Abstract
Aureoboletus zunyiensis from Guizhou Province, China, is described hereby on the basis of morphology and molecular analysis. It is characterized by a brownish-red pileal surface, yellowish context, ovoid to reniform spores, and cystidia are covered with a pale yellow refractive substance. Multigene analysis based on nrLSU, ITS, and TEF1-α supported that the new species belongs to Aureoboletus. Photographs, microstructure diagram, and a phylogenetic tree showing relationships to closely related taxa are provided.
References
- Acharya, K., Tarafder, E., Dutta, A.K., Nandi, S., Pradhan, P., Sarkar, J., Paloi, S., Sikder, R. & Roy, A. (2017a) Contribution to the Macromycetes of West Bengal, India: 8–12. Research Journal of Pharmacy and Technology 10 (3): 823. https://doi.org/10.5958/0974-360X.2017.00155.X
- Acharya, K., Tarafder, E., Pradhan, P., Dutta, A.K., Paloi, S., Datta, M. & Roy, A. (2017b) Contribution to the Macromycetes of West Bengal, India: 18–22. Research Journal of Pharmacy and Technology 10 (9): 3061. https://doi.org/10.5958/0974-360x.2017.00543.1
- Alexandros, S. (2014) RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 30 (9): 1312–1313. https://doi.org/10.1093/bioinformatics/btu033
- Binder, M. (1999) Zur molekularen Systematik der Boletales: Boletineae und Sclerodermatineae subordo nov. PhD Thesis, University of Regensburg, Regensburg.
- Hosen, M.I., Feng, B., Wu, G., Zhu, X.T., Li, Y.C. & Yang, Z.L. (2013) Borofutus, a new genus of Boletaceae from tropical Asia: phylogeny, morphology and taxonomy. Fungal Diversity 58: 215–226. https://doi.org/10.1007/s13225-012-0211-8
- Katoh, K. & Standley, D.M. (2013) MAFFT multiple sequence alignment software version 7: Improvements in performance and usability. Molecular Biology and Evolution 30: 772–780. https://doi.org/10.1093/molbev/mst010
- Kornerup, A. & Wanscher, J.H. (1983) Methuen Handbook of Colour, 3rd ed. Eyre Methuen, London, UK, pp. 1–252.
- Das, K., Datta, S., Ghosh, A. & Vizzini, A. (2025) Multigene genealogy and morphology unveil one new genus, one new species and one new combination of boletoid mushrooms (Boletaceae) from Indian Himalaya. Taiwania 70 (1): 147–155. https://doi.org/10.6165/tai.2025.70.147
- Li, F., Zhao, K., Deng, Q.L., Zhang, M., Staehelin, C., Chen, X.X., Chen, H.Y. & Li, B.S. (2016) Three new species of Boletaceae from the Heishiding Nature Reserve in Guangdong Province, China. Mycological Progress 15: 1269–1283. https://doi.org/10.1007/s11557-016-1233-5
- Li, Y.C., Feng, B. & Yang, Z.L. (2011) Zangia, a new genus of Boletaceae supported by molecular and morphological evidence. Fungal Diversity 49: 125–143. https://doi.org/10.1007/s13225-011-0096-y
- Li, H., Tian, Y., Menolli, N., Ye, L., Karunarathna, S.C., Perez‐Moreno, J., Rahman, M.M., Rashid, M.H., Phengsintham, P., Rizal, L., Kasuya, T., Lim, Y.W., Dutta, A.K., Khalid, A.N., Huyen, L.T., Balolong, M.P., Baruah, G., Madawala, S., Thongklang, N., Hyde, K.D., Kirk, P.M., Xu, J., Sheng, J., Boa, E. & Mortimer, P.E. (2021) Reviewing the world’s edible mushroom species: a new evidence-based classification system. Comprehensive Reviews in Food Science and Food Safety 20: 1982–2014. https://doi.org/10.1111/1541-4337.12708
