Skip to main content Skip to main navigation menu Skip to site footer
Responsive image
Issue: Vol. 666 No. 1: 26 Sept. 2024
Type: Article
Published: 2024-09-26
DOI: 10.11646/phytotaxa.666.1.1
Page range: 1-16
Abstract views: 187
PDF downloaded: 10

Two new species and a new record of Entolomatoid fungi (Entolomataceae, Agaricales) from Pakistan

Fungal Biology and Systematics Research Laboratory, Institute of Botany, University of the Punjab, Quaid-e-Azam Campus-54590, Lahore, Pakistan
Fungal Biology and Systematics Research Laboratory, Institute of Botany, University of the Punjab, Quaid-e-Azam Campus-54590, Lahore, Pakistan
Fungal Biology and Systematics Research Laboratory, Institute of Botany, University of the Punjab, Quaid-e-Azam Campus-54590, Lahore, Pakistan
Fungal Biology and Systematics Research Laboratory, Institute of Botany, University of the Punjab, Quaid-e-Azam Campus-54590, Lahore, Pakistan
Fungal Biology and Systematics Research Laboratory, Institute of Botany, University of the Punjab, Quaid-e-Azam Campus-54590, Lahore, Pakistan
Claudopus pink-spored phylogenetic affinities taxonomy Fungi

Abstract

Taxonomic investigations on the family Entolomataceae were conducted in Pakistan from 2018 to 2022. Morphological characteristics and molecular phylogenetic analyses using ITS and LSU sequences revealed two new species, Entoloma pakistanicum and Rhodophana margallensis, and a new record, E. albotomentosum. Rhodophana margallensis is the first report of the genus Rhodophana from Pakistan. We present illustrated descriptions of the three species and comparison with morphologically similar or related species. Furthermore, we provide a key to the known species of Entoloma from Pakistan.

References

  1. Acharya, K., Paloi, S., Sikder, R., Dutta, A.K. & Roy, A. (2017) Entoloma albotomentosum (Entolomataceae): First report from India based on morphological and molecular (ITS sequence) data. Current Research in Environmental & Applied Mycology 7 (1): 40‒47. https://doi.org/10.5943/cream/7/1/6
  2. Ahmad, S., Iqbal, S.H. & Khalid, A.N. (1997) Fungi of Pakistan. Sultan Ahmad Mycological Society of Pakistan, Lahore, pp. 1–248.
  3. Arnolds, E. & Veerkamp, M. (2008) Basisrapport Rode Lijst Paddenstoelen. Nederlandse Mycologische Vereniging, Utrecht, pp. 295.
  4. Asif, M., Saba, M., Akram, W., Maula, F. & Raza, M. (2024) Integrated morphological and molecular approaches unveil a new species and a new record of the genus Entoloma (Entolomataceae) from southern Punjab, Pakistan. Phytotaxa 653 (3): 219–240. https://doi.org/10.11646/phytotaxa.653.3.3
  5. Badshah, L., Hussain, F. & Sher, Z. (2016) Floristic inventory, ecological characteristics and biological spectrum of plants of Parachinar, Kurram agency, Pakistan. Pakistan Journal of Botany 48 (4): 1547–1558.
