Chaetomium albiziae, a new endophytic species from Albizia lebbeck in Iran

MEHDI MEHRABI-KOUSHKI; ATENA SAFI

doi:10.11646/phytotaxa.591.2.5

Issue: Vol. 591 No. 2: 4 April 2023

Type: Article

Published: 2023-04-04

DOI: 10.11646/phytotaxa.591.2.5

Page range: 137-146

Abstract views: 232

PDF downloaded: 4

Chaetomium albiziae, a new endophytic species from Albizia lebbeck in Iran

MEHDI MEHRABI-KOUSHKI⁺⁻
ATENA SAFI⁺⁻

Department of Plant Protection, Faculty of Agriculture, Shahid Chamran University of Ahvaz, Ahvaz, Khuzestan Province, Iran. Biotechnology and Bioscience Research Center, Shahid Chamran University of Ahvaz, Ahvaz, Iran.

Department of Plant Protection, Faculty of Agriculture, Shahid Chamran University of Ahvaz, Ahvaz, Khuzestan Province, Iran

Chaetomicaceae endophyte molecular phylogeny morphology silk tree Fungi

Requires Subscription or Fee PDF (2MB)

Abstract

The silk tree (Albizia lebbeck (L.) Benth) is one of the ornamental plants in Ahvaz (southwest of Iran). Two endophytic fungal strains, isolated from the leaves of this plant, were characterized. Molecular phylogenetic analyses based on a combined matrix of the internal transcribed spacer regions 1 and 2 including the intervening 5.8S nuclear ribosomal DNA (ITS), and partial regions of the β-tubulin (tub2) and RNA polymerase II second largest subunit (rpb2) indicated that both strains represent a new Chaetomium species, C. albiziae sp. nov. in the family Chaetomiaceae. Chaetomium albiziae formed a sister lineage with sterile species C. cucumericola. This new species can be characterized by ostiolate perithecia with septate lateral and terminal setae, clavate asci and aseptate ellipsoidal to ovoid ascospores with an apical germ pore. In addition, this is the first report of the endophytic association of the genus Chaetomium with Albizia lebbeck worldwide.