- Liu, S.L., Wang, X.W., Li, G.J., Deng, C.Y., Rossi, W., Leonardi, M., Liimatainen, K., Kekki, T., Niskanen, T., Smith, M.E., Ammirati, J., Bojantchev, D., Abdel-Wahab, M.A., Zhang, M., Tian, E.J., Lu, Y.Z., Zhang, J.Y., Ma, J., Dutta, A.K., Acharya, K., Du, T.Y., Xu, J.Z., Kim, J.S., Lim, Y.W., Gerlach, A., Zeng, N.K., Han, Y.X., Razaghi, P., Raza, M., Cai, L., Calabon, M.S., Jones, E.B.G., Saha, R., Kumar, T.K.A., Krishnapriya, K., Thomas, A., Kaliyaperumal, M., Kezo, K., Gunaseelan, S., Singh, S.K., Singh, P.N., Lagashetti, A.C., Pawar, K.S., Jiang, S.H., Zhang, C., Zhang, H., Qing, Y., Bau, T., Peng, X.C., Wen, T.C., Ramirez, N.A., Niveiro, N., Li, M.X., Yang, Z.L., Wu, G., Tarafder, E., Tennakoon, D.S., Kuo, C.H., da Silva, T.M., Souza-Motta, C.M., Bezerra, J.D.P., He, G., Ji, X.H., Suwannarach, N., Kumla, J., Lumyong, S., Wannathes, N., Rana, S., Hyde, K.D. & Zhou, L.W. (2024) Fungal diversity notes 1717–1817: taxonomic and phylogenetic contributions on genera and species of fungal taxa. Fungal Diversity 124: 1–216 https://doi.org/10.1007/s13225-023-00529-0
- Miller, M.A., Pfeiffer, W. & Schwartz, T. (2010) Creating the CIPRES Science Gateway for Inference of Large Phylogenetic Trees. In: 2010 Gateway Computing Environments Workshop (GCE). New Orleans, LA, USA, pp. 1–8. https://doi.org/10.1109/GCE.2010.5676129
- Murrill, W.A. (1909) The Boletaceae of North America—I. Mycologia 1 (1): 4–18. https://doi.org/10.1080/00275514.1909.12020569
- Nguyen, L.T., Schmidt, H.A., Haeseler, A. & Minh, B.Q. (2015) IQ-TREE: A fast and effective stochastic algorithm for estimating maximum-likelihood phylogenies. Molecular Biology and Evolution 32: 268–274. https://doi.org/10.1093/molbev/msu300
- Quélet, L. (1888) Flore Mycologique de la France. Tome 1. Librairie des Sciences Naturelles, Paris, 431 pp.
- Pouzar, Z. (1957) Nova genera macromycetum I. Česká Mykologie 11: 48–50.
- Rambaut, A., Suchard, M.A. & Xie, D. (2014) Drummond, A.J. Tracer v1.6. [http://beast.bio.ed.ac.uk/Tracer]
- Rehner, S.A. & Buckley, E. (2005) A Beauveria phylogeny inferred from nuclear ITS and EF1-α sequences: evidence for cryptic diversification and links to Cordyceps teleomorphs. Mycologia 97 (1): 84–98. https://doi.org/10.1080/15572536.2006.11832842
- Ronquist, F., Teslenko, M., Mark, P., Ayres, D.L., Darling, A., Höhna, S., Larget, B., Liu, L., Suchard, M.A. & Huelsenbeck, J.P. (2012) MrBayes 3.2: Efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology 61: 539–542. https://doi.org/10.1093/sysbio/sys029
- Steidinger, B., Crowther, T., Liang, J., Van Nuland, M., Werner, G., Reich, P., Nabuurs, G., De-Miguel, S., Zhou, M., Picard, N., Herault, B., Zhao, X., Zhang, C., Routh, D., Avitabile, V. & Peay, K. (2019) Climatic controls of decomposition drive the global biogeography of forest-tree symbioses. Nature 569: 404–408. https://doi.org/10.1038/s41586-019-1128-0
