Abstract
Several specimens of Volvopluteus were recently collected from Northwest China during a mushroom survey. Through morphological characterization and phylogenetic analysis based on the nuclear ribosomal DNA internal transcribed spacer region (rDNA ITS), two novel species were identified and described as Volvopluteus longipes and Volvopluteus platensis. Volvopluteus longipes, discovered in the weeds of the Loess Plateau in Gansu Province, is characterized by big basidiomata, long stipes, and large basidiospores; V. platensis was found in the plateau grasslands of Qinghai Province at altitudes exceeding 3000 m, exhibiting smaller basidiomata but relatively large basidiospores. Detailed morphological descriptions, illustrations, phylogenetic analysis results, and a key to the genus are provided.
References
- Barrie, F.R., Buck, W.R., Demoulin, V., Greuter, W.E., Hawksworth, D.L., Herendeen, P.S., Knapp, S., Marhold, K., Prado, J. & Turland, N.J. (2012) International Code of Nomenclature for algae, fungi and plants (Melbourne Code). Koeltz Scientific Books, Germany, 154 pp., pl. 240.
- Boekhout, T. (1990) Volvariella. Flora Agaricina Neerlandica 2: 56–64.
- Bougher, N.L. & Barrett, M.D. (2023) Volvopluteus earlei and Volvariella taylorii: new fungi for Western Australia (Basidiomycota: Agaricales: Pluteaceae). Nuytsia 34: 187–201. https://doi.org/10.58828/nuy01054
- Contu, M. (2007) Volvariella earlei (Basidiomycota, Pluteaceae), nuova per l’Europa, e note sulla tassonomia di Volvariella media sensu J. Lange. Micologia e Vegetazione Mediterranea 21 (29): 101–106.
- Dai, Y.C., Yang, Z.L., Cui, B.K., Wu, G., Yuan, H.S., Zhou, L.W., He, S.H., Ge, Z.W., Wu, F., Wei, Y.L., Yuan, Y. & Si, J. (2021) Diversity and systematics of the important macrofungi in Chinese forests. Mycosystema 40 (10): 770–805. https://doi.org/10.13346/j.mycosystema.210036
- Darriba, D., Taboada, G.L., Doallo, R. & Posada, D. (2012) jModelTest 2: more models, new heuristics and high-performance computing. Nature Methods 9 (8): 772. https://doi.org/10.1038/nmeth.2109
- Edgar, R.C. (2004) MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research 32: 1792–1797. https://doi.org/10.1093/nar/gkh340
- Giannoni, F., Pera, U. & Della Maggiora, M. (2018) Volvopluteus earlei, an uncommon species throughout Europe, new for Tuscany. Micologia Toscani 0 (rev. 2020): 11–25.
- He, M.Q., Zhao, R.L., Hyde, K.D., Begerow, D., Kemler, M., Yurkov, A., McKenzie, E.H.C., Raspé, O., Kakishima, M., Sánchez-Ramírez, S., Vellinga, E.C., Halling, R., Papp, V., Zmitrovich, I.V., Buyck, B., Ertz, D., Wijayawardene, N.N., Cui, B.K., Schoutteten, N., Liu, X.Z., Li, T.H., Yao, Y.J., Zhu, X.Y., Liu, A.Q., Li, G.J., Zhang, M.Z., Ling, Z.L., Cao, B., Antonín, V., Boekhout, T., da Silva, B.D.B., De Crop, E., Decock, C., Dima, B., Dutta, A.K., Fell, J.W., Geml, J., Ghobad-Nejhad, M., Giachini, A.J., Gibertoni, T.B., Gorjón, S.P., Haelewaters, D., He, S.H., Hodkinson, B.P., Horak, E., Hoshino, T., Justo, A., Lim, Y.W., Menolli, N., Mešić, A., Moncalvo, J.M., Mueller, G.M., Nagy, L.G., Nilsson, R.H., Noordeloos, M., Nuytinck, J., Orihara, T., Ratchadawan, C., Rajchenberg, M., Silva-Filho, A.G.S., Sulzbacher, M.A., Tkalčec, Z., Valenzuela, R., Verbeken, A., Vizzini, A., Wartchow, F., Wei, T.Z., Weiß, M., Zhao, C.L. & Kirk, P.M. (2019) Notes, outline and divergence times of Basidiomycota. Fungal diversity 99: 105–367. https://doi.org/10.1007/s13225-019-00435-4
- Hu, Y., Karunarathna, S.C., Li, H., Galappaththi, M.C., Zhao, C.L., Kakumyan, P. & Mortimer, P.E. (2022) The impact of drying temperature on basidiospore size. Diversity 14 (4): 239. https://doi.org/10.3390/d14040239
- Justo, A. & Castro, M.L. (2010) The genus Volvariella in Spain: V. dunensis comb. & stat. nov. and observations on V. earlei. Mycotaxon 112 (1): 261–270.
