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Issue: Vol. 756 No. 2: 14 May 2026
Type: Article
Published: 2026-05-14
DOI: 10.11646/phytotaxa.756.2.3
Page range: 138-150
Abstract views: 0
PDF downloaded: 0

Morphology and multi-locus phylogeny reveal a new Rhamphoriopsis species from Guizhou Province, China

College of Biological Science and Food Engineering, Southwest Forestry University, Kunming 650224, China; Modern Industry School of Edible Fungi, Southwest Forestry University, Kunming 650224, China
College of Forestry, Southwest Forestry University, Kunming 650224, China
College of Forestry, Southwest Forestry University, Kunming 650224, China
Modern Industry School of Edible Fungi, Southwest Forestry University, Kunming 650224, China; College of Forestry, Southwest Forestry University, Kunming 650224, China
College of Biological Science and Food Engineering, Southwest Forestry University, Kunming 650224, China; Modern Industry School of Edible Fungi, Southwest Forestry University, Kunming 650224, China
1 new species microfungi Phaeoisaria-like fungi Rhamphoriaceae taxonomy Fungi

Abstract

During a mycological survey conducted in Zhenyuan County, Qiandongnan Miao and Dong Autonomous Prefecture, Guizhou Province, China, a Phaeoisaria-like fungus was collected from decayed wood in a terrestrial habitat. Multi-locus phylogenetic analyses of ITS, LSU, SSU, rpb2, and tef1-α sequence data revealed that the taxon forms an independent lineage within Rhamphoriopsis, sister to R. yunnanensis. Based on morphology and multi-gene phylogeny, the fungus is described herein as a new species, Rhamphoriopsis guizhouensis. A comprehensive description, illustration, and phylogenetic analysis results showing the placement of the new species are provided. This study contributes to a better understanding of the genus Rhamphoriopsis and expands the knowledge of fungal diversity in Guizhou Province, China.

References

  1. Capella-Gutiérrez, S., Silla-Martínez, J.M. & Gabaldón, T. (2009) TrimAl: A tool for automated alignment trimming in large-scale phylogenetic analyses. Bioinformatics 25: 1972–1973. https://doi.org/10.1093/bioinformatics/btp348
  2. Carbone, I. & Kohn, L.M. (1999) A method for designing primer sets for speciation studies in filamentous ascomycetes. Mycologia 91: 553–556. https://doi.org/10.1080/00275514.1999.12061051
  3. Chethana, T.K.W., Manawasinghe, I.S., Hurdeal, V.G., Bhunjun, C.S., Appadoo, M.A., Gentekaki, E., Raspé, O., Promputtha, I. & Hyde, K.D. (2021) What are fungal species and how to delineate them? Fungal Diversity 109: 1–25. https://doi.org/10.1007/s13225-021-00483-9
