Abstract
Tuber mixtecorum is described as a new species from Mexico based on morphological and molecular evidence. This species belongs to the Maculatum clade and is characterized by its subtomentose to glabrous, whitish to pale brown peridium, dark brown gleba, ellipsoid reticulate-alveolate ascospores (15–33.9 × 11.3–23.3 µm), and the putative mycorrhizal association with Quercus castanea in Quercus-Pinus dry mixed forests.
References
Bonito, G.M., Gryganskyi, A.P., Trappe, J.M. & Vilgalys, R. (2010) A global meta analysis of Tuber ITS rDNA sequences: species diversity, host associations and long distance dispersal. Molecular Ecology 19 (22): 4994–5008. https://doi.org/10.1111/j.1365-294X.2010.04855.x
Bonito, G.M., Smith, M.E., Nowak, M., Healy, R.A., Guevara, G., Cázares, E., Kinoshita, A., Nouhra, E.R., Domínguez, L.S., Tedersoo, L., Murat, C., Wang, Y., Arroyo-Moreno, B., Pfister, D.H., Nara, K., Zambonelli, A., Trappe, J.M. & Vilgalys, R. (2013) Historical Biogeography and Diversification of Truffles in the Tuberaceae and Their Newly Identified Southern Hemisphere Sister Lineage. PLoS ONE 8 (1): e52765. https://doi.org/10.1371/journal.pone.0052765
Castellano, M.A., Trappe, J.M., Maser, Z. & Maser, S. (1986) Key to spores of the genera of hypogeous fungi of North America, with reference to animal mycophagy. Mad River Press, Eureka, 185 pp.
Castellano, M.A., Trappe, J.M. & Luoma, D.L. (2004) Sequestrate Fungi. In: Mueller, G.M., Bills, G.F. & Foster, M.S. (Eds.) Biodiversity of fungi. Inventory and monitoring methods. Elsevier Academic Press, San Diego, pp. 197–213. https://doi.org/10.1016/B978-012509551-8/50013-1
Cázares, E., García, J., Castillo, J. & Trappe, J.M. (1992) Hypogeous fungi from northern México. Mycologia 84: 341–359. https://doi.org/10.1080/00275514.1992.12026147
Darriba, D., Taboada, G.L., Doallo, R. & Posada, D. (2012) jModelTest 2: more models, new heuristics and parallel computing. Nature Methods 9: 772. https://doi.org/10.1038/nmeth.2109
Gardes, M. & Bruns, T.D. (1993) ITS primers with enhanced specificity for basidiomycetes—application to the identification of mycorrhizae and rusts. Molecular Ecology 2: 113–118. https://doi.org/10.1111/j.1365-294X.1993.tb00005.x
Gilkey, H.M. (1916) A revision of the Tuberales of California. University of California Publications in Botany 6: 275–356.
Gómez-Reyes, V.M., Vásquez-Marrufo, G., Ortega-Gómez, A.M. & Guevara-Guerrero, G. (2018) Ascomicetos hipogeos de la región occidental del Sistema Volcánico Transversal, México. Acta Botánica Mexicana 125: 37–48. https://doi.org/10.21829/abm125.2018.1327
Guevara, G., Bonito, G. & Cázares, E. (2013a) Revisión del género Tuber (Tuberaceae: Pezizales) de Mexico. Revista Mexicana de Biodiversidad 84: 39–49. https://doi.org/10.7550/rmb.31981
Guevara, G., Bonito, G., Trappe, J.M., Cázares, E., Williams, G., Healy, R.A., Schadt, C. & Vilgalys, R. (2013b) New North American truffles (Tuber spp.) and their ectomycorrhizal associations. Mycologia 105: 194–209. https://doi.org/10.3852/12–087
Guevara, G., Bonito, G., Cázares, E., Healy, R., Vilgalys, R. & Trappe, J.M. (2015) Novel Tuber spp. (Tuberaceae, Pezizales) in the Puberulum group from Mexico. Ascomycete 7: 367–374.
