Cytotaxonomy of the endemic Karst and Danxia ferns in Adiantum (Pteridaceae)

AI-HUA WANG; XIAO-LING YU; XIAO-DONG MA; YUE-HONG YAN; FA-GUO WANG; HONG-FENG CHEN

doi:10.11646/phytotaxa.561.3.2

Issue: Vol. 561 No. 3: 12 September 2022

Type: Article

Published: 2022-09-12

DOI: 10.11646/phytotaxa.561.3.2

Page range: 243-255

Abstract views: 49

PDF downloaded: 53

**Cytotaxonomy of the endemic Karst and Danxia ferns in Adiantum (Pteridaceae)**

AI-HUA WANG⁺⁻
XIAO-LING YU⁺⁻
XIAO-DONG MA⁺⁻
YUE-HONG YAN⁺⁻
FA-GUO WANG⁺⁻
HONG-FENG CHEN⁺⁻

Key Laboratory of Plant Resources Conservation and Sustainable Utilization, Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China; Key Laboratory of Environment Change and Resources Use in Beibu Gulf, Ministry of Education, Nanning Normal University, Nanning 530001, China

Key Laboratory of Plant Resources Conservation and Sustainable Utilization, Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China

Institute of Ecological Conservation and Restoration, Chinese Academy of Forestry, Beijing 100091, China

Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization, the National Orchid Conservation Center of China and the Orchid Conservation & Research Center of Shenzhen, Shenzhen 518114, China

Key Laboratory of Plant Resources Conservation and Sustainable Utilization, Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China

cytotaxonomy chromosome numbers polyploidy cytotypes Adiantum Pteridophytes

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Abstract

Chromosome traits may differ between closely related ferns growing in different geological landscapes. Adiantum series Gravesiana (Pteridaceae) includes six species, of which, five species are exclusively distributed in Karst landforms, and one, Adiantum juxtapositum, grows on Danxia landform. Additionally, there are some populations or individuals whose morphology are between different species, which may be the results of polyploidization or hybridization. Here we studied chromosome numbers of species in series Gravesiana primarily using crosiers. Results are: A. longzhouensis and A. juxtapositum were diploids with 2n = 60 and 2n = 64, respectively; A. dentatum was deduced to be diploids with 2n = ca. 60; A. mariesii had two cytotypes, 2n = 60 (diploid) and 2n = ca. 120 (tetraploid), respectively; A. gravesii had four cytotypes including 2n = ca. 60 (diploid), ca. 120 (tetraploid), ca. 150 (pentaploid), and ca. 180 (hexaploid). All these different cytotypes suggest polyploidization and hybridization have occurred in A. gravesii and A. mariesii, so we treated these two species as two “complex group”. All chromosome numbers in this study are the first record.

