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Issue: Vol. 712 No. 1: 29 Jul. 2025
Type: Article
Published: 2025-07-29
DOI: 10.11646/phytotaxa.712.1.5
Page range: 59-66
Abstract views: 258
PDF downloaded: 9

Tuber guizhouense sp. nov. (Tuberaceae, Ascomycota) from Guizhou Province, China

Guizhou Institute of Biology, Guizhou Academy of Sciences, 1 Longjiang Lane, Guiyang, China; Ministry of Education Key Laboratory for Ecology of Tropical Islands, Key Laboratory of Tropical Animal and Plant Ecology of Hainan Province, College of Life Sciences, Hainan Normal University, Haikou, China; Guizhou Key Laboratory of Agricultural Microbiology, Guizhou Academy of Sciences, Guiyang, China
Guizhou Institute of Biology, Guizhou Academy of Sciences, 1 Longjiang Lane, Guiyang, China
Guizhou Institute of Biology, Guizhou Academy of Sciences, 1 Longjiang Lane, Guiyang, China; Guizhou Key Laboratory of Agricultural Microbiology, Guizhou Academy of Sciences, Guiyang, China
Guizhou Institute of Biology, Guizhou Academy of Sciences, 1 Longjiang Lane, Guiyang, China; Guizhou Key Laboratory of Agricultural Microbiology, Guizhou Academy of Sciences, Guiyang, China
Taxonomy Ascomata ITS Phylogeny Fungi Truffle

Abstract

Based on morphological and molecular phylogenetic evidence, a novel truffle species, Tuber guizhouense, is described from Populus-associated forests in Guizhou Province, China. This species is characterized by subglobose to globose ascomata (24–49 mm in diameter) with dark brown surfaces adorned by low pyramidal warts and occasional cracks. The mature gleba exhibits a marbled pattern with whitish veins. Ascospores are long-ellipsoid to cylindrical, bearing free spines (3–12 µm in height), and asci contain 1- spores. The combined phylogenetic analysis of ITS, 28S, and TEF1-α regions robustly support its distinctiveness (BS/PP = 100/1), placing it in a sister clade to the Japanese endemic T. longispinosum. Morphologically, T. guizhouense differs from related species (e.g., T. yigongense and T. sinense) by its pseudoparenchymatous peridium, unique spore ornamentation, and dark brown ascomata. This species represents the first wild truffle resource reported in Guizhou, occurring at altitudes of 1400–1500 m in symbiotic association with Populus trees, and is confirmed to be edibility. Its discovery expands the known subtropical distribution of Tuber and provides critical insights into the biodiversity, biogeography, and sustainable utilization of truffle resources in China.

