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Issue: Vol. 566 No. 1: 22 September 2022
Type: Article
Published: 2022-09-22
DOI: 10.11646/phytotaxa.566.1.6
Page range: 105-120
Abstract views: 342
PDF downloaded: 4

Combination of morphological and molecular data support Pestalotiopsis eleutherococci (Sporocadaceae) as a new species

Internationally Cooperative Research Center of China for New Germplasm Breeding of Edible Mushroom, Jilin Agricultural University, Changchun 130118, China. College of Plant Protection, Jilin Agricultural University, Changchun 130118, China.
Internationally Cooperative Research Center of China for New Germplasm Breeding of Edible Mushroom, Jilin Agricultural University, Changchun 130118, China. College of Plant Protection, Jilin Agricultural University, Changchun 130118, China.
Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand. School of Science, Mae Fah Luang University, Chiang Rai, 57100, Thailand.
Internationally Cooperative Research Center of China for New Germplasm Breeding of Edible Mushroom, Jilin Agricultural University, Changchun 130118, China. College of Plant Protection, Jilin Agricultural University, Changchun 130118, China.
Internationally Cooperative Research Center of China for New Germplasm Breeding of Edible Mushroom, Jilin Agricultural University, Changchun 130118, China. Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand. School of Science, Mae Fah Luang University, Chiang Rai, 57100, Thailand. Jiaxing Key Laboratory for New Germplasm Breeding of Economic Mycology, Jiaxing 314000, China.
Internationally Cooperative Research Center of China for New Germplasm Breeding of Edible Mushroom, Jilin Agricultural University, Changchun 130118, China.
Internationally Cooperative Research Center of China for New Germplasm Breeding of Edible Mushroom, Jilin Agricultural University, Changchun 130118, China. College of Plant Protection, Jilin Agricultural University, Changchun 130118, China.
Internationally Cooperative Research Center of China for New Germplasm Breeding of Edible Mushroom, Jilin Agricultural University, Changchun 130118, China. College of Plant Protection, Jilin Agricultural University, Changchun 130118, China.
1 new species China Eleutherococcus phylogeny taxonomy Fungi

Abstract

Molecular barcoding and morphological characters were used to identify a new saprotrophic species in Pestalotiopsis, which was associated with senescent leaves of Eleutherococcus brachypus (Araliaceae) in Jilin Province, China. The matrix of the internal transcribed spacer (ITS) region, translation elongation factor 1-alpha (tef1-α), and β-tubulin (tub2) were used in the maximum likelihood (ML), maximum parsimony (MP) and Bayesian inference (BI) methods. The new collections formed a distinct clade with Pestalotiopsis lijiangensis. The new species differs from P. lijiangensis by its conidial length/width ratio. Detailed description and micrographs revealed that the species is unique in its olivaceous concolorous median cells and has significantly smaller conidia compared to other related species. The position of the apical appendages of Pestalotiopsis eleutherococci are distinct and are slightly shorter while the basal appendage is slightly longer compared to P. lijiangensis. Therefore, we introduce Pestalotiopsis eleutherococci as a novel species.

References

<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;"><span lang="en-GB">Akinsanmi, O.A., Nisa, S., Jeff-Ego, O.S., Shivas, R.G. & Drenth, A. (2017) Dry Flower Disease of Macadamia in Australia Caused by </span>Neopestalotiopsis macadamiae sp. nov. and Pestalotiopsis macadamiae sp. nov. Plant Disease 101 (1): 45–53. https://doi.org/10.1094/PDIS-05-16-0630-RE</span></span></span>

<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Ariyawansa, H.A. & Hyde, K.D. (2018) Additions to Pestalotiopsis in Taiwan. Mycosphere 9 (5): 999–1013. https://doi.org/10.5943/mycosphere/9/5/4</span></span></span>

