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Issue: Vol. 704 No. 3: 10 Jun. 2025
Type: Article
Published: 2025-06-10
DOI: 10.11646/phytotaxa.704.3.5
Page range: 271-282
Abstract views: 121
PDF downloaded: 7

Ophiocordyceps anocheti sp. nov. a new ant-parasitizing species from China

School of Food and Pharmaceutical Engineering, Guizhou Institute of Technology, Guiyang 550025, P.R. China; Guizhou Key Laboratory of Agricultural Microbiology, Guizhou Academy of Agricultural Sciences, Guiyang 550009, China
School of Food and Pharmaceutical Engineering, Guizhou Institute of Technology, Guiyang 550025, P.R. China; Guizhou Key Laboratory of Agricultural Microbiology, Guizhou Academy of Agricultural Sciences, Guiyang 550009, China; Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand; School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand
novel taxon Hypocreales morphological phylogeny Fungi

Abstract

Ophiocordyceps is the largest genus in Ophiocordycipitaceae, known for its diverse host range and ecological adaptability, particularly in tropical and subtropical regions. Southwestern China is a hotspot for its species diversity. Ophiocordyceps fungi typically parasitize insects through specialized adaptations in their life cycle. In this study, a new species, Ophiocordyceps anocheti, a parasite of red ants (Anochetus sp., Ponerinae) is described. It is distinguished from other Ophiocordyceps species in having slender synnemata lacking a swollen head, stout phialides, and hyaline, smooth, one-celled conidia. Phylogenetic analysis based on six loci (SSU, LSU, ITS, tef-1α, rpb1, and rpb2) confirms Ophiocordyceps anocheti as a novel species within the genus.

References

  1. Araújo, J.P.M. & Hughes, D.P. (2017) The fungal spore: Myrmecophilous Ophiocordyceps as a case study. In: Dighton, J. & White, J.M. (Eds.) The fungal community: its organization and role in the ecosystem, 4th edn. CRC Press, USA, pp. 359–367. https://doi.org/10.1201/9781315119496-25
  2. Araújo, J.P.M., Evans, H.C., Geiser, D.M., Mackay, W.P. & Hughes, D.P. (2015) Unravelling the diversity behind the Ophiocordyceps unilateralis (Ophiocordycipitaceae) complex: three new species of zombie-ant fungi from the Brazilian Amazon. Phytotaxa 220 (3): 224–238. https://doi.org/10.11646/phytotaxa.220.3.2
  3. Araújo, J.P.M., Evans, H.C., Kepler, R. & Hughes, D.P. (2018) Zombie-ant fungi across continents: 15 new species and new combinations within Ophiocordyceps. I. Myrmecophilous hirsutelloid species. Studies in Mycology 90: 119–160. https://doi.org/10.1016/j.simyco.2017.12.002
  4. Araújo, J.P.M., Moriguchi, M.G., Uchiyama, S., Kinjo, N. & Matsuura, Y. (2021) Ophiocordyceps salganeicola, a parasite of social cockroaches in Japan and insights into the evolution of other closely related Blattodea-associated lineages. IMA Fungus 12 (1): 1–17. https://doi.org/10.1186/s43008-020-00053-9
  5. Ban, S., Sakane, T. & Nakagiri, A. (2015) Three new species of Ophiocordyceps and overview of anamorph types in the genus and the family Ophiocordyceptaceae. Mycological Progress 14 (1): 1–12. https://doi.org/10.1007/s11557-014-1017-8
  6. Capella-Gutiérrez, S., Silla-Martínez, J.M. & Gabaldón, T. (2009) trimAl: a tool for automated alignment trimming in large-scale phylogenetic analyses. Bioinformatics 25 (15): 1972–1973. https://doi.org/10.1093/bioinformatics/btp348
  7. Castlebury, L.A., Rossman, A.Y., Sung, G.H., Hyten, A.S. & Spatafora, J.W. (2004) Multigene phylogeny reveals new lineage for Stachybotrys chartarum, the indoor air fungus. Mycological Research 108 (8): 864–872. https://doi.org/10.1017/S0953756204000607
  8. Chao, S.C., Hsieh, I.L., Lee, L.W., Cheng, K.T. & Su, C.H. (2004) Culture of Cordyceps myrmecophila Cessati. Fungal Science 19 (1, 2): 53–59.
