Abstract
A new species in the genus Amphibambusa (Cainiaceae), isolated from decaying bamboo culms in Guangxi, China, is described and illustrated here under the name Amphibambusa guangxiensis. Phylogenetic analyses of combined ITS and LSU sequence data support its recognition as a novel species based on maximum likelihood and Bayesian inference criteria. Amphibambusa guangxiensis is characterized by larger asci (160–235 μm in length) and larger ascospores (26–34 × 7.3–9.8 μm), which differentiate it from the closely related species A. bambusicola and A. aquatica. Descriptions and illustration of the new species are provided, as well as an updated key for accepted Amphibambusa species.
References
- Bhattacharya, D., Lutzoni, F., Reeb, V., Simon, D., Nason, J. & Fernandez, F. (2000) Widespread occurrence of spliceosomal introns in the rDNA genes of ascomycetes. Molecular Biology and Evolution 17 (12): 1971–1984. https://doi.org/10.1093/oxfordjournals.molbev.a026298
- Darriba, D., Taboada, G.L., Doallo, R. & Posada, D. (2012) jModelTest 2: more models, new heuristics and parallel computing. Nature methods 9 (8): 772. https://doi.org/10.1038/nmeth.2109
- Gardes, M. & Bruns, T.D. (1993) ITS primers with enhanced specificity for basidiomycetes: Application to the identification of mycorrhizae and rusts. Molecular Ecology 2 (2): 113–118. https://doi.org/10.1111/j.1365-294X.1993.tb00005.x
- Hall, T.A. (1999) BioEdit: A user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 41: 95–98.
- Han, L.S., Wijayawardene, N.N., Liu, C., Han, L.H., Promputtha, I., Li, Q., Elgorban, A.M., Al-Rejaie, S., Tanaka, K. & Dai, D.Q. (2024) Paramphibambusa bambusicola gen. et sp. nov., Arecophila xishuangbannaensis and A. zhaotongensis spp. nov. in Cainiaceae from Yunnan, China. MycoKeys 104: 113–132. https://doi.org/10.3897/mycokeys.104.117872
- Hyde, K.D., Norphanphoun, C., Maharachchikumbura, S.S.N., Bhat, D.J., Jones, E.B.G., Bundhun, D., Chen, Y.J., Bao, D.F., Boonmee, S., Calabon, M.S., Chaiwan, N., Chethana, K.W.T., Dai, D.Q., Dayarathne, M.C., Devadatha, B., Dissanayake, A.J., Dissanayake, L.S., Doilom, M., Dong, W., Fan, X.L., Goonasekara, I.D., Hongsanan, S., Huang, S.K., Jayawardena, R.S., Jeewon, R., Karunarathna, A., Konta, S., Kumar, V., Lin, C.G., Liu, J.K., Liu, N.G., Luangsa-ard, J., Lumyong, S., Luo, Z.L., Marasinghe, D.S., McKenzie, E.H.C., Niego, A.G.T., Niranjan, M., Perera, R.H., Phukhamsakda, C., Rathnayaka, A.R., Samarakoon, M.C., Samarakoon, S.M.B.C., Sarma, V.V., Senanayake, I.C., Shang, Q.J., Stadler, M., Tibpromma, S., Wanasinghe, D.N., Wei, D.P., Wijayawardene, N.N., Xiao, Y.P., Yang, J., Zeng, X.Y., Zhang, S.N. & Xiang, M.M. (2020) Refined families of Sordariomycetes. Mycosphere 11 (1): 305–1059. https://doi.org/10.5943/mycosphere/11/1/7
- Jaklitsch, W.M., Gardiennet, A. & Voglmayr, H. (2016) Resolution of morphology-based taxonomic delusions: Acrocordiella, Basiseptospora, Blogiascospora, Clypeosphaeria, Hymenopleella, Lepteutypa, Pseudapiospora, Requienella, Seiridium and Strickeria. Persoonia 37 (1): 82–105. https://doi.org/10.3767/003158516X690475
