Lactarius verrucosporus sp. nov. and L. nigricans sp. nov., two new species of Lactarius (Russulaceae) from southern China

GENG-SHEN WANG; YU SONG; JING-WEI LI; SHI-YAO XIA; LI-HONG QIU

doi:10.11646/phytotaxa.364.3.2

Issue: Vol. 364 No. 3: 14 August 2018

Type: Article

Published: 2018-08-14

DOI: 10.11646/phytotaxa.364.3.2

Page range: 227–240

Abstract views: 25

PDF downloaded: 1

Lactarius verrucosporus **sp. nov. and L. nigricans sp. nov., two new species of Lactarius (Russulaceae) from southern China**

GENG-SHEN WANG⁺⁻
YU SONG⁺⁻
JING-WEI LI⁺⁻
SHI-YAO XIA⁺⁻
LI-HONG QIU⁺⁻

State Key Lab of Biocontrol, School of Life Science, Sun Yat-sen University, Guangzhou 510275, China

Fungi Lactarius new taxa phylogeny taxonomy

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Abstract

Two new species of Lactarius from Dinghu Mountain, Guangdong Province in southern China were described based on both morphological characters and phylogenetic analysis of internal transcribed spacer (ITS) sequences. Lactarius verrucosporus sp. nov. is characterized by reddish brown pileus with white and decurved margin, pale cream lamellae, globose to subglobose basidiospores with isolated warts, thin pileipellis composed of globose subterminal cells and inflated terminal elements. Lactarius nigricans sp. nov. is recognized by greyish brown pileus, beige lamellae, amyloid basidiospores with high ridges, subcylindrical to subfusiform caulocystidia, thick pileipellis with long terminal cells. Both molecular and morphological analysis consistently confirm that L. verrucosporus and L. nigricans belong to Lactarius subgenera Russularia and Plinthogalus, respectively, and both species are distinct from all described species of these two subgenera. The morphological differences of these two novel species with their closely related taxa are discussed.

