Skip to main content Skip to main navigation menu Skip to site footer
Responsive image
Issue: Vol. 739 No. 2: 28 Jan. 2026
Type: Article
Published: 2026-01-28
DOI: 10.11646/phytotaxa.739.2.2
Page range: 134-144
Abstract views: 26
PDF downloaded: 0

Entoloma fissuromarginatum (Entolomataceae, Agaricales), a new species from Battagram District, Pakistan

State Key Laboratory of Green Pesticide, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang 550025, P.R. China; Engineering Research Center of Southwest Bio-Pharmaceutical Resources, Ministry of Education, Guizhou University, Guiyang 550025, P.R. China; School of Pharmacy, Guizhou University, Guiyang 550025, P.R. China
Department of Botany, Division of Science & Technology, University of Education, 54500, Lahore, Pakistan
Department of Botany, Hazara University, Mansehra, Pakistan
Department of Botany, Division of Science & Technology, University of Education, 54500, Lahore, Pakistan
Center for Yunnan Plateau Biological Resources Protection and Utilization & Yunnan International Joint Laboratory of Fungal Sustainable Utilization in South and Southeast Asia, College of Biology and Food Engineering, Qujing Normal University, Qujing 655099, China
Department of Botany, Hazara University, Mansehra, Pakistan
State Key Laboratory of Green Pesticide, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang 550025, P.R. China; Engineering Research Center of Southwest Bio-Pharmaceutical Resources, Ministry of Education, Guizhou University, Guiyang 550025, P.R. China; School of Pharmacy, Guizhou University, Guiyang 550025, P.R. China; Department of Plant Pathology, College of Agriculture, Guizhou University, Guiyang 550025, P.R. China
Basidiomycota Khyber Pakhtunkhwa new species phylogeny taxonomy Fungi

Abstract

A new species, Entoloma fissuromarginatum, is described from the Battagram District, Khyber Pakhtunkhwa Province, Pakistan. The species is delineated based on the combination of morphological characteristics and molecular evidence, with nrITS sequence data supporting its distinct taxonomic status. Entoloma fissuromarginatum is characterized by a hemispherical to plano-concave and light brownish-gray pileus with split margins, adnate and light brownish-gray lamellae, reddish brown and scaly stipe, heterodiametric basidiospores (average 6.40 × 5.61 μm) having 5–6 angles, absence of pleurocystidia, lageniform cheilocystidia, narrowly clavate to subcapitate caulocystidia, and a cutis pileipellis and stipitipellis. A description, phylogenetic analysis results, an illustration, and a comparison with closely related taxa of the new species are provided.

