Skip to main content Skip to main navigation menu Skip to site footer
Type: Article
Published: 2026-02-05
Page range: 138-152
Abstract views: 22
PDF downloaded: 1

Morpho-phylogenetic evidence reveals a new species and two known taxa of Neomyrmecridium from freshwater habitats in the Nujiang River, Yunnan, China

College of Agriculture and Biological Science, Dali University, Dali 671003, Yunnan, P.R. China
College of Agriculture and Biological Science, Dali University, Dali 671003, Yunnan, P.R. China
College of Agriculture and Biological Science, Dali University, Dali 671003, Yunnan, P.R. China
College of Agriculture and Biological Science, Dali University, Dali 671003, Yunnan, P.R. China; Co-Innovation Center for Cangshan Mountain and Erhai Lake Integrated Protection and Green Development of Yunnan Province, Dali University, Dali 671003, Yunnan, P.R. China
1 new species hyphomycetes lignicolous freshwater fungi morphology and phylogeny taxonomy Fungi

Abstract

The Nujiang River, an important international river and one of the six major water systems in Yunnan Province, China, is characterized by numerous tributaries, complex topography, and abundant natural resources. During an investigation of lignicolous freshwater fungi in this river, three Neomyrmecridium taxa were isolated from submerged wood samples. Based on morphological characteristics and phylogenetic analyses of combined ITS and LSU sequence data, we introduce a novel species, Neomyrmecridium nujiangense. Additionally, two known species were identified as N. aquaticum and N. fusiforme. This study provides detailed morphological illustrations and phylogenetic trees of both the new and known taxa. Our findings enhance the understanding of freshwater fungal diversity in the Nujiang River and highlight the essential role of integrating morphological and molecular data in fungal taxonomy, particularly for groups exhibiting high phenotypic plasticity.

References

  1. Bao, D.F., Bhat, D.J., Boonmee, S., Hyde, K.D., Luo, Z.L. & Nalumpang, S. (2022) Lignicolous freshwater ascomycetes from Thailand: Introducing Dematipyriforma muriformis sp. nov., one new combination and two new records in Pleurotheciaceae. MycoKeys 93: 57–79. https://doi.org/10.3897/mycokeys.93.87797
  2. Bao, D.F., Hyde, K.D., Maharachchikumbura, S.S.N., Perera, R.H., Thiyagaraja, V., Hongsanan, S., Wanasinghe, D.N., Shen, H.W., Tianm, X.G., Yang, L.Q., Nalumpang, S. & Luo, Z.L. (2023) Taxonomy, phylogeny and evolution of freshwater Hypocreomycetidae (Sordariomycetes). Fungal Diversity 121: 1–94. https://doi.org/10.1007/s13225-023-00521-8
  3. Bao, D.F., Hyde, K.D., McKenzie, E.H., Jeewon, R., Su, H.Y., Nalumpang, S. & Luo, Z.L. (2021) Biodiversity of lignicolous freshwater hyphomycetes from China and Thailand and description of sixteen species. Journal of Fungi 7 (8): 669. https://doi.org/10.3390/jof7080669
  4. Bao, D.F., Luo, Z.L., Liu, J.K., Bhat, D.J., Sarunya, N., Li, W.L., Su, H.Y. & Hyde, K.D. (2018) Lignicolous freshwater fungi in China III: New species and record of Kirschsteiniothelia from northwestern Yunnan Province. Mycosphere 9: 755–768. https://doi.org/10.5943/mycosphere/9/4/4
  5. Bucher, V.V.C., Pointing, S.B., Hyde, K.D. & Reddy, C.A. (2004) Production of wood decay enzymes, loss of mass, and lignin solubilization in wood by diverse tropical freshwater fungi. Microbial Ecology 48 (3): 331–337. https://doi.org/10.1007/s00248-003-0132-x