References

Bandala, V. M., Montoya, L. & Ramos, A. (2016) Two new Lactarius species from a subtropical cloud forest in eastern Mexico. Mycologia 108 (5): 967–980. https://doi.org/10.3852/15-310Barge, E.G. & Cripps, C.L. (2016) New reports, phylogenetic analysis, and a key to Lactarius Pers. in the Greater Yellowstone Ecosystem informed by molecular data. MycoKeys 15: 1. https://doi.org/10.3897/mycokeys.15.9587Barge, E.G., Cripps, C.L. & Osmundson, T.W. (2016) Systematics of the ectomycorrhizal genus Lactarius in the Rocky Mountain alpine zone. Mycologia 108 (2): 414–440. https://doi.org/10.3852/15-177Beenken, L., Sainge, M.N. & Kocyan, A. (2016) Lactarius megalopterus, a new angiocarpous species from a tropical rainforest in Central Africa, shows adaptations to endozoochorous spore dispersal. Mycological progress 15 (6): 58. https://doi.org/10.1007/s11557-016-1198-4Bi, Z.S., Zheng, G.Y. & Li, T.H. (1994) Macrofungus flora of Guangdong Province. Guangdong Science and Technology Press, Guangzhou, pp. 1–879.Buyck, B., Duhem, B., Das, K., Jayawardena, R.S., Niveiro, N., Pereira, O.L., Prasher, I.B., Adhikari, S., Albertó, E.O., Bulgakov, T.S., Castañeda-Ruiz, R.F., Hembrom, M.E., Hyde, K.D., Lewis, D.P., Michlig, A., Nuytinck, J., Parihar, A., Popoff, O.F., Ramirez, N.A., Da Silva, M., Verma, R.K. & Hofstetter, V. (2017) Fungal Biodiversity Profiles. Cryptogamie, Mycologie: 21–30. https://doi.org/10.7872/crym/v38.iss1.2017.101Buyck, B., Hofstetter, V., Eberhardt, U., Verbeken, A. & Kauff, F. (2008) Walking the thin line between Russula and Lactarius: the dilemma of Russula subsect. Ochricompactae. Fungal Diversity 28: 15–40.Buyck, B., Hofstetter, V., Verbeken, A. & Walleyn, R. (2010) Proposal to conserve Lactarius nom. cons. (Basidiomycota) with a conserved type. Taxon 59: 295–296.Das, K. & Chakraborty, D. (2015) Lactarius vesterholtii, a new species from India. Mycotaxon 129 (2): 477–484. https://doi.org/10.5248/129.477Das, K. & Verbeken, A. (2012) New Species of Lactarius Subg. Plinthogalus and New Records of Lactifluus Subg. Gerardii (Russulaceae) from Sikkim, India. Taiwania 57 (1): 37–48. Das, K., Ghosh, A., Li, J.W., Qiu, L.H., Baghela, A., Halama, M., Hembrom, M.E., Mehmood, T., Parihar, A., Pencakowski, B., Bielecka, M., Reczynska, K., Sasiela, D., Singh, U., Song, Y., Świerkosz, K., Szczesniak, K., Uniyal, P., Zhang, J.B. & Buyck, B. (2017) Fungal Biodiversity Profiles 31–40. Cryptogamie Mycologie 38 (3): 1–54. https://doi.org/10.7872/crym/v38.iss3.2017.353Das, K., Sharma, J.R., Basso, M.T. & Bhatt, R.P. (2005) Lactarius in Kumaon Himalaya 4: A new species of subgenus Piperites. Mycotaxon 91: 1–7.Das, K., Verbeken, A. & Nuytinck, J. (2015) Morphology and phylogeny of four new Lactarius species from Himalayan India. Mycotaxon 130 (1): 105–130. https://doi.org/10.5248/130.105Das, K., Verbeken, A., Chakraborty, D., Avchar, R. & Baghela, A. (2018) Morphological and Phylogenetic Evidence for Two New Lactarius Species (Russulales, Basidiomycota) from India. Cryptogamie, Mycologie.Drummond, A.J. & Rambaut, A. (2007) Beast: Bayesian evolutionary analysis by sampling trees. BMC Evolutionary Biology 7: 214. https://doi.org/10.1186/1471-2148-7-214Eberhardt, U. (2000) Moleculare Analysen zur