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Issue: Vol. 696 No. 3: 3 Apr. 2025
Type: Article
Published: 2025-04-03
DOI: 10.11646/phytotaxa.696.3.1
Page range: 205-216
Abstract views: 66
PDF downloaded: 52

Molecular phylogeny and morphology reveal a new species of Radulomyces (Radulomycetaceae, Agaricales) from Southwestern China

Yunnan Tongbiguan Provincial Nature Reserve, Mangshi 679319, P.R. China
Yunnan Tongbiguan Provincial Nature Reserve, Mangshi 679319, P.R. China
Yunnan Tongbiguan Provincial Nature Reserve, Mangshi 679319, P.R. China
Yunnan Tongbiguan Provincial Nature Reserve, Mangshi 679319, P.R. China
Yunnan Tongbiguan Provincial Nature Reserve, Mangshi 679319, P.R. China
College of Forestry, Southwest Forestry University, Kunming 650224, P.R. China
College of Forestry, Southwest Forestry University, Kunming 650224, P.R. China
1 new species Biodiversity Molecular systematics Taxonomy Wood-inhabiting fungi Yunnan Province Fungi

Abstract

Radulomyces tongbiguanensis sp. nov., discovered in Yunnan Province, southwestern China, is described here as a new species based on its morphology and phylogenetic analysis. Radulomyces tongbiguanensis is characterized by its membranaceous basidiomata, which have a smooth hymenial surface and a monomitic hyphal system bearing clamp connections on the generative hyphae, and globose basidiospores measuring 9.5–11.5 × 9–11 µm. Phylogenetic analyses of the new species were conducted based on internal transcribed spacer (ITS) and large subunit (LSU) ribosomal DNA (rDNA) sequences. The phylogenetic analysis indicated that the new species belongs to the genus Radulomyces, and is retrieved grouped with the species R. zixishanensis. However, morphologically R. zixishanensis differs from R. tongbiguanensis by cream to slightly brown basidiomata, narrower basidia (28–43 × 6.5–8.5 µm vs. 28.5–40 × 10–12.5 µm), and ellipsoid, narrower basidiospores (7–8.8 × 5.5–6.8 µm vs. 9.5–11.5 × 9–11 µm). A full description, illustrations, and phylogenetic analysis results of the new species are provided.

