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Type: Article
Published: 2023-07-18
Page range: 150-162
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A new species of Saxifraga (Saxifragaceae) from northeastern Anatolia, Turkey

Pamukkale University, Faculty of Science, Department of Biology, 20160, Kinikli, Denizli, Turkey
Pamukkale University, Faculty of Science, Department of Biology, 20160, Kinikli, Denizli, Turkey
Muğla Sıtkı Koçman University, Faculty of Science, Molecular Biology and Genetic Department, 48000, Kötekli, Muğla, Turkey
endemic ISSR Rize Saxifraga kavrunensis taxonomy Eudicots

Abstract

Saxifraga kavrunensis, a new species endemic to Turkey, is described and illustrated. Samples from its natural population were collected from the Kaçkar Mountains in Rize Province. Although it has similar features to S. paniculata subsp. paniculata, it differs in its longer multi-flowered stem and maroon-spotted corolla. In this study, the diagnostic characteristics of S. kavrunensis were elucidated at the morphological, micromorphological and molecular level. In the molecular-based phylogenetic analyses, S. kavrunensis was compared with S. paniculata subsp. paniculata, to which it bears the closest morphological resemblance. After PCR analyses, reproductive band profiles were obtained from three of the eighteen ISSR primers tested. The 66 polymorphic band profiles, ranging from 230 bp to 4000 bp, were obtained from the agarose gel electrophoresis images of these three primers. According to a distance matrix based on the Jaccard coefficient, significant variation was detected at the species level, with indices varying between 0.395 and 0.614 between both species.

References

  1. Aslan, S. (2012) Saxifraga L. In: Güner, A., Aslan, S., Ekim, T., Vural, M. & Babaç, M.T. (Eds.) Türkiye Bitkileri Listesi (Damarlı Bitkiler). Nezahat Gökyiğit Botanik Bahçesi ve Flora Araştırmaları Derneği Yayını, Istanbul, pp. 843–845. [in Turkish]
    Backlund, M. & Thulin, M. (2007) Revision of the Mediterranean species of Plocama (Rubiaceae). Taxon 56 (2): 516–520. https://doi.org/10.1002/tax.562022
    Blaxland, K. (2004) A new species of Viola (Violaceae) from south-west Turkey. Botanical Journal of the Linnean Society 145 (4): 505–509. https://doi.org/10.1111/j.1095-8339.2004.00294.x
    Boissier, E. & Heldreich, T.H. (1849) Diagnoses Plantarum Orientalium Novarum 8: 56.
    Dirks-Mulder, A., Ahmed, I., uit het Broek, M., Krol, L., Menger, N., Snier, J., van Winzum, A., de Wolf, A., van’t Wout, M., Zeegers, J.J., Butôt, R., Heijungs, R., van Heuven, B.J., Kruizinga, J., Langelaan, R., Smets, E.F., Star, W., Bemer, M. & Gravendeel, B. (2019) Morphological and Molecular Characterization of Orchid Fruit Development. Frontiers in Plant Science 10: 137. https://doi.org/10.3389/fpls.2019.00137
    Doyle, J.J. & Doyle, J.L. (1987) A Rapid DNA Isolation Procedure for Small Quantities of Fresh Leaf Tissue. Phytochemical Bulletin 19, 11–15.
    Düşen, O., Göktürk, R.S., Kaya, E., Sarpkaya, U. & Gürcan, B. (2018) Morphological and molecular determination of a new Viola species (Violaceae) from Turkey. Phytotaxa 369 (1): 37–46. https://doi.org/10.11646/phytotaxa.369.1.4
    El-Assal, S.E.D. & Gaber, A. (2012) Discrimination capacity of RAPD, ISSR and SSR markers and of their effectiveness in establishing genetic relationship and diversity among Egyptian and Saudi wheat cultivars. American Journal of Applied Sciences 9 (5): 724–735. https://doi.org/10.3844/ajassp.2012.724.735
    Erdtman, G. (1969) Handbook of palynology: morphology, taxonomy, ecology. An introduction to the study of pollen grains and spores. Munksgaard, Copenhagen. 486 pp.
