Abstract
Suillus marginielevatus sp. nov. and S. triacicularis are reported from Himalayan moist temperate forests of Pakistan in association with conifers. Morphologically S. marginielevatus is close to S. sibiricus, S. granulatus and S. intermedius but it can be distinguished by uplifted pileus margin and curved stipe with no ring at all stages. Phylogenetic analysis of internal transcribed spacer (ITS) region showed that this species form separate clade from other closely related Suillus species. Suillus triacicularis represents a new record for Pakistani mycobiota. Field photographs of fresh basidocarps and line drawings of microcharacters are provided along with phylogenetic tree.