References

  1. Bernicchia, A. & Gorjón, S.P. (2010) Fungi Europaei 12: Corticiaceae s.l. Edizioni Candusso, Lomazzo, 1007 pp.
  2. Christiansen, M.P. (1960) Danish resupinate fungi. Part II. Homobasidiomycetes. Dansk botanisk Arkiv 19: 57–388.
  3. Cui, B.K., Li, H.J., Ji, X., Zhou, J.L., Song, J., Si, J., Yang, Z.L. & Dai, Y.C. (2019) Species diversity, taxonomy and phylogeny of Polyporaceae (Basidiomycota) in China. Fungal Diversity 97: 137–392. https://doi.org/10.1007/s13225-019-00427-4
  4. Darriba, D., Taboada, G.L., Doallo, R. & Posada, D. (2012) jModelTest 2: more models, new heuristics and parallel computing. Nature Methods 9: 772. https://doi.org/10.1038/nmeth.2109
  5. Dong, J., Deng, Q., Chen, M., Chen, D., Zhou, C. & Zhao, C. (2025) Molecular phylogeny and morphology reveal four new wood-inhabiting fungi of Asterostroma and Radulomyces (Basidiomycota) from Southwestern China. MycoKeys 112: 35–58. https://doi.org/10.3897/mycokeys.112.137098
  6. Dong, J.H., Li, Q., Yuan, Q., Luo, Y.X., Zhang, X.C., Dai, Y.F., Zhou, Q., Liu, X.F., Deng, Y.L., Zhou, H.M., Muhammad, A. & Zhao, C.L. (2024) Species diversity, taxonomy, molecular systematics and divergence time of wood-inhabiting fungi in Yunnan-Guizhou Plateau, Asia. Mycosphere 15 (1): 1110–1293. https://doi.org/10.5943/mycosphere/15/1/10
  7. Felsenstein, J. (1985) Confidence intervals on phylogenetics: an approach using bootstrap. Evolution 39: 783–791. https://doi.org/10.1111/j.1558-5646.1985.tb00420.x
  8. Ginns, J. & Millman, L. (2011) Mysterious Asian beauty conquers Massachusetts. Fungi, The Nippon Fungological Soc (Tokyo) 4: 61–63.
  9. Hu, Y., Karunarathna, S.C., Li, H., Galappaththi, M.C., Zhao, C.L., Kakumyan, P. & Mortimer, P.E. (2022) The impact of drying temperature on basidiospore size. Diversity 14 (4): 239. https://doi.org/10.3390/d14040239
  10. Hyde, K.D., Baldrian, P., Chen, Y., Chethana, K.W.T., Hoog, S.D., Doilom, M., de Farias, A.R.G., Gonçalves, M.F.M., Gonkhom, D., Gui, H., Hilário, S., Hu, Y.W., Jayawardena, R.S., Khyaju, S., Kirk, P.M., Kohout, P., Luangharn, T., Maharachchikumbura, S.S.N., Manawasinghe, I.S., Mortimer, P.E., Niego, A.G.T., Phonemany, M., Sandargo, B., Senanayake, I.C., Stadler, M., Surup, F., Thongklang, N., Wanasinghe, D.N., Bahkali, A.H. & Walker, A. (2024a) Current trends, limitations and future research in the fungi? Fungal Diversity 125: 1–71. https://doi.org/10.1007/s13225-023-00532-5
  11. Hyde, K.D., Saleh, A., Aumentado, H.D.R., Boekhout, T., Bera, I., Khyaju, S., Bhunjun, C.S., Chethana, K.W.T., Phukhamsakda, C., Doilom, M., Thiyagaraja, V., Mortimer, P.E., Maharachchikumbura, S.S.N., Hongsanan, S., Jayawardena, R.S., Dong, W., Jeewon, R., Al-Otibi, F., Wijesinghe, S.N. & Wanasinghe, D.N. (2024b) Fungal numbers: global needs for a realistic assessment. Fungal Diversity 128: 191–225. https://doi.org/10.1007/s13225-024-00545-8
  12. Katoh, K., Rozewicki, J. & Yamada, K.D. (2019) MAFFT online service: multiple sequence alignment, interactive sequence choice and visualization. Briefings in Bioinformatics 20: 1160–1166. https://doi.org/10.1093/bib/bbx108
  13. Larsson, A. (2014) AliView: a fast and lightweight alignment viewer and editor for large data sets. Bioinformatics 30: 3276–3278. https://doi.org/10.1093/bioinformatics/btu531
  14. Larsson, K.H. (2007) Re-thinking the classification of corticioid fungi. Mycological Research 111: 1040–1063. https://doi.org/10.1016/j.mycres.2007.08.001
  15. Larsson, K.H., Larsson, E. & Kõljalg, U. (2004) High phylogenetic diversity among corticioid Homobasidiomycetes. Mycological Research 108: 983–1002. https://doi.org/10.1017/s0953756204000851
  16. Leal-Dutra, C.A., Griffith, G.W., Neves, M.A., McLaughlin, D.J., McLaughlin, E.G., Clasen, L.A. & Dentinger, B.T.M. (2020) Reclassification of Pterulaceae Corner (Basidiomycota: Agaricales) introducing the ant-associated genus Myrmecopterula gen. nov., Phaeopterula Henn. and the corticioid Radulomycetaceae fam. nov. IMA Fungus 11: 1–24. https://doi.org/10.1186/s43008-019-0022-6