<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Ariyawansa, H.A., Hyde, K.D., Jayasiri, S.C., Buyck, B., Chethana, K.W.T., Dai, D.Q., Dai, Y.C., Daranagama, D.A., Jayawardena, R.S., Lücking, R., Ghobad-Nejhad, M., Niskanen, T., Thambugala, K.M., Voigt, K., Zhao, R.L., Li, G.J., Doilom, M., Boonmee, S., Yang, Z.L., Cai, Q., Cui, Y.Y., Bahkali, A.H., Chen, J., Cui, B.K., Chen, J.J., Dayarathne, M.C., Dissanayake, A.J., Ekanayaka, A.H., Hashimoto, A., Hongsanan, S., Jones, E.B.G., Larsson, E., Li, W.J., Li, Q.R., Liu, J.K., Luo, Z.L., Maharachchikumbura, S.S.N., Mapook, A., McKenzie, E.H.C., Norphanphoun, C., Konta, S., Pang, K.L., Perera, R.H., Phookamsak, R., Phukhamsakda, C., Pinruan, U., Randrianjohany, E., Singtripop, C., Tanaka, K., Tian, C.M., Tibpromma, S., Abdel-Wahab, M.A., Wanasinghe, D.N., Wijayawardene, N.N., Zhang, J.F., Zhang, H., Abdel-Aziz, F.A., Wedin, M., Westberg, M., Ammirati, J.F., Bulgakov, T.S., Lima, D.X., Callaghan, T.M., Callac, P., Chang, C.H., Coca, L.F., Dal-Forno, M., Dollhofer, V., Fliegerová, K., Greiner, K., Griffith, G.W., Ho, H.M., Hofstetter, V., Jeewon, R., Kang, J.C., Wen, T.C., Kirk, P.M., Kytövuori, I., Lawrey, J.D., Xing, J., Li, H., Liu, Z.Y., Liu, X.Z., Liimatainen, K., Lumbsch, H.T., Matsumura, M., Moncada, B., Nuankaew, S., Parnmen, S., de Azevedo Santiago, A.L.C.M., Sommai, S., Song, Y., de Souza, C.A.F., de Souza-Motta, C.M., Su, H.Y., Suetrong, S., Wang, Y., Wei, S.F., Wen, T.C., Yuan, H.S., Zhou, L.W., Réblová, M., Fournier, J., Camporesi, E., Luangsa-ard, J.J., Tasanathai, K. & Khonsanit, A. (2015) Fungal diversity notes 111–252—taxonomic and phylogenetic contributions to fungal taxa. Fungal diversity 75 (1): 27–274. https://doi.org/10.1007/s13225-015-0346-5</span></span></span>

<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Barr, M.E. (1990) Prodromus to nonlichenized, pyrenomycetous members of class Hymenoascomycetes. Mycotaxon 39: 43–184.</span></span></span>

<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Barr, M.E. (1975) Pestalosphaeria, a new genus in the Amphisphaeriaceae. Mycologia 67: 187–194. https://doi.org/10.2307/3758246</span></span></span>

<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Chaiwan, N., Wanasinghe, D.N., Mapook, A., Jayawardena, R.S., Norphanphoun, C. & Hyde, K.D. (2020) Novel species of Pestalotiopsis fungi on Dracaena from Thailand. Mycology 11 (4): 306–315. https://doi.org/10.1080/21501203.2020.1801873.</span></span></span>

<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Chen, L.J., Li, H.Y., Jiao, W.W., Tao, M., Lv, C.Y., Zhao, M. & Wang, M. (2021) Genetic variation and demographic history analysis of Pestalotiopsis, Pseudopestalotiopsis, and Neopestalotiopsis fungi associated with tea (Camellia sinensis) inferred from the internal transcribed spacer region of the nuclear ribosomal DNA. Plant Pathology 70 (3): 699–711. https://doi.org/10.1111/ppa.13315</span></span></span>

<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Chen, Y.Y., Maharachchikumbura, S.S.N., Liu, J.K., Hyde, K.D., Nanayakkara, R.R., Zhu, G.S. & Liu, Z.Y. (2017) Fungi from Asian Karst formations I. Pestalotiopsis photinicola sp. nov., causing leaf spots of Photinia serrulata. Mycosphere 8 (1): 103–110. https://doi.org/10.5943/mycosphere/8/1/9</span></span></span>

<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Chethana, K.W., Manawasinghe, I.S., Hurdeal, V.G., Bhunjun, C.S., Appadoo, M.A., Gentekaki, E., Raspé, O., Promputtha, I. & Hyde, K.D. (2021) What are fungal species and how to delineate them? Fungal Diversity 109 (1): 1–25. https://doi.org/10.1007/s13225-021-00483-9</span></span></span>

<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Crous, P.W., Summerell, B.A., Swart, L., Denman, S., Taylor, J.E., Bezuidenhout, C.M., Palm, M.E., Marincowitz, S. & Groenewald, J.Z. (2011) Fungal pathogens of Proteaceae. Persoonia 27: 20–45. https://doi.org/10.3767/003158511X606239</span></span></span>