References

Aghyl, H., Mehrabi-Koushki, M. & Esfandiari, M. (2020) Chaetomium iranicum and Collariella capillicompacta spp. nov. and notes to new hosts of Amesia species in Iran. Sydowia 73: 21–30.
Ahmadpour, S.A., Mehrabi-Koushki, M. & Farokhinejad, R. (2017) Neodidymelliopsis farokhinejadii, a new fungal species from dead branches of trees in Iran. Sydowia 69: 171–182.
Ahmed, S.A., Khan, Z., Wang, X., Moussa, T.A.A, Al-Zahrani, H.S., Almaghrabi, O.A., Sutton, D.A., Ahmad, S., Groenewald, J.Z., Alastruey-Izquierdo, A., van Diepeningen, A., Menken, S.B.J., Najafzadeh, M.J., Crous, P.W., Cornely, O., Hamprecht, A., Vehreschild, M.J.G.T., Kindo, A.J. & de Hoog, G.S. (2016) Chaetomium-like fungi causing opportunistic infections in humans: a possible role for extremotolerance. Fungal Diversity 76 (1): 11–26. https://doi.org/10.1007/s13225-015-0338-5
Ali, B.Z. & Alfayed, A.A. (2017) Endophytic fungi from leaves and twigs of Albizia lebbeck and their antifungal activity. Ibn Al-Haitham Journal for Pure and Applied Sciences 27: 24–33.
Ames, L.M. (1963) A Monograph of the Chaetomiaceae. Research and Development Service of U.S. Army, UAS.
Asgari, B. & Zare, R. (2011) The genus Chaetomium in Iran, a phylogenetic study including six new species. Mycologia 103: 863–882. https://doi.org/10.3852/10-349
Babaahmadi, G., Mehrabi-Koushki, M. & Hayati, J. (2018) Allophoma hayatii sp. nov., an undescribed pathogenic fungus causing dieback of Lantana camara in Iran. Mycological Progress 17:365–379. https://doi.org/10.1007/s11557-017-1360-7
Bruns, T.D., White, T.J. & Taylor, J.W. (1991) Fungal molecular systematics. Annual Review of Ecology and Systematics 22: 525–564. https://doi.org/10.1146/annurev.es.22.110191.002521
Darriba, D., Taboada, G.L., Doallo, R. & Posada, D. (2012) jModelTest 2: more models, new heuristics and parallel computing. Nature Methods 9: 772. https://doi.org/10.1038/nmeth.2109
Edler, D., Klein, J., Antonelli, A. & Silvestro, D. (2019) raxmlGUI 2.0 beta: a graphical interface and toolkit for phylogenetic analyses using RAxMl. bioRxiv. https://doi.org/10.1101/800912
Ershad, D. (2009) Fungi of Iran, 3rd edn. Iranian Research Institute of Plant Protection, Tehran. 
Farr, D.F. & Rossman, A.Y. (2021) Fungal Databases: U.S. National Fungus Collections, ARS, USDA. Internet Resource: https://nt.ars-grin.gov/fungaldatabases/ (Retrieved 17 August 2022).
Gioia, L., d’Errico, G., Sinno, M., Ranesi, M., Woo, S.L. & Vinale, F. (2020) A survey of endophytic fungi associated with high-risk plants imported for ornamental purposes. Agriculture 10: 643.
Hall, T.A. (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for windows 95/98/NT. Nucleic Acids Symposium Series 41: 95–98.
Hawksworth, D.L. & Wells, H. (1973) Ornamentation on the terminal hairs in Chaetomium Kunze ex Fr. and some allied genera. Mycological Papers 134: 1–24.
Hess, W.M., Muller, E. & Aue, R. (1967) Germ pores of Chaetomium ascospores. Naturwissenschaften 19: 521–522. https://doi.org/10.1007/BF01129384
Kunze, G. & Schmidt, J.K. (1817) Mykologische Hefte,: 1. Germany, Leipzig Lagace J, Cellier E. A case report of a mixed Chaetomium globosum/ Trichophyton mentagrophytes onychomycosis. Medical Mycology Case Reports 1: 76–78. https://doi.org/10.1016/j.mmcr.2012.09.001
Larki, R., Mehrabi-Koushki, M., Farokhinejad, R. (2019) Identification of Chaetomium and Amesia species associated with different diseases of some herbaceous ornamentals in Ahwaz. Biological Journal of Microorganism 8 (31): 33–50.
