Skip to main content Skip to main navigation menu Skip to site footer
Responsive image
Issue: Vol. 693 No. 2: 12 Mar. 2025
Type: Article
Published: 2025-03-12
DOI: 10.11646/phytotaxa.693.2.1
Page range: 113-132
Abstract views: 530
PDF downloaded: 15

Description of two new species of Pseudosperma in central Italy

Via Yuri Gagarin 2, 06012 Città Di Castello, Perugia, Italy
CNR-IBBR: Institute of Biosciences and BioResources, Perugia Division, Italy
Via Martiri di via Fani 22, I-61024 Mombaroccio (PU), Italy
CNR-IBBR: Institute of Biosciences and BioResources, Perugia Division, Italy
CNR-IBBR: Institute of Biosciences and BioResources, Perugia Division, Italy
Agaricales Basidiomycota fungal diversity molecular taxonomy new taxa taxonomy Fungi

Abstract

Two new species belonging to the genus Pseudosperma (Inocybaceae) are reported. The basidiocarps were collected from two distinct environments in the Umbria region of central Italy. Pseudosperma cupreum sp. nov. was found in a riparian habitat dominated by Populus sp., Salix sp., and Alnus sp. trees, whereas Pseudosperma franchettii sp. nov. was found in a park with both coniferous and broad-leaved trees. Detailed macro- and micro-morphological descriptions, along with comparisons to similar species, are reported. Phylogenetic analyses based on the sequences of internal transcribed spacer region of rDNA (ITS), parts of the large subunit of rDNA (LSU) and of the second largest subunit of RNA polymerase II (rpb2), confirmed that both species form distinct, well-supported clades, clearly separating them from previously sequenced Pseudosperma specimens.

References

  1. Ainsworth, A.M., Fortey, R., Biketova, A.Y. & Suz, L.M. (2024) Fungi (excluding lichens), Chapter 5. In: Farjon, A. (Ed.) The Natural History of Blenheim’s High Park. Pelagic Press, London, 424 pp. https://doi.org/10.53061/OZOM2670
  2. Aïgnon, H.L., Fan, Y.-G. & Matheny, P.B. (2024) Pseudosperma brunneopilosum, a new species of Inocybaceae from West Africa. Nordic Journal of Botany, e04470. https://doi.org/10.1111/njb.04470
  3. Alvarado, P., Manjón, J.L., Matheny, P.B. & Esteve-Raventós, F. (2010) Tubariomyces, a new genus of Inocybaceae from the Mediterranean region. Mycologia 102: 1389–1397. https://doi.org/10.3852/10-041
  4. Angelini, P., Arcangeli, A., Bistocchi, G., Rubini, A., Venanzoni, R. & Perini, C. (2017) Current knowledge of Umbrian macrofungi (central Italy). Plant Biosystems—An International Journal Dealing with all Aspects of Plant Biology 151: 915–923. https://doi.org/10.1080/11263504.2016.1265609
  5. Angelini, P., Bistocchi, G., Arcangeli, A., Rubini, A. & Venanzoni, R. (2016) Inventory, diversity and communities of macrofungi in the Collestrada forest (Umbria, central Italy). Plant Biosystems—An International Journal Dealing with all Aspects of Plant Biology 150: 1096–1105. https://doi.org/10.1080/11263504.2015.1108939
  6. Bandini, D. & Oertel, B. (2020) Three new species of the genus Pseudosperma (Inocybaceae). Czech Mycology 72: 221–250. https://doi.org/10.33585/cmy.72205
  7. Bandini, D., Oertel, B. & Eberhardt, U. (2022) Noch mehr risspilze (3): einundzwanzig neue arten der familie inocybaceae. Mycologia Bavarica 22: 31–138.
  8. Bandini, D., Oertel, B. & Eberhardt, U. (2023) Even more fibre-caps (4): Fourteen new species of the family Inocybaceae. Mycologia Bavarica 23: 1–50.
  9. Bandini, D., Oertel, B. & Eberhardt, U. (2024) Noch mehr Risspilze (5): Elf neue Arten der Familie Inocybaceae und Epitypifizierung von Pseudosperma rimosum. Mycologia Bavarica 24: 1–52.
