Abstract
A new species in Megasporaceae, Oxneriaria pakistanica is described and illustrated from Pakistan. A comparative morpho–anatomical study and ITS–based molecular analyses confirmed its position within the recently resurrected genus, Oxneriaria. The taxon has non-zonate thallus, epruinose, distinctly areolate, 0.5–1.8 mm wide areole, grey to whitish grey at upper surface, prothallus absent, 0.1–2 mm in diameter apothecia, taller hymenium 100–155 µm, deeper hypothecium 90–170 µm, broadly ellipsoid to sub-spherical ascospores 10–18 × 7–10 µm size and chemistry unknown substance detected. Its forms a separate branch in the phylogenetic tree and also distinct from the other known taxa of the genus. Oxneriaria pakistanica sp. nov appeared to be a sister specie to Oxneriaria rivulicola. A detailed comparison and a key to all taxa of Oxneriaria is also given.
References
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Hale, M.E. (1979) How to know the lichens, WC Brown Co..
Hall, T.A. (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic acids symposium series (Vol. 41, No. 41, pp. 95–98). [London]: Information Retrieval Ltd., c1979–c2000. https://doi.org/10.1556/034.59.2017.3-4.5
Nimis, P.L. (2016) ITALIC–the information system on Italian lichens. Versions 5.0 University of Trieste. Dept. of Biology. [http://dryades.units.it/italic]
Nordin, A., Björn, O.L. & Tibell, L. (2011) Two new Aspicilia species from Fennoscandia and Russia. The Lichenologist 43 (1): 27–37. https://doi.org/10.1017/S0024282910000629
Nordin, A., Savi?, S. & Tibell, L. (2010) Phylogeny and taxonomy of Aspicilia and Megasporaceae. Mycologia 102: 1339–1349. https://doi.org/10.3852/09-266
Orange, A., James, P.W. & White, F.J. (2010) Microchemical methods for the identification of lichens. British Lichen Society.
Paukov, A.G., Davydov, E.A., Nordin, A., Claude, R.O.U.X., ?enkarde?ler, A. Sohrabi, M., Vondrák, J., Frolov, I.V., Teptina, A.Y. & Shiryaeva, A.S. (2019) Three new species, new combinations and a key to known species of Lobothallia (Megasporaceae). The Lichenologist 51 (4): 301–322. https://doi.org/10.1017/S0024282919000264
Sambrook, J. & Russell, D.W. (2006) Rapid isolation of yeast DNA. Cold Spring Harbor Protocols 2006 (1). https://doi.org/10.1101/pdb.prot4039
Sohrabi, M., Rico, S.D.L.V.J., Halici, M.G., Shrestha, G. & Stenroos, S. (2013a) Teuvoa, a new lichen genus in Megasporaceae (Ascomycota: Pertusariales), including Teuvoa junipericola sp. nov. The Lichenologist 45: 347–360. https://doi.org/10.1017/S0024282913000108
Sohrabi, M., Stenroos, S., Myllys, L., Søchting, U., Ahti, T. & Hyvönen, J. (2013b) Phylogeny and taxonomy of the “manna lichens.” Mycological Progress 12: 231–269. https://doi.org/10.1007/s11557-012-0830-1
Tamura, K., Stecher, G., Peterson, D., Filipski, A. & Kumar, S. (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Molecular biology and evolution 30 (12): 2725–2729. https://doi.org/10.1093/molbev/mst197
Usman, M., Dyer, P.S. & Khalid, A.N. (2021) A novel arctic-alpine lichen from Deosai National Park, Gilgit Baltistan, Pakistan. The Bryologist 124 (4): 484–493. https://doi.org/10.1639/0007-2745-124.4.484
White, T.J.T., Lee, B.S.J.W.T. & Taylor, J. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR protocols: a guide to methods and applications 18 (1): 315–322. https://doi.org/10.1016/B978-0-12-372180-8.50042-1
Zakeri, Z., Divakar, P.K. & Otte, V. (2017) Taxonomy and phylogeny of Aspiciliella, a resurrected genus of Megasporaceae, including the new species A. portosantana. Herzogia 30: 166–176. https://doi.org/10.13158/heia.30.1.2017.166
Zulfiqar, R., Asghar, S., Habib, K. & Khalid, N. (2023) Two new species of the genus Oxneriaria (lichenized Ascomycetes: Megasporaceae) from Pakistan. Plant Systematics and Evolution 309. [Online] https://doi.org/10.1007/s00606-022-01836-w