  17. Liu, S., Chen, Y.Y., Sun, Y.F., He, X.L., Song, C.G., Si, J., Liu, D.M., Gates, G. & Cui, B.K. (2022) Systematic classification and phylogenetic relationships of the brown-rot fungi within the Polyporales. Fungal Diversity 118: 1–94. https://doi.org/10.1007/s13225-022-00511-2
  18. Liu, S., Shen, L.L., Xu, T.M., Song, C.G., Gao, N., Wu, D.M., Sun, Y.F. & Cui, B.K. (2023) Global diversity, molecular phylogeny and divergence times of the brown‑rot fungi within the Polyporales. Mycosphere 14: 1564–1664. https://doi.org/10.5943/mycosphere/14/1/18
  19. Liu, S.L., Liu, D.M. & Zhou, L.W. (2024) The future is now: how to conserve fungi. Biological Diversity 1 (1): 6–8. https://doi.org/10.1002/bod2.12003
  20. Mao, W.L., Wu, Y.D., Liu, H.G., Yuan, Y. & Dai, Y.C. (2023) A contribution to Porogramme (Polyporaceae, Agaricomycetes) and related genera. IMA fungus 14: 5. https://doi.org/10.1186/s43008-023-00110-z
  21. Miller, M.A., Pfeiffer, W. & Schwartz, T. (2012) The CIPRES science gateway. In: Innis, M.A., Gelfand, D.H., Sninsky, J.J. & White, T.J. (Eds.) PCR protocols: A guide to methods and applications. Academic Press, San Diego, pp. 1–39. https://doi.org/10.1145/2335755.2335836
  22. Ming, B., Zhou, L.W., Tong, Y.J. & Yin, F. (2023) Risk assessment and warning system for strategic biological resources in China. The Innovation Life 1: 100004. https://doi.org/10.59717/j.xinn‐life.2023.100004
  23. Olariaga, I., Huhtinen, S., Læssøe, T., Petersen, J.H. & Hansen, K. (2020) Phylogenetic origins and family classification of typhuloid fungi, with emphasis on Ceratellopsis, Macrotyphula and Typhula (Basidiomycota). Studies in Mycology 96: 155–184. https://doi.org/10.1016/j.simyco.2020.05.003
  24. Petersen, J.H. (1996) Farvekort. In: The Danish Mycological Society’s Colour-Chart, Foreningen til Svampekundskabens Fremme: Greve, Germany, pp. 1–6.
  25. Rathnayaka, A.R., Tennakoon, D.S., Jones, G.E., Wanasinghe, D.N., Bhat, D.J., Priyashantha, A.H., Stephenson, S.L. & Tibpromma, S. & Karunarathna, S.C. (2025) Significance of precise documentation of hosts and geospatial data of fungal collections, with an emphasis on plant-associated fungi. New Zealand Journal of Botany 63 (2–3): 462–489. https://doi.org/10.1080/0028825X.2024.2381734
  26. Rehner, S.A. & Samuels, G.J. (1994) Taxonomy and phylogeny of Gliocladium analysed fromnuclear large subunit ribosomal DNA sequences. Mycological Research 98 (6): 625–634. https://doi.org/10.1016/S0953-7562(09)80409-7
  27. Ronquist, F., Teslenko, M., van der Mark, P., Ayres, D.L., Darling, A., Hohna, S., Larget, B., Liu, L. & Suchard, M.A. & Huelsenbeck, J.P. (2012) Mrbayes 3.2: efficient bayesian phylogenetic inference and model choice across a large model space. Molecular Systems Biology 61: 539–542. https://doi.org/10.1093/sysbio/sys029
  28. Song, J., Karunarathna, S.C., Senanayake, I.C. & Yu, F.Q. (2024) Thyridium livistonae sp. nov. from Yunnan Province, China, with two new combinations. New Zealand Journal of Botany 62: 1–15. https://doi.org/10.1080/0028825X.2024.2384470
  29. Varga, T., Krizsán, K., Földi, C., Dima, B., Sánchez-García, M., Sánchez-Ramírez, S., Szöllősi, G.J., Szarkándi, J.G., Papp, V., Albert, L., Andreopoulos, W., Angelini, C., Antonín, V., Barry, K.W., Bougher, N.L., Buchanan, P., Buyck, B., Bense, V., Catcheside, P., Chovatia, M., Cooper, J., Dämon, W., Desjardin, D., Finy, P., Geml, J., Haridas, S., Hughes, K., Justo, A., Karasiński, D., Kautmanova, I., Kiss, B., Kocsubé, S., Kotiranta, H., LaButti, K.M., Lechner, B.E., Liimatainen, K., Lipzen, A., Lukács, Z., Mihaltcheva, S., Morgado, L.N., Niskanen, T., Noordeloos, M.E., Ohm, R.A., Ortiz-Santana, B., Ovrebo, C., Rácz, N., Riley, R., Savchenko, A., Shiryaev, A., Soop, K., Spirin, V., Szebenyi, C., Tomšovský, M., Tulloss, R.E., Uehling, J., Grigoriev, I.V., Vágvölgyi, C., Papp, T., Martin, F.M. & Miettinen, O. & Hibbett, D.S. & Nagy, L.G. (2019) Mega-phylogeny resolves global patterns of mushroom evolution. Nature Ecology & Evolution 3 (4): 668–678. https://doi.org/10.1038/s41559-019-0834-1
  30. Vilgalys, R. & Hester, M. (1990) Rapid genetic identification and mapping of enzymaticallyamplified ribosomal DNA from several Cryptococcus species. Journal of Bacteriology 172 (8): 4238–4246. https://doi.org/10.1128/jb.172.8.4238-4246.1990