  6. Baroni, T.J. & Matheny, P.B. (2011) A re-evaluation of gasteroid and cyphelloid species of Entolomataceae from Eastern North America. Harvard Papers in Botany 16: 293–310. https://doi.org/10.3100/0.25.016.0205
  7. Baroni, T.J., Hofstetter, V., Largent, D.L. & Vilgalys, R. (2011) Entocybe is proposed as a new genus in the Entolomataceae (Agaricales, Basidiomycota) based on morphological and molecular evidence. North American Fungi 6: 1–19. https://doi.org/10.2509/naf2011.006.012
  8. Brandrud, T.E., Bendiksen, E., Jordal, J.B., Weholt, Ø., Eidissen, S.E., Lorås, J.A., Dima, B. & Noordeloos, M.E. (2018) Entoloma species of the rhodopolioid clade (subgenus Entoloma; Tricholomatinae, Basidiomycota) in Norway. Agarica 38: 21–46. https://doi.org/10.5617/agarica.11643
  9. Bruns, T.D. (1995) Thoughts on the processes that maintain local species diversity of ectomycorrhizal fungi. The significance and regulation of soil biodiversity, Springer Netherlands, pp. 63–73. https://doi.org/10.1007/978-94-011-0479-1_5
  10. Buyck, B., Eyssartier, G., Dima, B., Consiglio, G., Noordeloos, M.E., Papp, V., Bera, I., Ghosh, A., Rossi, W., Leonardi, M. & Das, K. (2021) Fungal Biodiversity Profiles 101-110. Cryptogamie, Mycologie 42 (5): 63–89. https://doi.org/10.5252/cryptogamie-mycologie2021v42a5
  11. Castellano, M.A., Trappe, J.M. & Vernes, K. (2011) Australian species of Elaphomyces (Elaphomycetaceae, Eurotiales, Ascomycota). Australian Systematic Botany 24: 32–57. https://doi.org/10.1071/SB10012
  12. Co-David, D., Langeveld, D. & Noordeloos, M.E. (2009) Molecular phylogeny and spore evolution of Entolomataceae. Persoonia-Molecular Phylogeny and Evolution of Fungi, 23 (1): 147–176. https://doi.org/10.3767/003158509X480944
  13. Dima, B., Brandrud, T.E., Corriol, G., Jansen, G.M., Jordal, J.B., Khalid, A.N., Larsson, E., Lorås, J.A., Morozova, O.V., Naseer, A. & Noordeloos, M.E. (2021) Fungal systematics and evolution: FUSE 7. Sydowia 73: 271–340. https://doi.org/10.12905/0380.sydowia73-2021-0271
  14. Edgar, R.C. (2004) MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research 32: 1792– 1797. https://doi.org/10.1093/nar/gkh340
  15. Elliott, T.F., Nelsen, D.J., Karunarathna, S.C. & Stephenson, S.L. (2020) Entoloma sequestratum, a new species from northern Thailand, and a worldwide key to sequestrate taxa of Entoloma (Entolomataceae). Fungal Systematics and Evolution 6: 253–263. https://doi.org/10.3114/fuse.2020.06.12
  16. Fatima, N., Usman, M. & Khalid, A.N. (2023) Entoloma punjabensis sp. nov. of Rusticoides group from Chichawatni Forest, Pakistan. Phytotaxa 612: 217–228. https://doi.org/10.11646/phytotaxa.612.2.6
  17. Gates, G.M. & Noordeloos, M.E. (2007) Preliminary studies in the genus Entoloma in Tasmania – I. Persoonia 19: 157–226.
  18. Geml, J., Timling, I., Robinson, C.H., Lennon, N., Nusbaum, H.C., Brochmann, C., Noordeloos, M.E. & Taylor, D.L. (2012) An arctic community of symbiotic fungi assembled by long‐distance dispersers: phylogenetic diversity of ectomycorrhizal basidiomycetes in Svalbard based on soil and sporocarp DNA. Journal of Biogeography 39 (1): 74–88.
  19. Haelewaters, D., Dima, B., Abdel-Hafiz, A.I., Abdel-Wahab, M.A., Abul-Ezz, S.R., Acar, I., Aguirre-Acosta, E., Aime, M.C., Aldemir, S., Ali, M. & Ayala-Vasquez, O. (2020) Fungal systematics and evolution: FUSE 6. Sydowia 72: 231–356.