Guevara-Guerrero, G., Bonito, G., Smith, M.E., Healy, R., Grupe II, A.C., Cázares, E., Castellano, M.A. & Trappe, J.M. (2018) Tuber aztecorum sp. nov., a truffle species from Mexico belonging to the Maculatum clade (Tuberaceae, Pezizales). MycoKeys 30: 61–72. https://doi.org/10.3897/mycokeys.30.22887
Katoh, K., Misawa, K., Kuma, K. & Miyata, T. (2002) MAFFT: a novel method for rapid multiple sequence alignment based on fast Fourier transform. Nucleic Acids Research 30: 3059–3066. https://doi.org/10.1093/nar/gkf436
Kearse, M., Moir, R., Wilson, A., Stones-Havas, S., Cheung, M., Sturrock, S., Buxton, S., Cooper, A., Markowitz, S., Duran, C., Thierer, T., Ashton, B., Meintjes, P. & Drummon, A. (2012) Geneious Basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics 28: 1647–1649. https://doi.org/10.1093/bioinformatics/bts199
Kornerup, A. & Wanscher, H. (1978) Methuen handbook of colour. 3rd edition. Eyre, London, 227 pp.
Miller, M.A., Pfeiffer, W. & Schwartz, T. (2010) Creating the CIPRES Science Gateway for inference of large phylogenetic trees. In: Proceedings of the Gateway Computing Environments Workshop (GCE). 14 November 2010. New Orleans, LA, pp. 1–8. https://doi.org/10.1109/GCE.2010.5676129
Mullis, K. & Faloona, F.A. (1987) Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction. Methods in Enzymology 155: 335–350. https://doi.org/10.1016/0076-6879(87)55023-6
Murray, M.G. & Thompson, W.F. (1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acids Research 8 (19): 4321–4325. https://doi.org/10.1093/nar/8.19.4321
Payen, T., Murat, C. & Bonito, G. (2014) Truffle phylogenomics: New insights into truffle evolution and truffle life cycle. Advances in Botanical Research 70: 211–234. https://doi.org/10.1016/B978-0-12-397940-7.00007-0
Pegler, D.N., Spooner, B.M. & Young, T.W.K. (1993) British truffles. A revision of British hypogeous fungi. The Board of Trustees of the Royal Botanical Gardens, Kew, 215 pp.
Piña-Páez, C., Bonito, G., Guevara-Guerrero, G., Castellano, M.A., Garibay-Orijel, R., Trappe, J.M. & Peña-Rámirez, R. (2018) Description and distribution of Tuber incognitum sp. nov. and Tuber anniae in the Transmexican Volcanic Belt. MycoKeys 41: 17–27. https://doi.org/10.3897/mycokeys.41.28130
Ronquist, F., Teslenko, M., Van der Mark, P., Ayres, D.L., Darling, A., Höhna, S., Larget, B., Liu, L., Suchard, M.A. & Huelsenbeck, J.P. (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology 61: 539–542. https://doi.org/10.1093/sysbio/sys029
Trappe, J.M. & Guzman, G. (1971) Notes on Some Hypogeous Fungi from Mexico. Mycologia 63 (2): 317. https://doi.org/10.2307/3757764
Trappe, J.M., Molina, R., Luoma, D.L., Cázares, E., Pilz, D., Smith, J.E., Castellano, M.A., Miller, L. & Trappe, J.M. (2009) Diversity, ecology and conservation of the truffle fungi in forests of the pacific northwest. US Dept. of Agriculture, Forest Service General Technical Report, PNW-GTR-772, Portland 194 pp. https://doi.org/10.2737/PNW-GTR-772
Vittadini, C. (1831) Monographia tuberacearum. Mediolani, 88 pp.