  4. Crous, P.W., Costa, M.M., Kandemir, H., Vermaas, M., Vu, D., Zhao, L., Arumugam, E., Flakus, A., Jurjević, Ž., Kaliyaperumal, M., Mahadevakumar, S., Murugadoss, R., Shivas, R.G., Tan, Y.P., Wingfield, M.J., Abell, S.E., Marney, T.S., Danteswari, C., Darmostuk, V., Denchev, C.M., Denchev, T.T., Etayo, J., Gené, J., Gunaseelan, S., Hubka, V., Illescas, T., Jansen, G.M., Kezo, K., Kumar, S., Larsson, E., Mufeeda, K.T., Piątek, M., Rodriguez-Flakus, P., Sarma, P.V.S.R.N., Stryjak-Bogacka, M., Torres-Garcia, D., Vauras, J., Acal, D.A., Akulov, A., Alhudaib, K., Asif, M., Balashov, S., Baral, H.-O., Baturo-Cieśniewska, A., Begerow, D., Beja-Pereira, A., Bianchinotti, M.V., Bilański, P., Chandranayaka, S., Chellappan, N., Cowan, D.A., Custódio, F.A., Czachura, P., Delgado, G., De Silva, N.I., Dijksterhuis, J., Dueñas, M., Eisvand, P., Fachada, V., Fournier, J., Fritsche, Y., Fuljer, F., Ganga, K.G.G., Guerra, M.P., Hansen, K., Hywel-Jones, N., Ismail, A.M., Jacobs, C.R., Jankowiak, R., Karich, A., Kemler, M., Kisło, K., Klofac, W., Krisai-Greilhuber, I., Latha, K.P.D., Lebeuf, R., Lopes, M.E., Lumyong, S., Maciá-Vicente, J.G., Maggs-Kölling, G., Magistà, D., Manimohan, P., Martín, M.P., Mazur, E., Mehrabi-Koushki, M., Miller, A.N., Mombert, A., Ossowska, E.A., Patejuk, K., Piskorski, S., Plaza, M., Podile, A.R., Polhorský, A., Pusz, W., Raza, M., Ruszkiewicz-Michalska, M., Saba, M., Sánchez, R.M., Singh, R., Śliwa, L., Smith, M.E., Stefenon, V.M., Strašiftáková, D., Suwannarach, N., Szczepańska, K., Telleria, M.T., Tennakoon, D.S., Thines, M., Thorn, R.G., Urbaniak, J., van der Vegte, M., Vasan, V., Vila-Viçosa, C., Voglmayr, H., Wrzosek, M., Zappelini, J. & Groenewald, J.Z. (2023) Fungal Planet description sheets: 1550–1613. Persoonia 51: 280–417. https://doi.org/10.3767/persoonia.2023.51.08
  5. de Hoog, G.S. & Papendorf, M.C. (1976) The genus Phaeoisaria. Persoonia 8: 407–414.
  6. Glez-Peña, D., Gómez-Blanco, D., Reboiro-Jato, M., Fdez-Riverola, F. & Posada, D. (2010) ALTER: program-oriented conversion of DNA and protein alignments. Nucleic Acids Research 38: 14–18. https://doi.org/10.1093/nar/gkq321
  7. Hall, T.A. (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 41: 95–98. https://doi.org/10.1021/bk-1999-0734.ch008
  8. Höhnel, F. (1909) Fragmente zur Mykologie VI. Mitteilung, Nr. 182 bis 288. Sitzungsber. Kaiserliche Akademie der Wissenschaften in Wien, Mathematisch-Naturwissenschaftliche Klasse. Abteilung I 118: 275–452. https://doi.org/10.1007/978-3-662-24755-6_1
  9. Hughes, S.J. (1958) Revisiones hyphomycetum aliquot cum appendice de nominibus rejiciendis. Canadian Journal of Botany 36: 727–836. https://doi.org/10.1139/b58-06
  10. Hyde, K.D., Norphanphoun, C., Maharachchikumbura, S.S.N., Bhat, D.J., Jones, E.B.G., Bundhun, D., Chen, Y.J., Bao, D.F., Boonmee, S., Calabon, M.S., Chaiwan, N., Chethana, K.W.T., Dai, D.Q., Dayarathne, M.C., Devadatha, B., Dissanayake, A.J., Dissanayake, L.S., Doilom, M., Dong, W., Fan, X.L., Goonasekara, I.D., Hongsanan, S., Huang, S.K., Jayawardena, R.S., Jeewon, R., Karunarathna, A., Konta, S., Kumar, V., Lin, C.G., Liu, J.K., Liu, N.G., Luangsa-ard, J., Lumyong, S., Luo, Z.L., Marasinghe, D.S., McKenzie, E.H.C., Niego, A.G.T., Niranjan, M., Perera, R.H., Phukhamsakda, C., Rathnayaka, A.R., Samarakoon, M.C., Samarakoon, S.M.B.C., Sarma, V.V., Senanayake, I.C., Shang, Q.J., Stadler, M., Tibpromma, S., Wanasinghe, D.N., Wei, D.P., Wijayawardene, N.N., Xiao, Y.P., Yang, J., Zeng, X.Y., Zhang, S.N. & Xiang, M.M. (2020a) Refined families of Sordariomycetes. Mycosphere 11 (1): 305–1059. https://doi.org/10.5943/mycosphere/11/1/7
