fungal taxa. Fungal Diversity 111: 1–335. https://doi.org/10.1007/s13225-021-00489-3
  2. Cao, B., Phurbu, D., Ralaiveloarisoa, A., Liimatainen, K., Niskanen, T., Ramírez-Cruz, V., Bradshaw, A.J., Dentinger, B.T.M., Ramírez-Guillén, F., Cortés-Pérez, A., Guzmán-Dávalos, L., Villalobos-Arámbula, A.R., Castro-Jauregui, O., Silva, P.S., Day, R., Davoodian, N., Lebel, T., Castellano, M., May, T.W., Toome, M., Vasey, J., Hofer, K., Thangavel, R., Braithwaite, M., Braithwaite, L., Dutta, A.K., Chattopadhyay, P., Roy, N., Tanti, B., Biswas, P.R., Arumugam, E., Kezo, K., Kaliyaperumal, M., Murugadoss, R., Ji, J.X., Kakishima, M., Cooper, J., Nuytinck, J., Luo, Z.L., Wang, W.P., Zhang, X., Wen, T.C., Jia, A.L., Fan, X.L., Jiang, N., Jiao, N., Zhang, Y., Manawasinghe, I.S., Li, H., Xiong, Y.R., Zhou, T., Tan, Q.L., Hoshino, T., Lagaet, P., De Lange, R., Verbeken, A., Tondeleir, L., Manz, C., Hampe, F., Zhao, C.L., Yang, Y., Zhang, G.Q., Dai, D.Q., Tomšovský, M., Denchev, T.T., Denchev, C.M., Léveillé-Bourret, É., Kemler, M., Chen, Y.P., Maharachchikumbura, S.S.N., Feng, Z.X., Wang, L., Wang, Q.M., Wan, S.P., Yu, F.Q., Liu, J.K., Du, H.Z., Wu, N., Hu, H.L., Su, J.Y., Wang, Z.H., Hu, Y.P., Yu, H., Wang, J., Yang, Y.H., Dong, W., Shu, Y.X., Zhao, H.J., Wang, C.Q., Xia, W.X., Liao, C.F., Doilom, M., Li, G.J., Suwannarach, N., Senwanna, C., Gafforov, Y., Flakus, A., Suchan, T., Plata, O., Rodriguez-Flakus, P., Tamang, J., Acharya, K., Yang, H.D., Jayawardena, R.S., Tiendrebeogo, A., Decock, C., Somda, I., Legrève, A., Yang, Y., Xiao, Y.P., Zhou, X.Z., Li, J.X., Thongklang, N., Han, X.X., Yang, W.Q., Wang, S.H., He, M.Q., Hyde, K.D. & Zhao, R.L. (2025) Fungal diversity notes 1919–2016: Taxonomic and phylogenetic contributions to fungal taxa. Fungal Diversity 132: 425–651. https://doi.org/10.1007/s13225-025-00556-z
  3. Corbu, V.M., Gheorghe-Barbu, I., Dumbravă, A.Ș., Vrâncianu, C.O. & Șesan, T.E. (2023) Current Insights in Fungal Importance—A Comprehensive Review. Microorganisms 11 (6): 1384. https://doi.org/10.3390/microorganisms11061384
  4. Gardes, M. & Bruns, T.D. (1993) ITS primers with enhanced specificity for basidiomycetes—Application to the identification of mycorrhizae and rusts. Molecular Ecology 2 (2): 113–118. https://doi.org/10.1111/j.1365-294X.1993.tb00005.x
  5. Glass, N.L. & Donaldson, G.C. (1995) Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. Applied and Environmental Microbiology 61 (4): 1323–1330. https://doi.org/10.1128/aem.61.4.1323-1330.1995
  6. Habib, K., Li, W.H., Ren, Y.L., Liu, L.L., Lu, C.T., Zhang, Q.F., Yao, Z.Q., Luo, X.Y., Zhou, X., Zeng, W.Y., Kang, Y.Q., Shen, X.C., Wijayawardene, N.N., Elgorban, A.M., Al-Rejaie, S. & Li, Q.R. (2025) Exploration of ascomycetous Fungi revealing novel taxa in Southwestern China. Mycosphere 16 (1): 1412–1529. https://doi.org/10.5943/mycosphere/16/1/8
