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Issue: Vol. 750 No. 3: 7 Apr. 2026
Type: Article
Published: 2026-04-07
DOI: 10.11646/phytotaxa.750.3.4
Page range: 186-196
Abstract views: 0
PDF downloaded: 0

A new freshwater hyphomycete species of Actinocladium in Stictographaceae (Asterinales) from Yunnan Province, China

College of Landscape and Horticulture, Yunnan Agricultural University, Kunming 650201, China; State Key Laboratory for Plant Diversity and Biogeography of East Asia, Yunnan Key Laboratory of Fungal Diversi-ty and Green Development, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China
State Key Laboratory for Plant Diversity and Biogeography of East Asia, Yunnan Key Laboratory of Fungal Diversi-ty and Green Development, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China
State Key Laboratory for Plant Diversity and Biogeography of East Asia, Yunnan Key Laboratory of Fungal Diversi-ty and Green Development, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China
College of Landscape and Horticulture, Yunnan Agricultural University, Kunming 650201, China
1 new species Dothideomycetes phylogeny taxonomy Fungi

Abstract

During an investigation into the diversity of lignicolous freshwater fungi along a north–south latitudinal gradient in the Asian region, two collections were obtained from submerged wood in freshwater habitats in Yunnan Province, China. Morphological comparison and multi-gene phylogenetic analysis of combined LSU, SSU and ITS sequence data identified the new collection as a novel species, namely Actinocladium jizhua. This species is characterized by macronematous, mononematous, cylindrical conidiophores, monoblastic conidiogenous cells, and distinctive stauriform conidia consisting of an obpyramidal basal part and usually 2–3 radiating arms. Phylogenetic analysis showed that A. jizhua formed a distinct branch within Stictographaceae and was closely related to A. aquaticum, but differs from A. aquaticum in having longer conidiophores and conidia. A comprehensive description and illustration of this new species are provided.

References

  1. Almeida, D.A.C., Barbosa, F.R. & Gusmão, L.F.P. (2012) Alguns fungos conidiais aquáticos-facultativos do bioma Caatinga. Acta botanica brasílica 26 (4): 924–932. https://doi.org/10.1590/S0102-33062012000400021
  2. Chethana, K.W.T., Manawasinghe, I.S., Hurdeal, V.G., Bhunjun, C.S., Appadoo, M.A., Gentekaki, E., Raspé, O., Promputtha, I. & Hyde, K.D. (2021) What are fungal species and how to delineate them? Fungal Diversity 109 (1): 1–25. https://doi.org/10.1007/s13225-021-00483-9
  3. Corda, A.C.J. (1837) Icones fungorum hucusque cognitorum (Vol. 1).
  4. Ehrenberg, C.G. (1819) Fungorum nova genera tria. Jahrbücher der Gewächskunde 1: 51–58.
  5. Ellis, M.B. (1971) Dematiaceous hyphomycetes. Commonwealth Mycological Institute, Kew England. https://doi.org/10.1079/9780851986180.0000
  6. Ellis, M.B. (1976) More Dematiaceous Hyphomycetes. CAB International Mycological Institute, Kew, England. https://doi.org/10.1079/9780851983653.0000
  7. Hall, T.A. (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 41: 95–98.
  8. Hernández-Restrepo, M., Gené, J., Castañeda-Ruiz, R.F., Mena-Portales, J., Crous, P.W. & Guarro, J. (2017) Phylogeny of saprobic microfungi from Southern Europe. Studies Mycology 86: 53–97. https://doi.org/10.1016/j.simyco.2017.05.002
  9. Hughes, S.J. (1951) Studies on micro-fungi. XII. Triposporium, Tripospermum, Ceratosporella and Tetraposporium (gen. nov.). Mycological Progress 46: 1–35.
  10. Hyde, K.D., McKenzie, E.H.C. & Koko, T.W. (2011) Towards incorporating anamorphic fungi in a natural classification—checklist and notes for 2010. Mycosphere 2: 1–88.