  11. Hyde, K.D., Jeewon, R., Chen, Y.J., Bhunjun, C.S., Calabon, M.S., Jiang, H.B., Lin, C.G., Norphanphoun, C., Sysouphanthong, P., Pem, D., Tibpromma, S., Zhang, Q., Doilom, M., Jayawardena, R.S., Liu, J.K., Maharachchikumbura, S.S.N., Phukhamsakda, C., Phookamsak, R., Al-Sadi, A.M., Thongklang, N., Wang, Y., Gaforov, Y., Jones, E.B.G. & Lumyong, S. (2020b) The numbers of fungi: is the descriptive curve lattening? Fungal Diversity 103: 219–271. https://doi.org/10.1007/s13225-020-00458-2
  12. Hyde, K.D., Bao, D.F., Hongsanan, S., Chethana, K.W.T., Yang, J. & Suwannarach, N. (2021) Evolution of freshwater Diaporthomycetidae (Sordariomycetes) provides evidence for five new orders and six new families. Fungal Diversity 107 (1): 71–105. https://doi.org/10.1007/s13225-021-00469-7
  13. Hyde, K.D., Maharachchikumbura, S.S., Hongsanan, S., Samarakoon, M.C., Lucking, R., Pem, D., Harishchandra, D., Jeewon, R., Zhao, R.L., Xu, J.C. & Liu, J.K. (2017) The ranking of fungi: a tribute to David L. Hawksworth on his 70th birthday. Fungal Diversity 84: 1–23. https://doi.org/10.1007/s13225-017-0383-3
  14. Hyde, K.D., Noorabadi, M.T., Thiyagaraja, V., He, M.Q., Johnston, P.R., Wijesinghe, S.N., Armand, A., Biketova, A.Y., Chethana, K.W.T., Erdoğdu, M., Ge, Z.W., Groenewald, J.Z., Hongsanan, S., Kušan, I., Leontyev, D.V., Li, D.W., Lin, C.G., Liu, N.G., Maharachchikumbura, S.S.N., Matočec, N., May, T.W., McKenzie, E.H.C., Mešić, A., Perera, R.H., Phukhamsakda, C., Piątek, M., Samarakoon, M.C., Selcuk, F., Senanayake, I.C., Tanney, J.B., Tian, Q., Vizzini, A., Wanasinghe, D.N., Wannasawang, N., Wijayawardene, N.N., Zhao, R.L., Abdel-Wahab, M.A., Abdollahzadeh, J., Abeywickrama, P.D., Abhinav, Absalan, S., Acharya, K., Afshari, N., Afshan, N.S., Afzalinia, S., Ahmadpour, S.A., Akulov, O., Alizadeh, A., Alizadeh, M., Al-Sadi, A.M., Alves, A., Alves, V.C.S., Alves-Silva, G., Antonín, V., Aouali, S., Aptroot, A., Apurillo, C.C.S., Arias, R.M., Asgari, B., Asghari, R., Assis, D.M.A., Assyov, B., Atienza, V., Aumentado, H.D.R., Avasthi, S., Azevedo, E., Bakhshi, M., Bao, D.F., Baral, H.O., Barata, M., Barbosa, K.D., Barbosa, R.N., Barbosa, F.R., Baroncelli, R., Barreto, G.G., Baschien, C., Bennett, R.M., Bera, I., Bezerra, J.D.P., Bhunjun, C.S., Bianchinotti, M.V., Błaszkowski, J., Boekhout, T., Bonito, G.M., Boonmee, S., Boonyuen, N., Bortnikov, F.M., Bregant, C., Bundhun, D., Burgaud, G., Buyck, B., Caeiro, M.F., Cabarroi-Hernández, M., Cai, M.F., Cai, L., Calabon, M.S., Calaça, F.J.S., Callalli, M., Câmara, M.P.S., Cano-Lira, J., Cao, B., Carlavilla, J.R., Carvalho, A., Carvalho, T.G., Castañeda-Ruiz, R.F., Catania, M.D.V., Cazabonne, J., Cedeño-Sanchez, M., Chaharmiri-Dokhaharani, S., Chaiwan, N., Chakraborty, N., Cheewankoon, R., Chen, C., Chen, J., Chen, Q., Chen, Y.P., Chinaglia, S., Coelho-Nascimento, C.C., Coleine, C., Costa-Rezende, D.H., Cortés-Pérez, A., Crouch, J.A., Crous, P.W., Cruz, R.H.S.F., Czachura, P., Damm, U., Darmostuk, V., Daroodi, Z., Das, K., Das, K., Davoodian, N., Davydov, E.A., da