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Issue: Vol. 759 No. 3: 29 May 2026
Type: Article
Published: 2026-05-29
DOI: 10.11646/phytotaxa.759.3.3
Page range: 227-244
Abstract views: 0
PDF downloaded: 0

Calocybe kinabaluensis (Lyophyllaceae, Agaricales), a new species from Kinabalu UNESCO Global Geopark, Sabah, Northern Borneo, Malaysia

Institute for Tropical Biology and Conservation; Universiti Malaysia Sabah; Jalan UMS; 88400 Kota Kinabalu; Sabah; Malaysia
Department of Life Sciences and Systems Biology; University of Torino; Viale P.A. Mattioli 25; I-10125; Torino; Italy
Faculty of Natural Science and Sustainability; University College Sabah Foundation; Jalan Sanzac; Sembulan; 88100 Kota Kinabalu; Sabah; Malaysia
Sabah Parks; P.O.Box 10626; 88806 Kota Kinabalu; Sabah; Malaysia
Institute for Tropical Biology and Conservation; Universiti Malaysia Sabah; Jalan UMS; 88400 Kota Kinabalu; Sabah; Malaysia
Institute for Tropical Biology and Conservation; Universiti Malaysia Sabah; Jalan UMS; 88400 Kota Kinabalu; Sabah; Malaysia
Fungi Agaricales Basidiomycota Kinabalu Park Phylogeny Rugosomyces Taxonomy

Abstract

Calocybe kinabaluensis sp. nov. was collected and described from Kinabalu UNESCO Global Geopark in Sabah, Malaysia. Morphological and phylogenetic evidence were used to delimit the collections as a new species. The species is characterized by its small to medium-sized basidiomata, deep blue to indigo-blue pileus with an irregularly lobed and undulate margin, and whitish to pale cream adnate to decurrent lamellae. Microscopically, it is distinguished by ovoid to broadly ellipsoid basidiospores, a cutis-type pileipellis, and the presence of cheilocystidia. Phylogenetic analyses of concatenated ITS and LSU sequences and ITS-only sequences distinguish this species from the molecularly closely related taxa, including C. cyanella and C. longisterigma. A detailed description and illustrations of the new species as well as comparative notes with other blue-capped taxa are provided. This research represents the first description of a new Calocybe species from Sabah, Malaysia, expanding current knowledge of the genus and highlighting the region as a significant area for future fungal diversity investigations.