  11. Hyde, K.D., Fryar, S., Tian, Q., Bahkali, A.H. & Xu, J.C. (2016) Lignicolous freshwater fungi along a north—south latitudinal gradient in the Asian/Australian region; can we predict the impact of global warming on biodiversity and function? Fungal Ecology 19: 190–200. https://doi.org/10.1016/j.funeco.2015.07.002
  12. Hyde, K.D., Noorabadi, M.T., Thiyagaraja, V., He, M.Q., Johnston, P.R., Wijesinghe, S.N., Armand, A., Biketova, A.Y., Chethana, K.W.T., Erdoğdu, M., Ge, Z.W., Groenewald, J.Z., Hongsanan, S., Kušan, I., Leontyev, D.V., Li, D.W., Lin, C.G., Liu, N.G., Maharachchikumbura, S.S.N., Matočec, N., May, T.W., McKenzie, E.H.C., Mešić, A., Perera, R.H., Phukhamsakda, C., Piątek, M., Samarakoon, M.C., Selcuk, F., Senanayake, I.C., Tanney, J.B., Tian, Q., Vizzini, A., Wanasinghe, D.N., Wannasawang, N., Wijayawardene, N., Zhao, R.L., Abdel-Wahab, M.A., Abdollahzadeh, J., Abeywickrama, P.D., Abhinav, Absalan, S., Acharya, K., Afshari, N., Afshan, NS, Afzalinia, S., Ahmadpour, S.A., Akulov, O., Alizadeh, A., Alizadeh, M., Al-Sadi, A.M., Alves, A., Alves, V.C.S., Alves-Silva, G., Antonín, V., Aouali, S., Aptroot, A., Apurillo, C.C.S., Arias, R.M., Asgari, B., Asghari, R., Assis, D.M.A., Assyov, B., Atienza, V., Aumentado, H.D.R., Avasthi, S., Azevedo, E., Bakhshi, M., Bao, D.F., Baral, H.O., Barata, M., Barbosa, K.D., Barbosa, R.N., Barbosa, F.R., Baroncelli, R., Barreto, G.G., Baschien, C., Bennett, R.M., Bera, I., Bezerra, J.D.P., Bhunjun, C.S., Bianchinotti, M.V., Błaszkowski, J., Boekhout, T., Bonito, G.M., Boonmee, S., Boonyuen, N., Bortnikov, F.M., Bregant, C., Bundhun, D., Burgaud, G., Buyck, B., Caeiro, M.F., Cabarroi-Hernández, M., Cai, M.F., Cai, L., Calabon, M.S., Calaça, F.J.S., Callalli, M., Câmara, M.P.S., Cano-Lira, J., Cao, B., Carlavilla, J.R., Carvalho, A., Carvalho, T.G., Castañeda-Ruiz, R.F., Catania, M.D.V., Cazabonne, J., Cedeño-Sanchez, M., Chaharmiri-Dokhaharani, S., Chaiwan, N., Chakraborty, N., Cheewankoon, R., Chen, C., Chen, J., Chen, Q., Chen, Y.P., Chinaglia, S., Coelho-Nascimento, C.C., Coleine, C., CostaRezende, D.H., CortésPérez, A., Crouch, J.A., Crous, P.W., Cruz, R.H.S.F., Czachura, P., Damm, U., Darmostuk, V., Daroodi, Z., Das, K., Davoodian, N., Davydov, E.A., da Silva, G.A., da Silva, I.R., da Silva, R.M.F., da Silva, S.A.C., Dai, D.Q., Dai, Y.C., de Groot, M.D., De Kesel, A., De Lange, R., de Medeiros, E.V., de Souza, C.F.A., de Souza, F.A., dela Cruz, T.E.E., Decock, C., Delgado, G., Denchev, C.M., Denchev, T.T., Deng, Y.L., Dentinger, B.T.M., Devadatha, B., Dianese, J.C., Dima, B., Doilom, M., Dissanayake, A.J., Dissanayake, D.M.L.S., Dissanayake, L.S., Diniz, A.G., Dolatabadi, S., Dong, J.H., Dong, W., Dong, Z.Y., Drechsler-Santos, E.R., Druzhinina, I.S., Du, T.Y., Dubey, M.K., Dutta, A.K., Elliott, T.F., Elshahed, M.S., Egidi, E., Eisvand, P., Fan, L., Fan, X., Fan, X.L., Fedosova, A.G., Ferro