  7. Hall, T.A. (1999) BioEdit: A user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 41: 95–98.
  8. Huelsenbeck, J.P., Ronquist, F., Nielsen, R. & Bollback, J.P. (2001) Bayesian inference of phylogeny and its impact on evolutionary biology. Science 294: 2310–2314. https://doi.org/10.1126/science.1065889
  9. Hyde, K.D., Norphanphoun, C., Chen, J., Dissanayake, A.J., Doilom, M., Hongsanan, S., Jayawardena, R.S., Jeewon, R., Perera, R.H., Thongbai, B. & Wanasinghe, D.N. (2018) Thailand’s amazing diversity: up to 96% of fungi in northern Thailand may be novel. Fungal Diversity 93: 215–239. https://doi.org/10.1007/s13225-018-0415-7
  10. Katoh, K., Rozewicki, J. & Yamada, K.D. (2019) MAFFT online service: Multiple sequence alignment, interactive sequence choice and visualization. Briefings in Bioinformatics 20 (4): 1160–1166. https://doi.org/10.1093/bib/bbx108
  11. Li, Q.R., Habib, K., Long, S.H., Wu, Y.P., Zhang, X., Hu, H.M., Wu, Q.Z., Liu, L.L., Zhou, S.X., Shen, X.C. & Kang, J.C. (2024) Unveiling fungal diversity in China: New species and records within the Xylariaceae Family. Mycosphere 15 (1): 275–364. https://doi.org/10.5943/mycosphere/15/1/2
  12. Li, W.H., Habib, K., Yao, Z.Q., Wang, G.Y., Wang, X.Y., Li, Q.R. & Kang, J.C. (2026) First Complete Mitochondrial Genome of Nigropunctata complanata—A New Record for China. Phytotaxa 752 (2): 150–162. https://doi.org/10.11646/phytotaxa.752.2.5
  13. Liu, L.L., Ren, Y.L., Habib, K., Lu, C.T., Wu, Y.P., Long, S.H., Lin, Y., Zhang, X., Kang, Y.Q., Wijayawardene, N.N., Wang, F., Elgorban, A.M., Al-Rejaie, S., Samarakoon, M.C., Shen, X.C. & Li, Q.R. (2025) New taxa of Xylariales from Karst Ecosystems in Southwestern China. Mycosphere 16 (1): 1–78. https://doi.org/10.5943/mycosphere/16/1/1
  14. Liu, Y.J., Whelen, S. & Hall, B.D. (1999) Phylogenetic relationships among ascomycetes: Evidence from an RNA polymerase II subunit. Molecular Biology and Evolution 16: 1799–1808. https://doi.org/10.1093/oxfordjournals.molbev.a026092
  15. Lu, C.T., Ren, Y.L., Habib, K., Zhang, Q.F., Liu, L.L., Kang, J.C., Shen, X.C., Wijayawardene, N.N., Al Rejaie, S.S., Loinheuang, C., Li, Q.R., Long, Q.D. & Elgorban, A.M. (2025) Two new species of Nigropunctata and the first report of sexual morph of Melanographium citri (Pallidoperidiaceae, Xylariales) from south-western China. MycoKeys 122: 257–275. https://doi.org/10.3897/mycokeys.122.161215
  16. Miller, M., Pfeiffer, W.T. & Schwartz, T. (2010) Creating the CIPRES science gateway for inference of large phylogenetic trees. Proceedings of the Gateway Computing Environments Workshop 14: 1–8. https://doi.org/10.1109/GCE.2010.5676129
  17. Rambaut, A. (2018) FigTree v1.4.4: Tree figure drawing tool. Available from: http://tree.bio.ed.ac.uk/software/figtree (accessed 29 Dec 2025)
  18. Rehner, S.A. & Buckley, E. (2005) A Beauveria phylogeny inferred from nuclear ITS and EF1-alpha sequences: Evidence for cryptic diversification and links to Cordyceps teleomorphs. Mycologia 97 (1): 84–98. https://doi.org/10.3852/mycologia.97.1.84
