Skip to main content Skip to main navigation menu Skip to site footer
Responsive image
Issue: Vol. 774 No. 1: 11 Sept. 2026
Type: Article
Published: 2026-09-11
DOI: 10.11646/phytotaxa.774.1.4
Page range: 49-59
Abstract views: 24
PDF downloaded: 1

Coryneum chengduense sp. nov. (Coryneaceae, Diaporthales) from Sichuan Province, China

School of Life Science and Technology; Center for Informational Biology; University of Electronic Science and Technology of China; Chengdu 611731; P.R. China
School of Life Science and Technology; Center for Informational Biology; University of Electronic Science and Technology of China; Chengdu 611731; P.R. China
Pests and Plant Diseases Unit; College of Agricultural and Food Sciences; King Faisal University; Al-Ahsa-31982; Saudi Arabia
School of Life Science and Technology; Center for Informational Biology; University of Electronic Science and Technology of China; Chengdu 611731; P.R. China
Fungi asexual morph fungal diversity lignicolous fungi Sordariomycetes

Abstract

During our ongoing survey of fungal diversity in Sichuan Province, China, we isolated an unidentified fungus collected from a dead branch of an unidentified woody angiosperm. Based on the initial BLAST results of the internal transcribed spacer (ITS), the isolate was preliminarily identified as a species of Coryneum. To clarify its taxonomic status, we used the combined ITS, the partial nuclear ribosomal large subunit rRNA (LSU), the RNA polymerase II second largest subunit region (rpb2), and the translation elongation factor 1-α region (tef1-α) for phylogenetic analyses, which showed that the isolate forms a distinct lineage within Coryneum. Morphologically, Coryneum chengduense can be distinguished from its phylogenetically close species, C. lanciforme and C. depressum, by its smaller conidia and fewer distosepta. Therefore, based on morphological and phylogenetic evidence, we introduce this collection as Coryneum chengduense sp. nov.

References

  1. Capella-Gutiérrez, S., Silla-Martínez, J.M. & Gabaldón, T. (2009) trimAl: A Tool for automate alignment trimming in large-scale phylogenetic analyses. Bioinformatics 25 (15): 1972–1973. https://doi.org/10.1093/bioinformatics/btp348
  2. Cesati, V. & De Notaris, G. (1863) Schema di classificazione degle sferiacei italici aschigeri piu’ o meno appartenenti al genere Sphaeria nell’antico significato attribuitoglide Persono. Commentario della Società Crittogamologica Italiana 1: 177–420.
  3. Chen, C.J., Wu, Y., Li, J.W., Wang, X., Zeng, Z.H., Xu, J., Liu, Y.L., Feng, J.T., Chen, H., He, Y.H. & Xia, R. (2023) TBtools-II: A “one for all, all for one” bioinformatics platform for biological big-data mining. Molecular Plant 16: 1733–1742. https://doi.org/10.1016/j.molp.2023.09.010
  4. Chen, Y.P., Su, P.W., Stadler, M., Xiang, R., Hyde, K.D., Tian, W.H. & Maharachchikumbura, S.S.N. (2023) Beyond observation: genomic traits and machine learning algorithms for predicting fungal lifestyles. Mycosphere 14 (1): 1530–1563. https://doi.org/10.5943/mycosphere/14/1/17
  5. Fan, X.L., Du, Z., Bezerra, J.D.P. & Tian, C.M. (2018) Taxonomic circumscription of melanconis-like fungi causing canker disease in China. MycoKeys 42: 89–124. https://doi.org/10.3897/mycokeys.42.29634
  6. Glass, N.L. & Donaldson, G.C. (1995) Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous Ascomycetes. Applied and Environmental Microbiology 61 (4): 1323–1330. https://doi.org/10.1128/aem.61.4.1323-1330.1995
  7. Glez-Pen, D., Gomez-Blanco, D., Reboiro-Jato, M., Fdez-Riverola, F. & Posada, D.A. (2010) ALTER: program-oriented conversion of DNA and protein alignments. Nucleic Acids Research 38 (suppl_2): W14–W18. https://doi.org/10.1093/nar/gkq321
  8. Hall, T.A. (1999) BioEdit: A User-Friendly Biological Sequence Alignment Editor and Analysis Program for Windows 95/98/NT. Nucleic Acids Symposium Series 41: 95–98.