  10. Bau, T. & Fan, Y-G. (2018) Three new species of Inocybe sect. Rimosae from China. Mycosystema 37: 693–702.
  11. Bougher, N.L., Matheny, P.B. & Gates, G.M. (2012) Five new species and records of Inocybe (Agaricales) from temperate and tropical Australia. Nuytsia 22: 57–74. https://doi.org/10.58828/nuy00630
  12. Bresadola, G. (1905) Hymenomycetes novi vel minus cogniti. Annales Mycologici 3: 161.
  13. Bulliard, P. (1791) Histoire des champignons de la France, ou traité élemèntaire, Renfermant dans un ordre méthodique les Descriptions et les Figures des Champignons qui croissent naturellement en France. Vol. 1. Paris, 368 pp.
  14. Carbone, M., Galeotti, G.B., Lezzi, T., Athanasiadis, A. & Alvarado, P. (2021) Warcupia cupulata una nuova specie a forma di coppa nel genere cleistotecioide Warcupia (Otideaceae, Pezizales). Ascomycete.org 13 (4): 145–156.
  15. Carteret, X. & Reumaux, P. (2015) Miettes sur les inocybes (8th série). Inocybes jaunes ou jaunâtres. Bulletin de la Société Mycologique de France 131: 1–96.
  16. Cervini, M. (2022) Pseudosperma minutulum. Index Fungorum 520: 1.
  17. Cervini, M. (2023) Presentazione di Pseudosperma minutulum e prima segnalazione per il territorio italiano di Inocybe undinea. Rivista di Micologia 66: 117–135.
  18. Cervini, M., Bizio, E. & Alvarado, P. (2020) Quattro nuove specie italiane del genere Pseudosperma (Inocybaceae) con odore di miele. Rivista di Micologia 63: 3–36.
  19. Dessì, P. & Contu, M. (1999) Paxillus ammoniavirescens spec. nov. with notes on the genus Paxillus in Sardinia. Micologia e Vegetazione Mediterranea 13: 121–130.
  20. Edwards, K., Johnstone, C. & Thompson, C. (1991) A simple and rapid method for the preparation of plant genomic DNA for PCR analysis. Nucleic Acids Research 19: 1349. https://doi.org/10.1093/nar/19.6.1349
  21. Fries, E.M. (1821) Systema Mycologicum. Lund, 520 pp.
  22. Fries, E.M. (1863) Monographia Hymenomycetum Sueciae. C.A. Leffler, Uppsala, 355 pp.
  23. Gardes, M. & Bruns, T.D. (1993) ITS primers with enhanced specificity for basidiomycetes—application to the identification of mycorrhizae and rusts. Molecular Ecology 2: 113–118. https://doi.org/10.1111/j.1365-294X.1993.tb00005.x
  24. Gelardi, M., Simonini, G., Ercole, E., Davoli, P. & Vizzini, A. (2015) Cupreoboletus (Boletaceae, Boletineae), a new monotypic genus segregated from Boletus sect. Luridi to reassign the Mediterranean species B. poikilochromus. Mycologia 107: 1254–1269. https://doi.org/10.3852/15-070
  25. Jabeen, S., Bashir, H. & Khalid, A.N. (2021) Pseudosperma albobrunneum sp. nov. from coniferous forests of Pakistan. Mycotaxon 136: 361–372. https://doi.org/10.5248/136.361
  26. Jargeat, P., Moreau, P.A., Gryta, H., Chaumeton, J.P. & Gardes, M. (2016) Paxillus rubicundulus (Boletales, Paxillaceae) and two new Alder-specific ectomycorrhizal species, P. olivellus and P. adelphus, from Europe and North Africa. Fungal Biology 120: 711–728. https://doi.org/10.1016/j.funbio.2016.02.008
  27. Jülich, W. (1981) Higher taxa of basidiomycetes. Bibliotheca Mycologica 85: 1–485.
  28. Katoh, K. & Standley, D.M. (2013) MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Molecular Biology and Evolution 30: 772–780. https://doi.org/10.1093/molbev/mst010