  19. Ren, G.C., Jayasiri, S.C., Tibpromma, S., De Farias, A.R.G., Chethana, K.W.T., Faraj, K.H., Wanasinghe, D.N., Xu, J.C., Hyde, K.D. & Gui, H. (2024) Saprobic ascomycetes associated with woody litter from the Greater Mekong Subregion (Southwestern China and Northern Thailand). Mycosphere 15 (1): 954–1082. https://doi.org/10.5943/mycosphere/15/1/8
  20. Ronquist, F., Teslenko, M., van der Mark, P., Ayres, D.L., Darling, A., Höhna, S., Larget, B., Liu, L., Suchard, M.A. & Huelsenbeck, J.P. (2012) MrBayes 3.2: Efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology 61 (3): 539–542. https://doi.org/10.1093/sysbio/sys029
  21. Samarakoon, M.C., Hyde, K.D., Maharachchikumbura, S.S.N., Stadler, M., Gareth Jones, E.B., Promputtha, I., Suwannarach, N., Camporesi, E., Bulgakov, T.S. & Liu, J.-K. (2022) Taxonomy, phylogeny, molecular dating and ancestral state reconstruction of Xylariomycetidae (Sordariomycetes). Fungal Diversity 112 (1): 1–88. https://doi.org/10.1007/s13225-021-00495-5
  22. Samarakoon, M.C., Lumyong, S., Manawasinghe, I.S., Suwannarach, N. & Cheewangkoon, R. (2023) Addition of Five Novel Fungal Flora to the Xylariomycetidae (Sordariomycetes, Ascomycota) in Northern Thailand. Journal of Fungi (Basel, Switzerland) 9 (11): 1065. https://doi.org/10.3390/jof9111065
  23. Stamatakis, A. (2014) RAxML version 8: A tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics (Oxford, England) 30 (9): 1312–1313. https://doi.org/10.1093/bioinformatics/btu033
  24. Sugita, R., Yoshioka, R. & Tanaka, K. (2024) Anthostomella-like fungi on bamboo: Four new genera belonging to a new family Pallidoperidiaceae (Xylariales). Mycoscience 65 (1): 28–46. https://doi.org/10.47371/mycosci.2023.11.005
  25. Tun, Z.L., Gomdola, D., Bhunjun, C.S., Maharachchikumbura, S.S.N., Alotibi, F. & Hyde, K.D. (2024) A novel species and a new geographical record of Nigropunctata from Bambusa vulgaris in northern Thailand. Phytotaxa 676 (2): 155–168. https://doi.org/10.11646/phytotaxa.676.2.4
  26. Vilgalys, R. & Hester, M. (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. Journal of Bacteriology 172 (8): 4238–4246. https://doi.org/10.1128/jb.172.8.4238-4246.1990
  27. White, T.J., Bruns, T., Lee, S.J.W.T. & Taylor, J. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR protocols: a guide to methods and applications, 18 (1). pp. 315–322. https://doi.org/10.1016/B978-0-12-372180-8.50042-1
  28. Zhang, S.N., Hyde, K.D., Jones, E.G., Yu, X.D., Cheewangkoon, R. & Liu, J.K. (2024) Current insights into palm fungi with emphasis on taxonomy and phylogeny. Fungal Diversity 127 (1): 55–301. https://doi.org/10.1007/s13225-024-00536-9
  29. Zhou, X., Habib, K., Zeng, W., Ren, Y., Shen, X., Kang, J. & Li, Q. (2024) Addition of three new species of Xylariomycetidae fungi on bamboo from Southern China. MycoKeys 109: 109–129. https://doi.org/10.3897/mycokeys.109.128020

How to Cite

Zheng, S.-L., Liu, N.-F., Khurshid, R., Habib, K., Solomon, B., Afshan, N.-U.-S., Singh, R. & Li, Q.-R. (2026) Nigropunctata xishuangbannaensis sp. nov. (Pallidoperidiaceae, Xylariales), a new species from bamboo in Yunnan, China. Phytotaxa 774 (2): 173–182. https://doi.org/10.11646/phytotaxa.774.2.6