Liu, Y.J., Whelen, S. & Hall, B.D. (1999) Phylogenetic relationships among ascomycetes: evidence from an RNA polymerse II subunit. Molecular Biology and Evolution 16: 1799–1808. https://doi.org/10.1093/oxfordjournals.molbev.a026092
Mason-Gamer, R. & Kellogg, E. (1996) Testing for phylogenetic conflict among molecular datasets in the tribe Tiriceae (Graminae). Systematic Biology 45: 524–545. https://doi.org/10.1093/sysbio/45.4.524
Mathur, P., Ghosh, U., Chetani, R., Chaturvedi, P., Sharma, C. & Bhatnagar, P. (2022) Enzymatic bioprospection of endophyticAspergillus niger isolated from Albizia lebbeck (L.) Benth. South African Journal of Botany 148: 580–587. https://doi.org/10.1016/j.sajb.2022.05.033
Millner, P.D., Motta, J.J. & Lentz, PL. (1977) Ascospores, germ pores, ultrastructure, and thermophilism of Chaetomium. Mycologia 69: 720–733. https://doi.org/10.1080/00275514.1977.12020116
O’Donnell, K. & Cigelnik, E. (1997) Two divergent intragenomic rDNA ITS2 types within a monophyletic lineage of the fungus Fusarium are nonorthologous. Molecular Phylogenetics and Evolution 7: 103–116. https://doi.org/10.1006/mpev.1996.0376
Raeder, U. & Broda, P. (1985) Rapid preparation of DNA from filamentous fungi. Letters in Applied Microbiology 1: 17–20. https://doi.org/10.1111/j.1472-765X.1985.tb01479.x
Rashmi, M., Kushveer, J.S. & Sarma, V.V. (2019) A worldwide list of endophytic fungi with notes on ecology and diversity. Mycosphere 10 (1): 798–1079. https://doi.org/10.5943/mycosphere/10/1/19
Ronquist, F., Teslenko, M., Van Der Mark, P., Ayres, D.L., Darling, A., Höhna, S., Larget, B., Liu, L., Suchard, M.A. & Huelsenbeck, J.P. (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology 61: 539–542. https://doi.org/10.1093/sysbio/sys029
Safi, A., Mehrabi-Koushki, M. & Farokhinejad, R. (2020) Amesia khuzestanica and Curvularia iranica spp. nov. from Iran. Mycological Progress 19: 935–945. https://doi.org/10.1007/s11557-020-01612-5
Sharma, N., Kushwaha, M., Arora, D., Jain, S., Singamaneni, V., Sharma, S., Shankar, R., Bhushan, S., Gupta, P.  & Jaglan, S. (2018) New cytochalasin from Rosellinia sanctae-cruciana, an endophytic fungus of Albizia lebbeck. Journal of Applied Microbiology 125 (1): 111–120. https://doi.org/10.1111/jam.13764
Skolko, A.J. & Groves, J.W. (1953) Notes on seed-borne fungi VII. Chaetomium. Canadian Journal of Botany 31: 779–809. https://doi.org/10.1139/b53-060
Sung, G.H., Sung, J.M., Hywel-Jones, N.L. & Spatafora, J.W. (2007) A multi-gene phylogeny of Clavicipitaceae (Ascomycota, Fungi): identification of localized incongruence using a combinational bootstrap approach. Molecular Phylogenetics and Evolution 44: 1204–1223. https://doi.org/10.1016/j.ympev.2007.03.011
Tamura, K., Stecher, G., Peterson, D., Filipski, A. & Kumar, S. (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution 30: 2725–2729. https://doi.org/10.1093/molbev/mst197
von Arx, J.A., Guarro, J. & Figueras, M.J. (1986) The Ascomycete genus Chaetomium. Beihefte Zur nova Hedwigia 84: 1–162.
Wang, X.W., Houbraken, J., Groenewald, J.Z., Meijer, M., Andersen, B., Nielsen, K.F., Crous, P.W. & Samson, R.A. (2016a) Diversity and taxonomy of Chaetomium and chaetomium-like fungi from indoor environments. Studies in Mycology 84:145–224. https://doi.org/10.1016/j.simyco.2016.11.005
Wang, X.W., Lombard, L., Groenewald, J.Z., Li, J., Videira, S.I.R., Samson, R.A., Liu, X.Z. & Crous, P.W. (2016b) Phylogenetic reassessment of the Chaetomium globosum species complex. Persoonia 36: 83–133. https://doi.org/10.3767/003158516X689657