References

Abraham, A., Ninan, C.A. & Mathew, P.M. (1962) Studies on the cytology and phylogeny of the pteridophytes-VII. Observation on one hundred species of South Indian ferns. The Journal of the Indian Botanical Society 41: 339–421.
Bir, S.S. & Verma, S.C. (2010) Chromosome atlas of the Indian pteridophytes (1951–2009). Bishen Singh Mahendra Pal Singh, Dehra Dun, 111–129.
de Peer, Y.V., Ashman, T.L., Soltis, P.S. & Soltis, D.E. (2021) Polyploidy: an evolutionary and ecological force in stressful times. The Plant Cell 33: 11–26. https://doi.org/10.1093/plcell/koaa015
Cao, J., Yuan, D. & Pan, G. (2003) Some soil features in Karst ecosystem. Advance in Earth Science 18: 37–44.
Cheng, X. & Zhang, S.Z. (2010) Index to chromosome numbers of Chinese pteridophyta (1969–2009). Journal of Fairylake Botanical Garden 9: 1–58.
Dong, S.Y. (2011) Taxonomic Studies on Asplenium sect. Thamnopteris (Aspleniaceae) I: Cytological Observations. American Fern Journal 101: 156–171. https://doi.org/10.1640/0002-8444-101.3.156
Glick, L. & Mayrose, I. (2014) ChromEvol: assessing the pattern of chromosome number evolution and the inference of polyploidy along a phylogeny. Molecular Biology and Evolution 31: 1914–1922. https://doi.org/10.1093/molbev/msu122
Guerra, M. (2008) Chromosome numbers in plant cytotaxonomy: concepts and implications. Cytogenetic and Genome Research 120: 339–350. https://doi.org/10.1159/000121083
He, Z., Yan, Y., Ma, Q. & Lu, Q. (2012) The bryophyte diversity of the Danxia landform in Hunan, China. Biodiversity Science 20: 522–526. https://doi.org/10.3724/SP.J.1003.2012.05216
Hsieh, T.H., Chang, Y.H., Chiou, W.L. & Huang, Y.M. (2008) Chromosome Numbers of Five Species of the Marattiaceae in Taiwan. Taiwan Journal of Forest Science 23: 377–381.
Irudayaraj, V. & Manickam, V.S. (1987) SOCGI plant chromosome number reports—V. Journal of Cytology and Genetics 22: 156–163.
Kato, M., Nakato, N., Cheng, X. & Iwatsuki, K. (1992) Cytotaxonomic Study of Ferns of Yunnan, Southwestern China. The Botanical Magazine, Tokyo 105: 105–124. https://doi.org/10.1007/BF02489407
Li, Y.B., Wang, S.J. & Li, R.L. (2004) Some soil features of karst ecosystem. Ecology and Environment 13: 434–438.
Liu, C.C., Liu, Y.G., Guo, K., Fan, D.Y., Li, G.W., Zheng, Y.R., Yu, L.F. & Yang, R. (2011) Effect of drought on pigments, osmotic adjustment and antioxidant enzymes in six woody plant species in karst habitats of southwestern China. Environment and Experimental Botany 71: 174–183. https://doi.org/10.1016/j.envexpbot.2010.11.012
Liu, Y., Liu, C., Rubinato, M., Guo, K., Zhou, J. & Cui, M. (2020) An Assessment of Soil’s Nutrient Deficiencies and Their Influence on the Restoration of Degraded Karst Vegetation in Southwest China. Forests 11: 797. https://doi.org/10.3390/f11080797
Liu, Y.G., Liu, C.C., Wang, S.J., Guo, K., Yang, J., Zhang, X.S. & Li, G.Q. (2013) Organic carbon storage in four ecosystem types in the karst region of southwestern China. PLoS ONE 8:e56443. https://doi.org/10.1371/journal.pone.0056443
Manickam, V.S. & Irudayaraj, V. (1988) Cytology of ferns of the Western Ghats (South India). Today and Tomorrow’s Printers and Publishers, New Delhi, pp. 81.
Manton, I., Sinha, B.M.B. & Vida, G. (1970) Cytotaxonomic studies in the Adiantum caudatum complex of Africa and Asia II. Autoploidy and alloploidy in African representaties of A. incisum. Botanical Journal of the Linnean Society 63: 1–21. [With 3 plates and 7 figures] https://doi.org/10.1111/j.1095-8339.1970.tb02299.x
Mehra, P.N. & Verma, S.C. (1960) Cytotaxonomic Observations on Some West Himalayan Pteridaceae. Caryologia 13: 619–650. https://doi.org/10.1080/00087114.1960.10797101
Nakato, N. & Kato, M. (2005) Cytogeography of the Adiantum pedatum complex (Pteridaceae, subfamily Adiantoideae). Acta Phytotaxonomica et Geobotanica 56: 85–96.
Paris, C.A. (1993) Adiantum. In: Flora of North America Edit. Comitt. (ed.) Flora of North America, North of Mexico. Vo1. 2. Pteridophytes and Gyrnnosperms, Oxford University Press, New York, pp. 125–130.
Paris, C.A. & Windham, M.D. (1988) A biosystematics investigation of the Adiantum pedatum complex in eastern North America. Systematic Botany 13: 240–255. https://doi.org/10.2307/2419103
Perwati, L.K. & dan Purnomo, S. (2002) cytotaxonomy of Adiantum genus in Merapi Mountain, Lawu Mountain and Dieng Plateu. Teknosains 15: 379–390
Pedro, J.S., Mario, R.M. & Claudio, P.R. (2006) Chromosome numbers of Chilean Pteridophytes: first contribution. Gayana Botanica 63: 115–118. https://doi.org/10.4067/S0717-66432006000100007