Verwandtschaft der agaricoiden Russulaceen im Vergleich mit Mykorrhiza-und Fruchtkörpermerkmalen. PhD dissertation, University of Tübingen, Germany.Heilmann-Clausen, J. (1998) The genus Lactarius. Fungi of northern Europe.Kanad, D. & Annemieke, V. (2011) Three new species of Lactarius (Russulaceae) from Sikkim, India. Cryptogamie, Mycologie 32 (4): 365–381. https://doi.org/10.7872/crym.v32.iss4.2011.365Kuo, M., Methven, A.S., Minnis, A.M. & Halling, R.E. (2013) Studies of North American macrofungi, 1. Validation of Lactarius rubidus comb. nov. and Leccinellum quercophilum sp. nov. Mycotaxon 124 (1): 323–332. https://doi.org/10.5248/124.323Latha, K.D., Raj, K.A., Farook, V.A., Sharafudheen, S.A., Parambil, N.K. & Manimohan, P. (2016) Three new species of Russulaceae from India based on morphology and molecular phylogeny. Phytotaxa 246 (1): 61–77. https://doi.org/10.11646/phytotaxa.246.1.5Le, H.T., Nuytinck, J., Verbeken, A., Lumyong, S. & Desjardin, D.E. (2007) Lactarius in Northern Thailand: 1. Lactarius subgenus Piperites. Fungal Diversity 24, 173–224.Le, H.T., Stubbe, D., Verbeken, A., Nuytinck, J., Lumyong, S. & Desjardin, D.E. (2007) Lactarius in Northern Thailand: 2. Lactarius subgenus Plinthogali. Fungal Diversity 27 (1): 61–94.Lee, H., Park, M.S., Jung, P.E., Fong, J.J., Oh, S.Y., Verbeken, A. & Lim, Y.W. (2015) Lactarius cucurbitoides (Russulales, Basidiomycota), a new species from South Korea supported by molecular and morphological data. Phytotaxa 205 (3): 168–176. https://doi.org/10.11646/phytotaxa.205.3.4Li, G.J. Hyde, K.D., Zhao, R.L., Hongsanan, S., Abdel-Aziz, F.A., Abdel-Wahab, M.A., Alvarado, P., Alves-Silva, G., Ammirati, J.F., Ariyawansa, H.A., Ali, A.B., Bahkali, H., Beug, M., Bhat, D.J., Bojantchev, D., Boonpratuang, T., Bulgakov, T.S., Camporesi, E., Boro, M.C., Ceska, O., Chakraborty, D., Chen, J.J., Chethana, K.W.T., Chomnunti, P., Consiglio, G., Cui, B.K., Dai, D.Q., Dai, Y.C., Daranagama, D.A., Das, K., Dayarathne, M.C., De Crop, E., De Oliveira, R.J.V., de Souza, C.A.F., de Souza, J.I., Dentinger, B.T.M., Dissanayake. A.J., Doilom, M., Drechsler-Santos, E.R., Ghobad-Nejhad, M., Gilmore, M.P., Góes-Neto, A., Gorczak, M., Haitjema, C.H., Hapuarachchi, K.K., Hashimoto, A., He, M.Q., Henske, J.K., Hirayama, K., Iribarren, M.J., Jayasiri, S.C., Jayawardena, R.S., Jeon, S.J., Jerônimo, G.H., Jesus, A.L., Jones, E.B.G., Kang, J.C., Karunarathna, S.C., Kirk, P.M., Konta, S., Kuhnert, E., Langer, E., Lee, H.S., Lee, H.B., Li, W.J., Li, X.H., Liimatainen, K., Lima, D.X., Lin, C.G., Liu, J.K., Liu, X.Z., Liu, Z.Y., Luangsa-ard, J.J., Lücking, R., Lumbsch, H.T., Lumyong, S., Leaño, E.M., Marano, A.V., Matsumura, M., McKenzie, E.H.C., Mongkolsamrit, S., Mortimer, P.E., Nguyen, T.T.T., Niskanen, T., Norphanphoun, C., O’Malley, M.A., Parnmen, S., Pawłowska, J., Perera, R.H., Phookamsak, R., Phukhamsakda, C., Pires-Zottarelli, C.L.A., Raspé, O., Reck, M.A., Rocha, S.C.O., de