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Issue: Vol. 750 No. 4: 9 Apr. 2026
Type: Article
Published: 2026-04-09
DOI: 10.11646/phytotaxa.750.4.3
Page range: 253-268
Abstract views: 0
PDF downloaded: 0

A new species of Mariannaea isolated from the fruiting bodies of Morchella sextelata in Hebei Province, China

College of Landscape Architecture, Changchun University, Jilin, PR China, 130022
College of Landscape Architecture, Changchun University, Jilin, PR China, 130022
College of Landscape Architecture, Changchun University, Jilin, PR China, 130022
College of Landscape Architecture, Changchun University, Jilin, PR China, 130022
College of Landscape Architecture, Changchun University, Jilin, PR China, 130022
College of Landscape Architecture, Changchun University, Jilin, PR China, 130022
College of Landscape Architecture, Changchun University, Jilin, PR China, 130022
Department of Plant and Soil Sciences, Faculty of Science, Engineering and Agriculture, University of Venda, Thohoyandou 0950, South Africa
College of Landscape Architecture, Changchun University, Jilin, PR China, 130022
Mariannaea mycoparasite pathogenicity identification novel species Identification New taxon Phylogenetic analyses Fungi

Abstract

Morels (Morchella spp.), as rare and economically valuable edible mushrooms, have been rapidly cultivated in China and other regions in recent years, becoming a significant part of the agricultural economy. However, with the expansion of cultivation areas, morel diseases have become increasingly prominent. In particular, the white mold disease outbreak has severely restricted yield and quality improvement. A novel species (Mariannaea parasitica) and a new record of Mariannaea (Mariannaea parasitica) are reported from white mold-diseased fruiting bodies of Morchella based on morphology and phylogenetic analysis of ITS and LSU. The new species is characterized by erect, septate, hyaline, irregularly branched conidiophores, which often bear verticillate branches and usually produce 3–6 phialides. The phialides are ampulliform-lageniform, while the conidia are hyaline, smooth-walled, ellipsoidal to ovoid, with one side concave or flattened. This study broadens our understanding of the diversity of pathogen species in cultivated morels.

References

  1. Bao, D.F., Hyde, K.D., Maharachchikumbura, S.S.N., Perera, R.H., Thiyagaraja, V., Hongsanan, S., Wanasinghe, D.N., Shen, H.W., Tian, X.G., Yang, L.Q., Nalumpang, S. & Luo, Z.L. (2023) Taxonomy, phylogeny and evolution of freshwater Hypocreomycetidae (Sordariomycetes). Fungal Diversity 121: 1–94. https://doi.org/10.1007/s13225-023-00521-8
  2. Cai, L., Kurniawati, E. & Hyde, K.D. (2009) Morphological and molecular characterization of Mariannaea aquaticola sp. nov. collected from freshwater habitats. Mycological Progress 9: 337–343. https://doi.org/10.1007/s11557-009-0641-1