References

  1. Asif, M., Saba, M., Raza, M. & Vellinga, E.C. (2024) Molecular insights into fungal diversity reveal three novel species of Leucocoprinus from southern Punjab, Pakistan. Mycologia 116 (4): 601–620. https://doi.org/10.1080/00275514.2024.2351769
  2. Baroni, T.J., Legon, N.W., Vilgalys, R. & Lodge, D.J. (1999) Calocybe cyanea: A rare and beautiful agaric is discovered in Puerto Rico. Mycologist 13 (1): 7–10. https://doi.org/10.1016/S0269-915X(99)80065-2
  3. Bellanger, J.M., Moreau, P.A., Corriol, G., Bidaud, A., Chalange, R., Dudova, Z. & Richard, F. (2015) Plunging hands into the mushroom jar: A phylogenetic framework for Lyophyllaceae (Agaricales, Basidiomycota). Genetica 143 (2): 169–194. https://doi.org/10.1007/s10709-015-9823-8
  4. Bessette, A.E., Bessette, A.R. & Lewis, D.P. (2019) Mushrooms of the Gulf Coast States: A field guide to Texas, Louisiana, Mississippi, Alabama, and Florida. University of Texas Press, Austin, 400 pp. https://doi.org/10.7560/318157
  5. Bon, M. (1991) Les genres Echinoderma (Locq. ex Bon) st. nov. et Rugosomyces Raithelhuber ss. lato. Documents Mycologiques 21 (82): 61–66.
  6. Bon, M. (1999) Flore mycologique d’Europe. Les collybio-marasmioïdes et ressemblants. Documents Mycologiques. Mémoire hors série n° 5, Amiens, 171 pp.
  7. Corner, E.J.H. (1962) The Royal Society expedition to North Borneo 1961. Empire Forestry Review 1962: 224–233.
  8. Corner, E.J.H. (1965) Mount Kinabalu East. Sabah Society Journal 4: 170–187.
  9. Corner, E.J.H. (1970) Phylloporus Quél. and Paxillus Fr. in Malaya and Borneo. Nova Hedwigia 20: 793–822.
  10. Corner, E.J.H. (1976) On the clavarioid Ramaria stricta (Fr.) Quél. in Borneo. Persoonia 9: 149–150.
  11. Corner, E.J.H. (1996) The agaric genera Marasmius, Chaetocalathus, Crinipellis, Heimiomyces, Resupinatus, Xerula and Xerulina in Malesia. Beihefte zur Nova Hedwigia 111: 1–175.
  12. Dai, Y.C., Zhou, L.W., Yang, Z.L., Wen, H.A., Bau, T. & Li, T.H. (2010) A revised checklist of edible fungi in China.
  13. Deng, X., Li, M., Dai, Y., Zhu, X., Yan, X., Wei, Z. & Yuan, Y. (2024) The diversity of macrofungi in the forests of Ningxia, western China. Diversity 16 (12): 725. https://doi.org/10.3390/d16120725
  14. Donk, M.A. (1962) The generic names proposed for the Agaricaceae. Beihefte zur Nova Hedwigia 5: 1–320.
  15. Dousin, O., Aralas, S. & Denis, J.I. (2024) Geopark development and community well-being: The case of Kinabalu UNESCO Global Geopark, Sabah, Malaysian Borneo. International Journal of Geoheritage and Parks 12 (3): 384–395. https://doi.org/10.1016/j.ijgeop.2024.06.002
  16. Darriba, D., Taboada, G.L., Doallo, R. & Posada, D. (2012) jModelTest 2: more models, new heuristics and high-performance computing. Nature Methods 9 (8): 772. https://doi.org/10.1038/nmeth.2109
  17. Gardes, M. & Bruns, T.D. (1993) ITS primers with enhanced specificity for basidiomycetes—application to the identification of mycorrhizae and rusts. Molecular Ecology 2 (2): 113–118. https://doi.org/10.1111/j.1365-294X.1993.tb00005.x
  18. Garnica, S., Weiss, M., Walther, G. & Oberwinkler, F. (2007) Reconstructing the evolution of agarics from nuclear gene sequences and basidiospore ultrastructure. Mycological Research 111 (9): 1019–1029. https://doi.org/10.1016/j.mycres.2007.03.019
  19. Goh, H.C., Tan, W.H., Rosilawati, Z., Mariney, M.Y. & Adeline, T.H. (2013) Tourism development and visitor motivation in a world heritage site: The case of Kinabalu Park. Malaysian Journal of Tropical Geography 38 (1–2): 47–56.
  20. Hofstetter, V., Clémençon, H., Vilgalys, R. & Moncalvo, J.-M. (2002) Phylogenetic analyses of the Lyophylleae (Agaricales, Basidiomycota) based on nuclear and mitochondrial rDNA sequences. Mycological Research 106 (9): 1043–1059. https://doi.org/10.1017/S095375620200641X
  21. Hofstetter, V., Redhead, S.A., Kauff, F., Moncalvo, J.M., Matheny, P.B. & Vilgalys, R. (2014) Taxonomic revision and examination of ecological transitions of the Lyophyllaceae (Basidiomycota, Agaricales) based on a multigene phylogeny. Cryptogamie, Mycologie 35 (4): 399–425. https://doi.org/10.7872/crym.v35.iss4.2014.399
