Abstract
Two new species, Xylodon bambusinus and X. xinpingensis, are proposed based on morphological and molecular evidences. Both species share the annual growth habit, resupinate basidiomata and monomitic hyphal system with clamped, colorless generative hyphae, smooth, thin-walled basidiospores, but X. bambusinus is characterized by the smooth to tuberculate hymenial surface, presence of capitate and fusiform cystidia, broad ellipsoid basidiospores, while X. xinpingensis by the reticulate hymenophore with cream hymenial surface, and subglobose basidiospores (4.5–6 × 3.5–5 µm). Sequences of ITS and LSU nrRNA gene regions of the studied samples were generated, and phylogenetic analyses were performed with maximum likelihood, maximum parsimony and Bayesian inference methods. The phylogenetic analyses based on molecular data of ITS and ITS+nLSU sequences showed that X. bambusinus was sister to X. subclavatus, while X. xinpingensis grouped with X. astrocystidiatus and X. paradoxus. The nLSU dataset revealed that X. bambusinus grouped with X. asperus and X. brevisetus with lower supports, and that X. xinpingensis grouped with X. astrocystidiatus and X. paradoxus and then with X. rimosissimus without supports. Both morphological and molecular evidences confirmed the placement of two new species in Xylodon. Description and figures from the new species and a key to the known species of Xylodon from China are presented.
References
<p>Ariyawansa, H.A., Hyde, K.D., Jayasiri, S.C., Buyck, B., Chethana, K.T., Dai, D.Q., Dai, Y.C., Daranagama, D.A., Jayawardena, R.S., Lücking, R. & Ghobad-Nejhad, M. (2015) Fungal diversity notes 111–252 taxonomic and phylogenetic contributions to fungal taxa. Fungal Diversity 7: 27–274. https://doi.org/10.1007/s13225-015-0346-5<br>Bernicchia, A. & Gorjón, S.P. (2010) Fungi Europaei 12: Corticiaceae s.l. Edizioni Candusso, Lomazzo, pp. 1–1007.<br>Chen, C.C., Wu, S.H. & Chen, C.Y. (2017) Three new species of Hyphodontia s.l. (Basidiomycota) with poroid or raduloid hymenophore. Mycological Progress 16: 553–564. https://doi.org/10.1007/s11557-017-1286-0<br>Chen, C.C., Wu, S.H. & Chen, C.Y. (2018) Xylodon subflaviporus sp. nov. (Hymenochaetales, Basidiomycota) from East Asia. Mycoscience 59: 343–352. https://doi.org/10.1016/j.myc.2017.12.004<br>Chen, J.J., Zhou, L.W., Ji, X.H. & Zhao, C.L. (2016) Hyphodontia dimitica and H. subefibulata spp. nov. (Schizoporaceae, Hymenochaetales) from southern China based on morphological and molecular characters. Phytotaxa 269 (1): 1–13. https://doi.org/10.11646/phytotaxa.269.1.1<br>Chevallier, F.F. (1826) Flore Générale des Environs de Paris 1: 1–674.<br>Dai, Y.C. (2011) A revised checklist of corticioid and hydnoid fungi in China for 2010. Mycoscience 52: 69–79. https://doi.org/10.1007/S10267-010-0068-1<br>Dai, Y.C. (2012) Polypore diversity in China with an annotated checklist of Chinese polypores. Mycoscience 53: 49–80. https://doi.org/10.1007/s10267-011-0134-3<br>Donk, M.A. (1967) Notes on European polypores-II. Persoonia 5: 47–130.