- Jiang, H., Zhang, S., Phookamsak, R., Promputtha, I., Kakumyan, P. & Xu, J. (2021) Amphibambusa hongheensis sp. nov., a novel bambusicolous ascomycete from Yunnan, China. Phytotaxa 505 (2): 201–212. https://doi.org/10.11646/phytotaxa.505.2.6
- Katoh, K., Rozewicki, J. & Yamada, K.D. (2019) MAFFT online service: Multiple sequence alignment, interactive sequence choice and visualization. Briefings in Bioinformatics 20 (4): 1160–1166. https://doi.org/10.1093/bib/bbx108
- Konta, S., Hyde, K.D., Eungwanichayapant, P.D., Karunarathna, S.C., Samarakoon, M.C., Xu, J., Dauner, L.A.P., Aluthwattha, S.T., Lumyong, S. & Tibpromma, S. (2021) Multigene phylogeny reveals Haploanthostomella elaeidis gen. et sp. nov. and familial replacement of Endocalyx (Xylariales, Sordariomycetes, Ascomycota). Life (Basel, Switzerland) 11 (6): 486. https://doi.org/10.3390/life11060486
- Liu, J.K., Hyde, K.D., E.B.G. Jones, Ariyawansa, H.A., Bhat, J.D., Boonmee, S., Maharachchikumbura, S., McKenzie, E.H.C., Phookamsak, R., Phukhamsakda, C., Shenoy, B.D., Abdel-Wahab, M.A., Buyck, B., Chen, J., Chethana, K.W.T., Singtripop, C., Dai, D., Dai, Y., Daranagama, D.A., Dissanayake, A., Doilom, M., D’souza, M.J., Fan, X.L., Goonasekara, I.D., Hirayama, K., Hongsanan, S., Jayasiri, S.C., Jayawardena, R., Karunarathna, S.C., Li, W.J., Mapook, A., Norphanphoun, C., Pang, K.L., Perera, R.H., Peršoh, D., Pinruan, U., Senanayake, I., Somrithipol, S., Suetrong, S., Tanaka, K., Thambugala, K.M., Tian, Q., Tibpromma, S., Udayanga, D., Wijayawardene, N., Wanasinghe, D., Wisitrassameewong, K., Abdel-Aziz, F.A., Adamčík, S., Bahkali, A.H., Boonyuen, N., Bulgakov, T., Callac, P., Chomnunti, P., Greiner, K., Hashimoto, A., Hofstetter, V., Kang, J.C., Lewis, D., Li, X.H., Liu, X.X., Liu, Z.Y., Matumura, M., Mortimer, P.E., Rambold, G., Randrianjohany, E., Sato, G., Indrasutdhi, V.S., Tian, C.M., Verbeken, A., von Brackel, W., Wang, Y., Wen, T.C., Xu, J.C., Yan, J.Y., Zhao, R.L. & Camporesi, E. (2015) Fungal diversity notes 1–110: taxonomic and phylogenetic contributions to fungal species. Fungal Diversity 72: 1–197. https://doi.org/10.1007/s13225-015-0324-y
- Liu, L.L., Ren, Y.L., Habib, K., Lu, C.T., Wu, Y.P., Long, S.H., Lin, Y., Zhang, X., Kang, Y.Q., Wijayawardene, N.N., Wang, F., Elgorban, A.M., Al-Rejaie, S., Samarakoon, M.C., Shen, X.C. & Li, Q.R. (2025) New taxa of Xylariales from Karst Ecosystems in Southwestern China. Mycosphere 16 (1): 1–78. https://doi.org/10.5943/mycosphere/16/1/1
- Li, Q.R., Zhang, X., Lin, Y., Samarakoon, M.C., Hyde, K.D., Shen, X.C., Liao, W.Q., Karunarathna, A., Long, S.H., Kang, Y.Q. & Kang, J.C. (2022) Morpho-molecular characterisation of Arecophila, with A. australis and A. clypeata sp. nov. and A. miscanthi comb. nov. MycoKeys 88: 123–149. https://doi.org/10.3897/mycokeys.88.79475
- Long, Q.D., Liu, L.L., Zhang, X., Wen, T.C., Kang, J.C., Hyde, K.D., Shen, X.C. & Li, Q.R. (2019) Contributions to species of Xylariales in China-1. Durotheca species. Mycological Progress 18 (3): 495–510. https://doi.org/10.1007/s11557-018-1458-6
