Skip to main content Skip to main navigation menu Skip to site footer
Type: Article
Published: 2026-04-23
Page range: 185-196
Abstract views: 0
PDF downloaded: 0

Thelotrema maharashtrense (Graphidaceae), a new lichen-forming species from the Western Ghats of India

Biodiversity and Palaeobiology (Fungi) Gr., MACS Agharkar Research Institute, G. G. Agarkar Road, Pune, 411 004, Maharashtra, India; Faculty of Science, Savitribai Phule Pune University, Ganeshkhind, Pune, 411007, Maharashtra, India
Biodiversity and Palaeobiology (Fungi) Gr., MACS Agharkar Research Institute, G. G. Agarkar Road, Pune, 411 004, Maharashtra, India; Faculty of Science, Savitribai Phule Pune University, Ganeshkhind, Pune, 411007, Maharashtra, India
Botanischer Garten und Botanisches Museum, Freie Universität Berlin, 14195 Berlin, Germany
Lichenized Ascomycota Graphidales Crustose lichen Polyphasic taxonomy Phylogeny Lichens

Abstract

This study describes a new lichenized species, Thelotrema maharashtrense, from the Western Ghats of Maharashtra, India, integrating morphological, chemical, and phylogenetic methods. Thelotrema maharashtrense is characterised by a loosely corticate thallus, prominent apothecia with an only rarely apically free, basally I+ amyloid exciple, 1-spored asci producing large, muriform ascospores, and lack of lichen substances. Analyses based on a concatenated mtSSU, LSU and rpb2 data set placed T. maharashtrense in a well-supported monophyletic clade allied to T. adjectum. This is the first molecular phylogenetic study of Thelotrema from India.