  9. Hibbett, D.S. (1996) Phylogenetic evidence for horizontal transmission of group I introns in the nuclear ribosomal DNA of mushroom-forming fungi. Molecular Biology and Evolution 13 (7): 903–917. https://doi.org/10.1093/oxfordjournals.molbev.a025658
  10. Hongsanan, S., Khuna, S., Manawasinghe, I.S., Tibpromma, S., Chethana, K.W.T., Xie, N., Bagacay, J.F.E., Calabon, M.S., Chen, C., Doilom, M., Du, H.Y., Gafforov, Y., Huang, S.K., Li, J.X., Luangharn, T., Luo, Z.L., Opiña, L.A.D., Pem, D., Sadaba, R.B., Singh, R., Tan, Q., Tang, S.M., Wang, W.P., Wen, T.C., Xia, G., Zhao, Q., Bhunjun, C.S., Cao, B., Chen, Y.P., de Silva, N.I., Dai, D.Q., Dong, W., Du, T.Y., Ferreira-Sá, A.S., Gao, Y., Gui, H., Han, L.S., Han, M.Y., Han, X.X., Jayawardena, R.S., Khyaju, S., Kumar, S., Lei, L., Leonardo-Silva, L., Li, H., Li, Y.X., Liao, C.F., Liu, J.W., Liu, X.F., Lu, L., Lu, W.H., Luo, M., Maharachchikumbura, S.S.N., Meng, Q.F., Mi, L.X., Norphanphoun, C., Peng, X.C., Su, H.L., Tennakoon, D.S., Thiyagaraja, V., Tun, Z.L., Wijayawardene, N.N., Xavier-Santos, S., Xiong, Y.R., Xu, R.F., Yadav, S., Yang, T., Yang, Y.H., Yarasheva, M., Zeng, X.Y., Zhang, H., Zhang, G.Q., Zhang, X., Zhao, H.J., Zhao, R.L., Zheng, D., Wanasinghe, D.N. & Karunarathna, S.C. (2025) Mycosphere Notes 521–571: A special edition of fungal biodiversity to celebrate Kevin D. Hyde's 70th birthday and his exceptional contributions to Mycology. Mycosphere 16 (2): 2002–2180. https://doi.org/10.5943/mycosphere/16/2/1
  11. Hyde, K.D., Norphanphoun, C., Maharachchikumbura, S.S.N., Bhat, D.J., Jones, E.B.G., Bundhun, D., Chen, Y.J., Bao, D.F., Boonmee, S., Calophyllu, M.S., Chaiwan, N., Chethana, K.W.T., Dai, D.Q., Dayarathne, M.C., Devadatha, B., Dissanayake, A.J., Dissanayake, L.S., Doilom, M., Dong, W., Fan, X.L., Goonasekara, I.D., Hongsanan, S., Huang, S.K., Jayawardena, R.S., Jeewon, R., Karunarathna, A., Konta, S., Kumar, V., Lin, C.G., Liu, J.K., Liu, N.G., Luangsa-ard, J., Lumyong, S., Luo, Z.L., Marasinghe, D.S., McKenzie, E.H.C., Niego, A.G.T., Niranjan, M., Perera, R.H., Phukhamsakda, C., Rathnayaka, A.R., Samarakoon, M.C., Samarakoon, S.M.B.C., Sarma, V.V., Senanayake, I.C., Shang, Q.J., Stadler, M., Tibpromma, S., Wanasinghe, D.N., Wei, D.P., Wijayawardene, N.N., Xiao, Y.P., Yang, J., Zeng, X.Y., Zhang, S.N. & Xiang, M.M. (2020) Refined families of Sordariomycetes. Mycosphere 11 (1): 305–1059. https://doi.org/10.5943/mycosphere/11/1/7
  12. Jia, A., Chen, B., Lu, H., Xing, Y., Li, B. & Fan, X. (2024) Multigene phylogeny and morphology reveal three new species of Cytospora isolated from diseased plant branches in Fengtai District, Beijing, China. MycoKeys 101: 163–189. https://doi.org/10.3897/mycokeys.101.116272
  13. Jiang, N., Voglmayr, H. & Tian, C.M. (2018) New species and records of Coryneum from China. Mycologia 110: 1172–1188. https://doi.org/10.1080/00275514.2018.1516969
  14. Jiang, N., Voglmayr, H. & Tian, C.M. (2019) Morphology and phylogeny reveal two novel Coryneum species from China. MycoKeys 10 (56): 67–80. https://doi.org/10.3897/mycokeys.56.35554
  15. Larsson, A. (2014) AliView: A fast and lightweight alignment viewer and editor for large datasets. Bioinformatics 30 (22): 3276–3278. https://doi.org/10.1093/bioinformatics/btu531
  16. Long, C.S., Wu, Y.P., Zhang, X., Lin, Y., Shen, X.C., Ma, J. & Li, Q.R. (2023) Additions to hyphomycetes from Yungui Plateau, China with three new species (Ascomycota, Sordariomycetes). Biodiversity Data Journal 11: e101629. https://doi.org/10.3897/BDJ.11.e101629