<br>Eriksson, J. (1958) Studies in the Heterobasidiomycetes and Homobasidiomycetes-Aphyllophorales of Muddus National Park in North Sweden. Symbolae Botanicae Upsalienses 16: 1–172.<br>Eriksson, J. & Ryvarden, L. (1976) The Corticiaceae of North Europe. Synopsis Fungorum 4: 547–886.<br>Felsenstein, J. (1985) Confidence intervals on phylogenetics: an approach using bootstrap. Evolution 39: 783–791. https://doi.org/10.1111/j.1558-5646.1985.tb00420.x<br>Fukami, T., Dickie, I.A., Paula Wilkie, P.J., Paulus, B.C., Park, D., Roberts, A., Buchanan, P.K. & Allen, R.B. (2010) Assembly history dictates ecosystem functioning: evidence from wood decomposer communities. Ecology Letters 13: 675–684. https://doi.org/10.1094/PHYTO.2001.91.9.905<br>Gilbertson, R.L. & Ryvarden, L. (1987) North American polypores 1-2. Fungiflora, Oslo. pp. 1–433.<br>Gray, S.F. (1821) A natural arrangement of British plants. London: Nabu Press 1: 649.<br>Guan, Q.X., Zhao, T.J. & Zhao, C.L. (2020) Morphological characters and phylogenetic analyses reveal two new species of Peniophorella from southern China. Mycological Progress 19: 397–404. https://doi.org/10.1007/s11557-020-01568-6<br>Hall, T.A. (1999) Bioedit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 41: 95–98.<br>Hibbett, D.S. & Binder, M. (2002) Evolution of complex fruiting-body morphologies in homobasidiomycetes. Proceedings of the Royal Society of London B: Biological Sciences 269: 1963–1969. https://doi.org/10.1098/rspb.2002.2123<br>Hjortstam, K. & Ryvarden, L. (1980) Studies in tropical Corticiaceae (Basidiomycetes) II. Mycotaxon 12: 168–184.<br>Jang, Y., Jang, S., Lee, J., Lee, H., Lim, Y.W., Kim, C. & Kim, J.J. (2016) Diversity of wood-inhabiting polyporoid and corticioid fungi in Odaesan National Park, Korea. Mycobiology 44: 217–236. https://doi.org/10.5941/MYCO.2016.44.4.217<br>Kan, Y.H., Gafforov, Y., Li, T. & Zhou, L.W. (2017a) Hyphodontia zhixiangii sp. nov. (Schizoporaceae, Basidiomycota) from Uzbekistan. Phytotaxa 299 (2): 273–279. https://doi.org/10.11646/phytotaxa.299.2.12<br>Kan, Y.H., Qin, W.M. & Zhou, L.W. (2017b) Hyphodontia mollissima sp. nov. (Schizoporaceae, Hymenochaetales) from Hainan, southern China. Mycoscience 58: 297–301. https://doi.org/10.1016/j.myc.2017.04.003<br>Larsson, K.H., Larsson, E. & Kõljalg, U. (2004) High phylogenetic diversity among corticioid homobasidiomycetes. Mycological Research 108: 983–1002. https://doi.org/10.5941/MYCO.2016.44.4.217<br>Larsson, K.H., Parmasto, E., Fischer, M., Langer, E., Nakasone, K.K. & Redhead, S.A. (2006) Hymenochaetales: a molecular phylogeny for the hymenochaetoid clade. Mycologia 98: 926–936. https://doi.org/10.3852/mycologia.98.6.926<br>Lee, I.S. & Langer, E. (2012) New records of Hyphodontia species from Taiwan. Nova Hedwigia 94: 239–244. https://doi.org/10.1127/0029-5035/2012/0094-0239<br>Liu, X.F., Shen, S. & Zhao, C.L. (2019) Morphological and molecular identification of a new species of Eichleriella (Auriculariales, Basidiomycota) in China. Phytotaxa 404 (6): 245–254. https://doi.org/10.11646/phytotaxa.404.6.3<br>Luo, K.Y., Ma, X. & Zhao, C.L. (2019) Neofavolus