<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Crous, P.W., Wingfield, M.J., Burgess, T.I., Carnegie, A.J., Hardy, G.E.S.J., Smith, D., Summerell, B.A., Cano-Lira, J.F., Guarro, J., Houbraken, J., Lombard, L., Martín, M.P., Sandoval-Denis, M., Alexandrova, A.V., Barnes, C.W., Baseia, I.G., Bezerra, J.D.P., Guarnaccia, V., May, T.W., Hernández-Restrepo, M., Stchigel, A.M., Miller, A.N., Ordoñez, M.E., Abreu, V.P., Accioly, T., Agnello, C., Agustin Colmán, A., Albuquerque, C.C., Alfredo, D.S., Alvarado, P., Araújo-Magalhães, G.R., Arauzo, S., Atkinson, T., Barili, A., Barreto, R.W., Bezerra, J.L., Cabral, T.S., Camello Rodríguez, F., Cruz, R.H.S.F., Daniëls, P.P., da Silva, B.D.B., de Almeida, D.A.C., de Carvalho Júnior, A.A., Decock, C.A., Delgat, L., Denman, S., Dimitrov, R.A., Edwards, J., Fedosova, A.G., Ferreira, R.J., Firmino, A.L., Flores, J.A., García, D., Gené, J., Giraldo, A., Góis, J.S., Gomes, A.A.M., Gonçalves, C.M., Gouliamova, D.E., Groenewald, M., Guéorguiev, B.V., Guevara-Suarez, M., Gusmão, L.F.P., Hosaka, K., Hubka, V., Huhndorf, S.M., Jadan, M., Jurjevi?, Ž., Kraak, B., Ku?era, V., Kumar, T.K.A., Kušan, I., Lacerda, S.R., Lamlertthon, S., Lisboa, W.S., Loizides, M., Luangsa-Ard, J.J., Lysková, P., Mac Cormack, W.P., Macedo, D.M., Machado, A.R., Malysheva, E.F., Marinho, P., Mato?ec, N., Meijer, M., Meši?, A., Mongkolsamrit, S., Moreira, K.A., Morozova, O.V., Nair, K.U., Nakamura, N., Noisripoom, W., Olariaga, I., Oliveira, R.J.V., Paiva, L.M., Pawar, P., Pereira, O.L., Peterson, S.W., Prieto, M., Rodríguez-Andrade, E., Rojo De Blas, C., Roy, M., Santos, E.S., Sharma, R., Silva, G.A., Souza-Motta, C.M., Takeuchi-Kaneko, Y., Tanaka, C., Thakur, A., Smith, M.T., Tkal?ec, Z., Valenzuela-Lopez, N., van der Kleij, P., Verbeken, A., Viana, M.G., Wang, X.W. & Groenewald, J.Z. (2017) Fungal Planet description sheets: 625–715. Persoonia 39: 270–467. https://doi.org/10.3767/persoonia.2017.39.11</span></span></span>

<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Darriba, D., Taboada, G.L., Doallo, R. & Posada, D. (2012) jModelTest 2: more models, new heuristics and parallel computing. Nature Methods 9: 772–772. https://doi.org/10.1038/nmeth.2109</span></span></span>

<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Das, K., Lee, S.Y. & Jung, H.Y. (2020) Pestalotiopsis kaki sp. nov., a Novel Species Isolated from Persimmon Tree (Diospyros kaki) Bark in Korea. Mycobiology 49 (1): 54–60. https://doi.org/10.1080/12298093.2020.1852703</span></span></span>

<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Debbab, A., Aly, A.H. & Proksch, P. (2012) Endophytes and associated marine derived fungi ecological and chemical perspectives. Fungal Diversity 57: 45–83. https://doi.org/10.1007/s13225-012-0191-8</span></span></span>

<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Döring, M. (2007) Untersuchungen zur Vermehrung und Mykorrhiza von Eleutherococcus Maxim. (Araliaceae). Philipps Universität Marburg.</span></span></span>

<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Geng, K., Zhang, B., Song, Y., Hyde, K.D., Kang, J. & Wang, Y. (2013) A new species of Pestalotiopsis from leaf spots of Licuala grandis from Hainan, China. Phytotaxa 88 (3): 49–54. https://doi.org/10.11646/phytotaxa.88.3.2</span></span></span>

<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Gu, M., Hu, D., Han, B., Jiang, N. & Tian, C.M. (2021) Pestalotiopsis abietis sp. nov. from Abies fargesii in China. Phytotaxa 509 (1): 93–105. https://doi.org/10.11646/phytotaxa.509.1.4</span></span></span>