    Feder, J.L., Egan, S.P. & Nosil, P. (2012) The genomics of speciation-with-gene-flow. Trends in Genetics 28 (7): 342–350. https://doi.org/10.1016/j.tig.2012.03.009
    Felix, F.C., das Chagas, K.P.T., dos Santos Ferrari, C., de Almeida Vieira, F. & Pacheco, M.V. (2020) Applications of ISSR markers in studies of genetic diversity of Pityrocarpa moniliformis. Revista Caatinga 33 (4): 1017–1024. https://doi.org/10.1590/1983-21252020v33n417rc
    Ferrand, E., Dumat, C., Leclerc-Cessac, E. & Benedetti, M.F. (2006) Phytoavailability of zirconium in relation to its initial added form and soil characteristics. Plant and Soil 287: 313–325. https://doi.org/10.1007/s11104-006-9079-2
    Fırat, M. (2016) Saxifraga hakkariensis (Saxifragaceae), a new species from Hakkari province (Turkey) belonging to section Porphyrion. Phytotaxa 289 (2): 181–187. https://doi.org/10.11646/phytotaxa.289.2.8
    Garrido-Cardenas, J.A., Mesa-Valle, C. & Manzano-Agugliaro, F. (2018) Trends in plant research using molecular markers. Planta 247: 543–557. https://doi.org/10.1007/s00425-017-2829-y
    Göktürk, R.S., Düşen, O., Kaya, E., Gürcan, B. & Sarpkaya, U. (2019) A new variety of Plocama calabrica (Rubiaceae) from Denizli (Turkey) confirmed by morphological and molecular ISSR markers. Acta Botanica Croatica 78 (2): 142–146. https://doi.org/10.2478/botcro-2019-0016
    Göktürk, R.S., Yüceol, F., Galatalı, S., Kaya, E., Hamzaoğlu, E. & Koç, M. (2023) A new Cephalaria (Caprifoliaceae) species from Turkey. Phytotaxa 587 (2): 173–183. https://doi.org/10.11646/phytotaxa.587.2.6
    Grover, A. & Sharma, P.C. (2016) Development and use of molecular markers: past and present. Critical Reviews in Biotechnology 36 (2): 290–302. https://doi.org/10.3109/07388551.2014.959891
    Gupta, M, Chyi, Y.S., Romero-Severson, J. & Owen, J.L. (1994) Amplification of DNA markers from evolutionarily diverse genomes using single primers of simple sequence repeats. Theoretical Applied Genetics 89: 998–1006. https://doi.org/10.1007/BF00224530
    Halbritter, H., Ulrich, S., Grímsson, F., Weber, M., Zetter, R., Hesse, M., Buchner, R., Svojtka, M. & Frosch-Radivo, A. (2018) Illustrated Pollen Terminology. Springer, Cham, Switzerland, 483 pp. https://doi.org/10.1007/978-3-319-71365-6
    Hasan, N., Choudhary, S., Naaz, N., Sharma, N. & Laskar, R.A. (2021) Recent advancements in molecular marker-assisted selection and applications in plant breeding programmes. Journal of Genetic Engineering and Biotechnology 19: 128. https://doi.org/10.1186/s43141-021-00231-1
    Haynes, W.M. (2017) Abundance of elements in the earth’s crust and in the sea. In: Haynes, W.M (Ed.) CRC Handbook of Chemistry and Physics, 97th edition. CRC Press Taylor & Francis Group, Boca Raton, FL, Section 14, pp. 17–18.
    Hodson, M. (2002) Experimental evidence for mobility of Zr and other trace elements in soils. Geochimica et Cosmochimica Acta 66: 819–828. https://doi.org/10.1016/S0016-7037(01)00803-1
    Hu, J. & Vick, B.A. (2003) Target region amplification polymorphism: a novel marker technique for plant genotyping. Plant Molecular Biology Reporter 21: 289–294. https://doi.org/10.1007/BF02772804
    IUCN (2012) IUCN Red List Categories and Criteria, version 3.1, 2. ed. Gland, Switzerland and Cambridge (UK), 32 pp.
    IPNI (2015) The International Plant Names Index. Available from: http://www.ipni.org (Retrieved June 23, 2018).
    Jussieu, A.L. de (1789) Genera Plantarum. Herissant Barrois, Paris, 498 pp.
    Kabata-Pendias, A. & Pendias, H. (1992) Trace elements in soils and plants, CRC Press Taylor & Francis Group, Boca Raton, FL, 365 pp.
    Kaplan, K. (1981) Embryologische, pollen- und samenmorphologische Untersuchungen zur Systematik von Saxifraga (Saxifragaceae). Bibliotheca Botanica 134: 1–56.