  3. Crous, P.W., Carnegie, A.J., Wingfield, M.J., Sharma, R., Mughini, G., Noordeloos, M.E., Santini, A., Shouche, Y.S., Bezerra, J.D.P., Dima, B., Guarnaccia, V., Imrefi, I., Jurjević, Ž., Knapp, D.G., Kovács, G.M., Magistà, D., Perrone, G., Rämä, T., Rebriev, Y.A., Shivas, R.G., Singh, S.M., Souza-Motta, C.M., Thangavel, R., Adhapure, N.N., Alexandrova, A.V., Alfenas, A.C., Alfenas, R.F., Alvarado, P., Alves, A.L., Andrade, D.A., Andrade, J.P., Barbosa, R.N., Barili, A., Barnes, C.W., Baseia, I.G., Bellanger, J.M., Berlanas, C., Bessette, A.E., Bessette, A.R., Biketova, A.Y., Bomfim, F.S., Brandrud, T.E., Bransgrove, K., Brito, A.C.Q., Cano-Lira, J.F., Cantillo, T., Cavalcanti, A.D., Cheewangkoon, R., Chikowski, R.S., Conforto, C., Cordeiro, T.R.L., Craine, J.D., Cruz, R., Damm, U., de Oliveira, R.J.V., de Souza, J.T., de Souza, H.G., Dearnaley, J.D.W., Dimitrov, R.A., Dovana, F., Erhard, A., Esteve-Raventós, F., Félix, C.R., Ferisin, G., Fernandes, R.A., Ferreira, R.J., Ferro, L.O., Figueiredo, C.N., Frank, J.L., Freire, K., García, D., Gené, J., Gêsiorska, A., Gibertoni, T.B., Gondra, R.A.G., Gouliamova, D.E., Gramaje, D., Guard, F., Gusmão, L.F.P., Haitook, S., Hirooka, Y., Houbraken, J., Hubka, V., Inamdar, A., Iturriaga, T., Iturrieta-González, I., Jadan, M., Jiang, N., Justo, A., Kachalkin, A.V., Kapitonov, V.I., Karadelev, M., Karakehian, J., Kasuya, T., Kautmanová, I., Kruse, J., Kušan, I., Kuznetsova, T.A., Landell, M.F., Larsson, K.H., Lee, H.B., Lima, D.X., Lira, C.R.S., Machado, A.R., Madrid, H., Magalhães, O.M.C., Majerova, H., Malysheva, E.F., Mapperson, R.R., Marbach, P.A.S., Martín, M.P., Martín-Sanz, A., Matočec, N., McTaggart, A.R., Mello, J.F., Melo, R.F.R., Mešić, A., Michereff, S.J., Miller, A.N., Minoshima, A., Molinero-Ruiz, L., Morozova, O.V., Mosoh, D., Nabe, M., Naik, R., Nara, K., Nascimento, S.S., Neves, R.P., Olariaga, I., Oliveira, R.L., Oliveira, T.G.L., Ono, T., Ordoñez, M.E., Ottoni, A.M., Paiva, L.M., Pancorbo, F., Pant, B., Pawłowska, J., Peterson, S.W., Raudabaugh, D.B., Rodríguez-Andrade, E., Rubio, E., Rusevska, K., Santiago, A., Santos, A.C.S., Santos, C., Sazanova, N.A., Shah, S., Sharma, J., Silva, B.D.B., Siquier, J.L., Sonawane, M.S., Stchigel, A.M., Svetasheva, T., Tamakeaw, N., Telleria, M.T., Tiago, P.V., Tian, C.M., Tkalčec, Z., Tomashevskaya, M.A., Truong, H.H., Vecherskii, M.V., Visagie, C.M., Vizzini, A., Yilmaz, N., Zmitrovich, I.V. & Zvyagina, E.A. (2019) Fungal Planet description sheets: 868–950. Persoonia 42: 291–473. https://doi.org/10.3767/persoonia.2019.42.11
  4. Feng, Y., Liu, Y., Hongsanan, S., Tarafder, E., Deng, X., Yang, T., Sun, J., Wen, T.C., Zeng, X.Y. & Liu, F. (2025) Two new species of Meliola (Meliolales, Sordariomycetes) from Guizhou Province, China. MycoKeys 122: 223–236. https://doi.org/10.3897/mycokeys.122.161471
  5. Fu, Y., Wu, H., Wang, S., Yu, Q., Tian, D. & Xu, X. (2023a) First report of Trichoderma atroviride causing rot of Morchella sextelata in Anhui Province, China. Crop Protection 168: 106206. https://doi.org/10.1016/j.cropro.2023.106206
  6. Fu, Y., Xu, X., Wu, H., Li, L., Wang, J., Sun, Y., Gu, L. & Yu, Q. (2023b) First Report of Clonostachys rosea Causing Rot of Morchella sextelata in Anhui Province, China. Mycologia 107: 1623. https://doi.org/10.1094/PDIS-08-22-1794-PDN
  7. Fu, S., Sun, J.E., Tarafder, E., Wijayawardene, N.N., Hu, Y., Wang, Y. & Li, Y. (2024) Pezizomycotina species associated with rotten plant materials in Guizhou Province, China. MycoKeys 106: 265–285. https://doi.org/10.3897/mycokeys.106.125920