- Manawasinghe, I.S., Hyde, K.D., Wanasinghe, D.N., Karunarathna, S.C., Maharachchikumbura, S.S.N., Samarakoon, M.C., Voglmayr, H., Pang, K.L., Chiang, M.W.L., Jones, E.B.G., Saxena, R.K., Kumar, A., Rajeshkumar, K.C., Selbmann, L., Coleine, C., Hu, Y., Ainsworth, A.M., Liimatainen, K., Niskanen, T., Ralaiveloarisoa, A., Arumugam, E., Kezo, K., Kaliyaperumal, M., Gunaseelan, S., Dissanayake, A.J., Khalid, A.N., Gajanayake, A.J., Flakus, A., Armand, A., Aptroot, A., Rodrigues, A., Tsurykau, A., López Villalba, Á., Gomes de Farias, A.R., Sánchez, A., Góes Neto, A., Goto, B.T., de Souza, C.A.F., Chuaseeharonnachai, C., Lin, C.G., Li, C., Denchev, C.M., Mateo, D.G., Tennakoon, D.S., Wei, D.P., Begerow, D., Alves, E., Drechsler Santos, E.R., Sousa, E.S., Medeiros, E.V., Langer, E., Zhang, F., de Souza, F.A., Magurno, F., Barreto, G.G., Moreno, G., Mane, G., Silva, G.A., da Silva, G.A., Xia, G., Shen, H.W., Gui, H., Senanayake, I.C., Luangsa-ard, J.J., Liu, J.W., Liu, J.K., Ma, J., Lin, J.Y., Beserra Jr, J.E.A., Cano Lira, J.F., Gené, J., Harikrishnan, K., Lu, L., dos Santos, L.A., Xu, L., Lacerda, L.T., Gusmão, L.F.P., Cáceres, M.E.S., Câmara, M.P.S., de Barros Barreto, M.B.B., Calabon, M.S., Kukwa, M., Kemler, M., de Melo, M.P., Ghobad-Nejhad, M., Luo, M., Ding, M., Doilom, M., Phonemany, M., Usman, M., Thongklang, N., Boonyuen, N., Ashtekar, N., Kularathnage, N.D., Sruthi, O.P., Kwantong, P., Ansil, P.A., Kooij, P.W., Zhao, Q., Alfenas, R.F., de Oliveira, R.J.V., Singh, R., da Silva, R.M.F., Avchar, R., Morey, R., Sharma, R., Xu, R.J., da Silveira, R.M.B., Xu, R.F., Jayawardena, R.S., Nanu, S., Nuankaew, S., Tibpromma, S., Boonmee, S., Somrithipol, S., Varghese, S., Moreira, S.I., Rajwar, S., He, S.C., Arun Kumar, T.K., Denchev, T.T., Luangharn, T., de Oliveira, T.G.L., Du, T.Y., Wen, T.-C., Du, T., Wu, T., Indrasutdhi, V.S., Doyle, V.P., Baulin, V., Dong, W., Li, W.L., Lu, W.H., Tian, W., Vieira, W.A., von Brackel, W., Yu, X.D., Zhang, X., Liu, X.F., Peng, X.C., Chen, Y., Yang, Y., Gao, Y., Xiong, Y., Shu, Y., Lu, Y.Z., Shen, Y.M., Zhou, Y., Zhang, Y.X., Zhang, W., Luo, Z.L., Madushani, M.A., Cheewangkoon, R., Song, J.G. & Xu, B. (2025) Fungal diversity notes 1818–1918: taxonomic and phylogenetic contributions on genera and species of fungi. Fungal Diversity 130: 1–261. https://doi.org/10.1007/s13225-024-00541-y
- Mapook, A., Hyde, K.D., McKenzie, E.H.C., Jones, E.B.G., Bhat, D.J., Jeewon, R., Stadler, M., Samarakoon, M.C., Malaithong, M., Tanunchai, B., Buscot, F., Wubet, T. & Purahong, W. (2020) Taxonomic and phylogenetic contributions to fungi associated with the invasive weed Chromolaena odorata (Siam weed). https://doi.org/10.1007/s13225-020-00444-8
- Miller, M., Pfeiffer, W.T. & Schwartz, T. (2010) Creating the CIPRES science gateway for inference of large phylogenetic trees. In: Proceedings of the Gateway Computing Environments Workshop (GCE). IEEE, pp. 1–8. https://doi.org/10.1109/GCE.2010.5676129
- Rambaut, A. (2018) FigTree—Tree Figure Drawing Tool Version v. 1.4.4. Institute of Evolutionary Biology, University of Edinburgh.