, L.O., Fiuza, P.O., Flakus, A.W., Fonseca, E.O., Fryar, S.C., Gabaldón, T., Gajanayake, A.J., Gannibal, P.B., Gao, F., GarcíaSánchez, D., García-Sandoval, R., Garrido-Benavent, I., Garzoli, L., Gasca-Pineda, J., Gautam, A.K., Gené, J., GhobadNejhad, M., Ghosh, A., Giachini, A.J., Gibertoni, T.B., Gentekaki, E., Gmoshinskiy, V.I., GóesNeto, A., Gomdola, D., Gorjón, S.P., Goto, B.T., Granados-Montero, M.M., Griffith, G.W., Groenewald, M., Grossart, H.-P., Gu, Z.R., Gueidan, C., Gunarathne, A., Gunaseelan, S., Guo, S.L., Gusmão, L.F.P., Gutierrez, A.C., Guzmán-Dávalos, L., Haelewaters, D., Haituk, H., Halling, R.E., He, S.C., Heredia, G., HernándezRestrepo, M., Hosoya, T., Hoog, S.D., Horak, E., Hou, C.L., Houbraken, J., Htet, Z.H., Huang, S.K., Huang, W.J., Hurdeal, V.G., Hustad, V.P., Inácio, C.A., Janik, P., Jayalal, R.G.U., Jayasiri, S.C., Jayawardena, R.S., Jeewon, R., Jerônimo, G.H., Jin, J., Jones, E.B.G., Joshi, Y., Jurjević, Ž., Justo, A., Kakishima, M., Kaliyaperumal, M., Kang, G.P., Kang, J.C., Karimi, O., Karunarathna, S.C., Karpov, S.A., Kezo, K., Khalid, A.N., Khan, M.K., Khuna, S., Khyaju, S., Kirchmair, M., Klawonn, I., Kraisitudomsook, N., Kukwa, M., Kularathnage, N.D., Kumar, S., Lachance, M.A., Lado, C., Latha, K.P.D., Lee, H.B., Leonardi, M., Lestari, A.S., Li, C., Li, H., Li, J., Li, Q., Li, Y., Li, Y.C., Li, Y.X., Liao, C.F., Lima, J.L.R., Lima, J.M.S., Lima, N.B., Lin, L., Linaldeddu, B.T., Linn, M.M., Liu, F., Liu, J.K., Liu, J.W., Liu, S., Liu, S.L., Liu, X.F., Liu, X.Y., Longcore, J.E., Luangharn, T., Luangsaard, J.J., Lu, L., Lu, Y.Z., Lumbsch, H.T., Luo, L., Luo, M., Luo, Z.L., Ma, J., Madagammana, A.D., Madhushan, A., Madrid, H., Magurno, F., Magyar, D., Mahadevakumar, S., Malosso, E., Malysh, J.M., Mamarabadi, M., Manawasinghe, I.S., Manfrino, R.G., Manimohan, P., Mao, N., Mapook, A., Marchese, P., Marasinghe, D.S., Mardones, M., Marin-Felix, Y., Masigol, H., Mehrabi, M., MehrabiKoushki, M., Meiras-Ottoni, A., de Melo, R.F.R., Mendes-Alvarenga, R.L., Mendieta, S., Meng, Q.F., Menkis, A., Menolli, J.N., Mikšík, M., Miller, S.L., Moncada, B., Moncalvo, J.M., Monteiro, J.S., Monteiro, M., Mora-Montes, H.M., Moroz, E.L., Moura, J.C., Muhammad, U., Mukhopadhyay, S., Nagy, G.L., Najamul, S.A., Najafiniya, M., Nanayakkara, C.M., Naseer, A., Nascimento, E.C.R., Nascimento, S.S., Neuhauser, S., Neves, M.A., Niazi, A.R., Nie, Yong., Nilsson, R.H., Nogueira, P.T.S., Novozhilov, Y.K., Noordeloos, M., Norphanphoun, C., Nuñez, O.N., O’Donnell, R.P., Oehl, F., Oliveira, J.A., Oliveira, J.I., Oliveira, N.V.L., Oliveira, P.H.F., Orihara, T., Oset, M., Pang, K.L., Papp, V., Pathirana, L.S., Peintner, U., Pem, D., Pereira, O.L., PérezMoreno, J., Pérez-Ortega, S., Péter, G., Pires-Zottarelli, C.L.A., Phonemany, M., Phongeun, S., Pošta, A., Prazeres, J.F.S.A., Quan, Y., Quandt, C.A., Queiroz, M.B., Radek, R., Rahnama, K., Raj, K.N