Silva, G.A., da Silva, I.R., da Silva, R.M.F., da Silva Santos, A.C., Dai, D.Q., Dai, Y.C., de Groot, M.D., De Kesel, A., De Lange, R., de Medeiros, E.V., de Souza, C.F.A., de Souza, F.A., dela Cruz, T.E.E., Decock, C., Delgado, G., Denchev, C.M., Denchev, T.T., Deng, Y.L., Dentinger, B.T.M., Devadatha, B., Dianese, J.C., Dima, B., Doilom, M., Dissanayake, A.J., Dissanayake, D.M.L.S., Dissanayake, L.S., Diniz, A.G., Dolatabadi, S., Dong, J.H., Dong, W., Dong, Z.Y., Drechsler-Santos, E.R., Druzhinina, I.S., Du, T.Y., Dubey, M.K., Dutta, A.K., Elliott, T.F., Elshahed, M.S., Egidi, E., Eisvand, P., Fan, L., Fan, X., Fan, X.L., Fedosova, A.G., Ferro, L.O., Fiuza, P.O., Flakus, A., Fonseca, E.O.W., Fryar, S.C., Gabaldón, T., Gajanayake, A.J., Gannibal, P.B., Gao, F., García-Sánchez, D., García-Sandoval, R., Garrido-Benavent, I., Garzoli, L., Gasca-Pineda, J., Gautam, A.K., Gené, J., GhobadNejhad, M., Ghosh, A., Giachini, A.J., Gibertoni, T.B., Gentekaki, E., Gmoshinskiy, V.I., Góes-Neto, A., Gomdola, D., Gorjón, S.P., Goto, B.T., Granados-Montero, M.M., Griffith, G.W., Groenewald, M., Grossart, H.P., Gu, Z.R., Gueidan, C., Gunarathne, A., Gunaseelan, S., Guo, S.L., Gusmão, L.F.P., Gutierrez, A.C., Guzmán-Dávalos, L., Haelewaters, D., Haituk, H., Halling, R.E., He, S.C., Heredia, G., Hernández-Restrepo, M., Hosoya, T., Hoog, S.D., Horak, E., Hou, C.L., Houbraken, J., Htet, Z.H., Huang, S.K., Huang, W.J., Hurdeal, V.G., Hustad, V.P., Inácio, C.A., Janik, P., Jayalal, R.G.U., Jayasiri, S.C., Jayawardena, R.S., Jeewon, R., Jerônimo, G.H., Jin, J., Jones, E.B.G., Joshi, Y., Jurjević, Ž., Justo, A., Kakishima, M., Kaliyaperumal, M., Kang, G.P., Kang, J.C., Karimi, O., Karunarathna, S.C., Karpov, S.A., Kezo, K., Khalid, A.N., Khan, M.K., Khuna, S., Khyaju, S., Kirchmair, M., Klawonn, I., Kraisitudomsook, N., Kukwa, M., Kularathnage, N.D., Kumar, S., Lachance, M.A., Lado, C., Latha, K.P.D., Lee, H.B., Leonardi, M., Lestari, A.S., Li, C., Li, H., Li, J., Li, Q., Li, Y., Li, Y.C., Li, Y.X., Liao, C.F., Lima, J.L.R., Lima, J.M.S., Lima, N.B., Lin, L., Linaldeddu, B.T., Linn, M.M., Liu, F., Liu, J.K., Liu, J.W., Liu, S., Liu, S.L., Liu, X.F., Liu, X.Y., Longcore, J.E., Luangharn, T., Luangsa-ard, J.J., Lu, L., Lu, Y.Z., Lumbsch, H.T., Luo, L., Luo, M., Luo, Z.L., Ma, J., Madagammana, A.D., Madhushan, A., Madrid, H., Magurno, F., Magyar, D., Mahadevakumar, S., Malosso, E., Malysh, J.M., Mamarabadi, M., Manawasinghe, I.S., Manfrino, R.G., Manimohan, P., Mao, N., Mapook, A., Marchese, P., Marasinghe, D.S., Mardones, M., Marin-Felix, Y., Masigol, H., Mehrabi, M., Mehrabi-Koushki, M., Meiras-Ottoni, A. de, Melo, R.F.R., Mendes-Alvarenga, R.L., Mendieta, S., Meng, Q.F., Menkis, A., Menolli, N. Jr., Mikšík, M., Miller, S.L., Moncada, B., Moncalvo, J.M., Monteiro, J.S., Monteiro, M., Mora-Montes, H.M., Moroz, E.L., Moura, J.C., Muhammad, U., Mukhopadhyay, S., Nagy, G.L., Najam ul