  6. Calabon, M.S., Hyde, K.D., Jones, E.B.G., Bao, D.F., Bhunjun, C.S., Phukhamsakda, C. & Balasuriya, A. (2023) Freshwater fungal biology. Mycosphere 14 (1): 195–413. https://doi.org/10.5943/mycosphere/14/1/4
  7. Capella-Gutiérrez, S., Silla-Martínez, J.M. & Gabaldón, T. (2009) trimAl: a tool for automated alignment trimming in large-scale phylogenetic analyses. Bioinformatics 25 (15): 1972–1973. https://doi.org/10.1093/bioinformatics/btp348
  8. Cao, B., Phurbu, D., Ralaiveloarisoa, A., Liimatainen, K., Niskanen, T., Ramírez-Cruz, V., Bradshaw, A.J., Dentinger, B.T.M., Ramírez-Guillén, F., Cortés-Pérez, A., Guzmán-Dávalos, L., Villalobos-Arámbula, A.R., Castro-Jauregui, O., da Silva, P.S., Day, R., Davoodian, N., Lebel, T., Castellano, M., May, T.W., Toome, M., Vasey, J., Hofer, K., Thangavel, R., Braithwaite, M., Braithwaite, L., Dutta, A.K., Chattopadhyay, P., Roy, N., Tanti, B., Biswas, P.R., Arumugam, E., Kezo, K., Kaliyaperumal, M., Murugadoss, R., Ji, J.X., Kakishima, M., Cooper, J., Nuytinck, J., Luo, Z.L., Wang, W.P., Zhang, X., Wen, T.C., Jia, A.L., Fan, X.L., Jiang, N., Jiao, N., Zhang, Y., Manawasinghe, I.S., Li, H., Xiong, Y.R., Zhou,T., Tan, Q.L., Hoshino, T., Lagaet, P., De Lange, R., Verbeken, A., Tondeleir, L., Manz, C., Hampe, F., Zhao, C.L., Yang, Y., Zhang, G.Q., Dai, D.Q., Tomšovský, M., Denchev, T.T., Denchev, C.M., Léveillé-Bourret, E., Kemler, M., Chen, Y.P., Maharachchikumbura, S.S.N., Feng, Z.X., Wang, L., Wang, Q.M., Wan, S.P., Yu, F.Q., Liu, J.K., Du, H.Z., Wu, N., Hu, H.L., Su, J.Y., Wang, Z.H., Hu, Y.P., Yu, H., Wang, J., Yang, Y.H., Dong, W., Shu, Y.X., Zhao, H.J., Wang, C.Q., Xia, W.X., Liao, C.F., Doilom, M., Li, G.J., Suwannarach, N., Senwanna, C., Gafforov, Y., Flakus, A., Suchan, T., Plata, O., Rodriguez-Flakus, P., Tamang, J., Acharya, K., Yang, H.D., Jayawardena, R.S., Tiendrebeogo, A., Decock, C., Somda, I., Legrève, A., Yang, Y., Xiao, Y.P., Zhou, X.Z., Li, J.X., Thongklang, N., Han, X.X., Yang, W.Q., Wang, S.H., He, M.Q., Hyde, K.D. & Zhao, R.L. (2025) Fungal diversity notes 1919–2016: taxonomic and phylogenetic contributions to fungal taxa. Fungal Diversity 132: 425–651. https://doi.org/10.1007/s13225-025-00556-z
  9. Chen, Y., Liao, Y., Shami, A., Alharbi, N.K., Liu, J.K. & Maharachchikumbura, S.S. (2025) Advancements in fungal and fungi-like diversity with a new species and updated records. Phytotaxa 701 (2): 127–147. https://doi.org/10.11646/phytotaxa.701.2.1
  10. Crous, P.W., Luangsa-ard, J.J., Wingfield, M.J., Carnegie, A.J., Hernández-Restrepo, M., Lombard, L., Roux, J., Barreto, R.W., Baseia, I.G., Cano-Lira, J.F., Martín, M.P., Morozova, O.V., Stchigel, A.M., Summerell, B.A., Brandrud, T.E., Dima, B., García, D., Giraldo, A., Guarro, J., Gusmão, L.F.P., Khamsuntorn, P., Noordeloos, M.E., Nuankaew, S., Pinruan, U., Rodríguez-Andrade, E., Souza-Motta, C.M., Thangavel, R., van Iperen, A.L., Abreu, V.P., Accioly, T., Alves, J.L., Andrade, J.P., Bahram, M., Baral, H.O., Barbier, E., Barnes, C.W., Bendiksen, E., Bernard, E., Bezerra, J.D.P., Bezerra, J.L., Bizio, E., Blair, J.E., Bulyonkova, T.M., Cabral, T.S., Caiafa, M.V., Cantillo, T., Colmán, A.A., Conceição, L.B., Cruz, S., Cunha, A.O.B., Darveaux, B.A., da Silva, A.L., da Silva, G.A., da Silva, G.M., da