References

  1. Airy-Shaw, H.K. (1931) Allia praesertim Sinensia nova vel minus cognita. Notes from the Royal Botanic Garden, Edinburgh 16: 134–147.
  2. Ashokan, A., Xavier, A., Suksathan, P., Ardiyani, M., Leong-Škorničková, J., Newman, M., Kress, W.J. & Gowda, V. (2022) Himalayan orogeny and monsoon intensification explain species diversification in an endemic ginger (Hedychium: Zingiberaceae) from the Indo-Malayan Realm. Molecular Phylogenetics and Evolution 170: 107440. https://doi.org/10.1016/j.ympev.2022.107440
  3. Baker, J.G. (1874) Original article on the Alliums of India, China, and Japan. Br J.G. Baker, F.L.S. The Journal of Botany, British and Foreign 12: 292–293.
  4. Burland, T.G. (2000) DNASTAR’s Lasergene sequence analysis software. Methods in Molecular Biology (Clifton, N.J.) 132: 71–91. https://doi.org/10.1385/1-59259-192-2:71
  5. Cheng, R.Y., Li, J., Xie, D.F., He, X.J., Zhou, R.X., Li, Q., Yu, Y. & Zhou, S.D. (2025) Effects of Mountain Uplift and Climatic Oscillations on Phylogeography and Species Divergence of Notholirion (Liliaceae). Journal of Heredity esaf032. https://doi.org/10.1093/jhered/esaf032
  6. Cheng, X.J., Fritsch, P.W., Lin, Y.J., Li, G.H., Chen, Y.Q., Zhang, M.Y. & Lu, L. (2024) The role of Pleistocene dispersal in shaping species richness of sky island wintergreens from the Himalaya-Hengduan Mountains. Molecular Phylogenetics and Evolution 197: 108082. https://doi.org/10.1016/j.ympev.2024.108082
  7. Darriba, D., Taboada, G.L., Doallo, R. & Posada, D. (2012) JModelTest 2: more models, new heuristics and parallel computing. Nature Methods 9: 772. https://doi.org/10.1038/nmeth.2109
  8. Friesen, N., Fritsch, R.M. & Blattner, F.R. (2006) Phylogeny and intrageneric classification of Allium (Alliaceae) based on nuclear ribosomal DNA ITS sequences. Aliso 22: 372–395. https://doi.org/10.5642/aliso.20062201.31
  9. Fritsch, R.M. & Abbasi, M. (2013) A taxonomic review of Allium subg. Melanocrommyum in Iran. Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung, Gatersleben, 240 pp.
  10. Fritsch, R.M. & Friesen, N. (2002) Evolution, domestication and taxonomy. In: Rabinowitch, H.D. & Currah, L. (Eds.) Allium crop science: recent advances. CABI Publishing, Wallingford, UK, pp. 5–30.
  11. Gao, Y.D., Harris, A.J. & He, X.J. (2015) Morphological and ecological divergence of Lilium and Nomocharis within the Hengduan Mountains and Qinghai-Tibetan Plateau may result from habitat specialization and hybridization. BMC Evolutionary Biology 15: 147. https://doi.org/10.1186/s12862-015-0405-2
  12. Govaerts, R., Kington, S., Friesen, N., Fritsch, R., Snijman, D.A., Marcucci, R., Silverstone-Sopkin, P.A. & Brullo, S. (2021) World checklist of Amaryllidaceae. Facilitated by the Royal Botanic Gardens, Kew. Available from: http://apps.kew.org/wcsp/ (accessed 28 August 2024)
  13. Huang, D.Q., Zhen, A.G. & Zhu, X.X. (2021) Allium yingshanense, a new species from the Dabie Mountains (East-central China), and taxonomic remarks on the related species. Phytotaxa 498 (4): 227–241. https://doi.org/10.11646/phytotaxa.498.4.1
  14. Huang, D.Q., Sun, H. & Ma, X.G. (2022) Allium jichouense (Amaryllidaceae), a new species of the section Sikkimensia from southwestern China. Phytotaxa 575: 115–128. https://doi.org/10.11646/phytotaxa.575.2.1
  15. Huang, D.Q., Sun, H. & Ma, X.G. (2024) Phylogenomic analyses and chromosome ploidy identification reveal multiple cryptic species in Allium sikkimense complex (Amaryllidaceae). Frontiers in Plant Science 14: 1268546. https://doi.org/10.3389/fpls.2023.1268546
  16. Kamelin, R.V. (1973) Florogeneticheskij analiz estestvennoj flory gornoj Srednej Azii. Leningrad, 354 pp.
  17. Katoh, K. & Standley, D.M. (2013) MAFFT multiple sequence alignment software version 7.0: improvements in performance and usability. Molecular Biology and Evolution 30: 772–780. https://doi.org/10.1093/molbev/mst010
  18. Kumar, S., Stecher, G. & Tamura, K. (2016) Mega7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular Biology and Evolution 33: 1870–1874. https://doi.org/10.1093/molbev/msw054
  19. Li, Q.Q., Zhou, S.D., He, X.J., Yu, Y., Zhang, Y.C. & Wei, X.Q. (2010) Phylogeny and biogeography of Allium (Amaryllidaceae: Allieae) based on nuclear ribosomal internal transcribed spacer and chloroplast rps16 sequences, focusing on the inclusion of species endemic to China. Annals of Botany 106: 709–733. https://doi.org/10.1093/aob/mcq177
  20. Linnaeus, C. (1753) Species Plantarum, vol. 1. Impensis Laurentius Salvius, Holmiae, 560 pp.
  21. McLay, T.G.B., Fowler, R.M., Fahey, P.S., Murphy, D.J., Udovicic, F., Cantrill, D.J. & Bayly, M.J. (2023) Phylogenomics reveals extreme gene tree discordance in a lineage of dominant trees: hybridization, introgression, and incomplete lineage sorting blur deep evolutionary relationships despite clear species groupings in Eucalyptus subgenus Eudesmia. Molecular phylogenetics and evolution 187: 107869. https://doi.org/10.1016/j.ympev.2023.107869