  17. Madhushan, A., Weerasingha, D.B., Ilyukhin, E., Taylor, P.W.J., Ratnayake, A.S., Liu, J.K. & Maharachchikumbura, S.S.N. (2024) An overview of symbiotic and pathogenic interactions at the fungi-plant interface under environmental constraints. Frontiers in Fungal Biology 5: 1363460. https://doi.org/10.3389/ffunb.2024.1363460
  18. Maharachchikumbura, S.S.N., Mahadevakumar, S., Hyde, K.D., Al-Sadi, A.M., Chalasani, D., Chandranayaka, S., Dissanayake, L.S., Faraj, T.K.H., Fernando, W.G.D., Gunasinghe, N., Ilyukhin, E., Kumar, S., Lateef, A.A., Madhushan, A., McKenzie, E.H.C., Pandey, A.K., Podile, A.R., Prasannath, K., Sarma, P.V.S.R.N., Taylor, P.W.J. & Wanasinghe, D.N. (2026) The 50 most researched fungal and oomycete plant pathogens. Fungal Diversity 136: 136004. https://doi.org/10.65390/fdiv.2026.136004
  19. Miller, M.A., Pfeiffer, W. & Schwartz, T. (2010) Creating the CIPRES Science Gateway for inference of large phylogenetic trees. 2010 Gateway Computing Environments Workshop (GCE): 1–8. https://doi.org/10.1109/GCE.2010.5676129
  20. Nees von Esenbeck, C.G. (1816) Das System der Pilze und Schwämme. Stahelsche Buchhandlung, Würzburg, 334 pp. https://doi.org/10.5962/bhl.title.46990
  21. Ronquist, F., Teslenko, M., Van Der Mark, P., Ayres, D.L., Darling, A., Höhna, S., Larget, B., Liu, L., Suchard, M.A. & Huelsenbeck, J.P. (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology 61 (3): 539–542. https://doi.org/10.1093/sysbio/sys029
  22. Rossman, A.Y., Adams, G.C., Cannon, P.F., Castlebury, L.A., Crous, P.W., Gryzenhout, M., Jaklitsch, W.M., Mejía, L.C., Stoykov, D., Udayanga, D., Voglmayr, H. & Walker, D.M. (2015) Recommendations of generic names in Diaporthales competing for protection or use. IMA Fungus 6: 145–154. https://doi.org/10.5598/imafungus.2015.06.01.09
  23. Rossman, A.Y., Farr, D.F. & Castlebury, L.A. (2007) A review of the phylogeny and biology of the Diaporthales. Mycoscience 48: 135–144. https://doi.org/10.1007/s10267-007-0347-7
  24. Senanayake, I.C., Crous, P.W., Groenewald, J.Z., Maharachchikumbura, S.S.N., Jeewon, R., Phillips, A.J.L., Bhat, J.D., Perera, R.H., Li, Q.R., Li, W.J., Tangthirasunun, N., Norphanphoun, C., Karunarathna, S.C., Camporesi, E., Manawasighe, I.S., Al-Sadi, A.M. & Hyde, K.D. (2017) Families of Diaporthales based on morphological and phylogenetic evidence. Studies in Mycology 86: 217–296. https://doi.org/10.1016/j.simyco.2017.07.003
  25. Senanayake, I.C., Rathnayaka, A.R., Marasinghe, D.S., Calabon, M.S., Gentekaki, E., Lee, H.B., Hurdeal, V.G., Pem, D., Dissanayake, L.S., Wijesinghe, S.N., Bundhun, D., Nguyen, T.T., Goonasekara, I.D., Abeywickrama, P.D., Bhunjun, C.S., Jayawardena, R.S., Wanasinghe, D.N., Jeewon, R., Bhat, D.J. & Xiang, M.M. (2020) Morphological approaches in studying fungi: Collection, examination, isolation, sporulation and preservation. Mycosphere 11 (1): 2678–2754. https://doi.org/10.5943/mycosphere/11/1/20
  26. Senwanna, C., Hyde, K.D., Phookamsak, R., Jones, E.G. & Cheewangkoon, R. (2018) Coryneum heveanum sp. nov. (Coryneaceae, Diaporthales) on twigs of Para rubber in Thailand. MycoKeys 43: 75–90. https://doi.org/10.3897/mycokeys.43.29365
  27. Stamatakis, A. (2014) RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 30 (9): 1312–1313. https://doi.org/10.1093/bioinformatics/btu033
  28. Stamatakis, A., Hoover, P. & Rougemont, J. (2008) A rapid bootstrap algorithm for the RAxML web servers. Systematic Biology 57 (5): 758–771. https://doi.org/10.1080/10635150802429642