Silva, R.M.F., de Oliveira, R.J.V., Oliveira, R.L., De Souza, J.T., Dueñas, M., Evans, H.C., Epifani, F., Felipe, M.T.C., Fernández-López, J., Ferreira, B.W., Figueiredo, C.N., Filippova, N.V., Flores, J.A., Gené, J., Ghorbani, G., Gibertoni, T.B., Glushakova, A.M., Healy, R., Huhndorf, S.M., Iturrieta-González, I., Javan-Nikkhah, M., Juciano, R.F., Jurjević, Ž, Kachalkin, A.V., Keochanpheng, K., Krisai-Greilhuber, I., Li, Y.C., Lima, A.A., Machado, A.R., Madrid, H., Magalhães, O.M.C., Marbach, P.A.S., Melanda, G.C.S., Miller, A.N., Mongkolsamrit, S., Nascimento, R.P., Oliveira, T.G.L., Ordoñez, M.E., Orzes, R., Palma, M.A., Pearce, C.J., Pereira, O.L., Perrone, G., Peterson, S.W., Pham, T.H.G., Piontelli, E., Pordel, A., Quijada, L., Raja, H.A., Rosas de Paz, E., Ryvarden, L., Saitta, A., Salcedo, S.S., Sandoval-Denis, M., Santos, T.A.B., Seifert, K.A., Silva, B.D.B., Smith, M.E., Soares, A.M., Sommai, S., Sousa, J.O., Suetrong, S. & Susca, A. (2018) Fungal Planet description sheets: 785–867. Persoonia 41 (1): 238–417. https://doi.org/10.3767/persoonia.2018.41.12
  11. Crous, P.W., Osieck, E.R., Jurjevi, Ž, Boers, J., Van Iperen, A.L., Starink-Willemse, M., Dima, B., Balashov, S., Bulgakov, T.S., Johnston, P.R., Morozova, O.V., Pinruan, U., Sommai, S., Alvarado, P., Decock, C.A., Lebel, T., McMullan-Fisher, S., Moreno, G., Shivas, R.G., Zhao, L., Abdollahzadeh, J., Abrinbana, M., Ageev, D.V., Akhmetova, G., Alexandrova, A.V., Altés, A., Amaral, A.G.G., Angelini, C., Antonín, V., Arenas, F., Asselman, P., Badali, F., Baghela, A., Bañares, A., Barreto, R.W., Baseia, I.G., Bellanger, J.M., BerrafTebbal, A., Biketova, A.Yu., Bukharova, N.V., Burgess, T.I., Cabero, J., Câmara, M.P.S., Cano-Lira, J.F., Ceryngier, P., Chávez, R., Cowan, D.A., de Lima, A.F., Oliveira, R.L., Denman, S., Dang, Q.N., Dovana, F., Duarte, I.G., Eichmeier, A., Erhard, A., Esteve-Raventós, F., Fellin, A., Ferisin, G., Ferreira, R.J., Ferrer, A., Finy, P., Gaya, E., Geering, A.D.W., Gil-Durán, C., Glässnerová, K., Glushakova, A.M., Gramaje, D., Guard, F.E., Guarnizo, A.L., Haelewaters, D., Halling, R.E., Hill, R., Hirooka, Y., Hubka, V., Iliushin, V.A., Ivanova, D.D., Ivanushkina, N.E., Jangsantear, P., Justo, A., Kachalkin, A.V., Kato, S., Khamsuntorn, P., Kirtsideli, I.Y., Knapp, D.G., Kochkina, G.A., Koukol, O., Kovács, G.M., Kruse, J., Kumar, T.K.A., Kušan, I., Læssøe, T., Larsson, E., Lebeuf, R., Levicán, G., Loizides, M., Marinho, P., Luangsa-ard, J.J., Lukina, E.G., Magaña-Dueñas, V., Maggs-Kölling, G., Malysheva, E.F., Malysheva, V.F., Martín, B., Martín, M.P., Matočec, N., McTaggart, A.R., Mehrabi-Koushki, M., Mešić, A., Miller, A.N., Mironova, P., Moreau, P.A., Morte, A., Müller, K., Nagy, L.G., Nanu, S., Navarro-Ródenas, A., Nel, W.J., Nguyen, T.H., Nóbrega, T.F., Noordeloos, M.E., Olariaga, I., Overton, B.E., Ozerskaya, S.M., Palani, P., Pancorbo, F., Papp, V., Pawłowska, J., Pham, T.Q., Phosri, C., Popov, E.S., Portugal, A., Pošta, A., Reschke, K., Reul, M., Ricci, G.M., Rodríguez, A., Romanowski, J., Ruchikachorn, N., Saar, I., Safi, A., Sakolrak, B., Salzmann, F., Sandoval-Denis, M., Sangwichein, E., Sanhueza, L., Sato, T., Sastoque, A., Senn-Irlet, B., Shibata, A., Siepe, K., Somrithipol, S., Spetik, M., Sridhar, P., Stchigel, A.M., Stuskova, K., Suwannasai, N., Tan, Y.P., Thangavel, R., Tiago, I., Tiwari, S., Tkalčec, Z., Tomashevskaya, M.A., Tonegawa, C., Tran, H.X., Tran, N.T., Trovão, J., Trubitsyn, V.E., Van Wyk, J., Vieira, W.A.S., Vila, J., Visagie, C.M., Vizzini, A., Volobuev, S.V., Vu, D.T., Wangsawat, N., Yaguchi, T., Ercole, E., Ferreira, B.W., de Souza, A.P., Vieira, B.S. & Groenewald, J.Z. (2021) Fungal Planet description sheets: 1284–1382. Persoonia 47 (1): 178–374. https://doi.org/10.3767/persoonia.2021.47.06
