Abstract
The taxonomic status of Hayata Aver. has long been a subject of debate, with confusion and uncertainty regarding its relationship to Zeuxine Lindl. and Cheirostylis Blume. Here, we conducted phylogenetic analyses using complete plastome data and a combination of traditional molecular markers (nrITS, matK, psaB, rbcL and trnL-trnF). Both plastid and nuclear datasets consistently resolved Hayata as sister to Zeuxine, with strong support from plastid data and moderate support from nrITS. Our results support the recognition of Hayata as an independent genus, distinct from both Zeuxine and Cheirostylis.
References
- Averyanov, L.V. (2009) Hayata glandulifera (Orchidaceae), new genus and species from northern Vietnam. Taiwania 54 (4): 311–316.
- Chase, M.W., Cameron, K.M., Freudenstein, J.V., Pridgeon, A.M., Salazar, G., Van den Berg, C. & Schuiteman, A. (2015) An updated classification of Orchidaceae. Botanical journal of the Linnean Society 177 (2): 151–174. https://doi.org/10.1111/boj.12234
- Chen, S.P., Tian, H.Z., Guan, Q.X., Zhai, J.W., Zhang, G.Q., Chen, L.J., Liu, Z.J. & Li, M.H. (2019) Molecular systematics of Goodyerinae (Cranichideae, Orchidoideae, Orchidaceae) based on multiple nuclear and plastid regions. Molecular phylogenetics and evolution 139: 106542. https://doi.org/10.1016/j.ympev.2019.106542
- Fu, X.Y. & Xiong, C. (2024) Cheirostylis sherriffii, a newly recorded species of Orchidaceae from China (in Chinese). Subtropical Plant Science 2024, 53 (5): 471.
- Jin, J.J., Yu, W.B., Yang, J.B., Song, Y., de Pamphilis, C.W., Yi, T.S. & Li, D.Z. (2020) GetOrganelle: A fast and versatile toolkit for accurate de novo assembly of organelle genomes. Genome Biology 21: 241. https://doi.org/10.1186/s13059-020-02154-5
- Jin, W.T., Xiang, X.G. & Jin, X.H. (2015) Generic delimitation of Orchidaceae from China: current situation and perspective. Biodiversity Science 23: 237–242. https://doi.org/10.17520/biods.2014268
- Katoh, K., Misawa, K., Kuma, K. & Miyata, T. (2002) MAFFT: A novel method for rapid multiple sequence alignment based on fast Fourier transform. Nucleic Acids Research 30: 3059–3066. https://doi.org/10.1093/nar/gkf436
- Miller, M.A., Pfeiffer, W. & Schwartz, T. (2010) Creating the CIPRES Science Gateway for inference of large phylogenetic trees. In: 2010 Gateway Computing Environments Workshop (GCE). IEEE, New Orleans, pp. 1–8. https://doi.org/10.1109/GCE.2010.5676129
- Qu, X.J., Moore, M.J., Li, D.Z. & Yi, T.S. (2019) PGA: A software package for rapid, accurate, and flexible batch annotation of plastomes. Plant Methods 15: 50. https://doi.org/10.1186/s13007-019-0435-7
- Ronquist, F., Teslenko, M., Van Der Mark, P., Ayres, D.L., Darling, A., Höhna, S., Larget, B., Liu, L., Suchard, M.A. & Huelsenbeck, J.P. (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology 61: 539–542. https://doi.org/10.1093/sysbio/sys029
- Stamatakis, A., Hoover, P. & Rougemont, J. (2008) A rapid bootstrap algorithm for the RAxML web-servers. Systematic Biology 75: 758–771. https://doi.org/10.1080/10635150802429642
- Swofford, D. (2003) PAUP: phylogenetic analysis using parsimony and other methods, version 4. Sinauer Associates, Sunderland.
- Tu, X.D., Liu, D.K., Xu, S.W., Zhou, C.Y., Gao, X.Y., Zeng, M.Y., Zhang, S., Chen, J.L., Ma, L., Zhou, Z., Huang, M.Z., Chen, S.P., Liu, Z.J., Lan, S.R. & Li, M.H. (2021) Plastid phylogenomics improves resolution of phylogenetic relationship in the Cheirostylis and Goodyera clades of Goodyerinae (Orchidoideae, Orchidaceae). Molecular Phylogenetics and Evolution 164: 107269. https://doi.org/10.1016/j.ympev.2021.107269
- Zhang, D., Gao, F., Jakovlić, I., Zou, H., Zhang, J., Li, W.X. & Wang, G.T. (2020) PhyloSuite: An integrated and scalable desktop platform for streamlined molecular sequence data management and evolutionary phylogenetics studies. Molecular Ecology Resources 20 (1): 348–355. https://doi.org/10.1111/1755-0998.13096
- Zhou, X.X., Cheng, Z.Q., Liu, Q.X., Zhang, J.L., Hu, A.Q., Huang, M.Z., Hu, C. & Tian, H.Z. (2016) An updated checklist of Orchidaceae for China, with two new national records. Phytotaxa 276: 1–148. https://doi.org/10.11646/phytotaxa.276.1.1
- Zeng, M.Y., Chen, J.L., Huang, M.Z. & Li, M.H. (2024) Phylogenetic position of Vanda coelestis (Rchb. f.) Motes (Orchidaceae; Aeridinae): evidence from complete plastome and combined traditional sequences. Phytotaxa 638 (3): 285–293. https://doi.org/10.11646/phytotaxa.638.3.8