  20. Halama, M. (2011) Entoloma albotomentosum (Agaricales, Basidiomycota), a species new to Poland. Acta Mycologica 46 (2): 129–136. https://doi.org/10.5586/am.2011.009
  21. Hall, T.A. (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 41: 95–98. https://doi.org/10.1073/pnas.1117018109
  22. Hartley, A.J., de Mattos-Shipley, K., Collins, C.M., Kilaru, S., Foster, G.D. & Bailey, A.M. (2009) Investigating pleuromutilin-producing Clitopilus species and related basidiomycetes. FEMS Microbiology Letters 297 (1): 24–30. https://doi.org/10.1111/j.1574-6968.2009.01656.x
  23. He, X.L., Peng, W.H. & Gan, B.C. (2014) Morphological and molecular evidence for a new species in Entoloma subgenus Claudopus from Sichuan Province, southwest China. Mycoscience 56 (3): 326–331. https://doi.org/10.3897/mycokeys.61.46446
  24. Hofstetter, V., Clémençon, H., Vilgalys, R. & Moncalvo, J.M. (2002) Phylogenetic analyses of the Lyophylleae (Agaricales, Basidiomycota) based on nuclear and mitochondrial rDNA sequences. Mycological Research 106 (9): 1043–1059. https://doi.org/10.1017/S095375620200641X
  25. Iqbal, M., Khan, S.M., Ahmad, Z., Hussain, M., Shah, S.N., Kamran, S., Manan, F., Haq, Z.U. & Ullah, S. (2021) Vegetation classification of the Margalla Foothills, Islamabad under the influence of edaphic factors and anthropogenic activities using modern ecological tools. Pakistan Journal of Botany 53 (5): 1831–1843. https://doi.org/10.30848/PJB2021-5(22)
  26. Izhar, A., Qasim, T., Asif, M., Niazi, A.R. & Khalid, A.N. (2023) Entoloma griseofibrillosus (Entolomataceae, Basidiomycota), a new species from Punjab, Pakistan. Phytotaxa 589 (1): 1–14. https://doi.org/10.11646/phytotaxa.589.1.1
  27. Izhar, A., Usman, M., Kiran, M. & Khalid, A.N. (2022) Two new species of Entoloma (Entolomataceae) from Khyber Pakhtunkhwa, Pakistan. Phytotaxa 574 (3): 226–240. https://doi.org/10.11646/phytotaxa.574.3.2
  28. Jabeen, A., Khan, M.A., Ahmad, M., Zafar, M. & Ahmad, F. (2009) Indigenous uses of economically important flora of Margallah Hills National Park, Islamabad, Pakistan. African Journal of Biotechnology 8 (5): 763–784.
  29. Jančovičová, S. & Adamčík, S. (2014) Exploring the limits of morphological variability and ecological preferences of Entoloma albotomentosum. Czech Mycology 66 (2): 121–134. https://doi.org/10.33585/cmy.66202
  30. Karstedt, F. & Capelari, M. (2013) Inocephalus (Entolomataceae, Agaricales) from São Paulo State, Brazil. Nova Hedwigia 96: 279–308. https://doi.org/10.1127/0029-5035/2012/0055
  31. Khalid, A.N. (2022) A Checklist of Macrofungi of Pakistan published from 1998–2020. Pakistan Journal of Botany 54: 1947–1962. https://doi.org/10.30848/PJB2022-5(23)
  32. Kirk, P. (2019) Species Fungorum. In: Roskov, Y., Ower, G., Orrell, T., Nicolson, D., Bailly, N., Kirk, P.M., Bourgoin, T., DeWalt, R.E., Decock, W., Nieukerken, E., Zarucchi, J. & Penev, L. (Eds.) Species 2000 & ITIS Catalogue of Life, 2019 Annual Checklist. Available from: www.catalogueoflife.org/annual-checklist/2019 (accessed 25 September 2024)
  33. Kokkonen, K. (2015) A survey of boreal Entoloma with emphasis on the subgenus Rhodopolia. Mycological Progress 14: 1–116. https://doi.org/10.1007/s11557-015-1135-y
  34. Kluting, K.L., Baroni, T.J. & Bergemann, S.E. (2014) Toward a stable classification of genera within the Entolomataceae: a phylogenetic re-evaluation of the Rhodocybe-Clitopilus clade. Mycologia 106: 1127–1142. https://doi.org/10.3852/13-270
  35. Kondo, K., Nakamura, K., Ishigaki, T., Sakata, K., Obitsu, S., Noguchi, A., Fukuda, N., Nagasawa, E., Teshima, R. and Nishimaki-Mogami, T. (2017) Molecular phylogenetic analysis of new Entoloma rhodopolium-related species in Japan and its identification method using PCR-RFLP. Scientific Reports 7 (1): 14942. https://doi.org/10.1038/s41598-017-14466-x
  36. Krieglsteiner, G.J. (2003) Entolomataceae. In: Krieglsteiner, G.J. (Ed.) Die Großpilze Baden-Württembergs. 4. Ständerpilze: Blätterpilze. II. Verlag Eugen Ulmer GmbH & Co., Stuttgart, pp. 138–243.
  37. Kühner, R. (1947) Agaricus (Clitocybe) hirneolus Fries, champignon souvent me'connu en France aujourd'hui et la tribu nouvelles des Orcelle's. Bulletin trimestriel de la Société mycologique de France 62: 183–193.
  38. Kühner, R. & Lamoure, D. (1971) Agaricales de la zone alpine. Genre Rhodocybe R. maire. Bulletin trimestriel de la Société mycologique de France 87: 15–23.