Qi, X.P. & Zhang, X.C. (2011) Cytotaxonomic study of three species of Lepisorus from China (Polypodiaceae). Guihaia 31: 181–183.
Roy, R.P. & Sinha, B.M.B. (1961) Meiotic Studies in the Genus Adiantum. Caryologia 14: 413–428. https://doi.org/10.1080/00087114.1961.10796044
Saggoo, M.I.S. & Kaur, M. (2016) Irregular Meiotic Behaviour in Maidenhair Fern Adiantum capillus-veneris L. from Northwest India. Cytologia 81: 77–82. https://doi.org/10.1508/cytologia.81.77
Shao, W. (2013) Cytological methods in fern by using embryo and young sporophyte. Acta Botanica Boreali-Occidentalia Sinica 33: 407–410.
Shing, K.H. & Wu, S.K. (1990) Adiantaceae. In: Ching, R.C. & Shing, K.H. (eds.) Flora Republicae Popularis Sinicae, 3 (1). Science Press, Beijing, 173–216.
Tindale, M.D. & Roy, S.K. (2002) A cytotaxonomic survey of the Pteridophyta of Australia. Australian systematic botany 15: 839–937. https://doi.org/10.1071/SB00034
Vijayakanth, P., Sathish, S.S., Rajkumar, D.S., Irudayaraj, V., Kavitha, R. & Mazumdar, J. (2018) Studies on the chromosome numbers of ferns from Kolli Hills, Eastern Ghats, Tamil Nadu, India. Caryologia 71: 380–396. https://doi.org/10.1080/00087114.2018.1482151
Wagner, W.H. Jr & Boydsten, K.E. (1978) A dwarf coastal variety of maidenhair fern Adiantum pedatum. Canadian Journal of Botany-revue canadienne de botanique 56: 1726–1729. https://doi.org/10.1139/b78-204
Walker, T.G. (1973) Evidence from cytology in the classification of ferns. Botanical Journal of the Linnean Society 67: 91–110.
Wang, A.H., Zhang, W.W., Li, D.L., Li, X.W., Yan, Y.H., Wang, F.G. & Xing, F.W. (2017) Revision of series Gravesiana (Adiantum L.) based on morphologic characters and phylogeny analyses. PLOS ONE 12: e0172729. https://doi.org/10.1371/journal.pone.0172729
Wang, K.L. & Zhang, C.H. (1999) Vulnerable characteristics of ecosystem, environmental problems and comprehensive strategies for ecological construction in the karst mountainous region of west Hunan. Journal of Mountain Research 17: 30–35.
Wang, R.X. (2011) Cytotaxonomic Study of 12 Species in the Polypodiaceae from Southern China. American Fern Journal 101: 307–316. https://doi.org/10.1640/0002-8444-101.4.307
Wang, R.X., Liu, L., Shao, W., Liu, J. & Deng, X.C. (2013) Cytotaxonomic study on two species of the Arthropteris from Guangxi, China. Guihaia 33: 723–726.
Wang, R.X., Lu, S.G. & Deng, X.C. (2007) Cytotaxonomic studies of the Chinese pteridophytes: A review. Acta Phytotaxonomica Sinica 45: 98–111. 
Wang, R.X., Shao, W., Bai, S.M. & Zhou, Z.P. (2018) Cytotaxonomic study of Plagiogyria (Plagiogyriaceae) from China. Flora 243: 53–57. https://doi.org/10.1016/j.flora.2018.04.004
Windham, M.D. & Yatskievych, G. (2003) Chromosome studies of Cheilanthoid ferns (Pteridaceae: Cheilanthoideae) from the western United States and Mexico. American Journal of Botany 90: 1788–1800. https://doi.org/10.3732/ajb.90.12.1788
Wu, Z.H. (1984) Cytology summary of Pteridophyte. Guihaia 4: 1–8.
Yan, Y.H., He, Z.X., Ma, Q.X., Zhou, X.L. & Lu, Q.Y. (2012) The pteridophyte diversity of the Danxia landform in Hunan Province, China. Biodiversity Science 20: 517–521. https://doi.org/10.3724/SP.J.1003.2012.05215
Zhang, G.M., Liao, W.B., Ding, M.Y., Lin, Y.X., Wu, Z.H., Zhang, X.C., Dong, S.Y., Prado, J., Gilbert, M.G., Yatskievych, G.Y., Ranker, T.A., Hooper, E.A., Alverson, E.R., Metzgar, J.S., Funston, M., Masuyama, S. & Kato, M. (2013) Pteridaceae. In: Wu, Z.Y., Raven, P.H. & Hong, D.Y. (eds.) Flora of China, Vols. 2–3. Science Press, Beijing; Missouri Botanical Garden Press, St. Louis, pp. 169–256.
Zhang, X.B., Liu, X.M. & Bai, X.Y. (2011) Application of a 137Cs fingerprinting technique for interpreting responses of sediment deposition of a karst depression to deforestation in the Guizhou Plateau, China. Science China Earth Science 54: 431–437. https://doi.org/10.1007/s11430-010-4105-x
Zhang, X.B. & Wang, K.L. (2009) Ponderation on the shortage of mineral nutrients in the soil-vegetation ecosystem in carbonate rock-distributed mountain regions in southwest China. Earth and Environment 37: 337–341.
Zheng, X.J. & Kuang, H.W. (2021) The uncanny workmanship of nature—Yadan, Danxia and Karst. Bulletin of Mineralogy, Petrology and Geochemistry 40: 992–997.
Zhou, L., Wang, X., Wang, Z., Zhang, X., Chen, C. & Liu, H. (2020) The challenge of soil loss control and vegetation restoration in the karst area of southwestern China. International Soil and Water Conservation Research 8: 26–34. https://doi.org/10.1016/j.iswcr.2019.12.001