White, T.J., Bruns, T.D., Lee, S. & Taylor, J.W. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis, M.A., Gelfand, D.H., Sninsky, J. & White, T.J. (Eds.) PCR protocols: a guide to methods and applications. Academic, San Diego, pp. 315–322.Wiggers, F.H. (1780) Primitiae florae Holsaticae. Kiliae, 112 pp. https://doi.org/10.1016/B978-0-12-372180-8.50042-1
Bonito, G.M., Smith, M.E., Nowak, M., Healy, R.A., Guevara, G., Cázares, E., Kinoshita, A., Nouhra, E.R., Domínguez, L.S., Tedersoo, L., Murat, C., Wang, Y., Arroyo-Moreno, B., Pfister, D.H., Nara, K., Zambonelli, A., Trappe, J.M. & Vilgalys, R. (2013) Historical Biogeography and Diversification of Truffles in the Tuberaceae and Their Newly Identified Southern Hemisphere Sister Lineage. PLoS ONE 8 (1): e52765. https://doi.org/10.1371/journal.pone.0052765
Castellano, M.A., Trappe, J.M., Maser, Z. & Maser, S. (1986) Key to spores of the genera of hypogeous fungi of North America, with reference to animal mycophagy. Mad River Press, Eureka, 185 pp.
Castellano, M.A., Trappe, J.M. & Luoma, D.L. (2004) Sequestrate Fungi. In: Mueller, G.M., Bills, G.F. & Foster, M.S. (Eds.) Biodiversity of fungi. Inventory and monitoring methods. Elsevier Academic Press, San Diego, pp. 197–213. https://doi.org/10.1016/B978-012509551-8/50013-1
Cázares, E., García, J., Castillo, J. & Trappe, J.M. (1992) Hypogeous fungi from northern México. Mycologia 84: 341–359. https://doi.org/10.1080/00275514.1992.12026147
Darriba, D., Taboada, G.L., Doallo, R. & Posada, D. (2012) jModelTest 2: more models, new heuristics and parallel computing. Nature Methods 9: 772. https://doi.org/10.1038/nmeth.2109
Gardes, M. & Bruns, T.D. (1993) ITS primers with enhanced specificity for basidiomycetes—application to the identification of mycorrhizae and rusts. Molecular Ecology 2: 113–118. https://doi.org/10.1111/j.1365-294X.1993.tb00005.x
Gilkey, H.M. (1916) A revision of the Tuberales of California. University of California Publications in Botany 6: 275–356.
Gómez-Reyes, V.M., Vásquez-Marrufo, G., Ortega-Gómez, A.M. & Guevara-Guerrero, G. (2018) Ascomicetos hipogeos de la región occidental del Sistema Volcánico Transversal, México. Acta Botánica Mexicana 125: 37–48. https://doi.org/10.21829/abm125.2018.1327
Guevara, G., Bonito, G. & Cázares, E. (2013a) Revisión del género Tuber (Tuberaceae: Pezizales) de Mexico. Revista Mexicana de Biodiversidad 84: 39–49. https://doi.org/10.7550/rmb.31981
Guevara, G., Bonito, G., Trappe, J.M., Cázares, E., Williams, G., Healy, R.A., Schadt, C. & Vilgalys, R. (2013b) New North American truffles (Tuber spp.) and their ectomycorrhizal associations. Mycologia 105: 194–209. https://doi.org/10.3852/12–087
Guevara, G., Bonito, G., Cázares, E., Healy, R., Vilgalys, R. & Trappe, J.M. (2015) Novel Tuber spp. (Tuberaceae, Pezizales) in the Puberulum group from Mexico. Ascomycete 7: 367–374.