  9. De Bekker, C., Quevillon, L., Smith, P., Fleming, K., Ghosh, D., Patterson, A. & Hughes, D. (2014) Species-specific ant brain manipulation by a specialized fungal parasite. BMC Evolutionary Biology 14. https://doi.org/10.1186/PREACCEPT-8313271611363357
  10. Evans, H.C. & Samson, R.A. (1982) Cordyceps species and their anamorphs pathogenic on ants (Formicidae) in tropical forest ecosystem. I. The Cephalotes (Myrmicinae) complex. Transactions of the British Mycological Society 79: 431–453. https://doi.org/10.1016/S0007-1536(82)80037-5
  11. Evans, H.C. & Samson, R.A. (1984) Cordyceps species and their anamorphs pathogenic on ants (Formicidae) in tropical forest ecosystems II. The Camponotus (Formicinae) complex. Transactions of the British Mycological Society 82 (1): 127–150. https://doi.org/10.1016/S0007-1536(84)80219-3
  12. Evans, H.C., Elliot, S.L. & Hughes, D.P. (2011) Hidden diversity behind the zombie-ant fungus Ophiocordyceps unilateralis: four new species described from carpenter ants in Minas Gerais, Brazil. PLoS One 6 (3): e17024. https://doi.org/10.1371/journal.pone.0017024
  13. Goecks, J., Nekrutenko, A. & Taylor, J. (2010) Galaxy: a comprehensive approach for supporting accessible, reproducible, and transparent computational research in the life sciences. Genome Biology 11: 1–13. https://doi.org/10.1186/gb-2010-11-8-r86
  14. Hall, T., Biosciences, I. & Carlsbad, C. (2011) BioEdit: an important software for molecular biology. GERF Bulletin of Biosciences 2 (1): 60–61.
  15. Hoang, D.T., Chernomor, O., von Haeseler, A., Minh, B.Q. & Vinh, L.S. (2018) UFBoot2: improving the Ultrafast Bootstrap Approximation. Molecular Biology and Evolution 35 (2): 518–522. https://doi.org/10.1093/molbev/msx281
  16. Hywel-Jones, N. (1996) Cordyceps myrmecophila-like fungi infecting ants in the leaf litter of tropical forest in Thailand. Mycological Research 100 (5): 613–619. https://doi.org/10.1016/S0953-7562(96)80017-7
  17. Jayasiri, S.C., Hyde, K.D., Ariyawansa, Bhat, J., Buyck, B., Cai, L., Dai, Y.C., Abd-Elsalam, K.A., Ertz, D., Hidayat, I., Jeewon, R., Jones, E.B.G., Bahkali, A.H., Karunarathna, S.C., Liu, J.K., Luangsa-ard, J.J., Lumbsch, H.T., Maharachchikumbura, S.S.N., McKenzie, E.H.C., Moncalvo, J.M., Ghobad-Nejhad, M., Nilsson, H., Pang, K.L., Pereira, O.L., Phillips, A.J.L., Raspe, O. & Rollins, A.W., Romero, A.I., Etayo, J., Selcuk, F., Stephenson, S.L., Suetrong, S., Taylor, J.E., Tsui, C.K.M., Vizzini, A., Abdel-Wahab, M.A., Wen, T.C., Boonmee, S., Dai, D.Q., Daranagama, D.A., Dissanayake, A.J., Ekanayaka, A.H., Fryar, S.C., Hongsanan, S., Jayawardena, R.S., Li, W.J., Perera, R.H., Phookamsak, R., De Silva, N.I., Thambugala, K.M., Tian, Q., Wijayawardene, N.N., Zhao, R.L. & Zhao, Q., Kang, J.C. & Promputtha, I. (2015) The Faces of Fungi database: fungal names linked with morphology, phylogeny and human impacts. Fungal Diversity 74 (1): 3–18. https://doi.org/10.1007/s13225-015-0351-8
  18. Kalyaanamoorthy, S., Minh, B.Q., Wong, T.K.F., von Haeseler, A. & Jermiin, L.S. (2017) ModelFinder: fast model selection for accurate phylogenetic estimates. Nature Methods 14 (6): 587–589. https://doi.org/10.1038/nmeth.4285
  19. Katoh, K. & Standley, D.M. (2013) MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Molecular Biology and Evolution 30 (4): 772–780. https://doi.org/10.1093/molbev/mst010
  20. Khonsanit, A., Luangsa-ard, J.J., Thanakitpipattana, D., Kobmoo, N. & Piasai, O. (2019) Cryptic species within Ophiocordyceps myrmecophila complex on formicine ants from Thailand. Mycological Progress 18: 147–161. https://doi.org/10.1007/s11557-018-1412-7
  21. Kobayasi, Y. (1941) The genus Cordyceps and its allies. Science Reports of the Tokyo Bunrika Daigaku 5: 53–260.
  22. Kobayasi, Y. & Shimizu, D. (1981) The genus Cordyceps and its allies from Taiwan (Formosa). Bulletin of the National Museum of Nature and Science 7: 113–122.