  12. Dong, W., Wang, B., Hyde, K.D., McKenzie, E.H.C., Raja, H.A., Tanaka, K., Abdel-Wahab, M.A., Abdel-Aziz, F.A., Doilom, M., Phookamsak, R., Hongsanan, S., Wanasinghe, D.N., Yu, X.D., Wang, G.N., Yang, H., Yang, J., Thambugala, K.M., Tian, Q., Luo, Z.L., Yang, J.B., Miller, A.N., Fournier, J., Boonmee, S., Hu, D.M. & Nalumpang, S. (2020) Freshwater Dothideomycetes. Fungal Diversity 105 (1): 319–575. https://doi.org/10.1007/s13225-020-00463-5
  13. Duarte, S., Fernandes, I., Nogueira, M.J., Cássio, F. & Pascoal, C. (2013) Temperature alters interspecific relationships among aquatic fungi. Fungal Ecology 6 (3): 187–191. https://doi.org/10.1016/j.funeco.2013.02.001
  14. Hall, T.A. (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 41: 95–98.
  15. Hyde, K.D., Dong, Y., Phookamsak, R., Jeewon, R., Bhat, D.J., Jones, E.B.G., Liu, N.G., Abeywickrama, P.D., Mapook, A., Wei, D., Perera, R.H., Manawasinghe, I.S., Pem, D., Bundhun, D., Karunarathna, A., Ekanayaka, A.H., Bao, D.F., Li, J., Samarakoon, M.C., Chaiwan, N., Lin, C.G., Phutthacharoen, K., Zhang, S.N., Senanayake, I.C., Goonasekara, I.D., Thambugala, K.M., Phukhamsakda, C., Tennakoon, D.S., Jiang, H.B., Yang, J., Zeng, M., Huanraluek, N. & Liu, J.K. (Jack), Wijesinghe, S.N., Tian, Q., Tibpromma, S., Brahmanage, R.S., Boonmee, S., Huang, S.K., Thiyagaraja, V., Lu, Y.Z., Jayawardena, R.S., Dong, W., Yang, E.F., Singh, S.K., Singh, S.M., Rana, S., Lad, S.S., Anand, G., Devadatha, B., Niranjan, M., Sarma, V.V., Liimatainen, K., Aguirre-Hudson, B., Niskanen, T., Overall, A., Alvarenga, R.L.M., Gibertoni, T.B. & Pfliegler, W.P., Horváth, E., Imre, A., Alves, A.L., Santos, A.C. da S., Tiago, P.V., Bulgakov, T.S., Wanasinghe, D.N., Bahkali, A.H., Doilom, M., Elgorban, A.M., Maharachchikumbura, S.S.N., Rajeshkumar, K.C., Haelewaters, D., Mortimer, P.E., Zhao, Q., Lumyong, S., Xu, J. & Sheng, J. (2020) Fungal diversity notes 1151–1276: taxonomic and phylogenetic contributions on genera and species of fungal taxa. Fungal Diversity 100 (1): 5–277. https://doi.org/10.1007/s13225-020-00439-5
  16. Hyde, K.D., Fryar, S., Tian, Q. & Bahkali, A.H. (2016) Lignicolous freshwater fungi along a northsouth latitudinal gradient in the Asian/Australian region; can we predict the impact of global warming on biodiversity and function? Fungal Ecology 19: 190–200. https://doi.org/10.1016/j.funeco.2015.07.002
  17. Hyde, K.D., Noorabadi, M.T., Thiyagaraja, V., He, M.Q., Johnston, P.R., Wijesinghe, S.N., Armand, A., Biketova, A.Y., Chethana, K.W.T., Erdoğdu, M., Ge, Z.W., Groenewald, J.Z., Hongsanan, S., Kušan, I., Leontyev, D.V., Li, D.W., Lin, C.G., Liu, N.G., Maharachchikumbura, S.S.N., Matočec, N., May, T.W., McKenzie, E.H.C., Mešić, A., Perera, R.H., Phukhamsakda, C., Piątek, M., Samarakoon, M.C., Selcuk, F., Senanayake, I.C., Tanney, J.B., Tian, Q., Vizzini, A., Wanasinghe, D.N., Wannasawang, N., Wijayawardene, N.N., Zhao, R.L., Abdel-Wahab, M.A., Abdollahzadeh, J., Abeywickrama, P.D., Abhinav, Absalan, S., Acharya, K., Afshari, N., Afshan, N.S., Afzalinia, S., Ahmadpour, S.A., Akulov, O., Alizadeh, A., Alizadeh, M., Al-Sadi, A.M., Alves, A., Alves, V.C.S., Alves-Silva, G., Antonín, V., Aouali, S., Aptroot, A., Apurillo, C.C.S., Arias, R.M., Asgari, B., Asghari, R., Assis, D.M.A., Assyov, B., Atienza, V., Aumentado, H.D.R., Avasthi, S., Azevedo, E., Bakhshi, M., Bao, D.F., Baral, H.O., Barata, M., Barbosa, K.D., Barbosa, R.N., Barbosa, F.R., Baroncelli, R., Barreto, G.G., Baschien, C., Bennett, R.M., Bera, I., Bezerra, J.D.P., Bhunjun, C.S., Bianchinotti, M.V., Błaszkowski, J., Boekhout, T., Bonito, G.M., Boonmee, S., Boonyuen, N., Bortnikov, F.M., Bregant, C., Bundhun, D., Burgaud, G., Buyck, B., Caeiro, M.F., Cabarroi-Hernández, M., Cai, M.F., Cai, L., Calabon, M.S., Calaça, F.J.S., Callalli, M., Câmara, M.P.S., Cano-Lira, J., Cao, B., Carlavilla, J.R., Carvalho, A., Carvalho, T.G., Castañeda-Ruiz, R.F., Catania, M.D.V., Cazabonne, J., Cedeño-Sanchez, M., Chaharmiri-Dokhaharani, S., Chaiwan, N., Chakraborty, N., Cheewankoon, R., Chen, C., Chen, J., Chen, Q., Chen, Y.P., Chinaglia, S., Coelho-Nascimento, C.C., Coleine, C., Costa-Rezende, D.H., Cortés-Pérez, A., Crouch, J.A., Crous, P.W., Cruz, R.H.S.F., Czachura, P., Damm, U., Darmostuk, V., Daroodi, Z., Das, K., Das, K., Davoodian, N., Davydov, E.A., da Silva, G.A., da Silva, I.R., da Silva, R.M.F., da Silva Santos, A.C., Dai, D.Q., Dai, Y.C., de Groot, M.D., De Kesel, A., De Lange, R., de Medeiros, E.V., de Souza, C.F.A., de Souza, F.A., dela Cruz, T.E.E., Decock, C., Delgado, G., Denchev, C.M., Denchev, T.T., Deng, Y.L., Dentinger, B.T.M., Devadatha, B., Dianese, J.C., Dima, B., Doilom, M., Dissanayake, A.J., Dissanayake, D.M.L.S., Dissanayake, L.S., Diniz, A.G., Dolatabadi, S., Dong, J.H., Dong, W., Dong, Z.Y., Drechsler-Santos, E.R., Druzhinina, I.S., Du, T.Y., Dubey, M.K., Dutta, A.K., Elliott, T.F., Elshahed, M.S., Egidi, E., Eisvand, P., Fan, L., Fan, X., Fan, X.L., Fedosova, A.G., Ferro, L.O., Fiuza, P.O., Flakus, A., Fonseca, E.O., Fryar, S.C., Gabaldón, T., Gajanayake, A.J., Gannibal, P.B., Gao, F., García-Sánchez, D., García-Sandoval, R., Garrido-Benavent, I., Garzoli, L., Gasca-Pineda, J., Gautam, A.K., Gené, J., GhobadNejhad,M., Ghosh, A., Giachini, A.J., Gibertoni, T.B., Gentekaki, E., Gmoshinskiy, V.I., Góes-Neto, A., Gomdola, D., Gorjón, S.P., Goto, B.T., Granados-Montero, M.M., Griffith, G.W., Groenewald, M., Grossart, H.-P., Gu, Z.R., Gueidan, C., Gunarathne, A., Gunaseelan, S., Guo, S.L., Gusmão, L.F.P., Gutierrez, A.C., Guzmán-Dávalos, L., Haelewaters, D., Haituk, H., Halling, R.E., He, S.C., Heredia, G., Hernández-Restrepo, M., Hosoya, T., Hoog, S.D., Horak, E., Hou, C.L., Houbraken, J., Htet, Z.H., Huang, S.K., Huang, W.J., Hurdeal, V.G., Hustad, V.P., Inácio, C.A., Janik, P., Jayalal, R.G.U., Jayasiri, S.C., Jayawardena, R.S., Jeewon, R., Jerônimo, G.H., Jin, J., Jones, E.B.G., Joshi, Y., Jurjević, Ž. & Justo, A. (2024) The 2024 Outline of Fungi and fungus-like taxa. Mycosphere 15: 5146–6239. https://doi.org/10.5943/mycosphere/15/1/25