References

  1. Acharius, E. (1803) Methodus qua omnes detectos lichenes secundum organa carpomorpha ad genera, species et varietates redigere atque observationibus illustrare tentavit. Erik Acharius, Stockholm, 394 pp. https://doi.org/10.5962/bhl.title.79411
  2. Ansil, P.A., Rajeshkumar, K.C., Lücking, R., Gaikwad, S. & Sharma, B. (2025) Resolving the phylogeny of Diorygma aeolum, along with its photobiont Trentepohlia species from the Western Ghats, India. Studies in Fungi 10 (1): 1–10. https://doi.org/10.48130/sif-0025-0022
  3. Ansil, P.A., Rajeshkumar, K.C., Lücking, R., Paraparath, S.O. & Sharma, B. (2024) Molecular studies of Allographa effusosoredica sp. nov. (Graphidaceae) along with its Trentepohlia photobiont and a comprehensive checklist for Indian Allographa. Phytotaxa 664 (1): 31–45. https://doi.org/10.11646/phytotaxa.664.1.3
  4. Ansil, P.A., Rajeshkumar, K.C., Sharma, B., Lücking, R. & Hawksworth, D.L. (2023a) Phylogenetic placement and reappraisal of Diorygma karnatakense including the new synonym Diorygma dandeliense, from Maharashtra, India. The Lichenologist 55 (2): 59–67. https://doi.org/10.1017/S0024282923000087
  5. Ansil, P.A., Rajeshkumar, K.C., Sruthi, O.P., Gaikwad, S. & Sharma, B. (2023b) Decoding the evolutionary association among lichen symbionts in Dyplolabia afzelii from the Western Ghats, India. Microbial Biosystems 8 (2): 17–24. https://doi.org/10.21608/mb.2023.241013.1084
  6. Dumortier, B.C.J. (1822) Commentationes botanicae. Tournay, 116 pp.
  7. Katoh, K., Rozewicki, J. & Yamada, K.D. (2019) MAFFT online service: multiple sequence alignment, interactive sequence choice and visualization. Briefings in Bioinformatics 20 (4): 1160–1166. https://doi.org/10.1093/bib/bbx108
  8. Kraichak, E., Lücking, R., Aptroot, A., Beck, A., Dornes, P., John, V., Lendemer, J.C., Nelsen, M.P., Neuwirth, G., Nutakki, A., Parnmen, S., Sohrabi, M., Tønsberg, T. & Lumbsch, H.T. (2015) Hidden diversity in the morphologically variable script lichen (Graphis scripta) complex (Ascomycota, Ostropales, Graphidaceae). Organisms Diversity and Evolution 15: 447–458. https://doi.org/10.1007/s13127-015-0219-5
  9. Kuraku, S., Zmasek, C.M., Nishimura, O. & Katoh, K. (2013) Leaves facilitates on-demand exploration of metazoan gene family trees on MAFFT sequence alignment server with enhanced interactivity. Nucleic Acids Research 41 (W1): W22–W28. https://doi.org/10.1093/nar/gkt389
  10. Larsson, A. (2014) AliView: a fast and lightweight alignment viewer and editor for large datasets. Bioinformatics 30 (22): 3276–3278. https://doi.org/10.1093/bioinformatics/btu531
  11. Lumbsch, H.T., Mangold, A., Martín, M.P. & Elix, J.A. (2008) Species recognition and phylogeny of Thelotrema species in Australia (Ostropales, Ascomycota). Australian Systematic Botany 21 (3): 217–227. https://doi.org/10.1071/SB07049
  12. Mangold, A., Elix, J. & Lumbsch, H.T. (2009) Thelotremataceae. In: Flora of Australia, Vol. 57, Lichens 5. CSIRO Publishing, Melbourne, pp. 195–310.
  13. Mangold, A., Martin, M.P., Lücking, R. & Lumbsch, H.T. (2008) Molecular phylogeny suggests synonymy of Thelotremataceae within Graphidaceae (Ascomycota: Ostropales). Taxon 57 (2): 476–486.
  14. Matsumoto, T. (2000) Taxonomic studies of the Thelotremataceae (Graphidales, lichenized Ascomycota) in Japan (1) genus Thelotrema. Journal of the Hattori Botanical Laboratory 88: 1–50. https://doi.org/10.18968/jhbl.88.0_1
  15. Morgulis, A., Coulouris, G., Raytselis, Y., Madden, T.L., Agarwala, R. & Schäffer, A.A. (2008) Database indexing for production MegaBLAST searches. Bioinformatics 24 (16): 1757–1764. https://doi.org/10.1093/bioinformatics/btn322
  16. Nylander, J.A.A. (2004) MrModeltest 2.3. Program distributed by the author. Evolutionary Biology Centre, Uppsala University. Available from: http://www.abc.se/~nylander/ (accessed 29 March 2024)
  17. Nylander, W. (1873) Lichenes insularum Andaman. Bulletin de la Société Linnéenne de Normandie 7: 162–182.
  18. Nylander, W. (1890) Lichenes Japoniae. Accedunt observationibus lichenes insulae Labuan. Paris, 122 pp.
  19. Orange, A., James, P.W. & White, F.J. (2001) Microchemical methods for the identification of lichens. British Lichen Society, London, 101 pp.
  20. Rivas Plata, E., Lücking, R. & Lumbsch, H.T. (2012) A new classification for the family Graphidaceae (Ascomycota: Lecanoromycetes: Ostropales). Fungal Diversity 52 (1): 107–121. https://doi.org/10.1007/s13225-011-0135-8
  21. Rivas Plata, E., Lücking, R., Sipman, H.J.M., Mangold, A., Kalb, K. & Lumbsch, H.T. (2010) A world-wide key to the thelotremoid Graphidaceae, excluding the Ocellularia—Myriotrema—Stegobolus clade. The Lichenologist 42 (2): 139–185. https://doi.org/10.1017/S0024282909990491
  22. Rambaut, A. (2014) FigTree. Available from: http://tree.bio.ed.ac.uk/software/figtree/ (accessed 28 October 2025)
  23. Ronquist, F., Teslenko, M., Van Der Mark, P., Ayres, D.L., Darling, A., Höhna, S., Larget, B., Liu, L., Suchard, M.A. & Huelsenbeck, J.P. (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology 61 (3): 539–542. https://doi.org/10.1093/sysbio/sys029
  24. Sinha, G.P., Nayaka, S. & Mishra, G.K. (2024) A comprehensive checklist of lichens from India—2024. Cryptogam Biodiversity and Assessment 8 (2): 1–34.
  25. Stamatakis, A. (2006) RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics 22 (21): 2688–2690. https://doi.org/10.1093/bioinformatics/btl446
  26. Stamatakis, A., Hoover, P. & Rougemont, J. (2008) A rapid bootstrap algorithm for the RAxML web servers. Systematic Biology 57: 758–771. https://doi.org/10.1080/10635150802429642
  27. Stizenberger, E. (1862) Beitrag zur Flechtensystematik. Bericht über die Thätigkeit der St. Gallischen Naturwissenschaftlichen Gesellschaft 1861–1862: 124–182.
  28. Trifinopoulos, J., Nguyen, L.T., von Haeseler, A. & Minh, B.Q. (2016) W-IQ-TREE: a fast online phylogenetic tool for maximum likelihood analysis. Nucleic Acids Research 44 (W1): W232–W235. https://doi.org/10.1093/nar/gkw256
  29. Vilgalys, R. & Hester, M. (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. Journal of Bacteriology 172 (8): 4238–4246. https://doi.org/10.1128/jb.172.8.4238-4246.1990
  30. Weerakoon, G., Wijeyaratne, S.C., Wolseley, P.A., Rivas Plata, E., Lücking, R. & Lumbsch, H.T. (2012) Six new species of Graphidaceae from Sri Lanka. The Bryologist 115: 74–83. https://doi.org/10.1639/0007-2745-115.1.74
  31. Zoller, S., Scheidegger, C. & Sperisen, C. (1999) PCR primers for the amplification of mitochondrial small subunit ribosomal DNA of lichen-forming ascomycetes. The Lichenologist 31 (5): 511–516. https://doi.org/10.1006/lich.1999.0220

How to Cite

Ansil, P.A., Rajeshkumar, K.C. & Lücking, R. (2026) Thelotrema maharashtrense (Graphidaceae), a new lichen-forming species from the Western Ghats of India. Phytotaxa 752 (3): 185–196. https://doi.org/10.11646/phytotaxa.752.3.1