    Kaya, E. & Yilmaz Gokdogan, E. (2016) Using two retrotransposon based marker systems (IRAP and REMAP) for Molecular Characterization of olive (Olea europaea L.) cultivars. Notulae Botanicae Horti Agrobotanici Cluj-Napoca 44 (1): 167–174. https://doi.org/10.15835/nbha44110158
    Kaya, E. (2015) ISSR analysis for determination of genetic diversity and relationship in eight Turkish olive (Olea europaea L.) cultivars. Notulae Botanicae Horti Agrobotanici Cluj-Napoca 43 (1): 96–99. https://doi.org/10.15835/nbha4319818
    Kaya, E. & Souza, F.V.D. (2017) Comparison of two PVS2-based procedures for cryopreservation of commercial sugarcane (Saccharum spp.) germplasm and confirmation of genetic stability after cryopreservation using ISSR markers. In Vitro Cellular & Developmental Biology-Plant 53: 410–417. https://doi.org/10.1007/s11627-017-9837-2
    Kiester, A.R., Lande, R. & Schemske, D.W. (1984) Models of co-evolution and speciation in plants and their pollinators. The American Naturalist 124: 220–243. https://doi.org/10.1086/284265
    Kumar, P., Gupta, V., Misra, A., Modi, D. & Pandey, B. (2009) Potential of molecular markers in plant biotechnology. Plant Omics 2 (4): 141–162.
    Levin, D.A. (1993) Local speciation in plants: The rule not the exception. Systematic Botany 18 (2): 197–208. https://doi.org/10.2307/2419397
    Linnaeus, C. (1753) Species Plantarum. Laurentii Salvii, Stockholm, 1200 pp.
    Macnair, M.R. (1989) The potential for rapid speciation in plants. Genome 31 (1): 203–210. https://doi.org/10.1139/g89-035
    Marchese, C. (2015) Biodiversity hotspots: A shortcut for a more complicated concept. Global Ecology and Conservation 3: 297–309. https://doi.org/10.1016/j.gecco.2014.12.008
    Matthews, V.A. (1972) Saxifraga L. and Chrysosplenium L. In: Davis, P.H. (Ed.) Flora of Turkey and the East Aegean Islands, vol. 4. Edinburgh University Press, Edinburgh, pp. 249–260.
    Miller, P. (1768) Gardeners Dictionary, ed. 8. J. & F. Rivington, London, 1366 pp.
    Mirzaei, S. (2021) Application of molecular markers in plant sciences; An overview. Central Asian Journal of Plant Science Innovation 1 (4): 192–200. https://doi.org/10.22034/CAJPSI.2021.04.02
    Nasar, S., Ostevik, K., Murtaza, G. & Rausher, M.D. (2022) Morphological and molecular characterization of variation in common bean (Phaseolus vulgaris L.) germplasm from Azad Jammu and Kashmir, Pakistan. PloS ONE 17 (4): e0265817. https://doi.org/10.1371/journal.pone.0265817
    Nei, M. (1978) Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 89 (3): 583–590. https://doi.org/10.1093/genetics/89.3.583
    Nei, M. & Nozawa, M. (2011) Roles of mutation and selection in speciation: From Hugo de Vries to the Modern Genomic Era. Genome Biology and Evolution 3: 812–829. https://doi.org/10.1093/gbe/evr028
    Ozudogru, E.A., Kaya, E., Kirdok, E. & Issever-Ozturk, S. (2011) In vitro propagation from young and mature explants of thyme (Thymus vulgaris and T. longicaulis) resulting in genetically stable shoots. In Vitro Cellular & Developmental Biology-Plant 47: 309–320. https://doi.org/10.1126/science.1129647
    Pagel, M., Venditti, C. & Meade, A. (2006) Large punctuational contribution of speciation to evolutionary divergence at the molecular level. Science 314: 119–121. https://doi.org/10.1007/s11627-011-9347-6
    Postel, Z. & Touzet, P. (2020) Cytonuclear genetic incompatibilities in plant speciation. Plants 9: 487. https://doi.org/10.3390/plants9040487
    Roemer, J.J. & Schultes, J.A. (1819) Systema Vegetabilium: secundum classes, ordines, genera, species. Cum characteribus, differentiis et synonymiis, 15, vol. 5. Sumtibus J.G. Cottae, Stuttgardtiae, pp. 383. https://doi.org/10.5962/bhl.title.825
    Sarma, B. & Tanti, B. (2017) Analysis of genetic diversity of certain species of Aristolochia using ISSR-based molecular markers. Current Life Sciences 3 (4): 47–53. http://dx.doi.org/10.5281/zenodo.883637
    Shahid, M., Ferrand, E., Schreck, E. & Dumat, C. (2013) Behavior and Impact of Zirconium in the Soil–Plant System: Plant Uptake and Phytotoxicity. In: Whitacre, D. (Ed.) Reviews of Environmental Contamination and Toxicology, vol. 221. Springer, New York. pp. 107–127. https://doi.org/10.1007/978-1-4614-4448-0_2
    Shi, J.J. & Chen, H. (2002) Dynamics of accumulation and disappearance of zirconium-95 in the maize/soil and soybean/soil ecosystem. Journal of Environmental Sciences 23: 109–113.