  39. Largent, D.L. (1994) Entolomatoid fungi of the western United States and Alaska. Mad River Press, Eureka, California.
  40. Legon, N.W., Henrici, A., Roberts, P., Spooner, B.M. & Watling, R. (2005) Checklist of the British and Irish Basidiomycota. Royal Botanic Gardens, Kew, pp. 536.
  41. Miller, M.A., Pfeiffer, W. & Schwartz, T. (2010) Creating the CIPRES Science Gateway for inference of large phylogenetic trees. In: Proceedings of the Gateway computing environments workshop (GCE). New Orleans, Louisiana, pp. 1–8. https://doi.org/10.1109/GCE.2010.5676129.
  42. Morgado, L.N., Noordeloos, M.E. & Hausknecht, A. (2016) Clitopilus reticulosporus, a new species with unique spore ornamentation, its phylogenetic affinities and implications on the spore evolution theory. Mycological Progress 15: 1–8. https://doi.org/10.1007/s11557-016-1165-0
  43. Munsell (1975) Munsell soil color charts. Macbeth Division of Kollmorgen Corporation. Baltimore, Maryland.
  44. Musumeci, E. (2020) Una nueva especie de Rhodophana (antes Rhodocybe) para el territorio Alsaciano. Errotari, pp. 224–236.
  45. Noordeloos, M.E. (2008) Entoloma (Fr.) P. Kumm. In: Knudsen, H. & Vesterholt, J. (Eds.) Funga nordica. Agaricoid, boletoid and cyphelloid genera. Nordsvamp, Copenhagen: pp. 433–491.
  46. Noordeloos, M.E. & Hausknecht, A. (1989) Über einige neue und interessante Rötlinge aus Österreich. Zeitschrift für. Mykologie 55 (1): 31–42.
  47. Noordeloos, M.E. (1988) Entolomataceae Kotl. & P. Flora Agaricina Neerlandica 1: 77–177.
  48. Noordeloos, M.E. & Hausknecht, A. (2007) The genus Entoloma (Basidiomycetes, Agaricales) of the Mascarenes and Seychelles. Fungal Diversity 27 (1): 111–144.
  49. Noordeloos, M.E. & Morozova, O.V. (2010) New and noteworthy Entoloma species from the Prismorsky Territory, Russian Far East. Mycotaxon 112: 231–255.
  50. Noordeloos ME (2004) Entoloma s.l. Fungi Europaei 5a: 761–1378.
  51. Noordeloos, M.E. & Gates, G.M. (2012) The Entolomataceae of Tasmania. Fungal diversity research series, vol. 22. Springer, London. pp. 399. https://doi.org/10.1007/978-94-007-4679-4
  52. Noordeloos, M.E., Weholt, Ø., Bendiksen, E., Brandrud, T.E., Eidissen, S.E., Lorås, J., Morozova, O. & Dima, B. (2018) Entoloma aurorae-borealis sp. nov. and three rare Entoloma species in the Sinuatum clade (subg. Entoloma) from northern Europe. Sydowia 70: 199–210.
  53. Noordeloos, M.E., Lorås, J.A., Eidissen, S.E., Brandrud, T.E., Bendiksen, E., Morozova, O., Jordal, J.B., Weholt, Ø., Jansen, G.M., Larsson, E. & Bálint, D. (2021) Three new Entoloma species of the Cyanula clade from (sub)alpine habitats in Northern Norway and Sweden. Sydowia 73: 185–196. https://doi.org/10.12905/0380.sydowia73-2020-0185
  54. Pegler, D.N. (1977) Entolomataceae (Agaricales) from India & Sri Lanka. Kew Bulletin 32: 189–220.
  55. Raj, K.A., Latha, K.P.D., Iyyappan, R. & Manimohan, P. (2015) Rhodophana squamulosa—a new species of Entolomataceae from India. Mycoscience 57 (2): 90–95. https://doi.org/10.1016/j.myc.2015.10.001