yunnanensis sp. nov. (Polyporales, Basidiomycota) from China: evidence from morphology and DNA sequence data. Phytotaxa 408 (2): 109–116. https://doi.org/10.11646/phytotaxa.408.2.3<br>Ma, X. & Zhao, C.L. (2019) Crepatura ellipsospora gen. et sp. nov. in Phanerochaetaceae (Polyporales, Basidiomycota) bearing a tuberculate hymenial surface. Mycological Progress 18: 785–793. https://doi.org/10.1007/s11557-019-01488-0<br>Miller, M.A., Holder, M.T., Vos, R., Midford, P.E., Liebowitz, T., Chan, L., Hoover, P. & Warnow, T. (2009) The CIPRES Portals. CIPRES. Available from: http://www.phylo.org/sub_sections/portal & http://www.webcitation.org/5imQlJeQa (accessed 4 August 2009)<br>Núñez, M. & Ryvarden, L. (2001) East Asian polypores 2. Synopsis Fungorum 14: 165–522.<br>Nylander, J.A.A. (2004) MrModeltest v2. Program distributed by the author. Evolutionary Biology Centre, Uppsala University.<br>Paulus, B., Hallenberg, N., Buchanan, P.K. & Chambers, G.K. (2000) A phylogenetic study of thegenus Schizopora (Basidiomycota) based on ITS DNA sequences. Mycological Research 104: 1155–1163. https://doi.org/10.1017/S0953756200002720<br>Peck, C.H. (1897) Report of the State Botanist (1896). Annual Report on the New York State Museum of Natural History 50: 77–159.<br>Petersen, J.H. (1996) Farvekort. The Danish Mycological Society’s colour-chart. Foreningen til Svampekundskabens Fremme , Greve. 6 pp.<br>Riebesehl, J. & Langer, E. (2017) Hyphodontia s.l. (Hymenochaetales, Basidiomycota): 35 new combinations and new keys to all 120 current species. Mycological Progress 16: 637–666. https://doi.org/10.1007/s11557-017-1299-8<br>Riebesehl, J., Yurchenko, E., Nakasone, K.K. & Langer, E. (2019) Phylogenetic and morphological studies in Xylodon (Hymenochaetales, Basidiomycota) with the addition of four new species. MycoKeys 47: 97–137. https://doi.org/10.3897/mycokeys.47.31130<br>Ronquist, F. & Huelsenbeck, J.P. (2003) MrBayes 3: bayesian phylogenetic inference under mixed models. Bioinformatics 19: 1572–1574. https://doi.org/10.1093/bioinformatics/btg180<br>Rosenthal, L.M., Larsson, K.H., Branco, S., Chung, J.A., Glassman, S.I., Liao, H.L., Peay, K.G., Smith, D.P., Talbot, J.M., Taylor, J.W., Vellinga, E.C., Vilgalys, R. & Bruns, T.D. (2017) Survey of corticioid fungi in North American pinaceous forests reveals hyperdiversity, underpopulated sequence databases, and species that are potentially ectomycorrhizal. Mycologia 109: 115–127. https://doi.org/10.1080/00275514.2017.1281677<br>Ryvarden, L. & Melo, I. (2014) Poroid fungi of Europe. Synopsis Fungorum 31: 1–455.<br>Shen, S., Ma, X., Xu, T.M. & Zhao, C.L. (2018) Phlebia ailaoshanensis sp. nov. (Polyporales, Basidiomycota) evidenced by morphological characters and phylogenetic analyses. Phytotaxa 373 (3): 184–196. https://doi.org/10.11646/phytotaxa.373.3.2<br>Shi, Z.W., Wang, X.W., Zhou, L.W. & Zhao, C.L. (2019) Xylodon kunmingensis sp. nov. (Hymenochaetales, Basidiomycota) from southern China. Mycoscience 60: 184–188. https://doi.org/10.1016/j.myc.2019.02.002<br>Stalpers, J.A. & Buchanan, P.K. (1991) Type studies of the species of Pellicularia and Peniophora described by G.H. Cunningham. New Zealand Journal of Botany 29: 331–340. https://doi.org/10.1080/0028825X.1991.10416611<br>Swofford, D.L. (2002) PAUP*: phylogenetic analysis using parsimony (*and other methods). Version 4.0b10. Sinauer Associates, Massachusetts.