.A., Rajeshkumar, K.C., Rajwar, S., Ralaiveloarisoa, A.B., Rämä, T., Ramírez-Cruz, V., Rambold, G., Rathnayaka, A.R., Raza, M., Ren, G.C., Rinaldi, A.C., Rivas-Ferreiro, M., Robledo, G.L., Ronikier, A., Rossi, W., Rusevska, K., Ryberg, M., Safi, A., Salimi, F., Salvador-Montoya, C.A., Samant, B., Samaradiwakara, N.P., Sánchez-Castro, I., Sandoval-Denis, M., Santiago, A.L.C.M.A., Santos, A.C.D.S., Santos, L.A., Sarma, V.V., Sarwar, S., Savchenko, A., Savchenko, K., Saxena, R.K., Schoutteten, N., Selbmann, L., Ševčíková, H., Sharma, A., Shen, H.W., Shen, Y.M., Shu, Y.X., Silva, H.F., Silva-Filho, A.G.S., Silva, V.S.H., Simmons, D.R., Singh, R., Sir, E.B., Sohrabi, M., Souza, F.A., Souza-Motta, C.M., Sriindrasutdhi, V., Sruthi, O.P., Stadler, M., Stemler, J., Stephenson, S.L., Stoyneva-Gaertner, M.P., Strassert, J.F.H., Stryjak-Bogacka, M., Su, H.L., Sun, Y.R., Svantesson, S., Sysouphanthong, P., Takamatsu, S., Tan, T.H., Tanaka, K., Tang, C., Tang, X., Taylor, J.E., Taylor, P.W.J, Tennakoon, D.S., Thakshila, S.A.D., Thambugala, K.M., Thamodini, G.K., Thilanga, D., Thines, M., Tiago, P.V., Tian, X.G., Tian, W.H., Tibpromma, S., Tkalčec, Z., Tokarev, Y.S., Tomšovský, M., Torruella, G., Tsurykau, A., Udayanga, D., Ulukapı, M., Untereiner, W.A., Usman, M., Uzunov, B.A., Vadthanarat, S., Valenzuela, R., Van den Wyngaert, S., Van Vooren, N., Velez, P., Verma, R.K., Vieira, L.C., Vieira, W.A.S., Vinzelj, J.M., Tang, A.M.C. & Walker, A. (2024) The 2024 Outline of Fungi and fungus-like taxa. Mycosphere 15: 5146–6239. https://doi.org/10.5943/mycosphere/15/1/25
  13. Jayasiri, S.C., Hyde, K.D., Ariyawansa, H.A., Bhat, J., Buyck, B., Cai, L., Dai, Y.C., Abd-Elsalam, K.A., Ertz, D., Hidayat, I., Jeewon, R., Jones, E.B., Gareth, B.A.H., Karunarathna, S.C., Liu, J.K., Luangsa-ard, J.J., Lumbsch, H.T., Maharachchikumbura, S.S.N., McKenzie, E.H.C., Moncalvo, J.M., Masoomeh, G.N., Nilsson, H., Pang, K.L., Olinto, L., Pereira, A.J.L., Phillips, O.R., Raspé, A.W., Rollins, A.I., Romero, A.I., Etayo, J., Selçuk, F., Stephenson, S.L., Suetrong, S., Taylor, J.E., Tsui, C.K.M., Vizzini, A., Mohamed, A. & Promputtha, I. (2015) The Faces of Fungi database: fungal names linked with morphology, phylogeny and human impacts. Fungal Diversity 74 (1): 3–18. https://doi.org/10.1007/s13225-015-0351-8
  14. Jeewon, R. & Hyde, K.D. (2016) Establishing species boundaries and new taxa among fungi: recommendations to resolve taxonomic ambiguities. Mycosphere 7 (11): 1669–1677. https://doi.org/10.5943/mycosphere/7/11/4
  15. Katoh, K., Rozewicki, J. & Yamada, K.D. (2019) MAFFT online service: multiple sequence alignment, interactive sequence choice and visualization. Briefings in Bioinformatics 20: 1160–1166. https://doi.org/10.1093/bib/bbx108
  16. Kirschner, R., Chen, Z.C. & Oberwinkler, F. (2001) New records of ten species of hyphomycetes from Taiwan. Fungal Science 16 (3): 4.