Sehar, A., Najafiniya, M., Nanayakkara, C.M., Naseer, A., Nascimento, E.C.R., Nascimento, S.S., Neuhauser, S., Neves, M.A., Niazi, A.R., Nie, Y., Nilsson, R.H., Nogueira, P.T.S., Novozhilov, Y.K., Noordeloos, M., Norphanphoun, C., Nuñez Otaño, N., O’Donnell, R.P., Oehl, F., Oliveira, J.A., Oliveira Junior, I., Oliveira, N.V.L., Oliveira, P.H.F., Orihara, T., Oset, M., Pang, K.L., Papp, V., Pathirana, L.S., Peintner, U., Pem, D., Pereira, O.L., Pérez-Moreno, J., Pérez-Ortega, S., Péter, G., Pires-Zottarelli, C.L.A., Phonemany, M., Phongeun, S., Pošta, A., Prazeres, J.F.S.A., Quan, Y., Quandt, C.A., Queiroz, M.B., Radek, R., Rahnama, K., Raj, K.N.A., Rajeshkumar, K.C., Rajwar, S., Ralaiveloarisoa, A.B., Rämä, T., Ramírez-Cruz, V., Rambold, G., Rathnayaka, A.R., Raza, M., Ren, G.C., Rinaldi, A.C., Rivas-Ferreiro, M., Robledo, G.L., Ronikier, A., Rossi, W., Rusevska, K., Ryberg, M., Safi, A., Salimi, F., Salvador-Montoya, C.A., Samant, B., Samaradiwakara, N.P., Sánchez-Castro, I., Sandoval-Denis, M., Santiago, A.L.C.M.A., Santos, A.C.D.S., Santos, L.A. dos, Sarma, V.V., Sarwar, S., Savchenko, A., Savchenko, K., Saxena, R.K., Schoutteten, N., Selbmann, L., Ševčíková, H., Sharma, A., Shen, H.W., Shen, Y.M., Shu, Y.X., Silva, H.F., Silva-Filho, A.G.S., Silva, V.S.H., Simmons, D.R., Singh, R., Sir, E.B., Sohrabi, M., Souza, F.A., SouzaMotta, C.M., Sriindrasutdhi, V., Sruthi, O.P., Stadler, M., Stemler, J., Stephenson, S.L., Stoyneva-Gaertner, M.P., Strassert, J.F.H., Stryjak-Bogacka, M., Su, H., Sun, Y.R., Svantesson, S., Sysouphanthong, P., Takamatsu, S., Tan, T.H., Tanaka, K., Tang, C., Tang, X., Taylor, J.E., Taylor, P.W.J., Tennakoon, D.S., Thakshila, S.A.D., Thambugala, K.M., Thamodini, G.K., Thilanga, D., Thines, M., Tiago, P.V., Tian, X.G., Tian, W.H., Tibpromma, S., Tkalčec, Z., Tokarev, Y.S., Tomšovský, M., Torruella, G., Tsurykau, A., Udayanga, D., Ulukapı, M., Untereiner, W.A., Usman, M., Uzunov, B.A., Vadthanarat, S., Valenzuela, R., Van den Wyngaert, S., Van Vooren, N., Velez, P., Verma, R.K., Vieira, L.C., Vieira, W.A.S., Vinzelj, J.M., Tang, A.M.C., Walker, A., Walker, A.K., Wang, Q.M., Wang, Y., Wang, X.Y., Wang, Z.Y., Wannathes, N., Wartchow, F., Weerakoon, G., Wei, D.P., Wei, X., White, J.F., Wijesundara, D.S.A., Wisitrassameewong, K., Worobiec, G., Wu, H.X., Wu, N., Xiong, Y.R., Xu, B., Xu, J.P., Xu, R., Xu, R.F., Xu, R.J., Yadav, S., Yakovchenko, L.S., Yang, H.D., Yang, X., Yang, Y.H., Yang, Y., Yang, Y.Y., Yoshioka, R., Youssef, N.H., Yu, F.M., Yu, Z.F., Yuan, L.L., Yuan, Q., Zabin, D.A., Zamora, J.C., Zapata, C.V., Zare, R., Zeng, M., Zeng, X.Y., Zhang, J.F., Zhang, J.Y., Zhang, S., Zhang, X.C., Zhao, C.L., Zhao, H., Zhao, Q., Zhao, H., Zhao, H.J., Zhou, H.M., Zhu, X.Y., Zmitrovich, I.V., Zucconi, L. & Zvyagina, E. (2024) The 2024 outline of fungi and fungus-like taxa. Mycosphere 15 (1): 5146–6239. https://doi.org/10.5943/mycosphere/15/1/25