  8. Fries, E.M. (1849) Summa vegetabilium Scandinaviae. Sect. Posterior.
  9. Gräfenhan, T., Schroers, H.J., Nirenberg, H.I. & Seifert, K.A. (2011) An overview of the taxonomy, phylogeny, and typification of nectriaceous fungi in Cosmospora, Acremonium, Fusarium, Stilbella, and Volutella. Stud Mycol 68: 79–113. https://doi.org/10.3114/sim.2011.68.04
  10. Hao, Y., Gen.Nuo, W. & Huang, Z. (2021) Mariannaea submersa sp. nov., with a new habitat and geographic record of Mariannaea catenulata. Mycosystema 40: 1286–1298. https://doi.org/10.13346/j.mycosystema.200186
  11. He, X.L., Peng, W.H., Miao, R.Y., Tang, J., Chen, Y., Liu, L.X., Wang, D. & Gan, B.C. (2017) White mold on cultivated morels caused by Paecilomyces penicillatus. FEMS microbiology letters 364. https://doi.org/10.1093/femsle/fnx037
  12. Hu, D.M., Wang, M. & Cai, L. (2017) Phylogenetic assessment and taxonomic revision of Mariannaea. Mycological Progress 16: 271–283. https://doi.org/10.1007/s11557-016-1252-2
  13. Hyde, K.D., Dong, Y., Phookamsak, R., Jeewon, R., Bhat, D.J., Jones, E.B.G., Liu, N.G., Abeywickrama, P.D., Mapook, A., Wei, D., Perera, R.H., Manawasinghe, I.S., Pem, D., Bundhun, D., Karunarathna, A., Ekanayaka, A.H., Bao, D.F., Li, J., Samarakoon, M.C., Chaiwan, N., Lin, C.G., Phutthacharoen, K., Zhang, S.N., Senanayake, I.C., Goonasekara, I.D., Thambugala, K.M., Phukhamsakda, C., Tennakoon, D.S., Jiang, H.B., Yang, J., Zeng, M., Huanraluek, N., Liu, J.K., Wijesinghe, S.N., Tian, Q., Tibpromma, S., Brahmanage, R.S., Boonmee, S., Huang, S.K., Thiyagaraja, V., Lu, Y.Z., Jayawardena, R.S., Dong, W., Yang, E.F., Singh, S.K., Singh, S.M., Rana, S., Lad, S.S., Anand, G., Devadatha, B., Niranjan, M., Sarma, V.V., Liimatainen, K., Aguirre-Hudson, B., Niskanen, T., Overall, A., Alvarenga, R.L.M., Gibertoni, T.B., Pfliegler, W.P., Horváth, E., Imre, A., Alves, A.L., da Silva Santos, A.C., Tiago, P.V., Bulgakov, T.S., Wanasinghe, D.N., Bahkali, A.H., Doilom, M., Elgorban, A.M., Maharachchikumbura, S.S.N., Rajeshkumar, K.C., Haelewaters, D., Mortimer, P.E., Zhao, Q., Lumyong, S., Xu, J. & Sheng, J. (2020) Taxonomic and phylogenetic contributions on genera and species of fungal taxa. Fungal Diversity 100: 5–277. https://doi.org/10.1007/s13225-020-00439-5
  14. Khan, M.B., Zeng, X.Y., Tarafder, E., Ali, S., Wei, D.P. & Wen, T.C. (2025) Two new species of Meliola from Yunnan Province China. MycoKeys 121: 79–92. https://doi.org/10.3897/mycokeys.121.158055
  15. Lan, Y.F., Cong, Q.Q., Wang, Q.W., Tang, L.N., Li, X.M., Yu, Q.W., Cui, X., An, X.R., Yu, C.X., Kong, F.H. & Li, X.D. (2020) First Report of Cladobotryum protrusum Causing Cobweb Disease on Cultivated Morchella importuna. Plant Dis. 104: 977. https://doi.org/10.1094/PDIS-08-19-1611-PDN