<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Glass, N.L. & Donaldson, G.C. (1995) Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes, Applied and Environmental Microbiology 61 (4): 1323–1330. https://doi.org/10.1128/aem.61.4.1323-1330.1995</span></span></span>

<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Hu, H.L., Jeewon, R., Zhou, D.Q., Zhou, T.X. & Hyde, K.D. (2007) Phylogenetic diversity of endophytic Pestalotiopsis species in Pinus armandii and Ribes spp.: Evidence from rDNA and ?–tubulin gene phylogenies. Fungal Diversity 24: 1–22.</span></span></span>

<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Hu, H.B., Zheng, X.D., Hu, H.S. & Li,Y. (2009) A New Neolignan Glycoside from the Roots of Acanthopanax brachypus. Acta Chimica Slovenica 56 (2).</span></span></span>

<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Huanaluek, N., Jayawardena, R.S., Maharachchikumbura, S.S.N. & Harishchandra, D. (2021) Additions to pestalotioid fungi in Thailand: Neopestalotiopsis hydeana sp. nov. and Pestalotiopsis hydei sp. nov. Phytotaxa 479 (1): 23–43. https://doi.org/10.11646/phytotaxa.479.1.2</span></span></span>

<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Heinig, U., Scholz, S. & Jennewein, S. (2013) Getting to the bottom of taxol biosynthesis by fungi, Fungal Diversity 60: 161–170. https://doi.org/10.1007/s13225-013-0228-7</span></span></span>

<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Hyde, K.D., Jeewon, R., Chen, Y.J., Bhunjun, C.S., Calabon, M.S., Jiang, H.B., Lin, C.G., Norphanphoun, C., Sysouphanthong, P., Pem, D., Tibpromma, S., Zhang, Q., Doilom, M., Jayawardena, R.S., Liu, J.K., Maharachchikumbura, S.S.N., Phukhamsakda, C., Phookamsak, R., Al-Sadi, A.M., Thongklang, N., Wang, Y., Gafforov, Y., Jones, E.B.G. & Lumyong, S. (2020) The numbers of fungi: is the descriptive curve flattening? Fungal Diversity 103: 219–271. https://doi.org/10.1007/s13225-020-00458-2</span></span></span>

<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Index Fungorum (2022) Index Fungorum. Available from: http://www.indexfungorum.org/names/names.asp (accessed 21 September 2022)</span></span></span>

<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Jayawardena, R.S., Hyde, K.D., de Farias, A.R., Bhunjun, C.S., Ferdinandez, H.S., Manamgoda, D.S., Udayanga, D., Herath, I.S., Thambugala, K.M., Manawasinghe, I.S. & Gajanayake, A.J. (2021) What is a species in fungal plant pathogens? Fungal Diversity 109 (1): 239–66. https://doi.org/10.1007/s13225-021-00484-8</span></span></span>

<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Joshi, S.D., Sanjay, R., Baby, U.I. & Mandal, A.K.A. (2009) Molecular characterization of Pestalotiopsis spp. associated with tea (Camellia sinensis) in southern India using RAPD and ISSR markers. Indian Journal of Biotechnology 8: 377–383.</span></span></span>

<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Kim, C.H. & Sun, B.Y. (2000) New taxa and combinations in Eleutherococcus (Araliaceae) from eastern Asia. Novon 10 (3): 209–214. https://doi.org/10.2307/3393101</span></span></span>

<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Ko, Ko.T., Stephenson, S.L., Bahkali, A.H. & Hyde, K.D. (2011a) From morphology to molecular biology: Can we use sequence data to identify fungal endophytes? Fungal Diversity 50: 113–120. https://doi.org/10.1007/s13225-011-0130-0</span></span></span>

<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Ko, Ko.T., McKenzie, E.H.C., Bahkali, A.H., To-Anun, C., Chukeatirote, E., Promputtha, I., Abd-Elsalam, K.A., Soytong, K., Wulandari, N.F., Sanoamuang, N., Jonglaekha, N., Kobsueb, R., Cheewangkoon, R., Wikee, S., Chamyuang, S. & Hyde, K.D. (2011b) The need for reinventory of Thai phytopathogens. Chiang Mai Journal of Science 38: 1–13.</span></span></span>

<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Katoh, K. & Standley, K. (2013) MAFFT Multiple Sequence Alignment Software Version 7: improvements in performance and usability. Molecular Biology 30: 772–780. https://doi.org/10.1093/molbev/mst010</span></span></span>