Wang, X.W., Bai, F.Y., Bensch, K., Meijer, M., Sun, B.D., Han, Y.F., Crous, P.W., Samson, R.A., Yang, F.Y. & Houbraken, J. (2019a) Phylogenetic re-evaluation of Thielavia with the introduction of a new family Podosporaceae. Studies in Mycology 93: 155–253. https://doi.org/10.1016/j.simyco.2019.08.002
Wang, X.W., Yang, F.Y., Meijer, M., Kraak, B., Sun, B.D., B.D., Jiang, Y.L., Wu, Y.M., Bai, F.Y., Seifert, K.A., Crous, P.W., Samson, R.A. & Houbraken, J.  (2019b) Redefining Humicola sensu stricto and related genera in the Chaetomiaceae. Studies in Mycology 93: 65–153. https://doi.org/10.1016/j.simyco.2018.07.001
Wang, X.W., Han, P.J., Bai, F.Y., Lou, A., Bensch, K., Meijer, M., Kraak, B., Han, D.Y., Sun, B.D., Crous, P.W. & Houbraken, J. (2022) Taxonomy, phylogeny and identification of chaetomiaceae with emphasis on thermophilic species. Studies in Mycology 101: 121–243. https://doi.org/10.3114/sim.2022.101.03
White, T.J., Bruns, T., Lee, S. & Taylor, J. (1999) Amplification and direct sequencing of fungal ribosomal RNA genes for polygenetics. PCR Protocols. Academic Press, Inc, pp. 315–322. https://doi.org/10.1016/B978-0-12-372180-8.50042-1
Wijayawardene, N.N., Hyde, K.D., Al-Ani, L.K.T., Tedersoo, L., Haelewaters, D., Rajeshkumar, K.C., Zhao, R.L., Aptroot, A., Leontyev, D., V, Saxena, R.K., Tokarev, Y.S., Dai, D.Q., Letcher, P.M., Stephenson, S.L., Ertz, D., Lumbsch, H.T., Kukwa, M., Issi, I.V., Madrid, H., Phillips, A.J.L., Selbmann, L., Pfliegler, W.P., Horvath, E., Bensch, K., Kirk, P.M., Kolarikova, K., Raja, H.A., Radek, R., Papp, V, Dima, B., Ma, J., Malosso, E., Takamatsu, S., Rambold, G., Gannibal, P.B., Triebel, D., Gautam, A.K., Avasthi, S., Suetrong, S., Timdal, E., Fryar, S.C., Delgado, G., Reblova, M., Doilom, M., Dolatabadi, S., Pawlowska, J.Z., Humber, R.A., Kodsueb, R., Sanchez-Castro, I, Goto, B.T., Silva, D.K.A., de Souza, F.A., Oehl, F.R., da Silva, G.A., Silva, I.R., Blaszkowski, J., Jobim, K., Maia, L.C., Barbosa, F.R., Fiuza, P.O., Divakar, P.K., Shenoy, B.D., Castaneda-Ruiz, R.F., Somrithipol, S., Lateef, A.A., Karunarathna, S.C., Tibpromma, S., Mortimer, P.E., Wanasinghe, D.N., Phookamsak, R., Xu, J., Wang, Y., Tian, F., Alvarado, P., Li, D.W., Kusan, I, Matocec, N., Mesic, A., Tkalcec, Z., Maharachchikumbura, S.S.N., Papizadeh, M., Heredia, G., Wartchow, F., Bakhshi, M., Boehm, E., Youssef, N., Hustad, V.P., Lawrey, J.D., Santiago, A.L.C.M.A., Bezerra, J.D.P., Souza-Motta, C.M., Firmino, A.L., Tian, Q., Houbraken, J., Hongsanan, S., Tanaka, K., Dissanayake, A.J., Monteiro, J.S., Grossart, H.P., Suija, A., Weerakoon, G., Etayo, J., Tsurykau, A., Vazquez, V., Mungai, P., Damm, U., Li, Q.R., Zhang, H., Boonmee, S., Lu, Y.Z., Becerra, A.G., Kendrick, B., Brearley, F.Q., Motiejunaite, J., Sharma, B., Khare, R., Gaikwad, S., Wijesundara, D.S.A., Tang, L.Z., He, M.Q., Flakus, A., Rodriguez-Flakus, P., Zhurbenko, M.P., McKenzie, E.H.C., Stadler, M., Bhat, D.J., Liu, J.K., Raza, M., Jeewon, R., Nassonova, E.S., Prieto, M., Jayalal, R.G.U., Erdogdu, M., Yurkov, A., Schnittler, M., Shchepin, O.N., Novozhilov, Y.K., Silva-Filho, A.G.S., Gentekaki, E., Liu, P., Cavender, J.C., Kang, Y., Mohammad, S., Zhang, L.F., Xu, R.F., Li, Y.M., Dayarathne, M.C., Ekanayaka, A.H., Wen, T.C., Deng, C.Y., Pereira, O.L., Navathe, S., Hawksworth, D.L., Fan, X.L., Dissanayake, L.S., Kuhnert, E. & Thines, M. (2020) Outline of Fungi and fungus-like taxa. Mycosphere 11 (1): 1060–1456.