<br>Wang, M. & Chen, Y.Y. (2017) Phylogeny and taxonomy of the genus Hyphodontia (hymenochaetales, Basidiomycota) in China. Phytotaxa 309 (1): 45–54. https://doi.org/10.11646/phytotaxa.309.1.4<br>White, T.J., Bruns, T., Lee, S. & Taylor, J. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis, M.A., Gelfand, D.H., Sninsky, J.J. & White, T.J. (Eds.) PCR protocols: a guide to methods and applications. Academic Press, San Diego, pp. 315–322. https://doi.org/10.1016/B978-0-12-372180-8.50042-1<br>Wu, S.H. (1990) The Corticiaceae (Basidiomycetes) subfamilies Phlebioideae, Phanerochaetoideae and Hyphodermoideae in Taiwan. Annales Botanici Fennici 142: 1–123.<br>Wu, S.H. (2000) Studies on Schizopora flavipora s.l., with special emphasis on specimens from Taiwan. Mycotaxon 76: 51–66.<br>Wu, S.H. (2001) Three new species of Hyphodontia with poroid hymenial surface. Mycologia 93: 1019–1025. https://doi.org/10.2307/3761766<br>Wu, S.H. (2006) Hyphodontia tubuliformis, a new species from Taiwan. Mycotaxon 95: 185–188.<br>Xiong, H.X., Dai, Y.C. & Wu, S.H. (2009) Three new species of Hyphodontia from Taiwan. Mycological Progress 8: 165–169. https://doi.org/10.1007/s11557-009-0587-3<br>Xiong, H.X., Dai, Y.C. & Wu, S.H. (2010) Two new species of Hyphodontia from China. Mycologia 102: 918–922. https://doi.org/10.3852/09-139<br>Yombiyeni, P., Douanla-Meli, C., Amalfi, M. & Decock, C. (2011) Poroid Hymenochaetaceae from Guineo-Congolian rainforest: Phellinus gabonensis sp. nov. from Gabon – taxonomy and phylogenetic relationships. Mycological Progress 10: 351–362. https://doi.org/10.3852/14-298<br>Yurchenko, E. & Wu, S.H. (2013) Four new species of Hyphodontia (Xylodon s.s. Hjortstam & Ryvarden, Basidiomycota) from Taiwan. Nowa Hedwigia 96: 545–558. https://doi.org/10.1127/0029-5035/2013/0092<br>Yurchenko, E. & Wu, S.H. (2014) Three new species of Hyphodontia with peg-like hyphal aggregations. Mycological Progress 13: 533–545. https://doi.org/10.1007/s11557-013-0935-1<br>Zhao, C.L., Cui, B.K. & Dai, Y.C. (2014) Morphological and molecular identification of two new species of Hyphodontia (Schizoporaceae, Hymenochaetales) from southern China. Cryptogamie Mycologie 35: 87–97. https://doi.org/10.7872/crym.v35.iss1.2014.87<br>Zhao, C.L. & Wu, Z.Q. (2017) Ceriporiopsis kunmingensis sp. nov. (Polyporales, Basidiomycota) evidenced by morphological characters and phylogenetic analysis. Mycological Progress 16: 93–100. https://doi.org/10.1016/j.myc.2013.11.006<br>Zhao, Y.N., He, S.H., Nakasone, K.K., Wasantha, K.K.L., Chen, C.C., Liu, S.L., Ma, H.X. & Huang, M.R. (2021) Global phylogeny and taxonomy of the wood-decaying fungal genus Phlebiopsis (Polyporales, Basidiomycota). Frontiers in Microbiology 12: 622460. https://doi.org/10.3389/fmicb.2021.622460</p>