  23. Kobmoo, N., Mongkolsamrit, S., Tasanathai, K., Thanakitpipattana, D. & Luangsa-ard, J.J. (2012) Molecular phylogenies reveal host–specific divergence of Ophiocordyceps unilateralis sensu lato following its host ants. Molecular Ecology 21 (12): 3022–3031. https://doi.org/10.1111/j.1365-294X.2012.05574.x
  24. Kobmoo, N., Mongkolsamrit, S., Wutikhun, T., Tasanathai, K., Khonsanit, A., Thanakitpipattana, D. & Luangsa-ard, J.J. (2015) New species of Ophiocordyceps unilateralis, a ubiquitous pathogen of ants from Thailand. Fungal Biology 119 (1): 44–52. https://doi.org/10.1016/j.funbio.2014.10.008
  25. Luangsa-ard, J.J., Ridkaew, R., Tasanathai, K., Thanakitpipattana, D. & Hywel-Jones, N. (2011) Ophiocordyceps halabalaensis: a new species of Ophiocordyceps pathogenic to Camponotus gigas in Hala Bala Wildlife Sanctuary, Southern Thailand. Fungal Biology 115 (7): 608–614. https://doi.org/10.1016/j.funbio.2011.03.002
  26. Luangsa-ard, J.J., Tasanathai, K., Thanakitpipattana, D., Khonsanit, A. & Stadler, M. (2018) Novel and interesting Ophiocordyceps spp. (Ophiocordycipitaceae, Hypocreales) with superficial perithecia from Thailand. Studies in Mycology 89: 125–142. https://doi.org/10.1016/j.simyco.2018.02.001
  27. Maharachchikumbura, S.S.N., Hyde, K.D., Jones, E.B.G., McKenzie, E.H.C., Huang, S.K., Abdel-Wahab, M.A., Daranagama, D.A., Dayarathne, M., D’souza, M.J., Goonasekara, I.D., Hongsanan, S., Jayawardena, R.S., Kirk, P.M., Konta, S., Liu, J.K., Liu, Z.Y., Norphanphoun, C., Pang, K.L., Perera, R.H., Senanayake, I.C., Shang, Q.J., Shenoy, B.D., Xiao, Y.P., Bahkali, A.H., Kang, J.C. & Xu, J.C. (2015) Towards a natural classification and backbone tree for Sordariomycetes. Fungal Diversity 72 (1): 199–301. https://doi.org/10.1007/s13225-015-0331-z
  28. Maharachchikumbura, S.S.N., Hyde, K.D., Jones, E.B.G., McKenzie, E.H.C., Bhat, D.J., Dayarathne, M.C., Huang, S.K., Norphanphoun, C., Senanayake, I.C., Perera, R.H., Shang, Q.J., Xiao, Y., D’souza, M.J., Hongsanan, S., Jayawardena, R.S., Daranagama, D.A., Konta, S., Goonasekara, I.D., Zhuang, W.Y., Jeewon, R., Phillips, A.J.L., Abdel-Wahab, M.A., Al-Sadi, A.M., Bahkali, A.H., Boonmee, S., Boonyuen, N., Cheewangkoon, R., Dissanayake, A.J., Kang, J., Li, Q.R., Liu, J.K., Liu, X.Z., Liu, Z.Y., Luangsa-ard, J.J., Pang, K.L., Phookamsak, R., Promputtha, I., Suetrong, S., Stadler, M., Wen, T.C. & Wijayawardene, N.N. (2016) Families of Sordariomycetes. Fungal Diversity 79: 1–317. https://doi.org/10.1007/s13225-016-0369-6
  29. Mains, E.B. (1940) Species of Cordyceps. Mycologia 32 (3): 310–320. https://doi.org/10.2307/3754313
  30. Nguyen, L.T., Schmidt, H.A., von Haeseler, A. & Minh, B.Q. (2015) IQ-TREE: a fast and effective stochastic algorithm for estimating maximum-likelihood phylogenies. Molecular Biology and Evolution 32 (1): 268–274. https://doi.org/10.1093/molbev/msu300
  31. Petch, T. (1931) Notes on entomogenous fungi. Transactions of the British Mycological Society 16: 55–75. https://doi.org/10.1016/S0007-1536(31)80006-3