<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Lee, S., Crous, P.W. & Wingfield, M.J. (2006) Pestalotioid fungi from Restionaceae in the Cape Floral Kingdom. Studies in Mycology 55: 175–187. https://doi.org/10.3114/sim.55.1.175</span></span></span>

<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Larsson, A. (2014) AliView: a fast and lightweight alignment viewer and editor for large datasets. Bioinformatics 30 (22): 3276–3278. https://doi.org/10.1093/bioinformatics/btu531</span></span></span>

<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Liu, F., Hou, L., Raza, M. & Cai, L. (2017) Pestalotiopsis and allied genera from Camellia, with description of 11 new species from China. Scientific Reports 7 (1): 1–19. https://doi.org/10.1038/s41598-017-00972-5</span></span></span>

<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Liu, F., Bonthond, G., Groenewald, J.Z., Cai, L. & Crous, P.W. (2019) Sporocadaceae, a family of coelomycetous fungi with appendage-bearing conidia. Studies in Mycology 92. https://doi.org/10.1016/j.simyco.2018.11.001</span></span></span>

<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Li, L., Yang, Q. & Li, H. (2021) Morphology, phylogeny, and pathogenicity of Pestalotioid species on Camellia oleifera in China. Journal of Fungi 7 (12): 1080. https://doi.org/10.3390/jof7121080</span></span></span>

<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Ma, X.Y., Maharachchikumbura, S.S.N., Chen, B.W., Hyde, K.D., Eric, M., Putarak, C. & Kang, J.C. (2019) Endophytic pestalotiod taxa in Dendrobium orchids. Phytotaxa 419 (3): 268–286. https://doi.org/10.11646/phytotaxa.419.3.2</span></span></span>

<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Maharachchikumbura, S.S.N., Guo, L.D., Chukeatirote, E., Bahkali, A.H. & Hyde, K.D. (2011) Pestalotiopsis-morphology, phylogeny, biochemistry and diversity, Fungal Diversity 50: 167–187. https://doi.org/10.1007/s13225-011-0125-x</span></span></span>

<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Maharachchikumbura, S.S.N., Guo, L.D., Cai, L., Chukeatirote, E., Wu, W.P., Sun, X., Crous, P.W., Bhat, D.J., McKenzie, E.H.C., Bahkali, A.H. & Hyde, K.D. (2012) A multi-locus backbone tree for Pestalotiopsis, with a polyphasic characterization of 14 new species. Fungal Diversity 56: 95–129. https://doi.org/10.1007/s13225-012-0198-1</span></span></span>

<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Maharachchikumbura, S.S.N., Chukeatirote, E., Guo, L.D., Crous, P.W., McKenzie, E.H.C. & Hyde, K.D. (2013a) Pestalotiopsis species associated with Camellia sinensis (tea). Mycotaxon 123: 47–61. https://doi.org/10.5248/123.47</span></span></span>

<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Maharachchikumbura, S.S.N., Guo, L.D., Chukeatirote, E. & Hyde, K.D. (2013b) Improving the backbone tree for the genus Pestalotiopsis; addition of P. steyaertii and P. magna sp. nov. Mycological Progress 13: 617–624. https://doi.org/10.1007/s11557-013-0944-0</span></span></span>

<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Maharachchikumbura, S.S.N., Zhang, Y., Wang, Y. & Hyde, K.D. (2013c) Pestalotiopsis anacardiacearum sp. nov. (Amphisphaeriaceae) has an intricate relationship with Penicillaria jocosatrix the mango tip borer. Phytotaxa 99 (2): 49–57. https://doi.org/10.11646/phytotaxa.99.2.1</span></span></span>

<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Maharachchikumbura, S.S.N., Hyde, K.D., Jones, E.B.G., McKenzie, E.H.C., Huang, S.K., Abdel-Wahab, M.A., Daranagama, D.A., Dayarathne, M., D’souza, M.J., Goonasekara, I.D., Hongsanan, S., Jayawardena, R.S., Kirk, P.M., Konta, S., Liu, J.K., Liu, Z.Y., Norphanphoun, C., Pang, K.L., Perera, R.H., Senanayake, I.C., Shang, Q.J., Shenoy, B.D., Xiao, Y.P., Bahkali, A.H., Kang, J.C., Somrothipol, S., Suetrong, S., Wen, T.C. & Xu, J.C. (2015) Towards a natural classification and backbone tree for Sordariomycetes. Fungal Diversity 72 (1): 199–301. https://doi.org/10.1007/s13225-015-0331-z</span></span></span>