  16. Lakshmi, M.A., Bishnoi, S., Ho, C.L., Tarafder, E., Babu, B.K., Subbanna, A., Sahu, S. & Palnam, D.W. (2025) First pathogenomic insights into Ganoderma ellipsoideum as an emerging causal agent of basal stem rot in oil palm. Scientific Reports 15 (1). https://doi.org/10.1038/s41598-025-28488-3
  17. Li, Y., Chen, H. & Zhang, X. (2023) Cultivation, nutritional value, bioactive compounds of morels, and their health benefits: A systematic review. Frontiers in nutrition 10: 1159029. https://doi.org/10.3389/fnut.2023.1159029
  18. Liu, Q., Ma, H., Zhang, Y. & Dong, C. (2018) Artificial cultivation of true morels: current state, issues and perspectives. Critical reviews in biotechnology 38: 259–271. https://doi.org/10.1080/07388551.2017.1333082
  19. Liu, Z., Cong, Y., Sossah, F.L., Lu, Y., Kang, J. & Li, Y. (2023) Characterization and Genome Analysis of Cladobotryum mycophilum, the Causal Agent of Cobweb Disease of Morchella sextelata in China. Journal of Fungi 9: 411. https://doi.org/10.3390/jof9040411
  20. Lombard, L., Van Der Merwe, A., Groenewald, J.Z. & Crous, P.W. (2014) Lineages in Nectriaceae: re-evaluating the generic status of Ilyonectria and allied genera. Phytopathologia Mediterranea 53: 515–532.
  21. Lombard, L., van der Merwe, N.A., Groenewald, J.Z. & Crous, P.W. (2015) Generic concepts in Nectriaceae. Stud Mycol 80: 189–245. https://doi.org/10.1016/j.simyco.2014.12.002
  22. Luo, Z.L., Hyde, K.D., Liu, J.K., Maharachchikumbura, S.S.N., Jeewon, R., Bao, D.F., Bhat, D.J., Lin, C.G., Li, W.L., Yang, J., Liu, N.G., Lu, Y.Z., Jayawardena, R.S., Li, J.F. & Su, H.Y. (2019) Freshwater Sordariomycetes. Fungal Diversity 99: 451–660. https://doi.org/10.1007/s13225-019-00438-1
  23. Lv, B., Yu, S., Chen, Y., Yu, H. & Mo, Q. (2022) First Report of Lecanicillium aphanocladii Causing Rot of Morchella sextelata in China. Plant Disease 106. https://doi.org/10.1094/PDIS-12-21-2656-PDN
  24. Miao, Y., Qi, Y. & Peixin, H. (2020) Isolation and Identification of Pathogen of Morel White Rot. 142–145. https://doi.org/10.3389/fmicb.2023.1231353
  25. Nonaka, K., Kaneta, T., Ōmura, S. & Masuma, R. (2015) Mariannaea macrochlamydospora, a new hyphomycete (Nectriaceae) from soil in the Bonin Islands, Japan. Mycoscience 56: 29–33. https://doi.org/10.1016/j.myc.2014.02.001
  26. Noh, H. & Kim, S.H. (2025) Gliocladiopsis koreensis sp. nov., Ilyonectria koreensis sp. nov. & Mariannaea koreensis sp. nov. (Nectriaceae), Novel Fungi Isolated from Soil in Jeju Island and Upo Wetland in the Republic of Korea. Mycobiology 53 (2): 183–199. https://doi.org/10.1080/12298093.2025.2450905
  27. Okuda, T. & Yamamoto, K. (2000) Materials for the fungus flora of Japan (56) Mariannaea camptospora and M. elegans var. punicea from Japan. Mycoscience 41: 411–414. https://doi.org/10.1007/BF02463957
  28. Rehner, S.A. & Samuels, G.J. (1994) Taxonomy and phylogeny of Gliocladium analysed from nuclear large subunit ribosomal DNA sequences. Mycological Research 98: 625–634. https://doi.org/10.1016/S0953-7562(09)80409-7