  22. Meng, H.H., Su, T., Gao, X.Y., Li, J., Jiang, X.L., Sun, H. & Zhou, Z.K. (2017) Warm-cold colonization: response of oaks to uplift of the Himalaya-Hengduan Mountains. Molecular Ecology 26 (12): 3276–3294. https://doi.org/10.1111/mec.14092
  23. Nguyen, N.H., Driscoll, H.E. & Specht, C.D. (2008) A molecular phylogeny of the wild onions (Allium: Alliaceae) with a focus on the western North American center of diversity. Molecular Phylogenetics and Evolution 47: 1157–1172. https://doi.org/10.1016/j.ympev.2007.12.006
  24. Pandey, A., Pandey, R., Negi, K.S. & Radhamani, J. (2008) Realizing value of genetic resources of Allium in India. Genetic Resources and Crop Evolution 55: 985–994. https://doi.org/10.1007/s10722-008-9305-2
  25. Regel, E. (1875) Alliorum adhuc cognitorum monographia. Trudy Imperatorskago S.-Peterburgskago Botaničeskago Sada. 3 (2): 1–266. https://doi.org/10.5962/bhl.title.15473
  26. Rendle, A.B. (1906) New monocotyledons from China and Tibet. Journal of Botany, British and Foreign. London 44: 41–46.
  27. Ronquist, F. & Huelsenbeck, J.P. (2003) MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19: 1572–1574. https://doi.org/10.1093/bioinformatics/btg180
  28. Shaw, J., Lickey, E.B., Beck, J.T., Farmer, S.B., Liu, W.S., Miller, J., Siripun, K.C., Winder, C.T., Schilling, E.E. & Small, R.L. (2005) The tortoise and the hare II: relative utility of 21 noncoding chloroplast DNA sequences for phylogenetic analysis. American Journal of Botany 92: 142–166. https://doi.org/10.3732/ajb.92.1.142
  29. Shaw, J., Lickey, E., Schilling, E. & Small, R. (2007) Comparison of whole chloroplast genome sequences to choose noncoding regions for phylogenetic studies in angiosperms: the tortoise and the hare III. American Journal of Botany 94: 275–288. https://doi.org/10.3732/ajb.94.3.275
  30. Smith, W.W. (1914) Allium beesianum. Notes from the Royal Botanic Garden, Edinburgh. Edinburgh and Glasgow 8: 176–177.
  31. Stamatakis, A. (2014) RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 30: 1312–1313. https://doi.org/10.1093/bioinformatics/btu033
  32. Swofford, D.L. (2003) PAUP*: Phylogenetic analysis using parsimony (*and other methods), version 4.0b10. Sunderland: Sinauer Associates.
  33. Traub, H.P. (1972) Genus Allium L. subgenera, sections and subsections. Plant Life 28: 132–137.
  34. Wang, F.T. & Tang, T. (1937) Notes on Chinese Liliaceae IV. Bulletin of the Fan Memorial Institute of Biology; Botany. Beijing 7: 281–304.
  35. Wang, K.L., Zhou, X.H., Liu, D.T., Li, Y.Z., Yao, Z., He, W.M. & Liu, Y.B. (2022) The uplift of the Hengduan Mountains contributed to the speciation of three Rhododendron species. Global Ecology and Conservation 35: e02085. https://doi.org/10.1016/j.gecco.2022.e02085
  36. White, T.J., Bruns, T., Lee, S. & Taylor, J. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis, M.A., Gelfand, D.H., Sninsky, J.J. & White, T.J. (Eds.) PCR Protocols: A Guide to Methods and Applications. Academic Press Inc, San Diego, California, pp. 315–322. https://doi.org/10.1016/B978-0-12-372180-8.50042-1
  37. Xia, M.Q., Liu, Y., Liu, J.J., Chen, D.H., Shi, Y., Chen, Z.X., Chen, D.R., Jin, R.F., Chen, H.L., Zhu, S.S., Li, P., Si, J.P. & Qiu, Y.X. (2022) Out of the Himalaya-Hengduan Mountains: Phylogenomics, biogeography and diversification of Polygonatum Mill. (Asparagaceae) in the Northern Hemisphere. Molecular Phylogenetics and Evolution 169: 107431. https://doi.org/10.1016/j.ympev.2022.107431
  38. Xie, C., Xie, D.F., Zhong, Y., Guo, X.L., Liu, Q., Zhou, S.D. & He, X.J. (2019) The effect of Hengduan Mountains Region (HMR) uplift to environmental changes in the HMR and its eastern adjacent area: tracing the evolutionary history of Allium section Sikkimensia (Amaryllidaceae). Molecular Phylogenetics and Evolution 130: 380–396. https://doi.org/10.1016/j.ympev.2018.09.011
  39. Xie, D.F., Cheng, R.Y., Megan, Chen, J.P., Lei, J.Q., Zhang, X.Y. & He, X.J. (2022) Allium heterophyllum (Amaryllidaceae), a new species from Henan, China. PhytoKeys 190: 53–67. https://doi.org/10.3897/phytokeys.190.77449
  40. Xie, D.F., Cheng, R.Y., Deng, J.J., Wang, Y. & He, X.J. (2024) Allium kangdingense (Amaryllidaceae, Allioideae), a new species of section Sikkimensia from western Sichuan. Phytotaxa 638 (2): 175–184. https://doi.org/10.11646/phytotaxa.638.2.6
  41. Xu, J.M. (1980) Allium L. In: Wang, F.T. & Tang, T. (Eds.) Flora Reipublicae Popularis Sinicae (Monocotyledoneae-Liliaceae). Science press, Beijing, pp. 170–272, 283–286.
  42. Zhang, Z.Z., Liu, G. & Li, M.J. (2024) Incomplete lineage sorting and gene flow within Allium (Amayllidaceae). Molecular phylogenetics and evolution 195: 108054. https://doi.org/10.1016/j.ympev.2024.108054

How to Cite

Wang, J., Yang, Y.H., Xiang, Z. & Deng, C.Y. (2025) Tuber guizhouense sp. nov. (Tuberaceae, Ascomycota) from Guizhou Province, China. Phytotaxa 712 (1): 59–66. https://doi.org/10.11646/phytotaxa.712.1.5