Santiago, A.L.C.L.A., Senanayake, I.C., Setti, L., Shang, Q.J., Singh, S.K., Sir, E.B., Solomon, K.V., Song, J., Srikitikulchai, P., Stadler, M., Suetrong, S., Takahashi, H., Takahashi, T., Tanaka, K., Tang, L.P., Thambugala, K.M., Thanakitpipattana, D., Theodorou, M.K., Thongbai, B., Thummarukcharoen, T., Tian, Q., Tibpromma, S., Verbeken, A., Vizzini, A., Vlasák, J., Voigt, K., Wanasinghe, D.N., Wang, Y., Weerakoon, G., Wen, H.A., Wen, T.C., Wijayawardene, N.N., Wongkanoun, S., Wrzosek, M., Xiao, Y.P., Xu, J.C., Yan, J.Y., Yang, J., Yang, S.D., Hu, Y., Zhang, J.F., Zhao, J., Zhou, L.W., Peršoh, D., Phillips, A.J.L. & Maharachchikumbura, S.S.N. (2016) taxonomic and phylogenetic contributions to fungal taxa. Fungal Diversity 78 (1): 1–237. https://doi.org/10.1007/s13225-016-0366-9Liu, J.K., Hyde, K.D., Jones, E.B.G., Ariyawansa, H.A., Bhat, D.J., Boonmee, S., Maharachchikumbura, S.S.N., McKenzie, E.H.C., Phookamsak, R., Phukhamsakda, C., Shenoy, B.D., Abdel-Wahab, M.A., Chen, B.B., Chethana, K.W.T., Singtripop, C., Dai, D.Q., Dai, Y.C., Daranagama, D.A., Dissanayake, A.J., Doilom, M., D’souza, M.J., Fan, X.L., Goonasekara, I.D., Hirayama, K., Hongsanan, S., Jayasiri, S.C., Jayawardena, R.S., Karunarathna, S.C., Li, W.J., Mapook, A., Norphanphoun, C., Pang, K.L., Perera, R.H., Peršoh, D., Pinruan, U., Senanayake, I.C., Somrithipol, S., Suetrong, S., Tanaka, K., Thambugala, K.M., Tian, Q., Tibpromma, S., Udayanga, D., Wijayawardene, N.N., Wanasinghe, D., Wisitrassameewong, K., Zeng, X.Y., Abdel-Aziz, F.A., Adamčík, S., Bahkali, A.H., Boonyuen, N., Bulgakov, T., Callac, P., Chomnunti, P., Greiner, K., Hashimoto, A., Hofstetter, V., Kang, J.C, Lewis, D., Li, X.H., Liu, X.Z., Liu, Z.Y., Matsumura, M., Mortimer, P.E., Rambold, G., Randrianjohany, E., Sato, G., Sri-Indrasutdhi, V., Tian, C.M., Verbeken, A., von Brackel, W., Wang, Y., Wen, T.C., Xu, J.C., Yan, J.Y., Zhao, R.L. & Camporesi, E. (2015) taxonomic and phylogenetic contributions to fungal species. Fungal Diversity 72 (1): 1–197. https://doi.org/10.1007/s13225-015-0324-yMontoya, L., Bandala, V. M. & Garay, E. (2014) Two new species of Lactarius associated with Alnus acuminata subsp. arguta in Mexico. Mycologia 106 (5): 949–962. https://doi.org/10.3852/14-006Montoya, L., Bandala, V.M., Haug, I. & Stubbe, D. (2012) A new species of Lactarius (subgenus Gerardii) from two relict Fagus grandifolia var. mexicana populations in Mexican montane cloud forests. Mycologia 104 (1): 175–181. https://doi.org/10.3852/11-051Nuytinck, J. & Ammirati, J.F. (2014) A new species of Lactarius sect. Deliciosi (Russulales, Basidiomycota) from western North America. Botany 92 (10): 767–774. https://doi.org/10.1139/cjb-2014-0102Sang, X., Li, X., Wang, Y. & Fan, L. (2016) Four new sequestrate species of Russulaceae found in China. Phytotaxa 289 (2): 101–117. https://doi.org/10.11646/phytotaxa.289.2.1Sharma, S., Kaur, M. & Atri, N.S. (2013) Lactarius annulocystidiatus sp. nov. from India. Mycotaxon 121 (1): 63–67. https://doi.org/10.5248/121.63Shi, S.F., Wang, X.H. & Bau, T. (2018) Three new species of Lactarius (Russulaceae, Russulales) from Northeast China. Mycoscience 59 (3): 206–217. https://doi.org/10.1016/j.myc.2017.11.001Song, Y., Li, J.W., Buyck, B., Zheng, J.F. & Qiu, L.H. (2018) Russula verrucospora sp. nov. and R. xanthovirens sp. nov., two novel species of Russula (Russulaceae) from southern China. Cryptogamie Mycologie 39 (1): 129–142. https://doi.org/10.7872/crym/v39.iss1.2018.129Song, Y., Zhang, J.B., Li, J.W. & Qiu, L.H. (2018) Lactifluus sinensis