<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Maharachchikumbura, S.S.N., Guo, L.D., Liu, Z.Y. & Hyde, K.D. (2016) Pseudopestalotiopsis ignota and Ps. camelliae spp. nov. associated with grey blight disease of tea in China. Mycological Progress 15: 1–7. https://doi.org/10.1007/s11557-016-1162-3</span></span></span>

<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Maharachchikumbura, S.S.N., Hyde, K.D., Groenewald, J.Z., Xu, J. & Crous, P.W. (2014) Pestalotiopsis revisited. Studies in Mycology 79: 121–186. https://doi.org/10.1016/j.simyco.2014.09.005</span></span></span>

<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Monteiro, P.M., Gonçalves, M.F., Pinto, G., Silva, B., Martín-García, J., Diez, J.J. & Alves, A. (2021) Three novel species of fungi associated with pine species showing needle blight-like disease symptoms. European Journal of Plant Pathology 162 (1): 183–202. https://doi.org/10.1007/s10658-021-02395-5</span></span></span>

<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Nilsson, R.H., Larsson, K.H., Taylor, A.F., Bengtsson-Palme, J., Jeppesen, T.S., Schigel, D., Kennedy, P., Picard, K., Glöckner, F.O., Tedersoo, L. & Saar, I. (2019) The UNITE database for molecular identification of fungi: handling dark taxa and parallel taxonomic classifications. Nucleic Acids Research 47 (D1): D259–D264. https://doi.org/10.1093/nar/gky1022.</span></span></span>

<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Nozawa, S., Seto, Y. & Watanabe, K. (2019) First report of leaf blight caused by Pestalotiopsis chamaeropis and Neopestalotiopsis sp. in Japanese andromeda. Journal of General Plant Pathology 85 (6): 449–452. https://doi.org/10.1007/s10327-019-00868-4</span></span></span>

<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Nozawa, S., Yamaguchi, K., Yen, L.T.H., Van, Hop.D., Phay, N., Ando, K. & Watanabe, K. (2017) Identification of two new species and a sexual morph from the genus Pseudopestalotiopsis. Mycoscience 58 (5): 328–337. https://doi.org/10.1016/j.myc.2017.02.008</span></span></span>

<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Norphanphoun, C., Jayawardena, R.J., Chen, Y., Wen, T.C. & Hyde, K.D. (2019) Morphological and phylogenetic characterization of novel pestalotioid species associated with mangroves in Thailand. Mycosphere 10 (1): 531–578. https://doi.org/10.5943/mycosphere/10/1/9</span></span></span>

<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Nylander, J.A.A. (2004) MrModeltest 2.0. In Program Distributed by the Author; Uppsala University: Uppsala, Sweden.</span></span></span>

<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">O’Donnell, K. & Cigelnik, E. (1997) Two divergent intragenomic rDNA ITS2 types within a monophyletic lineage of the fungus Fusarium are no orthologous, Molecular Phylogenetics and Evolution 7 (1): 103–116. https://doi.org/10.1006/mpev.1996.0376</span></span></span>

<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Phukhamsakda, C., McKenzie, E., Phillips, A.J.L., Jones, E.B.G., Bhat, D.J., Marc, S., Bhunjun, C.S., Wanasinghe, D.N., Thongbai, B., Camporesi, E., Ertz, D., Jayawardena, R.S., Perera, R.H., Ekanayake, A.H., Tibpromma, S., Doilom, M., Xu, J. & Hyde, K.D. (2020) Microfungi associated with Clematis (Ranunculaceae) with an integrated approach to delimiting species boundaries. Fungal Diversity 102: 1–203. https://doi.org/10.1007/s13225-020-00448-4</span></span></span>

<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Rehner, S.A. & Buckley, E. (2005) A Beauveria phylogeny inferred from nuclear ITS and EF1-? sequences: evidence for cryptic diversification and links to Cordyceps teleomorphs. Mycologia 97 (1): 84–98. https://doi.org/10.3852/mycologia.97.1.84</span></span></span>

<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Rambaut, A. & Drummond, A. (2008) FigTree: Tree figure drawing tool, version 1. 2. 2. Institute of Evolutionary Biology University of Edinburgh.</span></span></span>

<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Ronquist, F. & Huelsenbeck, J.P. (2003) MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19: 1572–1574. https://doi.org/10.1093/bioinformatics/btg180</span></span></span>