  18. Hyde, K.D., Jones, E.B.G., Liu, J.K., Ariyawansa, H., Boehm, E., Boonmee, S., Braun, U., Chomnunti, P., Crous, P.W., Dai, D.Q., Diederich, P., Dissanayake, A., Doilom, M., Doveri, F., Hongsanan, S., Jayawardena, R., Lawrey, J.D., Li, Y.M., Liu, Y.X., Lücking, R., Monkai, J., Muggia, L., Nelsen, M.P., Pang, K.L., Phookamsak, R., Senanayake, I.C., Shearer, C.A., Suetrong, S., Tanaka, K., Thambugala, K.M., Wijayawardene, N.N., Wikee, S., Wu, H.X. & Zhang, Y. (2013) Families of Dothideomycetes. Fungal Diversity 63 (1): 1–313. https://doi.org/10.1007/s13225-013-0263-4
  19. Katoh, K. & Standley, D.M. (2013) MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Molecular biology and evolution 30 (4): 772–780. https://doi.org/10.1093/molbev/mst010
  20. Li, L.L., Shen, H.W., Bao, D.F., Wanasinghe, D.N., Lu, Y.Z., Feng, Y. & Luo, Z.L. (2022) The plethora of Tubeufiaceae in lakes of the northwestern Yunnan plateau, China. Frontiers in Microbiology 13: 1056669. https://doi.org/10.3389/fmicb.2022.1056669
  21. Liu, X.Y., Udayanga, D., Luo, Z.L., Chen, L.J., Zhou, D.Q., Su, H.Y. & Hyde, K.D. (2015) Backbone tree for Chaetothyriales with four new species of Minimelanolocus from aquatic habitats. Fungal biology 119 (11): 1046–1062. https://doi.org/10.1016/j.funbio.2015.08.005
  22. Luo, J., Yin, J., Cai, L., Zhang, K. & Hyde, K.D. (2004) Freshwater fungi in Lake Dianchi, a heavily polluted lake in Yunnan, China. Fungal diversity.
  23. Luo, Z.L., Bhat, D.J., Jeewon, R., Boonmee, S., Bao, D.F., Zhao, Y.C., Chai, H.M., Su, H.Y. & Su, X.J. (2017) Molecular phylogeny and morphological characterization of asexual fungi (Tubeufiaceae) from freshwater habitats in Yunnan, China. Cryptogamie, Mycologie 38 (1): 27–53. https://doi.org/10.7872/crym/v38.iss1.2017.27
  24. Luo, Z.L., Hyde, K.D., Liu, J.K., Bhat, D.J., Bao, D.F., Li, W.L., Su, X.J., Yang, X.Y. & Su, H.Y. (2018) Lignicolous freshwater fungi from China II: novel Distoseptispora (Distoseptisporaceae) species from northwestern Yunnan Province and a suggested unified method for studying lignicolous freshwater fungi. Mycosphere 9: 444–461. https://doi.org/10.5943/mycosphere/9/3/2
  25. Luo, Z.L., Hyde, K.D., Liu, J.K., Maharachchikumbura, S.S.N., Jeewon, R., Bao, D.F., Bhat, D.J., Lin, C.G., Li, W.L. & Yang, J. (2019) Freshwater Sordariomycetes. Fungal Diversity 99: 451–660. https://doi.org/10.1007/s13225-019-00438-1
  26. Maharachchikumbura, S.S., Hyde, K.D., Jones, E.B.G., McKenzie, E.H.C., Huang, S.K., Abdel-Wahab, M.A., Daranagama, D.A., Dayarathne, M., D’souza, M.J., Goonasekara, I.D., Hongsanan, S., Jayawardena, R.S., Kirk, P.M., Konta, S., Liu, J.K., Liu, Z.Y., Norphanphoun, C., Pang, K.L., Perera, R.H., Senanayake, I.C., Shang, Q., Shenoy, B.D., Xiao, Y., Bahkali, A.H., Kang, J., Somrothipol, S. & Suetrong, S., Wen, T. & Xu, J. (2015) Towards a natural classification and backbone tree for Sordariomycetes. Fungal diversity 72 (1): 199–301. https://doi.org/10.1007/s13225-015-0331-z
  27. Miller, M.A., Pfeiffer, W. & Schwartz, T. (2010) Creating the CIPRES science gateway for inference of large phylogenetic trees. In: Proceedings of the Gateway Computing Environments Workshop (GCE). New Orleans, pp. 1–8. https://doi.org/10.1109/GCE.2010.5676129
  28. Ranala, B. & Yang, Z. (1996) Probability distribution of molecular evolutionary trees: a new method of phylogenetic inference. Journal of Molecular Evolution 43: 304–311. https://doi.org/10.1007/BF02338839
  29. Ronquist, F., Teslenko, M., Van Der Mark, P., Ayres, D.L., Darling, A., Höhna, S. & Huelsenbeck, J.P. (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic biology 61 (3): 539–542. https://doi.org/10.1093/sysbio/sys029