  29. Sutton, B.C. (1975) Coelomycetes V. Coryneum. Mycological Papers 138: 1–224.
  30. U’Ren, J.M., Lutzoni, F., Miadlikowska, J., Laetsch, A.D. & Arnold, A.E. (2012) Host and geographic structure of endophytic and endolichenic fungi at a continental scale. American Journal of Botany 99 (5): 898–914. https://doi.org/10.3732/ajb.1100459
  31. Vilgalys, R. & Hester, M. (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. Journal of Bacteriology 172 (8): 4238–4246. https://doi.org/10.1128/jb.172.8.4238-4246.1990
  32. Voglmayr, H., Akulov, O.Y. & Jaklitsch, W.M. (2016) Reassessment of Allantonectria, phylogenetic position of Thyronectroidea, and Thyronectria caraganae sp. nov. Mycological Progress 15: 921–937. https://doi.org/10.1007/s11557-016-1218-4
  33. Voglmayr, H., Castlebury, L.A. & Jaklitsch, W.M. (2017) Juglanconis gen. nov. on Juglandaceae, and the new family Juglanconidaceae (Diaporthales). Persoonia 38: 136–155. https://doi.org/10.3767/003158517X694768
  34. Voglmayr, H. & Jaklitsch, W.M. (2014) Stilbosporaceae resurrected: generic reclassification and speciation. Persoonia 33: 61–82. https://doi.org/10.3767/003158514X684212
  35. Voglmayr, H., Jaklitsch, W.M., Mohammadi, H. & Chakusary, M.K. (2019) The genus Juglanconis (Diaporthales) on Pterocarya. Mycological Progress 18: 425–437. https://doi.org/10.1007/s11557-018-01464-0
  36. Wang, M., Yang, Q., Yang, W., Shi, L., Zhang, Y., Zhou, Z., Zhang, W. & Zheng, H. (2022) Surface phytolith and pollen assemblages of a low-latitude subtropical region in Southwest China and their implications for vegetation and climate. Frontiers in Plant Science 13: 1007612. https://doi.org/10.3389/fpls.2022.1007612
  37. White, T.J., Bruns, T., Lee, S. & Taylor, J. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis, M.A., Gelfand, D.H., Sninsky, J.J. & White, T.J. (Eds.) PCR protocols: A guide to methods and applications. Academic Press, San Diego, pp. 315–322. https://doi.org/10.1016/b978-0-12-372180-8.50042-1
  38. Wijayawardene, N.N., Hyde, K.D., Rajeshkumar, K.C., Hawksworth, D.L., Madrid, H., Kirk, P.M., Braun, U., Singh, R.V., Crous, P.W., Kukwa, M., Lücking, R., Kurtzman, C.P., Yurkov, A., Haelewaters, D., Aptroot, A., Lumbsch, H.T., Timdal, E., Ertz, D., Etayo, J., Phillips, A.J.L., Groenewald, J.Z., Papizadeh, M., Selbmann, L., Dayarathne, M.C., Weerakoon, G., Jones, E.B.G., Suetrong, S., Tian, Q., Castañeda-Ruiz, R.F., Bahkali, A.H., Pang, K., Tanaka, K., Dai, D.Q., Sakayaroj, J., Hujslová, M., Lombard, L., Shenoy, B.D., Suija, A., Maharachchikumbura, S.S.N., Thambugala, K.M., Wanasinghe, D.N., Sharma, D.O., Gaikwad, S., Pandit, G., Zucconi, L., Onofri, S., Egidi, E., Raja, H.A., Kodsueb, R., Cáceres, M.E.S., Pérez-Ortega, S., Fiuza, P.O., Monteiro, J.S., Vasilyeva, L.N., Shivas, R.G., Prieto, M., Wedin, M., Olariaga, I., Lateef, A.A., Agrawal, Y., Fazeli, S.A.S., Amoozegar, M.A., Zhao, G.Z., Pfliegler, W.P., Sharma, G., Oset, M., Abdel-Wahab, M.A., Takamatsu, S., Bensch, K., de