- Tarafder, E., Dutta, A.K., Pradhan, P., Mondal, B., Chakraborty, N., Paloi, S., Roy, A. & Acharya, K. (2017) Contribution to the Macromycetes of West Bengal, India: 13–17. Research Journal of Pharmacy and Technology 10 (4): 1123. https://doi.org/10.5958/0974-360X.2017.00203.7
- Tarafder, E., Dutta, A.K. & Acharya, K. (2022) New species and new record in Agaricus subg. Minores from India. Turkish Journal of Botany 46 (2): 183–195. https://doi.org/10.55730/1300-008x.2681
- Tarafder, E., Dutta, A.K., Karunarathna, S.C., He, M.Q., Tian, F. & Acharya, K. (2023) Agaricus sect. Xanthodermatei (Agaricales) of West Bengal, India. Phytotaxa 630 (4): 245–265. https://doi.org/10.11646/phytotaxa.630.4.1
- Vilgalys, R. & Hester, M. (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. Journal Of Bacteriology 172: 4238–4246. https://doi.org/10.1128/jb.172.8.4238-4246.1990
- White, T.J., Bruns, T., Lee, S. & Taylor, J. (1990) Amplification and direct sequencing of fungal ribosomal rNA genes for phylogenies. In: Innis, M.A., Gelfand, D.H., Sninsky, J.J. & White, T.J. (Eds.) PCR protocols: a guide to methods and applications. Academic, San Diego. https://doi.org/10.1016/b978-0-12-372180-8.50042-1
- Wu, G., Li, Y.C., Zhu, X.T., Zhao, K., Han, L.H., Cui, Y.Y., Li, F., Xu, J.P. & Yang, Z.L. (2016) One hundred noteworthy boletes from China. Fungal Diversity 81: 25–188. https://doi.org/10.1007/s13225-016-0375-8
- Wu, G., Li, H.J., Horak, E., Wu, K., Li, G.M. & Yang, Z.L. (2023) New taxa of Boletaceae from China. Mycosphere 14 (1): 745–776. https://doi.org/10.5943/mycosphere/14/1/9
- Xue, R., Zhang, X., Xu, C., Liang, Z.Q. & Zeng, N.K. (2023) The subfamily Xerocomoideae (Boletaceae, Boletales) in China. Studies in Mycology 106: 95–197. https://doi.org/10.3114/sim.2022.106.03
- Zeng, N.K., Zhang, M. & Liang, Z.Q. (2015) A new species and a new combination in the genus Aureoboletus (Boletales, Boletaceae) from southern China. Phytotaxa 222: 129–137. https://doi.org/10.11646/phytotaxa.222.2.5
- Zhou, F., Zhang, J., Liu, S., Liu, Q., Liu, C., Luo, X., Luo, X., Wang, L., Sun, D. & Hua, R. (2025) Aureoboletus pseudorussellii sp. nov., a newly discovered species in genus Aureoboletus of Zhejiang Province, China. Mycobiology 53 (3): 305–313. https://doi.org/10.1080/12298093.2025.2480406
- Zhang, M., Li, T.H., Wang, C.Q., Zeng, N.K. & Deng, W.Q. (2019) Phylogenetic overview of Aureoboletus (Boletaceae, Boletales), with descriptions of six new species from China. MycoKeys 61: 111. https://doi.org/10.3897/mycokeys.61.47520
- Zhang, D., Gao, F.L., Jakovlić, I., Zou, H., Zhang, J., Li, W.X. & Wang, GT. (2020) PhyloSuite: an integrated and scalable desktop platform for streamlined molecular sequence data management and evolutionary phylogenetics studies. Molecular Ecology Resources 20 (1): 348–355. https://doi.org/10.1111/1755-0998.13096
- Zhao, K. & Wu, G. (2023) Aureoboletus rubellus sp. nov. (Boletaceae) from Jiangxi Province, China. Phytotaxa 585 (2): 105–112. https://doi.org/10.11646/phytotaxa.420.1.5
- Zhu, X.T., Wu, G., Zhao, K., Halling, R.E. & Yang, Z.L. (2015) Hourangia, a new genus of Boletaceae to accommodate Xerocomus cheoi and its allied species. Mycological Progress 14: 37. https://doi.org/10.1007/s11557-015-1060-0