- Justo, A., Vizzini, A., Minnis, A.M., Menolli, Jr.N., Capelari, M., Rodríguez, O., Malysheva, E., Contu, M., Ghignone, S. & Hibbett, D.S. (2011a) Phylogeny of the Pluteaceae (Agaricales, Basidiomycota): taxonomy and character evolution. Fungal Biology 115 (1): 1–20. https://doi.org/10.1016/j.funbio.2010.09.012
- Justo, A., Minnis, A.M., Ghignone, S., Menolli, N., Pileuselari, M., Rodríguez, O., Della Maggiora, M. & Vizzini, A. (2011b) Species recognition in Pluteus and Volvopluteus (Pluteaceae, Agaricales): morphology, geography and phylogeny. Mycological Progress 10: 453–479. https://doi.org/10.1007/s11557-010-0716-z
- Kaur, M. & Singh, Y. (2014) Family Pluteaceae in north west India. In Proceedings of 8th international conference on mushroom biology and mushroom products 1: 55–70.
- Le, H.T. & Chu, N.T. (2018) New records of genus Volvariella (Pluteaceae) from Cuc Phuong National Park. Vietnam Journal of Science, Technology and Engineering 60 (3): 81–88. https://doi.org/10.31276/VJSTE.60(3).81
- LI, C.H., ZHANG, M., LI, J.P. & LI, T.H. (2025) Volvopluteus deliciosus sp. nov. (Pluteaceae, Agaricales), a new edible species from Xinjiang, China. Phytotaxa 683 (3): 253–262. https://doi.org/10.11646/phytotaxa.683.3.6
- Montoya, L., Bandala, V.M. & Esqueda, M. (2021) Volvopluteus canalipes comb. nov. (Pluteaceae) from the Sonoran Desert of Mexico. Phytotaxa 505: 275–285. https://doi.org/10.11646/phytotaxa.505.3.3
- Rathnayaka, A.R., Tennakoon, D.S., Jones, G.E., Wanasinghe, D.N., Bhat, D.J., Priyashantha, A.H., Stephenson, S.L., Tibpromma, S. & Karunarathna, S.C. (2025) Significance of precise documentation of hosts and geospatial data of fungal collections, with an emphasis on plant-associated fungi. New Zealand Journal of Botany 63 (2–3): 462–489. https://doi.org/10.1080/0028825X.2024.2381734
- Rao, G., Dai, D., Zhang, B. & Li, Y. (2021) A new species of Volvopluteus from China: A new record for the genus. Microbiology China 48 (10): 3791–3798.
- Ronquist, F. & Huelsenbeck, J.P. (2003) MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19 (12): 1572–1574. https://doi.org/10.1093/bioinformatics/btg180
- Senthilarasu, G., Sharma, R. & Singh, S.K. (2012) A new species of Volvariella from India. Mycotaxon 119 (1): 467–476. https://doi.org/10.5248/119.467
- Shaffer, R.L. (1957) Volvariella in North America. Mycologia 49 (4): 545–579. https://doi.org/10.1080/00275514.1957.12024669
- Singer, R. (1986) The Agaricales in Modern Taxonomy, 4th Edition. Koeltz Scientific Books, Königstein, Germany.
- Stamatakis, A. (2014) RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 30 (9): 1312–1313. https://doi.org/10.1093/bioinformatics/btu033
- Takehashi, S., Hoshina, T. & Kasuya, T. (2010) Taxonomic Studies on Agaricales of Hokkaido. In: Northern Japan, with special references to Melanoleuca, Oudemansiella, Xerula, Volvariella, and Pluteus. NPO The Forum of Fungi in Northern Japan, Sapporo.
- Turland, N.J., Wiersema, J.H., Barrie, F.R., Greuter, W., Hawksworth, D.L., Herendeen, P.S., Knapp, S., Kusber, W.H., Li, D.Z., Marhold, K., May, T.W., McNeill, J., Monro, A.M., Prado, J., Price, M.J. & Smith, G.F. (eds.) (2018) International Code of Nomenclature for algae, fungi, and plants (Shenzhen Code) adopted by the Nineteenth International Botanical Congress, Shenzhen, China, July 2017. Regnum Vegetabile 159. Koeltz Botanical Books, Glashütten. https://doi.org/10.12705/Code.2018
- White, T.J., Bruns, T., Lee, S.S. & Taylor, J. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis, M.A., Gelfand, D.H., Sninsky, J.J. & White, T.J. (Eds.) PCR protocols: a guide to methods and applications. Academic Press, San Diego, pp. 315–322. https://doi.org/10.1016/B978-0-12-372180-8.50042-1
- Xu, J., Li, T.H., Shen, Y.H. & Zhang, M. (2015) Volvariella rava sp. nov. from southern China. Mycotaxon 130: 857–865. https://doi.org/10.5248/130.857
- Zheng, D.G., Lu, W.H., Han, M.Y., Dai, D.Q., Zhang, L.J., Elgorban, A.M., Kumla, J., Suwannarach, N., Tibpromma, S., Chukeatirote, E. & Karunarathna, S.C. (2025) Volvopluteus yunnanensis (Pluteaceae, Agaricales), a novel species from Yunnan Province, China. Phytotaxa 706 (3): 269–280. https://doi.org/10.11646/phytotaxa.706.3.5
- Zhu, X.T., Zhang, J.M., Zhang, G.Q., Fan, J.X. & Dang, Y.X. (2024) Taxonomic description of a new species of Leucoagaricus and utilization of the Pantone Connect app in classification research. Phytotaxa 642 (1): 084–094. https://doi.org/10.11646/phytotaxa.642.1.7