Silva, N.I., De Kesel, A., Karunarathna, A., Boonmee, S., Pfister, D.H., Lu, Y., Luo, Z., Boonyuen, N., Daranagama, D.A., Senanayake, I.C., Jayasiri, S.C., Samarakoon, M.C., Zeng, X., Doilom, M., Quijada, L., Rampadarath, S., Heredia, G., Dissanayake, A.J., Jayawardana, R.S., Perera, R.H., Tang, L.Z., Phukhamsakda, C., Hernández-Restrepo, M., Ma, X., Tibpromma, S., Gusmão, L.F.P., Weerahewa, D. & Karunarathna, S.C. (2017) Notes for genera: Ascomycota. Fungal Diversity 86: 1–594. https://doi.org/10.1007/s13225-017-0386-0
  39. Wijayawardene, N.N., Hyde, K.D., Wanasinghe, D.N., Papizadeh, M., Goonasekara, I.D., Camporesi, E., Bhat, D.J., McKenzie, E.H.C., Phillips, A.J.L., Diederich, P., Tanaka, K., Li, W.J., Tangthirasunun, N., Phookamsak, R., Dai, D.Q., Dissanayake, A.J., Weerakoon, G., Maharachchikumbura, S.S.N., Hashimoto, A., Matsumura, M., Bahkali, A.H. & Wang, Y. (2016) Taxonomy and phylogeny of dematiaceous coelomycetes. Fungal Diversity 77: 1–136. https://doi.org/10.1007/s13225-016-0360-2
  40. Xiang, C., Gao, F., Jakovlić, I., Lei, H., Hu, Y., Zhang, H., Zou, H., Wang, G. & Zhang, D. (2023) Using PhyloSuite for molecular phylogeny and tree-based analyses. iMeta 2 (1): e87. https://doi.org/10.1002/imt2.87
  41. Zhan, C., Wei, R., Zhao, L., Chen, S. & Shen, C. (2025) Optimal distribution modeling and multifractal analysis of wind speed in the complex terrain of Sichuan Province, China. Scientific Reports 15: 4648. https://doi.org/10.1038/s41598-024-83798-2
  42. Zhang, D., Gao, F., Jakovlić, I., Zou, H., Zhang, J., Li, W.X. & Wang, G.T. (2020) PhyloSuite: An integrated and scalable desktop platform for streamlined molecular sequence data management and evolutionary phylogenetics studies. Molecular Ecology Resources 20 (1): 348–355. https://doi.org/10.1111/1755-0998.13096
  43. Zhang, Y., Xia, J., Yang, F., She, D., Zou, L., Hong, S., Wang, Q., Yuan, F. & Song, L. (2023) Analysis of Drought Characteristic of Sichuan Province, Southwestern China. Water 15 (8): 1601. https://doi.org/10.3390/w15081601
  44. Zhu, H.Y., Tian, C.M. & Fan, X.L. (2018) Multigene phylogeny and morphology reveal Cytospora spiraeae sp. nov. (Diaporthales, Ascomycota) in China. Phytotaxa 338 (1): 49–62. https://doi.org/10.11646/phytotaxa.338.1.4

How to Cite

Deng, L.-P., Ding, P.-C., Ismail, A.M. & Maharachchikumbura, S.S.N. (2026) Coryneum chengduense sp. nov. (Coryneaceae, Diaporthales) from Sichuan Province, China. Phytotaxa 774 (1): 49–59. https://doi.org/10.11646/phytotaxa.774.1.4