  29. Kauffman, C.H. (1924) Inocybe. North American Flora 10: 227–260.
  30. Kaygusuz, O., Bandini, D., Knudsen, H. & Türkekul, İ. (2023) Pseudosperma pamukkalense (Inocybaceae: Agaricomycetes), a new species from Turkey. Phytotaxa 599: 225–238. https://doi.org/10.11646/phytotaxa.599.4.2
  31. Kaygusuz, O., Bandini, D., Rühl, A., Sarawi, S., Yorun, N.S. & Piepenbring, M. (2024) Five new species of Pseudosperma (Inocybaceae, Agaricales) from Benin and Turkey based on morphological characteristics and phylogenetic evidence. Mycological Progress 23: 1–24. https://doi.org/10.1007/s11557-024-01964-2
  32. Kühner, R. (1980) Les grandes lignes de la classification des Agaricales, Plutéales, Tricholomatales (suite et fin). Bulletin Mensuel de la Société Linéenne de Lyon 49: 411–446. https://doi.org/10.3406/linly.1980.10439
  33. Kühner, R. (1988) Diagnoses de quelques noveaux Inocybes récoltés en zone alpine de la Vanoise (Alpes françaises). Documents Mycologiques 19: 22.
  34. Kuyper, T.W. (1986) A revision of the genus Inocybe in Europe. I. Subgenus Inosperma and the smooth-spored species of subgenus Inocybe. Persoonia, Suppl. 3: 1–247.
  35. Lange, J.E. (1917) Studies in the Agarics of Denmark. Part III. Pluteus. Collybia. Inocybe. Dansk Botanisk Arkiv 2: 1–50. https://doi.org/10.1007/978-3-662-42454-4_10
  36. Larsson, E., Ryberg, M., Moreau, P-A., Delcuse Mathiesen, Å. & Jacobsson, S. (2009) Taxonomy and evolutionary relationships within species of section Rimosae (Inocybe) based on ITS, LSU and mtLSU sequence data. Persoonia 23: 86–98. https://doi.org/10.3767/003158509X475913
  37. Latha, K.D., Haridev, P., Raj, K.A. & Manimohan, P. (2023) Pseudosperma indicum sp. nov. (Inocybaceae, Agaricales) from India. Phytotaxa 620: 47–58. https://doi.org/10.11646/phytotaxa.620.1.4
  38. Levinson, G. & Gutman, G.A. (1987) Slipped-strand mispairing: A major mechanism for DNA sequence evolution. Molecular Biology and Evolution 4: 203–221. https://doi.org/10.1093/oxfordjournals.molbev.a040442
  39. Mao, N., Xu, Y.Y., Zhao, T.Y., Lv, J.C. & Fan, L. (2022) New Species of Mallocybe and Pseudosperma from North China. Journal of Fungi 8: 256, 1–17. https://doi.org/10.3390/jof8030256
  40. Matheny, P.B. (2005) Improving phylogenetic inference of mushrooms with RPB1 and RPB2 nucleotide sequences (Inocybe; Agaricales). Molecular Phylogenetics and Evolution 35: 1–20. https://doi.org/10.1016/j.ympev.2004.11.014
  41. Matheny, P.B., Aime, M.C., Bougher, N.L., Buyck, B., Desjardin, D.E., Horak, E., Kropp, B.R., Lodge, D.J., Soytong, K., Trappe, J.M. & Hibbett, D.S. (2009) Out of the palaeotropics? Historical biogeography and diversification of the cosmopolitan ectomycorrhizal mushroom family Inocybaceae. Journal of Biogeography 36: 577–592. https://doi.org/10.1111/j.1365-2699.2008.02055.x
  42. Matheny, P.B., Aime, M.C., Smith, M.E. & Henkel, T.W. (2012) New species and reports of Inocybe (Agaricales) from Guyana. Kurtziana 37: 23–39.
  43. Matheny, P.B. & Bougher, N.L. (2006) The new genus Auritella from Africa and Australia (Inocybaceae, Agaricales): molecular systematics, taxonomy and historical biogeography. Mycological Progress 5: 2–17. https://doi.org/10.1007/s11557-005-0001-8