  29. Rabenhorst, G.L. (1862) Cosmospora coccinea Rabenh. Hedwigia 2: 59–59.
  30. Samson, R.A. (1974) Paecilomyces and some allied Hyphomycetes. Studies in Mycology 6: 1–119.
  31. Rathnayaka, A.R., Tennakoon, D.S., Jones, G.E.B., Wanasinghe, D.N., Bhat, D.J., Priyashantha, A.K.H., Stephenson, S.L., Tibpromma, S. & Karunarathna, S.C. (2025) Significance of precise documentation of hosts and geospatial data of fungal collections, with an emphasis on plant-associated fungi. New Zealand Journal of Botany 63: 462–489. https://doi.org/10.1080/0028825X.2024.2381734
  32. Samson, R.A. & Bigg, W.L. (1988) A New Species of Mariannaea from California. Mycologia 80: 131–134. https://doi.org/10.1080/00275514.1988.12025512
  33. Samuels, G.J. & Seifert, K.A. (1991) Two new species of Nectria with Stilbella and Mariannaea anamorphs. Sydowia 43: 249–263.
  34. Shen, H.-W., Bao, D.-F., Boonmee, S., Su, X.-J., Tian, X.-G., Hyde, K.D. & Luo, Z.-L. (2023) Lignicolous Freshwater Fungi from Plateau Lakes in China (I): Morphological and Phylogenetic Analyses Reveal Eight Species of Lentitheciaceae, Including New Genus, New Species and New Records. Journal of Fungi.
  35. Shi, X., Liu, D., He, X., Liu, W. & Yu, F. (2022) Epidemic Identification of Fungal Diseases in Morchella Cultivation across China. Journal of fungi (Basel, Switzerland) 8. https://doi.org/10.3390/jof8101107
  36. Sun, J., Yu, S., Lu, Y., Liu, H. & Liu, X. (2023) Proposal of a new family Pseudodiploösporeaceae fam. nov. (Hypocreales) based on phylogeny of Diploöspora longispora and Paecilomyces penicillatus. Mycology 14: 60–73. https://doi.org/10.1080/21501203.2022.2143919
  37. Sun, J., Tarafder, E., Chen, T., Hongsanan, S., Chen, S., Norphanphoun, C., Karunarathna, S.C. & Zeng, X. (2026) Two novel species of sooty mould fungi (Capnodiales, Dothideomycetes) from China. MycoKeys 129: 23–41. https://doi.org/10.3897/mycokeys.129.182290
  38. Tarafder, E., Wenjun, Z., Karunarathna, S.C., Elgorban, A.M., Huilian, M., Nan, W., Zeng, X., Yong, W. & Tian, F.-H. (2024) Unveiling two new species of Trichoderma (Hypocreales, Hypocreaceae) that cause green mold disease on Stropharia rugosoannulata from Guizhou Province, China. MycoKeys 110: 361–383. https://doi.org/10.3897/mycokeys.110.134154
  39. Vilgalys, R. & Hester, M. (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. Journal of bacteriology 172: 4238–4246. https://doi.org/10.1128/jb.172.8.4238-4246.1990
  40. Vu, D., Groenewald, M., de Vries, M., Gehrmann, T., Stielow, B., Eberhardt, U., Al-Hatmi, A., Groenewald, J.Z., Cardinali, G., Houbraken, J., Boekhout, T., Crous, P.W., Robert, V. & Verkley, G.J.M. (2019) Large-scale generation and analysis of filamentous fungal DNA barcodes boosts coverage for kingdom fungi and reveals thresholds for fungal species and higher taxon delimitation. Studies in Mycology 92: 135–154. https://doi.org/10.1016/j.simyco.2018.05.001
  41. Wijesekara, W.M.S.N., Priyadarshani, T.D.C., Egodawatta, W.C.P., Menike, G.D.N., Weerasinghe, P.A., Tarafder, E., Elgorban, A.M. & Tibpromma, S. (2026) Biocontrol Efficacy of Trichoderma virens Against the White Root Rot Causing Pathogen Paramarasmius palmivorus in Field‐Grown Cowpea. New Zealand Journal of Botany 64 (1). https://doi.org/10.1002/nzb2.70038
  42. White, T.J., Bruns, T., Lee, S. & Taylor, J. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis, M.A., Gelfand, D.H., Sninsky, J.J. & White, T.J. (Eds.) PCR Protocols: a Guide to Methods and Applications. Academic Press, London, pp. 315–322.