<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Silva, N., Maharachchikumbura, S.S.N., Thambugala, K., Bhat, D.J. & Hyde, K.D. (2021a) Morpho-molecular taxonomic studies reveal a high number of endophytic fungi from Magnolia candolli and M. garrettii in China and Thailand. Mycosphere 12 (1): 163–237. https://doi.org/10.5943/mycosphere/12/1/3</span></span></span>

<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Silva, A.C., Diogo, E., Henriques, J., Ramos, A.P. & Bragana, H. (2021b) Pestalotiopsis pini sp. nov. an emerging pathogen on stone pine (Pinus pinea L.). Forests 11 (8): 805. https://doi.org/10.3390/f11080805</span></span></span>

<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Senanayake, I.C., Rathnayaka, A.R., Marasinghe, D.S., Calabon, M.S., Gentekaki, E., Lee, H.B., Hurdeal, V.G., Pem, D., Dissanayake, L.S., Wijesinghe, S.N., Bundhun, D., Nguyen, T.T., Goonasekara, I.D., Abeywickrama, P.D., Bhunjun, C.S., Jayawardena, R.S., Wanasinghe, D.N., Jeewon, R., Bhat, D.J. & Xiang, M.M. (2020a) Morphological approaches in studying fungi: Collection, examination, isolation, sporulation and preservation. Mycosphere 11: 2678–2754. https://doi.org/10.5943/mycosphere/11/1/20</span></span></span>

<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Senanayake, I.C., Lian, T.T., Mai, X.M., Jeewon, R., Maharachchikumbura, S.S.N., Hyde, K.D., Zeng, Y.J., Tian, S.L. & Xie, N. (2020b) New geographical records of Neopestalotiopsis and Pestalotiopsis species in Guangdong Province, China. Asian Journal of Mycology 3 (1): 512–533. https://doi.org/10.5943/ajom/3/1/19</span></span></span>

<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Song, Y., Geng, K., Hyde, K.D., Zhao, W., Wei, J., Kang, J. & Wang, Y. (2013) Two new species of Pestalotiopsis from Southern China. Phytotaxa 126: 22–32. https://doi.org/10.11646/phytotaxa.126.1.2</span></span></span>

<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Song, Y., Tangthirasunun, N., Maharachchikumbura, S.S.N., Jiang, Y., Xu, J., Hyde, K.D. & Wang, Y. (2014) Novel Pestalotiopsis species from Thailand point to the rich undiscovered diversity of this chemically creative genus. Cryptogamie Mycologie 35 (2): 119–138. https://doi.org/10.7872/crym.v35.iss2.2014.139</span></span></span>

<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Species Fungorum (2022) Species Fungorum. Available from: http://www.speciesfungorum.org/Names/Names.asp (accessed 21 September 2022)</span></span></span>

<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Stamatakis, A. (2006) RAxML-VI-HPC: Maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics 22: 2688–2690. https://doi.org/10.1093/bioinformatics/btl446</span></span></span>

<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Stamatakis, A., Hoover, P. & Rougemont, J. (2008) A rapid bootstrap algorithm for the RAxML web servers. Systematic Biology 57: 758–771. https://doi.org/10.1080/10635150802429642</span></span></span>

<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Stamatakis, A. (2014) RAxML version 8: A tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 30: 1312–1313. https://doi.org/10.1093/bioinformatics/btu033</span></span></span>

<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Strobel, G., Li, J.Y., Ford, E., Worapong, J., Baird, G.I. & Hess, W.M. (2000) Pestalotiopsis jesteri sp. nov. an endophyte from Fragraea bodenii, a common plant in the southern highlands of Papua New Guinea. Mycotaxon 76: 257–266.</span></span></span>

<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Tibpromma, S., Hyde, K.D., McKenzie, E.H., Bhat, D.J., Phillips, A.J., Wanasinghe, D.N., Samarakoon, M.C., Jayawardena, R.S., Dissanayake, A.J., Tennakoon, D.S., Doilom, M., Phookamsak, R., Tang, A.M., Xu, J., Mortimer, P.E., Promputtha, I., Maharachchikumbura, S.S.N., Khan, S. & Karunarathna, S.C. (2018) Fungal diversity notes 840–928: micro-fungi associated with Pandanaceae. Fungal Diversity 93 (4): 1–160. https://doi.org/10.1007/s13225-018-0408-6</span></span></span>

<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Vaidya, G., Lohman, D.J. & Meier, R. (2011) Sequence Matrix: concatenation software for the fast assembly of multi-gene datasets with character set and codon information. Cladistics 27 (2): 171–180.</span></span></span>

<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Vu, D., Groenewald, M., de Vries, M., Gehrmann, T., Stielow, B., Eberhardt, U., Al-Hatmi, A., Groenewald, J.Z., Cardinali, G., Houbraken, J., Boekhout, T., Crous, P.W., Robert, V. & Verkley, G.J.M. (2019) Large-scale generation and analysis of filamentous fungal DNA barcodes boosts coverage for kingdom fungi and reveals thresholds for fungal species and higher taxon delimitation. Studies in Mycology 92: 135–154. https://doi.org/10.1016/j.simyco.2018.05.001</span></span></span>

<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Watanabe, K., Nozawa, S., Hsiang, T. & Callan, B.E. (2018) The cup fungus Pestalopezia brunneopruinosa is Pestalotiopsis gibbosa and belongs to Sordariomycetes. Plos One 13 (6). https://doi.org/10.1371/journal.pone.0197025</span></span></span>

<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Watanabe, K., Motohashi, K. & Ono, Y. (2010) Description of Pestalotiopsis pallidotheae: a new species from Japan Mycoscience 51 (3): 182–188. https://doi.org/10.1007/s10267-009-0025-z</span></span></span>

<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Wei, J.G., Xu, T., Guo, L.D., Liu, A.R., Zhang, Y. & Pan, X.H. (2007) Endophytic Pestalotiopsis species associated with plants of Podocarpaceae, Theaceae and Taxaceae in southern China. Fungal Diversity 24: 55–74. https://doi.org/10.1016/j.funeco.2016.09.002</span></span></span>

<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">White, T., Bruns, T., Lee, S. & Taylor, J. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis, M.A., Gelfand, D.H., Sninsky, J.J. & White, T.J. (Eds.) PCR protocols: a guide to methods and applications. New York, Academic Press, pp. 315–322. https://doi.org/10.1016/B978-0-12-372180-8.50042-1</span></span></span>

<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Xu, J., Ebada, S.S. & Proksch, P. (2010) Pestalotiopsis a highly creative genus: Chemistry and bioactivity of secondary metabolites. Fungal Diversity 44: 15–31. https://doi.org/10.1007/s13225-010-0055-z</span></span></span>

<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Yook, C.S. (1997) Illustrated Book of Medicinal Plants. Seoul: Kyung-Won Media.</span></span></span>

<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Yun, Y.H., Ahn, G.R. & Kim, S.H. (2015) First report and characterization of Pestalotiopsis ellipsospora causing canker on Acanthopanax divaricatus. Mycobiology 43 (3): 366–370. https://doi.org/10.5941/MYCO.2015.43.3.366</span></span></span>

<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Zhou, Y.K., Li, F.P. & Hou, C.L. (2018) Pestalotiopsis lijiangensis sp. nov., a new endophytic fungus from Yunnan, China. Mycotaxon 133 (3): 513–522. https://doi.org/10.5248/133.513</span></span></span>

<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Zhang, Y.M., Maharachchikumbura, S.S.N., McKenzie, E.H.C. & Hyde, K.D. (2012a) A novel species of Pestalotiopsis causing leaf spots of Trachycarpus fortunei. Cryptogamie Mycologie 33: 1–8. https://dx.doi.org/10.7872/crym.v33.iss3.2012.311</span></span></span>

<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Zhang, Y.M., Maharachchikumbura, S.S.N., Tian, Q. & Hyde, K.D. (2013) Pestalotiopsis species on ornamental plants in Yunnan Province, China. Sydowia 65: 59–74.</span></span></span>

<p align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="font-size: small;">Zhang, Y.M., Maharachchikumbura, S.S.N., Wei, J.G., Mckenzie, E.H.C. & Hyde, K.D. (2012b) Pestalotiopsis camelliae, a new species associated with grey blight of Camellia japónica in China. Sydowia 64: 335–344.</span></span></span>

How to Cite

Tian, S., Xu, R., Bhunjun, C.S., Su, W., Hyde, K.D., Li, Y., Fu, Y. & Phukhamsakda, C. (2022)

Combination of morphological and molecular data support Pestalotiopsis eleutherococci (Sporocadaceae) as a new species

. Phytotaxa 566 (1): 105–120. https://doi.org/10.11646/phytotaxa.566.1.6