  17. Kuthubutheen, A.J. & Nawawi, A. (1991) A new species of Ceratosporella and Triposporium lambdaseptatum (Matsush.) comb. nov. from Malaysia. Mycological Research 95: 158–162. https://doi.org/10.1016/S0953-7562(09)81005-8
  18. Luo, Z.L., Hyde, K.D., Liu, J.K., Bhat, D.J., Bao, D.F., Li, W.L. & Su, H.Y. (2018) Lignicolous freshwater fungi from China II: Novel Distoseptispora (Distoseptisporaceae) species from northwestern Yunnan Province and a suggested unified method for studying lignicolous freshwater fungi. Mycosphere 9 (3): 444–461. https://doi.org/10.5943/mycosphere/9/3/2
  19. Manoharachary, C., Kunwar, I.K., Suresh Kumar, G., Reddy, B.S. & Agarwal, D.K. (2010) Two new species of Actinocladium Ehrenb from India. Indian Phytopath 63 (1): 83–84.
  20. Matsushima, T. (1993) Matsushima Mycological Memoirs 7. Matsushima Fungus Collection, Kobe.
  21. Miller, M.A., Pfeiffer, W. & Schwartz, T. (2010) Creating the CIPRES Science Gateway for inference of large phylogenetic trees. In: Gateway Computing Environments Workshop 2010 (GCE). New Orleans, Louisiana, pp. 1–8. https://doi.org/10.1109/GCE.2010.5676129
  22. Miller, M.A., Schwartz, T., Pickett, B.E., He, S., Klem, E.B., Scheuermann, R.H., Passarotti, M, Kaufman, S. & O’Leary, M.A. (2015) A RESTful API for Access to Phylogenetic Tools via the CIPRES Science Gateway. Evolutionary Bioinformatics 11: EBO.S21501. https://doi.org/10.4137/EBO.S21501
  23. Mortimer, P.E., Lumyong, S. & Hyde, K.D. (2021) https://gmsmicrofungi.org: an online database providing updated information of microfungi in the Greater Mekong Subregion. Mycosphere 12 (1): 1513–1526. https://doi.org/10.5943/mycosphere/12/1/19
  24. Rambaut, A. (2012) FigTree v1.4. University of Edinburgh, Edinburgh.
  25. Ronquist, F., Paul, V.M., Daniel, L.A., Aaron, D., Sebastian, H., Bret, L., Liu, L. & Marc, A.S. & John, P.H. (2012) MRBAYES 3.2: Efficient Bayesian phylogenetic inference and model selection across a large model space. Systematic Biology 61 (3): 539–542. https://doi.org/10.1093/sysbio/sys029
  26. Senanayake, I.C., Rathnayaka, A.R., Marasinghe, D.S., Calabon, M.S., Gentekaki, E., Lee, H.B., Hurdeal, V.G., Pem, D., Dissanayake, L.S., Wijesinghe, S.N., Bundhun, D., Nguyen, T.T., Goonasekara, I.D., Abeywickrama, P.D., Bhunjun, C.S., Jayawardena, R.S., Wanasinghe, D.N., Jeewon, R., Bhat, D.J. & Xiang, M.M. (2020) Morphological approaches in studying fungi: Collection, examination, isolation, sporulation and preservation. Mycosphere 11 (1): 2678–2754. https://doi.org/10.5943/mycosphere/11/1/20
  27. Shenoy, B.D., Jeewon, R., Wu, W.P., Bhat, D.J. & Hyde, K.D. (2006) Ribosomal and RPB2 DNA sequence analyses suggest that Sporidesmium and morphologically similar genera are polyphyletic. Mycological Research 110: 916–928. https://doi.org/10.1016/j.mycres.2006.06.004
  28. Shenoy, B.D., Jeewon, R. & Hyde, K.D. (2007) Impact of DNA sequence-data on the taxonomy of anamorphic fungi. Fungal Diversity 26: 1–54.