References

  1. Aman, N., Khalid, A.N. & Moncalvo, J.M. (2022) A compendium of macrofungi of Pakistan by ecoregions. MycoKeys 89: 171. https://doi.org/10.3897/mycokeys.89.81148
  2. Asif, M., Saba, M., Akram, W., Maula, F. & Raza, M. (2024) Integrated morphological and molecular approaches unveil a new species and a new record of the genus Entoloma (Entolomataceae) from southern Punjab, Pakistan. Phytotaxa 653 (3): 219–240. https://doi.org/10.11646/phytotaxa.653.3.3
  3. Brandrud, T.E., Bendiksen, E., Jordal, J.B., Weholt, Ø., Eidissen, S.E., Lorås, J.A., Dima, B. & Noordeloos, M.E. (2018) Entoloma species of the rhodopolioid clade (subgenus Entoloma; Tricholomatinae, Basidiomycota) in Norway. Agarica 38: 21–46. https://doi.org/10.5617/agarica.11643
  4. Co-David, D.L.V., Langeveld, D. & Noordeloos, M.E. (2009) Molecular phylogeny and spore evolution of Entolomataceae. Persoonia 23: 147–176. https://doi.org/10.3767/003158509X480944
  5. Díaz, J.A. & Rodríguez, A.R. (2018) Cita de Entoloma parasiticum en la Reserva de la Biosfera de “Os Ancares Lucenses e Montes de Cervantes, Navia e Becerreá” (Lugo-España). Tarrelos (Revista de la Federación galega de Micoloxía) 20: 14–17.
  6. Dima, B., Brandrud, T.E., Corriol, G., Jansen, G.M., Jordal, J.B., Khalid, A.N., Larsson, E., Lorås, J., Morozova, V., Naseer, A., Noordeloos, M.E., Rossi, W., Santamaria, S., Sarwar, S., Sesli, E., Usman, M., Afshan, N.S., Ahmad, I., Banerjee, A., Banerjee, K., Bendiksen, E., Rodrigues, Da Silva, Colombo, D., De Kesel, A., Dovana, F., Ferisin, G., Hussain, S., Islam, S., Jesus, A.L., Kaygusuz, O., Krisai-Greilhuber, I., Mahammad, S., Mishra, D.K., Nath, P.S., De, Oliveira, Da Paixã, S.C., Panja, B., Papp, V., Amorim, P. & Zottarelli, C.L. (2021) FUSE 7. Sydowia 73: 271–340. https://doi.org/10.12905/0380.sydowia73-2021-0271
  7. Gardes, M. & Bruns, T.D. (1993) ITS primers with enhanced specificity for basidiomycetes—application to the identification of mycorrhizae and rusts. Molecular Ecology 2: 113–118. https://doi.org/10.1111/j.1365-294X.1993.tb00005.x
  8. Gates, G.M. & Noordeloos, M. (2007) Preliminary studies in the genus Entoloma in Tasmania. Persoonia 19: 157–226.
  9. Gates, G.M., Horton, B.M. & Noordeloos, M.E. (2009) A new Entoloma (Basidiomycetes, Agaricales) from Tasmania. Mycotaxon 107: 175–179. https://doi.org/10.5248/107.175
  10. Hall, T.A. (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 45: 95–98.
  11. He, X.L., Horak, E., DiWang, T.H.L., Peng, W.H. & Gan, B.C. (2019) Descriptions of five new species in Entoloma subgenus Claudopus from China, with molecular phylogeny of Entoloma sl. MycoKeys 61: 1. https://doi.org/10.3897/mycokeys.61.46446
  12. He, X.L., Li, T.H., Jiang, Z.D. & Shen, Y.H. (2012) Four new species of Entoloma s.l. (Agaricales) from southern China. Mycological Progress 11 (4): 915–925. https://doi.org/10.1007/s11557-012-0807-0
  13. Hu, Y., Karunarathna, S.C., Li, H., Galappaththi, M.C., Zhao, C.L., Kakumyan, P. & Mortimer, P.E. (2022) The impact of drying temperature on basidiospore size. Diversity 14 (4): 239. https://doi.org/10.3390/d14040239
  14. Hofstetter, V., Clémençon, H., Vilgalys, R. & Moncalvo, J.M. (2002) Phylogenetic analyses of the Lyophylleae (Agaricales, Basidiomycota) based on nuclear and mitochondrial rDNA sequences. Mycological Research 106 (9): 1043–1059. https://doi.org/10.1017/S095375620200641X
  15. Kasuya, T., Takehashi, S., Hoshino, T. & Noordeloos, M.E. (2010) Entoloma aprile (Agaricales, Entolomataceae) new to Japan, with notes on its mycorrhiza associated with Populus maximowiczii in cool-temperate deciduous forests of Hokkaido. Sydowia 62 (2): 205–223.
  16. Khalid, A.N., Razzaq, F., Naseer, A., Qasim, T. & Kanwal, S. (2024) Two new species and a new record of Entolomatoid fungi (Entolomataceae, Agaricales) from Pakistan. Phytotaxa 666 (1): 1–16. https://doi.org/10.11646/phytotaxa.666.1.1
  17. Koba-yashi, H. & Hatano, K. (2001) A morphological study of the mycorrhiza of Entoloma clypeatum f. hybridum on Rosamultiflora. Mycoscience 42: 83–90. https://doi.org/10.1007/BF02463979
  18. Kokkonen, K. (2015) A survey of boreal Entoloma with emphasis on the subgenus Rhodopolia. Mycological Progress 14 (12): 116. https://doi.org/10.1007/s11557-015-1135-y
  19. Kondo, K., Nakamura, K., Ishigaki, T., Sakata, K., Obitsu, S., Noguchi, A., Fukuda, N., Nagasawa, E., Teshima, R. & Nishimaki-Mogami, T. (2017) Molecular phylogenetic analysis of new Entoloma rhodopolium-related species in Japan and its identification method using PCR-RFLP. Scientific Reports 7: 1–12. https://doi.org/10.1038/s41598-017-14466-x