  56. Rambaut, A. (2014) FigTree 1.4.2 software. Institute of Evolutionary Biology, University of Edinburgh.
  57. Rambaut, A., Suchard, M.A. & Xie, D. (2022) Tracer v. 1.6. Available from: http://beast.bio.ed.ac.uk/Tracer (accessed 25 April 2022)
  58. Reschke, K., Noordeloos, M.E., Manz, C., Hofmann, T.A., Rodríguez-Cedeño, J., Dima, B. & Piepenbring, M. (2022) Fungal diversity in the tropics: Entoloma spp. in Panama. Mycological Progress 21 (1): 93–145. https://doi.org/10.1007/s11557-021-01752-2
  59. Smith, M.E., Amses, K.R., Elliott, T.F., Obase, K, Aime, M.C. & Henkel, T.W. (2015) New sequestrate fungi from Guyana: Jimtrappea guyanensis gen. sp. nov., Castellanea pakaraimophila gen. sp. nov., and Costatisporus cyanescens gen. sp. nov. (Boletaceae, Boletales). IMA Fungus 6: 297–317. https://doi.org/10.5598/imafungus.2015.06.02.03
  60. Shirazi, S.A. (2012) Temporal Analysis of Land Use and Land Cover Changes in Lahore-Pakistan. Pakistan Vision 13 (1): 188–206.
  61. Stamatakis, A. (2014) RAxML Version 8: A tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 30 (9): 1312–1313. https://doi.org/10.1093/bioinformatics/btu033
  62. Sultana, K., Rauf, C.A., Riaz, A., Naz, F., Irshad, G. & Haque, M.I. (2011) Checklist of Agarics of Kaghan Valley-1. Pakistan Journal of Botany 43: 1777–1787.
  63. Sulzbacher, M.A., Orihara, T., Grebenc, T., Wartchow, F., Smith, M.E., Martín, M.P., Giachini, A.J. & Baseia, I.G. (2020) Longistriata flava (Boletaceae, Basidiomycota) – a new monotypic sequestrate genus and species from Brazilian Atlantic Forest. MycoKeys 62: 53–73. https://doi.org/10.3897/mycokeys.62.39699
  64. Vilgalys, R. & Hester, M. (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. Journal of Bacteriology 172 (8): 4239–4246. https://doi.org/10.1128/JB.172.8.4238-4246.1990
  65. Vizzini, A., Dähncke, R.M. & Contu, M. (2011a) Clitopilus rubroparvulus (Basidiomycota, Agaricomycetes), a new species from the Canary Islands (Spain). Mycosphere 2: 291–295.
  66. Vizzini, A., Musumeci, E., Ercole, E. & Contu, M. (2011b) Clitopilus chrischonensis sp. nov. (Agaricales, Entolomataceae), a striking new fungal species from Switzerland. Nova Hedwigia 92 (3): 425. https://doi.org/10.1127/0029-5035/2011/0092-0425
  67. Vizzini, A., Dähncke, R.M. & Contu, M. (2012) Clitopilus canariensis (Basidiomycota, Entolomataceae), a new species in the C. nitellinus-complex (Clitopilus subg. rhodophana) from the Canary Islands (Spain). Brittonia 63: 484–488. https://doi.org/10.1007/s12228-011-9187-z
  68. Vizzini, A., Picillo, B., Ercole, E., Vila, J. & Contu, M. (2016) Rhodocybe formosa (Agaricales, Entolomataceae): new collections, molecular data and synonymy, and Rhodocybe griseonigrella comb. nov. Phytotaxa 255 (1): 34–46. https://doi.org/10.11646/phytotaxa.255.1.3
  69. Wang, J.R. & Bau, T. (2013) A new species and a new record of the genus Entoloma from China. Mycotaxon 124 (1): 165–171. https://doi.org/10.5248/124.165
  70. White, T.J., Bruns, T.D. & Taylor, L.J. (1990) Amplification direct sequencing of fungal ribosomal RNA genes for Phylogenetics. In: Innis, M.A., Gelf, D.H., Sninsky, J.J. & White, T.J. (Eds.) PCR protocols: A guide to methods applications. Academic Press, New York, pp. 315–322. https://doi.org/10.1016/b978-0-12-372180-8.50042-1
  71. Xu, J., Jiang, Y., Wang, T., Zhang, D., Li, X. & Hosen, M.I. (2023) Morphological characteristics and phylogenetic analyses revealed four new species of Agaricales from China. Frontiers in Microbiology 14: 1118525. https://doi.org/10.3389/fmicb.2023.1118525
  72. Yang, C. & Fan, L. (2020) Rhodophana guandishanense (Entolomataceae, Agaricales), a new species from China. Phytotaxa 455: 173–181. https://doi.org/10.11646/phytotaxa.455.2.9