  30. Senanayake, I.C., Rathnayaka, A.R., Marasinghe, D.S., Calabon, M.S., Gentekaki, E., Lee, H.B. & Xiang, M.M. (2020) Morphological approaches in studying fungi: Collection, examination, isolation, sporulation and preservation. Mycosphere 11 (1): 2678–2754. https://doi.org/10.5943/mycosphere/11/1/20
  31. Serrano, L., Sosa, D., Magdama, F., Espinoza, F., Quevedo, A., Vera, M., Villavicencio, M., Maridueña, G., Pérez-Martinez, S., Malosso, E., Ramos-García, B. & Castañeda-Ruiz, R.F. (2020) Neomyrmecridium asymmetricum sp. nov. from Ecuador. Mycotaxon 135 (1): 151–165. https://doi.org/10.5248/131.151
  32. Shen, H.W., Bao, D.F., Bhat, D.J., Su, H.Y. & Luo, Z.L. (2022) Lignicolous freshwater fungi in Yunnan Province, China: an overview. Mycology 13: 119–132. https://doi.org/10.1080/21501203.2022.2058638
  33. Shen, H.W., Bao, D.F., Boonmee, S., Lu, Y.Z., Su, X.J., Li, Y.X. & Luo, Z.L. (2024) Diversity of Distoseptispora (Distoseptisporaceae) taxa on submerged decaying wood from the Red River in Yunnan, China. MycoKeys 102: 1–28. https://doi.org/10.3897/mycokeys.102.116096
  34. Shen, H.W., Bao, D.F., Boonmee, S., Su, X.J., Tian, X.G., Hyde, K.D. & Luo, Z.L. (2023) Lignicolous freshwater fungi from plateau lakes in China (I): morphological and phylogenetic analyses reveal eight species of Lentitheciaceae, including new genus, new species and new records. Journal of Fungi 9 (10): 962. https://doi.org/10.3390/jof9100962
  35. Stamatakis, A. (2006) RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics 22: 2688–2690. https://doi.org/10.1093/bioinformatics/btl446
  36. Stamatakis, A., Hoover, P. & Rougemont, J. (2008) A rapid bootstrap algorithm for the RAxML web-servers. Systematic Biology 75: 758–771. https://doi.org/10.1080/10635150802429642
  37. Swe, A., Jeewon, R., Pointing, S.B. & Hyde, K.D. (2009) Diversity and abundance of nematode-trapping fungi from decaying litter in terrestrial, freshwater and mangrove habitats. Biodiversity and Conservation 18 (6): 1695–1714. https://doi.org/10.1007/s10531-008-9553-7
  38. Tian, Q., Doilom, M., Luo, Z.L., Chomnunti, P., Bhat, J.D., Xu, J.C. & Hyde, K.D. (2016) Introducing Melanoctona tectonae gen. et sp. nov. and Minimelanolocus yunnanensis sp. nov. (Herpotrichiellaceae, Chaetothyriales). Cryptogamie, Mycologie 37 (4): 477–492. https://doi.org/10.7872/crym/v37.iss4.2016.477
  39. Vaidya, G., Lohman, D.J. & Meier, R. (2011) SequenceMatrix: concatenation software for the fast assembly of multi‐gene datasets with character set and codon information. Cladistics 27 (2): 171–180. https://doi.org/10.1111/j.1096-0031.2010.00329.x
  40. Vilgalys, R. & Hester, M. (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. Journal of Bacteriology 172 (8): 4238–4246. https://doi.org/10.1128/jb.172.8.4238-4246.1990
  41. Wang, G.N., Yu, X.D., Dong, W., Bhat, D.J., Boonmee, S., Zhang, D. & Zhang, H. (2019) Freshwater hyphomycetes in Eurotiomycetes: a new species of Minimelanolocus and a new collection of Thysanorea papuana (Herpotrichiellaceae). Mycological Progress 18: 511–522. https://doi.org/10.1007/s11557-019-01473-7
  42. White, T.J., Bruns, T., Lee, S. & Taylor, J.W. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR Protocols: A Guide to Methods and Applications 18: 315–322. https://doi.org/10.1016/b978-0-12-372180-8.50042-1