  20. Kondo, K., Nakamura, K., Ishigaki, T., Sakata, K., Obitsu, S., Noguchi, A., Fukuda, N., Nagasawa, E., Teshima, R. & Nishimaki-Mogami, T. (2017) Molecular phylogenetic analysis of new Entoloma rhodopolium-related species in Japan and its identification method using PCR-RFLP. Scientific Reports 7 (1): 14942. https://doi.org/10.1038/s41598-017-14466-x
  21. Largent, D.L. (1994) Entolomatoid fungi of the western United States and Alaska. Mad River Press, Eureka, California.
  22. Largent, D.L., Bergemann, S.E. & Abell-Davis, S.E. (2015) Entoloma species from New South Wales and northeastern Queensland, Australia. Mycotaxon 129 (2): 329–359. https://doi.org/10.5248/129.329
  23. Morgado, L.N., Noordeloos, M.E., Lamoureux, Y. & Geml, J. (2013) Multi-gene phylogenetic analyses reveal species limits, phylogeographic patterns, and evolutionary histories of key morphological traits in Entoloma (Agaricales, Basidiomycota). Persoonia-Molecular Phylogeny and Evolution of Fungi 31 (1): 159–178. https://doi.org/10.3767/003158513X673521
  24. Munsell (1975) Munsell soil color charts. Macbeth Division of Kollmorgen Corporation, Baltimore, Maryland.
  25. Noordeloos, M.E. & Hausknecht, A. (2007) The genus Entoloma (Basidiomycetes, Agaricales) of the Mascarenes and Seychelles. Fungal Diversity 27: 111–144.
  26. Noordeloos, M.E. & Morozova, O.V. (2010) New and noteworthy Entoloma species from the Primorsky Territory, Russian Far East. Mycotaxon 112 (1): 231–255. https://doi.org/10.5248/112.231
  27. Noordeloos, M.E. (1981) Introduction to the taxonomy of the genus Entoloma sensu lato (Agaricales). Persoonia 11: 121–151.
  28. Noordeloos, M.E. (1992) Entoloma s.l. In: Candusso, M. (ed.) Funghi Europaei, vol 5. Giovanna Biella, Saronno, pp. 1–760.
  29. Noordeloos, M.E. (2004) Entoloma s.l. In: Fungi Europaei 5a. pp. 761–1378.
  30. Noordeloos, M.E., Weholt, Ø., Bendiksen, E., Brandrud, T.E., Eidissen, S.E., Lorås, J.A., Morozova, O. & Dima, B. (2018) Entoloma aurorae-borealis sp. nov. and three rare Entoloma species in the Sinuatum clade (subg. Entoloma) from northern Europe. Sydowia 70: 199–210. https://doi.org/10.12905/0380.sydowia70-2018-0199
  31. Pegler, D.N. (1977) Entolomataceae (Agaricales) from India & Sri Lanka. Kew Bulletin 32: 189–220. https://doi.org/10.2307/4117266
  32. Persoon, C.H. (1801) Synopsis methodica fungorum: 1–706.
  33. Rambaut, A. (2014) FigTree v1.4.2. Available from: http://tree.bio.ed.ac.uk/software/figtree (accessed 25 May 2025)
  34. Rathnayaka, A.R., Tennakoon, D.S., Jones, G.E., Wanasinghe, D.N., Bhat, D.J., Priyashantha, A.H., Stephenson, S.L., Tibpromma, S. & Karunarathna, S.C. (2025) Significance of precise documentation of hosts and geospatial data of fungal collections, with an emphasis on plant-associated fungi. New Zealand Journal of Botany 63 (2–3): 462–489. https://doi.org/10.1080/0028825X.2024.2381734
  35. Reschke, K., Noordeloos, M.E., Manz, C., Hofmann, T.A., Rodríguez-Cedeño, J., Dima, B. & Piepenbring, M. (2022) Fungal diversity in the tropics: Entoloma spp. in Panama. Mycological Progress 21 (1): 93–145. https://doi.org/10.1007/s11557-021-01752-2
  36. Ronquist, F., Teslenko, M., van der Mark, P., Ayres, D.L., Darling, A., Höhna, S., Larget, B., Liu, L., Suchard, M.A. & Huelsenbeck, J.P. (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Systematics Biology 61: 539–542. https://doi.org/10.1093/sysbio/sys029
  37. Sana, Kiran, M., Okabe, S., Hafazallah, K., Javeed, P. & Ali, W. (2025) Inocybe alboaurantiaca (Inocybaceae), a new species from Asia. Nordic Journal of Botany e04914. https://doi.org/10.1002/njb.04914
  38. Trifinopoulos, J., Nguyen, L.T., von Haeseler, A. & Minh, B.Q. (2016) W-IQ-TREE: a fast online phylogenetic tool for maximum likelihood analyses. Nucleic Acids Research 44 (W1): W232–W235. https://doi.org/10.1093/nar/gkw256
  39. Vellinga, E.C. (2001) Agaricaceae. In: Noordeloos, M.E., Kuyper, T.W. & Vellinga, E.C. (Eds.) Flora Agaricina neerlandica 5. Rotterdam: A.A. Balkema Publishers, 220 pp.
  40. White, T.J., Bruns, T., Lee, S. & Taylor, J.W. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis, M.A., Gelfand, D.H., Sninsky, J.J. & White, T.J. (Eds.) PCR protocols: a guide to methods and applications. Academic Press, New York, pp. 315–322. https://doi.org/10.1016/B978-0-12-372180-8.50042-1

How to Cite

Khan, M.B., Sana, Ishaq, M., Kiran, M., Tarafder, E., Fiaz, M. & Wen, T.C. (2026) Entoloma fissuromarginatum (Entolomataceae, Agaricales), a new species from Battagram District, Pakistan. Phytotaxa 739 (2): 134–144. https://doi.org/10.11646/phytotaxa.739.2.2