  29. Stamatakis, A. (2014) RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 30 (9): 1312–1313. https://doi.org/10.1093/bioinformatics/btu033
  30. Tian, W.H., Liu, J.W., Jin, Y., Chen, Y.P., Zhou, Y.F., Wu, K., Su, P.W., Guo, X.Y., Wanasinghe, D.N., Hyde, K.D., Liu, J.K. & Maharachchikumbura, S.S.N. (2024) Morphological and phylogenetic studies of Ascomycota from gymnosperms in Sichuan Province, China. Mycosphere 15 (1): 1794–1900. https://doi.org/10.5943/mycosphere/15/1/16
  31. Vaidya, G., Lohman, D.J. & Meier, R. (2011) Sequence Matrix: concatenation software for the fast assembly of multi-gene datasets with character set and codon information. Cladistics 27 (2): 171–180. https://doi.org/10.1111/j.1096-0031.2010.00329.x
  32. Vilgalys, R. & Hester, M. (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. Journal of Bacteriology 172 (8): 4238–4246. https://doi.org/10.1128/jb.172.8.4238-4246.1990
  33. White, T.J., Bruns, T., Lee, S. & Taylor, J. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR Protocols: A Guide to Methods and Applications 18 (1): 315–322. https://doi.org/10.1016/B978-0-12-372180-8.50042-1
  34. Wu, W.P. & Zhuang, W.Y. (2005) Sporidesmium, Endophragmiella and related genera from China. Fungal Diversity Research Series 15: 1–351.
  35. Xu, R.J., Hyde, K.D., Li, J.N., Boonmee, S., Liu, N.G., Yang, J., Li, Y., Bao, D.F., Shen, H.W., Zhu, X.T., Zhu, Y.A., Li, T.S., Xu, K., Yu, F.M., Lu, J.R., Lei, L., Wu, N., Wu, D.M., Gao, N., Jia, P.S., He, X.L., Fatimah Al-Otibi, Zhou, D.Q., Liu, J.K., Lu, Y.Z., Luo, Z.L. & Yang, Z.L. (2025) Lignicolous freshwater fungi of the pan Qinghai-Xizang Plateau, China. Fungal Diversity 133: 23–234. https://doi.org/10.1007/s13225-025-00555-0
  36. Yang, J., Liu, L.L., Jones, E.G., Hyde, K.D., Liu, Z.Y., Bao, D.F., Liu, N.G., Li, W.L., Shen, H.W., Yu, X.D. & Liu, J.K. (2023) Freshwater fungi from karst landscapes in China and Thailand. Fungal Diversity 119 (1): 1–212. https://doi.org/10.1007/s13225-023-00514-7
  37. Yurchenko, E.O. (2001) On some wood-inhabiting dematiaceous hyphomycetes with remarkable conidia in Belarus. Mycena: An independent mycological journal 1 (2): 32–54.
  38. Zhao, G.C. & Li, N.L. (1997) Two new species of Microclava and Actinocladium (Hyphomycetes) from Yunnan. Mycosystema 16 (4): 270–273.

How to Cite

Geng, Y.-W., Xu, R.-J., Zhao, Q. & Zhu, Y.-A. (2026) A new freshwater hyphomycete species of Actinocladium in Stictographaceae (Asterinales) from Yunnan Province, China. Phytotaxa 750 (3): 186–196. https://doi.org/10.11646/phytotaxa.750.3.4