  43. Wijayawardene, N.N., Crous, P.W., Kirk, P.M., Hawksworth, D.L., Boonmee, S., Braun, U., Dai, D.Q., D’souza, M.J., Diederich, P., Dissanayake, A., Doilom, M., Hongsanan, S., Jones, E.B.G., Groenewald, J.Z., Jayawardena, R., Lawrey, J.D., Liu, J.K., Lücking, R., Madrid, H., Manamgoda, D.S., Muggia, L., Nelsen, M.P., Phookamsak, R., Suetrong, S., Tanaka, K. & Thambugala, K.M., Wanasinghe, D.N., Wikee, S., Zhang, Y., Aptroot, A., Ariyawansa, H.A., Bahkali, A.H., Bhat, D.J., Gueidan, C., Chomnunti, P., De Hoog, G.S., Knudsen, K., Li, W.J., McKenzie, E.H.C., Miller, A.N., Phillips, A.J.L., Piątek, M., Raja, H.A., Shivas, R.S., Slippers, B., Taylor, J.E., Tian, Q., Wang, Y. & Woudenberg, J.H.C., Cai, L., Jaklitsch, W.M. & Hyde, K.D. (2014) Naming and outline of Dothideomycetes–2014 including proposals for the protection or suppression of generic names. Fungal Diversity 69 (1): 1–55. https://doi.org/10.1007/s13225-014-0309-2
  44. Wong, M.K.M., Goh, T.K., Hodgkiss, I.J., Hyde, K.D., Ranghoo, V.M., Tsui, C.K.M., Ho, W.H., Wong, W.S.W. & Yuen, T.K. (1998) Role of fungi in freshwater ecosystems. Biodiversity & Conservation 7 (9): 1187–1206. https://doi.org/10.1023/A:1008883716975
  45. Xu, R.J., Dong, W., Wei, D.P., Zhao, Q. & Boonmee, S. (2023) Two new species in Neomyrmecridium and two new records in Myrmecridium (Myrmecridiaceae, Myrmecridiales) from the Tibetan Plateau, China. Current Research in Environmental & Applied Mycology 13: 489–504. https://doi.org/10.5943/cream/13/1/18
  46. Yang, J., Liu, L.L., Jones, E.B.G., Hyde, K.D., Liu, Z.Y., Bao, D.F., Liu, N.G., Li, W.L., Shen, H.W., Yu, X.D. & Liu, J.K. (2023) Freshwater fungi from karst landscapes in China and Thailand. Fungal Diversity 119 (1): 1–212. https://doi.org/10.1007/s13225-023-00514-7
  47. Yang, L., Bao, D.F., Luo, Z.L., Su, X.J. & Su, H.Y. (2022) Neoascotaiwania aquatica sp. nov. from a freshwater habitat in Yunnan Province, China. Phytotaxa 531 (2): 120–128. https://doi.org/10.11646/phytotaxa.531.2.4
  48. Zare-Maivan, H. & Shearer, C.A. (1988) Extracellular enzyme production and cell wall degradation by freshwater lignicolous fungi. Mycologia 80 (3): 365–375. https://doi.org/10.1080/00275514.1988.12025551
  49. Zhang, J.Y., Hyde, K.D., Bao, D.F., Hongsanan, S., Liu, Y.X., Kang, J.C., Luo, Z.L., Liu, J.K. & Lu, Y.Z. (2025) A worldwide checklist and morpho-molecular systematics of fungi associated with pteridophytes. Fungal Diversity 132: 151–423. https://doi.org/10.1007/s13225-025-00554-1
  50. Zhang, L., Bao, D.F., Shen, H.W. & Luo, Z.L. (2024) Diversity of lignicolous freshwater fungi from Yuanjiang River in Yunnan (China), with the description of four new species. Journal of Fungi 10 (12): 881. https://doi.org/10.3390/jof10120881
  51. Zhang, Z.P., Yao, C.Y., Wu, L., Luo, Z.L., Sun, G., Guo, Z.Y., Li, X.S., Lin, F. & Chen, X.Y. (2024) Fish diversity and threat factors in the Yunnan section of the Nujiang River. Biodiversity Science 32 (7): 24076. https://doi.org/10.17520/biods.2024076

How to Cite

Ma, X.-L., Shen, H.-W., Wang, W.-P. & Luo, Z.-L. (2026) Morpho-phylogenetic evidence reveals a new species and two known taxa of Neomyrmecridium from freshwater habitats in the Nujiang River, Yunnan, China. Phytotaxa 740 (2): 138–152. https://doi.org/10.11646/phytotaxa.740.2.2