Guevara-Guerrero, G., Bonito, G., Smith, M.E., Healy, R., Grupe II, A.C., Cázares, E., Castellano, M.A. & Trappe, J.M. (2018) Tuber aztecorum sp. nov., a truffle species from Mexico belonging to the Maculatum clade (Tuberaceae, Pezizales). MycoKeys 30: 61–72. https://doi.org/10.3897/mycokeys.30.22887
Katoh, K., Misawa, K., Kuma, K. & Miyata, T. (2002) MAFFT: a novel method for rapid multiple sequence alignment based on fast Fourier transform. Nucleic Acids Research 30: 3059–3066. https://doi.org/10.1093/nar/gkf436
Kearse, M., Moir, R., Wilson, A., Stones-Havas, S., Cheung, M., Sturrock, S., Buxton, S., Cooper, A., Markowitz, S., Duran, C., Thierer, T., Ashton, B., Meintjes, P. & Drummon, A. (2012) Geneious Basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics 28: 1647–1649. https://doi.org/10.1093/bioinformatics/bts199
Kornerup, A. & Wanscher, H. (1978) Methuen handbook of colour. 3rd edition. Eyre, London, 227 pp.
Miller, M.A., Pfeiffer, W. & Schwartz, T. (2010) Creating the CIPRES Science Gateway for inference of large phylogenetic trees. In: Proceedings of the Gateway Computing Environments Workshop (GCE). 14 November 2010. New Orleans, LA, pp. 1–8. https://doi.org/10.1109/GCE.2010.5676129
Mullis, K. & Faloona, F.A. (1987) Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction. Methods in Enzymology 155: 335–350. https://doi.org/10.1016/0076-6879(87)55023-6
Murray, M.G. & Thompson, W.F. (1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acids Research 8 (19): 4321–4325. https://doi.org/10.1093/nar/8.19.4321
Payen, T., Murat, C. & Bonito, G. (2014) Truffle phylogenomics: New insights into truffle evolution and truffle life cycle. Advances in Botanical Research 70: 211–234. https://doi.org/10.1016/B978-0-12-397940-7.00007-0
Pegler, D.N., Spooner, B.M. & Young, T.W.K. (1993) British truffles. A revision of British hypogeous fungi. The Board of Trustees of the Royal Botanical Gardens, Kew, 215 pp.
Piña-Páez, C., Bonito, G., Guevara-Guerrero, G., Castellano, M.A., Garibay-Orijel, R., Trappe, J.M. & Peña-Rámirez, R. (2018) Description and distribution of Tuber incognitum sp. nov. and Tuber anniae in the Transmexican Volcanic Belt. MycoKeys 41: 17–27. https://doi.org/10.3897/mycokeys.41.28130
Ronquist, F., Teslenko, M., Van der Mark, P., Ayres, D.L., Darling, A., Höhna, S., Larget, B., Liu, L., Suchard, M.A. & Huelsenbeck, J.P. (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology 61: 539–542. https://doi.org/10.1093/sysbio/sys029
Trappe, J.M. & Guzman, G. (1971) Notes on Some Hypogeous Fungi from Mexico. Mycologia 63 (2): 317. https://doi.org/10.2307/3757764
Trappe, J.M., Molina, R., Luoma, D.L., Cázares, E., Pilz, D., Smith, J.E., Castellano, M.A., Miller, L. & Trappe, J.M. (2009) Diversity, ecology and conservation of the truffle fungi in forests of the pacific northwest. US Dept. of Agriculture, Forest Service General Technical Report, PNW-GTR-772, Portland 194 pp. https://doi.org/10.2737/PNW-GTR-772
Vittadini, C. (1831) Monographia tuberacearum. Mediolani, 88 pp.
White, T.J., Bruns, T.D., Lee, S. & Taylor, J.W. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis, M.A., Gelfand, D.H., Sninsky, J. & White, T.J. (Eds.) PCR protocols: a guide to methods and applications. Academic, San Diego, pp. 315–322.Wiggers, F.H. (1780) Primitiae florae Holsaticae. Kiliae, 112 pp. https://doi.org/10.1016/B978-0-12-372180-8.50042-1