  31. Wang, C.G., Zhao, H., Liu, H.G., Zeng, G.Y., Yuan, Y. & Dai, Y.C. (2023) A multi‑gene phylogeny clarifies species diversity, taxonomy, and divergence times of Ceriporia and other related genera in Irpicaceae (Polyporales, Basidiomycota). Mycosphere 14 (1): 1665–1729. https://doi.org/10.5943/mycosphere
  32. Wang, C.G., Dai, Y.C., Kout, J., Gates, G.M., Liu, H.G., Yuan, Y. & Vlasák, J. (2024) Multi-gene phylogeny and taxonomy of Physisporinus (Polyporales, Basidiomycota). Mycosphere 15: 1455–1521. https://doi.org/10.1080/21501203.2024.2379476
  33. Wang, X.H., Das, K., Horman, J., Antonín, V., Antonin, V., Baghela, A., Chakraborty, D., Hembrom, M.E., Nakasone, K., Ortiz-Santana, B., Vizzini, A., Hofstetter, V. & Buyck, B. (2018) Fungal biodiversity profiles 51–60. Cryptogamie Mycologie 39: 211–257. https://doi.org/10.7872/crym/v39.iss2.2018.211
  34. Wang, Y.R., Wu, Y.D., Vlasák, J., Yuan, Y. & Dai, Y.C. (2021) Phylogenetic analysis demonstrating four new species in Megasporoporia sensu lato (Polyporales, Basidiomycota). Mycosphere 12 (1): 1012–1037. https://doi.org/10.5943/mycosphere/12/1/11
  35. White, T.J., Bruns, T., Lee, S. & Taylor, J. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis, M.A., Gelfand, D.H., Sninsky, J.J. & White, T.J. (eds.) PCR protocols: A guide to methods and applications. Academic Press, San Diego, pp. 315–322. https://doi.org/10.1016/B978-0-12-372180-8.50042-1
  36. Wu, F., Man, X.W., Tohtirjap, A. & Dai, Y.C. (2022a) A comparison of polypore fungal and species composition in forest ecosystems of China, North America, and Europe. Forest Ecosystems 9: 100051. https://doi.org/10.1016/j.fecs.2022.100051
  37. Wu, F., Yuan, Y., Chen, J.J., Cui, B.K., Zhou, M. & Dai, Y.C. (2020) Terrestriporiaceae fam. nov., a new family of Russulales (Basidiomycota). Mycosphere 11: 2755–2766. https://doi.org/10.5943/mycosphere/11/1/21
  38. Wu, F., Zhou, L.W., Vlasák, J. & Dai, Y.C. (2022b) Global diversity and systematics of Hymenochaetaceae with poroid hymenophore. Fungal Diversity 113: 1–192. https://doi.org/10.1007/s13225-021-00496-4
  39. Yuan, Y., Bian, L.S., Wu, Y.D., Chen, J.J., Wu, F., Liu, H.G., Zeng, G.Y. & Dai, Y.C. (2023) Species diversity of pathogenic wood-rotting fungi (Agaricomycetes, Basidiomycota) in China. Mycology 14: 204–226. https://doi.org/10.1080/21501203.2023.2238779
  40. Zhao, C.L., Chen, H., He, S.H. & Dai, Y.C. (2016) Radulotubus resupinatus gen. et sp. nov. with a poroid hymenophore in Pterulaceae (Agaricales, Basidiomycota). Nova Hedwigia 103: 265–278. https://doi.org/10.1127/nova_hedwigia/2016/0350
  41. Zhao, C.L., Qu, M.H., Huang, R.X. & Karunarathna, S.C. (2023a) Multi‐Gene Phylogeny and taxonomy of the wood‐rotting fungal genus Phlebia sensu lato (Polyporales, Basidiomycota). Journal of Fungi 9: 320. https://doi.org/10.3390/jof9030320
  42. Zhao, H., Nie, Y., Zong, T.K., Wang, K., Lv, M.L., Cui, Y.J., Tohtirjap, A., Chen, J.J., Zhao, C.L., Wu, F., Cui, B.K., Yuan, Y., Dai, Y.C. & Liu, X.Y. (2023b) Species diversity, updated classification and divergence times of the phylum Mucoromycota. Fungal Diversity 123: 49–157. https://doi.org/10.1007/s13225-023-00525-4
  43. Zhao, H., Wu, Y.D., Yang, Z.R., Liu, H.G., Wu, F., Dai, Y.C. & Yuan, Y. (2024) Polypore funga and species diversity in tropical forest ecosystems of Africa, America and Asia, and a comparison with temperate and boreal regions of the Northern Hemisphere. Forest Ecosystems 11: 100200. https://doi.org/10.1016/j.fecs.2024.100200
  44. Zhou, H.M., Gu, Z.R. & Zhao, C.L. (2024) Molecular phylogeny and morphology reveal a new species of Asterostroma from Guizhou Province, China. Phytotaxa 634 (1): 1–15. https://doi.org/10.11646/phytotaxa.635.2.7

How to Cite

Chen, D., Zhang, S., Yang, R., Li, C., Yan, M., Dong, J. & Zhao, C. (2025) Molecular phylogeny and morphology reveal a new species of Radulomyces (Radulomycetaceae, Agaricales) from Southwestern China. Phytotaxa 696 (3): 205–216. https://doi.org/10.11646/phytotaxa.696.3.1