  32. Qian, L.S., Chen, J.H., Deng, T. & Sun, H. (2020) Plant diversity in Yunnan: current status and future directions. Plant Diversity 42: 281–291. https://doi.org/10.1016/j.pld.2020.07.006
  33. Quandt, C.A., Kepler, R.M., Gams, W., Araújo, J.P.M., Ban, S., Evans, H.C., Hughes, D., Humber, R., Hywel-Jones, N., Li, Z.Z., Luangsa-ard, J.J., Rehner, S.A., Sanjuan, T., Sato, H., Shrestha, B., Sung, G.H., Yao, Y.J., Zare, R. & Spatafora, J.W. (2014) Phylogenetic-based nomenclatural proposals for Ophiocordycipitaceae (Hypocreales) with new combinations in Tolypocladium. IMA Fungus 5 (1): 121–134. https://doi.org/10.5598/imafungus.2014.05.01.12
  34. Rabenhorst, L. (1846) Klotzschii Herbarium vivum mycologicum sistens Fungorum per totam Germanium crescentium collectionem perfectam. Centuria unidecima Dresdae. Botanische Zeitung (Berlin) 4: 876–880. https://doi.org/10.5962/bhl.title.140325
  35. Rambaut, A. (2016) FigTree version 1.4.3. Available from: https://github.com/rambaut/figtree/releases/tag/v1.4.3 (accessed 9 June 2025)
  36. Ronquist, F., Teslenko, M., van der Mark, P., Ayres, D.L., Darling, A., Höhna, S., Larget, B., Liu, L. & Suchard, M.A. & Huelsenbeck, J.P. (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology 61 (3): 539–542. https://doi.org/10.1093/sysbio/sys029
  37. Saccardo, P.A. (1883) Sylloge pyrenomycetum. Vol II. Sylloge Fungorum 2: 1–813.
  38. Sanjuan, T.I., Franco-Molano, A.E., Kepler, R.M., Spatafora, J.W., Tabima, J., Vasco-Palacios, A.M. & Restrepo, S. (2015) Five new species of entomopathogenic fungi from the Amazon and evolution of neotropical Ophiocordyceps. Fungal Biology 119 (10): 901–916. https://doi.org/10.1016/j.funbio.2015.06.010
  39. Shrestha, B., Tanaka, E., Hyun, M.W., Han, J.G., Kim, C.S., Jo, J.W., Han, S.K., Oh, J. & Sung, G.H. (2017) Mycosphere essay 19. Cordyceps species parasitizing hymenopteran and hemipteran insects. Mycosphere 8 (9): 1424–1442. https://doi.org/10.5943/mycosphere/8/9/8
  40. Simmons, D.R., Lund, J., Levitsky, T. & Groden, E. (2015) Ophiocordyceps myrmicarum, a new species infecting invasive Myrmica rubra in Maine. Journal of Invertebrate Pathology 125: 23–30. https://doi.org/10.1016/j.jip.2014.12.010
  41. Sung, G.H., Hywel-Jones, N.L., Sung, J.M., Luangsa-ard, J.J., Shrestha, B. & Spatafora, J.W. (2007) Phylogenetic classification of Cordyceps and the clavicipitaceous fungi. Studies in Mycology 57: 5–59. https://doi.org/10.3114/sim.2007.57.01
  42. Tang, D., Huang, O., Zou, W., Wang, Y., Wang, Y., Dong, Q., Sun, T., Yang, G. & Yu, H. (2023) Six new species of zombie-ant fungi from Yunnan in China. IMA Fungus 14 (1): 9. https://doi.org/10.1186/s43008-023-00114-9
  43. Tang, D.X., Zhu, J.Y., Luo, L.Y., Hou, D.H., Wang, Z.Q., Yang, S.D. & Yu, H. (2022) Ophiocordyceps ovatospora sp. nov. (Ophiocordycipitaceae, Hypocreales), pathogenic on termites from China. Phytotaxa 574 (1): 105–117. https://doi.org/10.11646/phytotaxa.574.1.8