  15. Jayasiri, S.C., hyde, K.D., Ariyawansa, h.A., Bhat, J., Buyck, B., Cai, L., Dai, Y.C., Abd-Elsalam, K.A., Ertz, D., hidayat, I., Jeewon, R., Jones, E.B.G., Bahkali, A.h., Karunarathna, S.C., Liu, J.K., Luangsa-ard, J.J., Lumbsch, h.T., Maharachchikumbura, S.S.N., McKenzie, E.h.C., Moncalvo, J.M., Ghobad-Nejhad, M., Nilsson, h., Pang, K.A., Pereira, O.L., Phillips, A.J.L., Raspé, O., Rollins, A.W., Romero, A.I., Etayo, J., Selçuk, F., Stephenson, S.L., Suetrong, S., Taylor, J.E., Tsui, C.K.M., Vizzini, A., Abdel-Wahab, M.A., Wen, T.C., Boonmee, S., Dai, D.Q., Daranagama, D.A., Dissanayake, A.J., Ekanayaka, A.h., Fryar, S.C., hongsanan, S., Jayawardena, R.S., Li, W.J., Perera, R.h., Phookamsak, R., de Silva, N.I., Thambugala, K.M., Tian, Q., Wijayawarden, N.N., Zhao, R.L., Zhao, Q., Kang, J.C. & Promputtha, I. (2015) The Faces of Fungi database: fungal names linked with morphology, phylogeny and human impacts. Fungal Diversity 74: 3–18. https://doi.org/10.1007/s13225-015-0351-8
  16. Katoh, K. & Standley, D.M. (2016) A simple method to control over-alignment in the MAFFT multiple sequence alignment program. Bioinformatics 32: 1933–1942. https://doi.org/10.1093/bioinformatics/btw108
  17. Larsson, A. (2014) AliView: a fast and lightweight alignment viewer and editor for large data sets. Bioinformatics 30: 3276–3278. https://doi.org/10.1093/bioinformatics/btu531
  18. Lin, C.G., Liu, J.K., Chukeatirote, E. & Hyde, K.D. (2023) Rhamphoriopsis hyalospora sp. nov. associated with decaying wood from China. Phytotaxa 598 (3): 245–253. https://doi.org/10.11646/phytotaxa.598.3.6
  19. Lin, C.G., Hyde, K.D., Feng, Y., Xiao, Y.P., Liu, N.G., Lu, Y.Z., Luo, Z.L. & Liu, J.K. (2025) Notes, outline, systematics and phylogeny of hyaline-spored hyphomycetes. Fungal Diversity 135: 57–467. https://doi.org/10.1007/s13225-025-00561-2
  20. Liu, L.L., Zhang, Q.F., Li, W.M., Habib, K., Ren, Y.L., Luo, X.Y., Lu, C.T., Wijayawardene, N.N., Elgorban, A.M., Kyaw, H.W.W., Bashir, H., Al-Shwaiman, H.A., Samarakoon, M.C., Li, Q.R. & Liu, H.M. (2025) Diversity and taxonomy of hyphomycetous fungi in southwestern China. Mycosphere 16: 3951–4062. https://doi.org/10.5943/mycosphere/16/1/31
  21. Liu, Y.J., Whelen, S. & Hall, B.D. (1999) Phylogenetic relationships among ascomycetes: evidence from an RNA polymerase II subunit. Molecular Biology and Evolution 16: 1799–1808. https://doi.org/10.1093/oxfordjournals.molbev.a026092
  22. Ma, J., Zhang, L.J., Boonmee, S., Xiao, X.J., Liu, N.G., Xiao, Y.P., Luo, Z.L. & Lu, Y.Z. (2023) Morphological and phylogenetic analyses reveal three new species and one new record of Tubeufia (Tubeufiales, Tubeufiaceae) from southern China. MycoKeys 99: 87–108. https://doi.org/10.3897/mycokeys.99.107606
  23. Miller, M.A., Pfeiffer, W. & Schwartz, T. (2010) Creating the CIPRES Science Gateway for inference of large phylogenetic trees. In: Proceedings of the gateway computing environments workshop (GCE). Institute of Electrical and Electronics Engineers, New Orleans, pp. 1–8. https://doi.org/10.1109/GCE.2010.5676129
  24. Müller, E. & Samuels, G.J. (1982) Anamorphs of pyrenomycetous Ascomycetes I. Rhamphoria Niessl and Trichosphaerella Bommer, Rousseau & Saccardo. Sydowia 35: 143–149.