sp. nov. and L. sinensis var. reticulatus var. nov. (Russulaceae) from southern China. Nova Hedwigia 107 (1–2): 91–103. https://doi.org/10.1127/nova_hedwigia/2017/0451Song, Y., Zhang, J.B., Li, J.W., Xia, S.Y. & Qiu, L.H. (2017) Phylogenetic and morphological evidence for Lactifluus robustus sp. nov. (Russulaceae) from southern China. Nova Hedwigia 105 (3–4): 519–528. https://doi.org/10.1127/nova_hedwigia/2017/0431Uniyal, P., Das, K. & Bhatt, R.P. (2018) Two novel species of Lactarius subgenus Plinthogalus from Uttarakhand Himalaya. Phytotaxa 338 (3): 255–264. https://doi.org/10.11646/phytotaxa.338.3.3Verbeken, A. & Nuytinck, J. (2013) Not every milkcap is a Lactarius. Scripta Bot Belgic 51: 162–168.Verbeken, A., Bougher, N.L. & Halling, R. (2002) Lactarius (Basidiomycota, Russulaceae) in Papua New Guinea. 3. Two new Lactarius species in subgenus Plinthogali. Australian Systematic Botany 15 (6): 765–771. https://doi.org/10.1071/SB01036Verbeken, A., Hampe, F., Wissitrassameewong, K., Hyde, K., Eberhardt, U. & Nuytinck, J. (2014) A new angiocarpous Lactarius species from Thailand. Phytotaxa 181 (3): 163–170. https://doi.org/10.11646/phytotaxa.181.3.4Verbeken, A., Stubbe, D., Van de Putte, K., Eberhardt, U. & Nuytinck, J. (2014) Tales of the unexpected: angiocarpous representatives of the Russulaceae in tropical South East Asia. Persoonia: Molecular Phylogeny and Evolution of Fungi 32: 13. https://doi.org/10.3767/003158514X679119Wang, X.H. (2017a) Seven new species of Lactarius subg. Lactarius (Russulaceae) from southwestern China. Mycosystema 36 (11): 1463–1482.Wang, X.H. (2017b) Two New Species of Lactarius subg. Russularia from Subalpine Regions of Southwestern China. Journal of Fungal Research 15 (4): 222–228.Wang, X.H. (2016) Three New Species of Lactarius Sect. Deliciosi from Subalpine-Alpine Regions of Central and Southwestern China. Cryptogamie, Mycologie.Wang, X.H., Nuytinck, J. & Verbeken, A. (2015) Lactarius vividus sp. nov. (Russulaceae, Russulales), a widely distributed edible mushroom in central and southern China. Phytotaxa 231 (1): 63–72. https://doi.org/10.11646/phytotaxa.231.1.6Wisitrassameewong, K., Nuytinck, J., D Hyde, K.E.V.I.N. & Verbeken, A. (2014) Lactarius subgenus Russularia (Russulaceae) in Southeast Asia: 1. Species with very distant gills. Phytotaxa 158 (1): 23–42. https://doi.org/10.11646/phytotaxa.158.1.2Wisitrassameewong, K., Nuytinck, J., Hampe, F., Hyde, K. & Verbeken, A. (2014) Lactarius subgenus Russularia (Russulaceae) in South-East Asia: 2. species with remarkably small basidiocarps. Phytotaxa 188 (4): 181–197. https://doi.org/10.11646/phytotaxa.188.4.1Zhang, J.B., Huang, H. & Qiu, L.H. (2016) Lactifluus dinghuensis sp. nov. from southern China. Nova Hedwigia 102 (1–2): 233–240. https://doi.org/10.1127/nova_hedwigia/2015/0305Zhang, J.B., Li, J.W., Li, F. & Qiu, L.H. (2017) Russula dinghuensis sp. nov. and R. subpallidirosea sp. nov., Two New Species from Southern China Supported by Morphological and Molecular Evidence. Cryptogamie Mycologie 38 (2): 1–13. https://doi.org/10.7872/crym/v38.iss2.2017.191Zheng, G.Y., Lon, T.C., Li, C. & Li, T.H. (1985) Some new species and varieties of Agaricales from Dinghu Mountain. Microbiological Journal (1): 24–30.