    Shi, J.J. & Guo, J.F. (2002) Uptake from soil and distribution of 95Zr in Chinese cabbage. The Journal of Agricultural Science 139: 431–435. https://doi.org/10.1017/S0021859602002721
    Shi, J.J., Guo, J.F. & Chen, H. (2002) Dynamics of 95Zr in the rice/water/soil system. Applied Radiation and Isotopes 56: 735–740. https://doi.org/10.1016/S0969-8043(01)00257-3
    Solomon, J., Shulkina, T. & Schatz, G.E. (2013) Red List of the endemic plants of the Caucasus: Armenia, Azerbaijan, Georgia, Iran, Russia and Turkey. Missouri Botanical Garden Press, Saint Louis, 451 pp.
    Soltis, D.E. (2007) Compositae. In: Kubitzki, K. (Ed.) The families and genera of vascular plants 9. Springer, Berlin, pp. 418–435. https://doi.org/10.1007/978-3-540-32219-1_47
    Strasburg, J.L., Sherman, N.A., Wright, K.M., Moyle, L.C., Willis, J.H. & Rieseberg, L.H. (2012) What can patterns of differentiation across plant genomes tell us about adaptation and speciation? Philosophical Transactions of the Royal Society B 367: 364–373. https://doi.org/10.1098/rstb.2011.0199
    Terzioğlu, S., Coşkunçelebi, K. & Güzel, M.E. (2019) A new subspecies of Saxifraga artvinensis V.A.Matthews (Saxifragaceae) from northeastern Anatolia. Turkish Journal of Botany 43: 687–693. https://doi.org/10.3906/bot-1901-19
    Tkach, N., Röser, M., Miehe, G., Muellner-Riehl, A.N., Ebersbach, J., Favre, A. & Hoffmann, M.H. (2015) Molecular phylogenetics, morphology and a revised classification of the complex genus Saxifraga (Saxifragaceae). Taxon 64: 1159–1187. https://doi.org/10.12705/646.4
    Üstüner, H., Yavuz, M., Göktürk, R.S., Kaya, E. & Galatalı, S. (2022) Morphological and molecular notes of Gypsophila pilulifera. Phytotaxa 556 (2): 99–118. https://doi.org/10.11646/phytotaxa.556.2.1
    Vimala, Y., Lavania, S. & Lavania, U.C. (2021) Chromosome change and karyotype differentiation–implications in speciation and plant systematics. The Nucleus 64: 33–54. https://doi.org/10.1007/s13237-020-00343-y
    Webb, D.A. (1964) Saxifragaceae. In: Tutin, T.G., Heywood, V.H., Burges, N.A., Valentine, D.H., Walters, S.M., Webb, D.A. Flora Europaea, vol. 1. Cambridge University Press, Cambridge, pp. 437–458.
    Wakabayashi, M. (1973) On Saxifraga sect. Diptera of Japan, with description of a new species. Acta Phytotaxonomica et Geobotanica 25: 154–169.
    Wodehouse, R.P. (1935) Pollen Grains. Mc Graw Press, New York, pp. 75.
    Yuan, C.Y., Wang, P., Chen, P.P., Xiao, W.J., Zhang, C., Hu, S., Zhou, P., Chang, H.P., He, Z., Hu, R., Lu, X.T., Ye, J.Z. & Guo, X.H. (2015) Genetic diversity revealed by morphological traits and ISSR markers in 48 Okras (Abelmoschus escullentus L.). Physiology and Molecular Biology of Plants 21 (3): 359–364. https://doi.org/10.1007/s12298-015-0303-5
    Zhang, Z. (2013) Phylogenetic studies in the genus Saxifraga (Saxifragaceae). Department of Biology, University of Leicester, UK, 324 pp.
    Zhang, Z., Chen, S. & Gornall, R.J. (2015) Morphology and anatomy of the exine in Saxifraga (Saxifragaceae). Phytotaxa 212 (2): 105–132. https://doi.org/10.11646/phytotaxa.212.2.1
    Zietkiewicz, E., Rafalski, A. & Labuda, D. (1994) Genome fingerprinting by simple sequence repeats (SSR)-anchored PCR amplification. Genomics 20: 176–183. https://doi.org/10.1006/geno.1994.1151