  25. Nylander, J.A.A. (2004) MrModeltest V2. Program distributed by the author. In Evolutionary Biology Centre; Uppsala University: Uppsala, Sweden.
  26. Pitakpattanakul, C., Han, M.Y., Karunarathana, S.C., Lu, W.H., Zheng, D.G., Chia, P., Bunphan, J., Dai, D.Q., Elgorban, A.M., Chukeatirote, E. & Tibpromma, S. (2025) Morphology and multi-locus phylogeny reveal a new coffee-associated saprobic Rhamphoriopsis (Rhamphoriaceae, Rhamphoriales) species in Yunnan Province, China. Phytotaxa 725 (1): 41–55. https://doi.org/10.11646/phytotaxa.725.1.4
  27. Rannala, B. & Yang, Z. (1996) Probability distribution of molecular evolutionary trees: A new method of phylogenetic inference. Journal of Molecular Evolution 43 (3): 304–311. https://doi.org/10.1007/BF02338839
  28. Rathnayaka, A.R., Tennakoon, D.S., Jones, G.E., Wanasinghe, D.N., Bhat, D.J., Priyashantha, A.H., Stephenson, S.L., Tibpromma, S. & Karunarathna, S.C. (2024) Significance of precise documentation of hosts and geospatial data of fungal collections, with an emphasis on plant-associated fungi. New Zealand Journal of Botany 63 (2–3): 462–489. https://doi.org/1000.1080/0028825X.2024.2381734
  29. Réblová, M., Nekvindová, J., Hernández-Restrepo, M., Hradilová, M. & Kolařík, M. (2025) Phylogeny, taxonomy and geographic distribution of novel and known fungi with holoblastic-denticulate conidiogenesis in Rhamphoriales and Pleurotheciales (Sordariomycetes). Persoonia 55: 277–311. https://doi.org/10.3114/persoonia.2025.55.08
  30. Réblová, M. & Štěpánek, V. (2018) Introducing the Rhamphoriaceae, fam. nov. (Sordariomycetes), two new genera, and new life histories for taxa with Phaeoisaria- and Idriella-like anamorphs. Mycologia 11 (4): 750–770. https://doi.org/10.1080/00275514.2018.1475164
  31. Richard, M.D. & Lippmann, R.P. (1991) Neural network classifiers estimate Bayesian a posteriori probabilities. Neural Computation 3 (4): 461–483. https://doi.org/10.1162/neco.1991.3.4.461
  32. Ronquist, F., Teslenko, M., Van Der Mark, P., Ayres, D.L., Darling, A., Höhna, S., Larget, B., Liu, L., Suchard, M.A. & Huelsenbeck, J.P. (2012) MrBayes 3.2: Efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology 61 (3): 539–542. https://doi.org/10.1093/sysbio/sys029
  33. Seifert, K.A. & Gams, W. (2011) The genera of Hyphomycetes – 2011 update. Persoonia 27: 119–129. https://doi.org/10.3767/003158511X617435
  34. Senanayake, I.C., Rathnayaka, A.R., Marasinghe, D.S., Calabon, M.S., Gentekaki, E., Lee, H.B., Hurdeal, V.G., Pem, D., Dissanayake, L.S., Wijesinghe, S.N., Bundhun, D., Nguyen, T.T., Goonasekara, I.D., Abeywickrama, P.D., Bhunjun, C.S., Jayawardena, R.S., Wanasinghe, D.N., Jeewon, R., Bhat, D.J. & Xiang, M.M. (2020) Morphological approaches in studying fungi: collection, examination, isolation, sporulation and preservation. Mycosphere 11 (1): 2678–2754. https://doi.org/10.5943/mycosphere/11/1/20