  43. Wu, H., Chen, J., Li, J., Liu, Y., Park, H.J. & Yang, L. (2021) Recent Advances on Bioactive Ingredients of Morchella esculenta. Applied biochemistry and biotechnology 193: 4197–4213. https://doi.org/10.1007/s12010-021-03670-1
  44. Xie, J., Lu, S., Tarafder, E., Pan, Y., Peng, K., Zeng, X. & Tian, F. (2024b) Taxonomy, biological characterization and fungicide sensitivity assays of Hypomyces cornea sp. nov. causing cobweb disease on Auricularia cornea. Fungal Biology 128 (1): 1616–1625. https://doi.org/10.1016/j.funbio.2023.12.007
  45. Xie, J., Liu, X., Qin, Z., Mei, S., Tarafdar, E., Li, C., Zeng, X. & Tian, F. (2024a) Evolution and Related Pathogenic Genes of Pseudodiploöspora longispora on Morchella Based on Genomic Characterization and Comparative Genomic Analysis. https://doi.org/10.2139/ssrn.4673111
  46. Xu, C., Qian, L., Meng, Q. & Sun, Y. (2025) State-of-the-art review of morel: From chemistry to nutrition and health benefits. Journal of Food Composition and Analysis 141: 107351. https://doi.org/10.1016/j.jfca.2025.107351
  47. Xu, J., Yu, X., Suwannarach, N., Jiang, Y., Zhao, W. & Li, Y. (2021) Additions to Lyophyllaceae s.l. from China. Journal of Fungi 7. https://doi.org/10.3390/jof7121101
  48. Xu, J., Jiang, Y., Wang, T., Zhang, D., Li, X. & Hosen, M.I. (2023) Morphological characteristics and phylogenetic analyses revealed four new species of Agaricales from China. Frontiers in Microbiology 14. https://doi.org/10.3389/fmicb.2023.1118525
  49. Xu, Y., Tang, J., Wang, Y., He, X., Tan, H., Yu, Y., Chen, Y. & Peng, W. (2022) Large-scale commercial cultivation of morels: current state and perspectives. Applied microbiology and biotechnology 106: 4401–4412. https://doi.org/10.1007/s00253-022-12012-y
  50. Rabenhorst, G.L. (1862) Cosmospora Rabenh. Hedwigia.
  51. Zeng, Z.Q. & Zhuang, W.Y. (2014) A new holomorphic species of Mariannaea and epitypification of M. samuelsii. Mycological Progress 13: 980. https://doi.org/10.1007/s11557-014-0980-4
  52. Zhang, C.L., Jin, Z.W., Sun, C., Moodley, O., Xu, J.Z. & Li, Y. (2021) Morphological and phylogenetical analyses of pathogenic Hypomyces perniciosus isolates from Agaricus bisporus causing wet bubble disease in China. Phytotaxa 491: 115–130. https://doi.org/10.11646/phytotaxa.491.2.2
  53. Zhu, X., Ma, K., Sun, M., Zhang, J., Liu, L. & Niu, S. (2023) Isolation and identification of pathogens of Morchella sextelata bacterial disease. Frontiers in Microbiology 14: 1231353. https://doi.org/10.3389/fmicb.2023.1231353

How to Cite

Liang, R.-J., Liu, K.-L., Wang, Y.-W., Zhang, Y.-H., Han, S., Li, X.-F., Fu, Y., Naicker, O. & Zhang, C.-L. (2026) A new species of Mariannaea isolated from the fruiting bodies of Morchella sextelata in Hebei Province, China. Phytotaxa 750 (4): 253–268. https://doi.org/10.11646/phytotaxa.750.4.3