  44. Tasanathai, K., Khonsanit, A., Noisripoom, W., Kobmoo, N. & Luangsa-ard, J.J. (2022) Hidden species behind Ophiocordyceps (Ophiocordycipitaceae, Hypocreales) on termites: four new species from Thailand. Mycological Progress 21 (10): 86–102. https://doi.org/10.1007/s11557-022-01837-6
  45. Tasanathai, K., Noisripoom, W., Chaitika, T., Khonsanit, A., Hasin, S. & Luangsa-ard, J.J. (2019) Phylogenetic and morphological classification of Ophiocordyceps species on termites from Thailand. MycoKeys 56: 101–129. https://doi.org/10.3897/mycokeys.56.37636
  46. Vilgalys, R. & Hester, M. (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. Journal of Bacteriology 172 (8): 4238–4246. https://doi.org/10.1128/jb.172.8.4238-4246.1990
  47. Wijayawardene, N.N., Dissanayake, L.S., Li, Q.R., Dai, D.Q., Xiao, Y.P., Wen, T.C., Karunarathna, S.C., Wu, H.X., Zhang, H., Tibpromma, S., Kang, J.C., Wang, Y., Shen, X.C., Tang, L.Z., Deng, C.Y., Liu, Y.X. & Kang, Y.Q. (2021) Yunnan–Guizhou Plateau: a mycological hotspot. Phytotaxa 523 (1): 1–31. https://doi.org/10.11646/phytotaxa.523.1.1
  48. Xiao, Y.P., Hongsanan, S., Hyde, K.D., Brooks, S., Xie, N., Long, F.Y. & Wen, T.C. (2019) Two new entomopathogenic species of Ophiocordyceps in Thailand. MycoKeys 47: 53–74. https://doi.org/10.3897/mycokeys.47.29898
  49. Xiao, Y.P., Wen, T.C., Hongsanan, S., Sun, J.Z. & Hyde, K.D. (2017) Introducing Ophiocordyceps thanathonensis, a new species of entomogenous fungi on ants, and a reference specimen for O. pseudolloydii. Phytotaxa 328 (2): 115–126. https://doi.org/10.11646/phytotaxa.328.2.2
  50. Yang, Y., Jayawardena, R.S., Lu, Y.Z., Xie, S.Q., Tian, X.G., Wang, J.P., Zhou, S.X. & Xiao, Y.P. (2024) Four new Ophiocordyceps species in China. Mycosystema 43 (3): 230–256. https://doi.org/10.13346/j.mycosystema.230256
  51. Yang, Y., Xiao, Y.P., Yu, G.J., Wen, T.C., Deng, C.Y., Meng, J. & Lu, Z.H. (2021) Ophiocordyceps aphrophoridarum sp. nov., a new entomopathogenic species from Guizhou, China. Biodiversity Data Journal 9: e66115. https://doi.org/10.3897/BDJ.9.e66115
  52. Yang, Y., Xiao, Y.P., Jayawardena, S.R., Hyde, K.D., Nilthong, S., Mapook, A., Lu, Y.Z., Xie, S.Q., Al-Otibi, F., Wang, X., Luo, K., Luo, L.P. (2025) Three new species of Ophiocordyceps (Hypocreales, Ophiocordycipitaceae) and a new host record for Hirsutella vermicola from China. MycoKeys 117: 289–313. https://doi.org/10.3897/mycokeys.117.144875
  53. Zeng, X.Y., Tan, T.J., Tian, F.H., Wang, Y. & Wen, T.C. (2023) OFPT: a one-stop software for fungal phylogeny. Mycosphere 14 (1): 1730–1741. https://doi.org/10.5943/mycosphere/14/1/20
  54. Zou, W., Tang, D., Xu, Z., Huang, O., Wang, Y., Tran, N.L. & Yu, H. (2022) Multigene phylogeny and morphology reveal Ophiocordyceps hydrangea sp. nov. and Ophiocordyceps bidoupensis sp. nov. (Ophiocordycipitaceae). MycoKeys 92: 109–130. https://doi.org/10.3897/mycokeys.92.86160

How to Cite

Xie, S.-Q., Yang, Y., Xiao, Y.-P., Lu, Y.-Z. & Nalumpang, S. (2025) Ophiocordyceps anocheti sp. nov. a new ant-parasitizing species from China. Phytotaxa 704 (3): 271–282. https://doi.org/10.11646/phytotaxa.704.3.5