  35. Shenoy, B.D., Jeewon, R., Wu, W.P., Bhat, D.J. & Hyde, K.D. (2006) Ribosomal and RPB2 DNA sequence analyses suggest that Sporidesmium and morphologically similar genera are polyphyletic. Mycological Research 110: 916–928. https://doi.org/10.1016/j.mycres.2006.06.004
  36. Stamatakis, A. (2014) RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 30: 1312–1313. https://doi.org/10.1093/bioinformatics/btu033
  37. Stamatakis, A., Hoover, P. & Rougemont, J. (2008) A rapid bootstrap algorithm for the RAxML web servers. Systematic biology 57: 758–771. https://doi.org/10.1080/10635150802429642
  38. Vaidya, G., Lohman, D.J. & Meier, R. (2011) SequenceMatrix: Concatenation software for the fast assembly of multi-gene datasets with character set and codon information. Cladistics: The International Journal of the Willi Hennig Society 27: 171–180. https://doi.org/10.1111/j.1096-0031.2010.00329.x
  39. Vilgalys, R. & Hester, M. (1990) Rapid genetic identification and mapping of enzymatically amplified 593 ribosomal DNA from several Cryptococcus species. Journal of Bacteriology 172: 4238–4246. https://doi.org/10.1128/jb.172.8.4238-4246.1990
  40. White, T.J., Bruns, T., Lee, S.J.W.T. & Taylor, J.L. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR protocols: a guide to methods and applications. Academic Press, San Diego, pp. 315–322. https://doi.org/10.1016/B978-0-12-372180-8.50042-1
  41. Xiao, X.J., Liu, N.G., Liu, J.K., Shen, H.W., Yu, Y.X., Yu, X.D., Xiao, Y., Tian, X.G., Han, M.Y., Bao, D.F., Liu, J.W., Yang, Y., Ma, J., Zhang, J.Y., Sun, Y.R., Luo, Z.L., Hongsanan, S., Xiao, Y.P., Jayawardena, R.S., Lu, Y.Z. & Sun, J.Z. (2025) Fungal Diversity of Ascomycota in China and Thailand: Descriptions of New Taxa in Honour of Kevin D. Hyde’s 70th Birthday. Mycosphere 16 (2): 344–415. https://doi.org/10.5943/mycosphere/16/2/3
  42. Yang, J., Liu, L.L., Jones, E.B.G., Hyde, K.D., Liu, Z.Y., Bao, D.F., Liu, N.G., Li, W.L., Shen, H.W., Yu, X.D. & Liu, J.K. (2023a) Freshwater fungi from karst landscapes in China and Thailand. Fungal Diversity 119: 1–212. https://doi.org/10.1007/s13225-023-00514-7
  43. Yang, J.X., Zhang, H., Wang, L., Zhu, R., Dong, W. & Chen, S.Y. (2023b) Phaeoisaria ellipsoidea (Pleurotheciaceae, Pleurotheciales, Sordariomycetes), a new lignicolous freshwater fungus from Xishuangbanna, Yunnan Province, China. Phytotaxa 591 (1): 19–30. https://doi.org/10.11646/phytotaxa.591.1.2
  44. Zeng, X.Y., Tan, T.J., Tian, F.H., Wang, Y. & Wen, T.C. (2023) OFPT: A one-stop software for fungal phylogeny. Mycosphere 14 (1): 1730–1741. https://doi.org/10.5943/mycosphere/14/1/20
  45. Zhaxybayeva, O. & Gogarten, J.P. (2002) Bootstrap, Bayesian probability and maximum likelihood mapping: Exploring new tools for comparative genome analyses. BMC Genomics 3 (1): 4. https://doi.org/10.1186/1471-2164-3-4

How to Cite

Liu, X.-F., Chen, M., Ye, S.-Y., Zhao, C.-L. & Shen, S. (2026) Morphology and multi-locus phylogeny reveal a new Rhamphoriopsis species from Guizhou Province, China. Phytotaxa 756 (2): 138–150. https://doi.org/10.11646/phytotaxa.756.2.3