- Ronquist, F., Teslenko, M., van der Mark, P., Ayres, D.L., Darling, A., Höhna, S., Larget, B., Liu, L., Suchard, M.A. & Huelsenbeck, J.P. (2012) Mrbayes 3.2: Efficient bayesian phylogenetic inference and model choice across a large model space. Systematic Biology 61: 539–542. https://doi.org/10.1093/sysbio/sys029
- Ren, Y.L., Liu, L.L., Yang, Q., Habib, K., Wijayawardene, N., Kang, J.C., Li, Q.R. & Samarakoon, M.C. (2025) Amphibambusa xishuangbannaensis, a new bambusicolous ascomycete (Cainiaceae, Xylariales) from Southwest China. Sydowia. [in press]
- Senanayake, I.C., Maharachchikumbura, S.S., Hyde, K.D., Bhat, J.D., Jones, E.B., McKenzie, E.H., Dai, D., Daranagama, D.A., Dayarathne, M.C., Goonasekara, I.D., Konta, S., Li, W.J., Shang, Q., Stadler, M., Wijayawardene, N.N., Xiao, Y., Norphanphoun, C., Li, Q., Liu, X., Bahkali, A.H., Kang, J., Wang, Y., Wen, T., Wendt, L., Xu, J.C. & Camporesi, E. (2015) Towards unraveling relationships in Xylariomycetidae (Sordariomycetes). Fungal Diversity 73: 73–144. https://doi.org/10.1007/s13225-015-0340-y
- Stamatakis, A. (2014) RAxML version 8: A tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 30 (9): 1312–1313. https://doi.org/10.1093/bioinformatics/btu033
- Nylander, J.A.A. (2004) MrModeltest v. 2.2. Program distributed by the author. Uppsala University, Department of systematic zoology, Uppsala, Sweden.
- Voglmayr, H., Friebes, G., Gardiennet, A. & Jaklitsch, W.M. (2018) Barrmaelia and Entosordaria in Barrmaeliaceae (fam. nov., Xylariales) and critical notes on Anthostomella -like genera based on multigene phylogenies. Mycological progress 17 (1): 155–177. https://doi.org/10.1007/s11557-017-1329-6
- Vu, D., Groenewald, M., de Vries, M., Gehrmann, T., Stielow, B., Eberhardt, U., Al-Hatmi, A., Groenewald, J.Z., Cardinali, G., Houbraken, J., Boekhout, T., Crous, P.W., Robert, V. & Verkley, G.J.M. (2019) Large-scale generation and analysis of filamentous fungal DNA barcodes boosts coverage for kingdom fungi and reveals thresholds for fungal species and higher taxon delimitation. Studies in Mycology 92 (1): 135–154. https://doi.org/10.1016/j.simyco.2018.05.001
- Vilgalys, R. & Hester, M. (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. Journal of Bacteriology 172: 4239–4246. https://doi.org/10.1128/jb.172.8.4238-4246.1990
- White, T.J. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis, M.A., Gelfand, D.H., Sninsky, J.J. & White, T.J. (Eds.) PCR Protocols: A guide to methods and applications. Academic Press, pp. 315–322. https://doi.org/10.1016/B978-0-12-372180-8.50042-1
- Xie, X.L., Liu, L.L., Shen, X.C., Kang, Y.Q. & Li, Q.R. (2020) Contributions to species of Xylariales in China-3. Collodiscula tubulosa (Xylariaceae). Phytotaxa 428: 122–130. https://doi.org/10.11646/phytotaxa.428.2.6
- Zhang, J.F., Liu, J.K., Hyde, K.D., Chen, Y.Y., Ran, H.Y. & Liu, Z.Y. (2023) Ascomycetes from karst landscapes of Guizhou Province, China. Fungal Diversity 122 (1): 1–160. https://doi.org/10.1007/s13225-023-00524-5
- Zhou, X., Habib, K., Zeng, W., Ren, Y., Shen, X., Kang, J. & Li, Q. (2024) Addition of three new species of Xylariomycetidae fungi on bamboo from Southern China. MycoKeys 109: 109–129. https://doi.org/10.3897/mycokeys.109.128020