  22. Hu, W., He, Y., Yang, Z., Meng, Q. & Fu, S. (2024) Chemical constitutes from mycelia of Laetiporus versisporus (Agaricomycetes). International Journal of Medicinal Mushrooms 26 (8): 75–83. https://doi.org/10.1615/IntJMedMushrooms.2024054165
  23. Justine, V.T., Liew, T.S., Alphonsus, A., Kasmin, J., Mujih, H., Repin, R. & Suleiman, M. (2025) Species richness and distribution of alien plant species in Kinabalu Park, Malaysia. Folia Geobotanica 59 (3): 257–287. https://doi.org/10.1007/s12224-025-09463-2
  24. Kalamees, K. (1992) Tricholomella, a new genus, with the distribution data of Tricholomella constrictum, comb. nov. in east Europe and Asia. Persoonia 14 (4): 445–447.
  25. Kalamees, K. (2004) Palearctic Lyophyllaceae (Tricholomatales) in northern and eastern Europe and Asia. Estonian Agricultural University, Institute of Zoology and Botany.
  26. Kalyaanamoorthy, S., Minh, B.Q., Wong, T.K.F., von Haeseler, A. & Jermiin, L.S. (2017) ModelFinder: Fast model selection for accurate phylogenetic estimates. Nature Methods 14 (6): 587–589. https://doi.org/10.1038/nmeth.4285
  27. Khalkho, S., Koreti, D., Kosre, A., Jadhav, S.K. & Chandrawanshi, N.K. (2021) Review on production technique and nutritional status of Calocybe indica. NewBioWorld 3 (1): 1–7. https://doi.org/10.52228/NBW-JAAB.2021-3-1-2
  28. Kornerup, A. & Wanscher, J.H. (1978) Methuen handbook of colour. Methuen Publishing, London.
  29. Kühner, R. & Romagnesi, H. (1953) Flore analytique des champignons supérieurs. Masson, Paris.
  30. Kühner, R. (1938) Utilisation du carmin acétique dans la classification des agarics leucosporés. Bulletin Mensuel de la Societe Linneenne de Lyon 7 (7): 204–211 Publications de la Société Linnéenne de Lyon 7 (7): 204–211. https://doi.org/10.3406/linly.1938.9364
  31. Larsson, A. (2014) AliView: A fast and lightweight alignment viewer and editor for large datasets. Bioinformatics 30 (22): 3276–3278. https://doi.org/10.1093/bioinformatics/btu531
  32. Li, J.J., Wu, S.Y., Yu, X.D., Zhang, S.B. & Cao, D.X. (2017) Three new species of Calocybe (Agaricales, Basidiomycota) from northeastern China are supported by morphological and molecular data. Mycologia 109 (1): 55–63. https://doi.org/10.1080/00275514.2017.1286570
  33. Li, T., Huang, M., Mao, N., Zhang, Y. & Fan, L. (2025) Species diversity of Calocybe (Agaricales, Lyophyllaceae) from Shanxi Province of northern China. Diversity 17 (9): 619. https://doi.org/10.3390/d17090619
  34. Liu, Q., Liu, S., Li, J., Zhang, J., Zhou, F., Luo, X. & Sun, D. (2025) High resistance and yield: a new cultivar ‘ZJLZS002’ of Lyophyllum decastes suitable for industrial cultivation. Agriculture 15 (10): 1045. https://doi.org/10.3390/agriculture15101045
  35. Lu, J.R., Lei, L., Yu, F.M., Zhu, X.T., Liang, J.F., Jia, L.K., Mu, L.H., Zhang, J.M., Wang, G.S., Zhang, Y., Hyde, K.D., Yang, Z.L. & Zhao, Q. (2025) Diversity of macrofungi in southeast Xizang, China 2. Mycology 17 (1). https://doi.org/10.1080/21501203.2025.2525864
  36. Ma, A., Hu, J.J., Chen, Y.Q., Wang, X., Tuo, Y.L., Yue, L. & Li, Y. (2024) Multiple evidence reveals two new species and new distributions of Calocybe species from northeastern China. MycoKeys 103: 37–52. https://doi.org/10.3897/mycokeys.103.116605
  37. Mao, X.L. (2000) The macrofungi in China. Henan Science and Technology Press, Zhengzhou.
  38. Matheny, P.B., Curtis, J.M., Hofstetter, V., Aime, M.C., Moncalvo, J.M., Ge, Z.W. & Hibbett, D.S. (2006) Major clades of Agaricales: a multilocus phylogenetic overview. Mycologia 98 (6): 982–995. https://doi.org/10.1080/15572536.2006.11832627
  39. Moncalvo, J.-M., Vilgalys, R., Redhead, S.A., Johnson, J.E., James, T.Y., Aime, M.C., Hofstetter, V., Verduin, S.J.W., Larsson, E., Baroni, T.J., Thorn, R.G., Jacobsson, S., Clémençon, H. & Miller, O.K. Jr. (2002) One hundred and seventeen clades of euagarics. Molecular Phylogenetics and Evolution 23 (3): 357–400. https://doi.org/10.1016/S1055-7903(02)00027-1
  40. Mu, L.S. & Bau, T. (2023) A new species of Calocybe (Agaricales, Basidiomycota) from China. Phytotaxa 600 (2): 73–78. https://doi.org/10.11646/phytotaxa.600.2.2
  41. Nath, M., Barh, A., Sharma, A., Verma, P., Bairwa, R.K., Kamal, S. & Singh, A.R. (2023) Identification of eight high yielding strains via morpho-molecular characterization of thirty-three wild strains of Calocybe indica. Foods 12 (11): 2119. https://doi.org/10.3390/foods12112119
  42. Pegler, D.N., Lodge, D.J. & Nakasone, K.K. (1998) The pantropical genus Macrocybe gen. nov. Mycologia 90 (3): 494–504. https://doi.org/10.1080/00275514.1998.12026934
  43. Purkayastha, R.P. & Chandra, A. (1974) New species of edible mushroom from India. Transactions of the British Mycological Society 62 (2): 415–418. https://doi.org/10.1016/s0007-1536(74)80052-5
  44. Qi, Y., Xu, A., Zhou, Y., Bi, K., Qin, W., Guo, H. & Yu, X. (2022) Morphological and phylogenetic studies of three new species of Calocybe (Agaricales, Basidiomycota) from China. Diversity 14 (8): 643. https://doi.org/10.3390/d14080643
  45. Rao, G., Hu, J.J., Zhang, Z.H., Qi, Z.X., Zhang, B. & Li, Y. (2021) Gerhardtia foliicola (Agaricales, Lyophyllaceae), a specific dweller on leaf litter from northern China. Nova Hedwigia 113 (3–4): 491–505. https://doi.org/10.1127/nova_hedwigia/2021/0660
  46. Razzaq, F., Haqnawaz, M., Shahid, R., Ashraf, U., Afshan, N.U.S., Khalid, A.N. & Niazi, A.R. (2026) Phylogenetic reidentification of Calocybe indica to Macrocybe indica and new records of Calocybe erminea in Pakistan. Microscopy Research and Technique 89 (1): 100–108. https://doi.org/10.1002/jemt.70055
  47. Ronquist, F. & Huelsenbeck, J.P. (2003) MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19 (12): 1572–1574. https://doi.org/10.1093/bioinformatics/btg180
  48. Shashikant, M., Bains, A., Chawla, P., Fogarasi, M. & Fogarasi, S. (2022) The current status, bioactivity, food, and pharmaceutical approaches of Calocybe indica: A review. Antioxidants 11 (6): 1145. https://doi.org/10.3390/antiox11061145
  49. Singer, R. (1948) New and interesting species of Basidiomycetes. II. Papers of the Michigan Academy of Sciences 32: 103–150.
  50. Song, H.B. & Bau, T. (2023) Conocybe section Pilosellae in China: reconciliation of taxonomy and phylogeny reveals seven new species and a new record. Journal of Fungi 9 (9): 924. https://doi.org/10.3390/jof9090924
  51. Vizzini, A., Angelini, C. & Ercole, E. (2017) Is the species diversity in the lyophylloid genera Calocybella and Gerhardtia (Agaricales, Basidiomycota) underestimated? Two new species from the Dominican Republic. Phytotaxa 291 (4): 241–252. https://doi.org/10.11646/phytotaxa.291.4.1
  52. Vizzini, A., Consiglio, G., Setti, L. & Ercole, E. (2015) Rugosomyces pudicus and Calocybella, a new genus for Lyophyllaceae (Agaricales). IMA Fungus 6 (1): 1–11. https://doi.org/10.5598/imafungus.2015.06.01.01
  53. Wang, X.H., Cai, Q., Yu, F.M., Yang, Z.L., Zhou, S.Y., Wang, Z.R., Cui, Y.-Y., Wang, Y., Zhang, P., Cao, S.Q., Zhu, X.T., Lei, L., Lu, J.R., Li, J.N., Wang, G.S., Jia, L.K., Mu, L.H., Li, G.M., Li, M.X., Yang, B.X., Feng, W.C., Liu, Z.W., Li, C.J.Y., Xu, R.J., Bao, S.X., Li, T.S., Jia, P.S., Wu, D.M., Gao, N., Hyde, K.D., Wu, G. & Zhao, Q. (2025) New and notable taxa of Basidiomycota on the Qinghai–Xizang Plateau and its surrounding areas. Fungal Diversity 133 (1): 235–437. https://doi.org/10.1007/s13225-025-00558-x
  54. Watling, R. & Ginns, J. (1998) E.J.H. Corner, 1906–1996. Mycologia 90 (4): 732–737. https://doi.org/10.1080/00275514.1998.12026963
  55. Wei, S.W., Lu, B.Y., Wang, Y., Dou, W.J., Wang, Q. & Li, Y. (2023) Morphology and phylogeny of lyophylloid mushrooms in China with description of four new species. Journal of Fungi 9 (1): 77. https://doi.org/10.3390/jof9010077
  56. White, T.J., Bruns, T., Lee, S. & Taylor, J. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis, M.A., Gelfand, D.H., Sninsky, J.J. & White, T.J. (Eds.) PCR protocols: A guide to methods and applications. Academic Press, San Diego, pp. 315–322. https://doi.org/10.1016/b978-0-12-372180-8.50042-1
  57. Xu, J., Yu, X., Zhang, C. & Li, Y. (2019) Two new species of Calocybe (Lyophyllaceae) from northeast China. Phytotaxa 425 (4): 219–232. https://doi.org/10.11646/phytotaxa.425.4.3

How to Cite

Seelan, J.S.S., Vizzini, A., Wasti, I.G., Kasmin, J., Usimin, N.A.B. & Shahbaz, M. (2026) Calocybe kinabaluensis (Lyophyllaceae, Agaricales), a new species from Kinabalu UNESCO Global Geopark, Sabah, Northern Borneo, Malaysia. Phytotaxa 759 (3): 227–244. https://doi.org/10.11646/phytotaxa.759.3.3