  44. Matheny, P.B. & Bougher, N.L. (2017) Fungi of Australia: Inocybaceae. CSIRO Publishing, Clayton South, 552 pp.
  45. Matheny, P.B., Curtis, J.M., Hofsetter, V., Aime, M.C., Moncalvo, J.M., Ge, Z.W., Yang, Z.L., Slot, J.C., Ammirati, J.F., Baroni, T.J., Bougher, N.L., Hughes, K.W., Lodge, D.J., Kerrigan, R.W., Seidl, M.T., Aanen, D.K., DeNitis, M., Daniele, G., Desjardin, D.E., Kropp, B.R., Norvell, L.L., Parker, A., Vellinga, E.C., Vilgalys, R. & Hibbett, D.S. (2006) Major clades of Agaricales: a multilocus phylogenetic overview. Mycologia 98: 982–995. https://doi.org/10.3852/mycologia.98.6.982
  46. Matheny, P.B., Hobbs, A.M. & Esteve-Raventós, F. (2020) Genera of Inocybaceae: New skin for the old ceremony. Mycologia 112: 83–120. https://doi.org/10.1080/00275514.2019.1668906
  47. Matheny, P.B. & Kudzma, L.V. (2019) New species of Inocybe (Inocybaceae) from eastern North America. The Journal of the Torrey Botanical Society 146: 213–235. https://doi.org/10.3159/TORREY-D-18-00060.1
  48. Matheny, P.B., Kudzma, L.V., Graddy, M.G., Mardini, S.M., Noffsinger, C.R., Swenie, R.A., Walker, N.C., Campagna, S.R., Halling, R., Lebeuf, R., Kuo, M., Lewis, D.P., Smith, M.E., Tabassum, M., Trudell, S.A. & Vauras, J. (2023) A phylogeny for North American Mallocybe (Inocybaceae) and taxonomic revision of eastern North American taxa. Fungal Systematics and Evolution 12: 153–202. https://doi.org/10.3114/fuse.2023.12.09
  49. Murrill, W.A. (1916) North American Flora. Vol. 9. New York Botanic Garden, 542 pp.
  50. Naseer, A., Jabeen, S., Ashfaq, A., Akbar, M., Hussain, S.I. & Khalid, A.N. (2023) Pseudosperma quercinum sp. nov. (Inocybaceae) from the Himalayan forests of Pakistan. Phytotaxa 622: 260–270. https://doi.org/10.11646/phytotaxa.622.4.3
  51. Orton, P.D. (1960) New check list of British agarics and boleti: Part III. Notes on genera and species in the list. Trans. Br. mycol. Soc. 43: 159–439. https://doi.org/10.1016/S0007-1536(60)80065-4
  52. Pegler, D.N. (1983) Agaric Flora of the Lesser Antilles. Kew Bulletin, Additional Series 9: 1–668.
  53. Persoon, C.H. (1801) Synopsis Methodica Fungorum. Part 2. Göttingen, pp. 241–706.
  54. Quélet, L. (1888) Flore mycologique de la Francee des pays limitrophes. Octave Doin Editeur, Paris, 492 pp.
  55. Ryberg, M., Larsson, E. & Jacobsson, S. (2010) An evolutionary perspective on morphological and ecological characters in the mushroom family Inocybaceae (Agaricomycotina, Fungi). Molecular Phylogenetics and Evolution 55: 431–442. https://doi.org/10.1016/j.ympev.2010.02.011
  56. Saba, M., Haelewaters, D., Pfister, D.H. & Khalid, A.N. (2020) New species of Pseudosperma (Agaricales, Inocybaceae) from Pakistan revealed by morphology and multi-locus phylogenetic reconstruction. MykoKeys 69: 1–31. https://doi.org/10.3897/mycokeys.69.33563
  57. Saccardo, P.A. (1888) Sylloge Fungorum. Vol. 6. 928 pp.
  58. Saccardo, P.A. (1891) Sylloge Fungorum. Vol. 9. 1141 pp.
  59. Sanna, M., Mua, A., Porcu, G., Casula, M., Rinaldi, A.C., Mifsud, S. & Garradino-Benavent, I. (2024) Pseudosperma calciphilum (Inocybaceae), a new Mediterranean species from Sardinia (Italy), Malta, and Valencia (Spain). Phytotaxa 633: 253–264. https://doi.org/10.11646/phytotaxa.633.3.5
  60. Singer, R. (1949) The Agaricales (Mushrooms) in modern taxonomy. Lilloa 22: 1–584.
  61. Stamatakis, A. (2014) RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 30: 1312–1313. https://doi.org/10.1093/bioinformatics/btu033
  62. Vaidya, G., Lohman, D.J. & Meier, R. (2011) SequenceMatrix: concatenation software for the fast assembly of multi‐gene datasets with character set and codon information. Cladistics 27: 171–180. https://doi.org/10.1111/j.1096-0031.2010.00329.x
  63. Vilgalys, R. & Hester, M. (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. Journal of Bacteriology 172: 4238–4246. https://doi.org/10.1128/jb.172.8.4238-4246.1990
  64. Vizzini, A., Della Maggiora, M., Tolaini, F. & Ercole, E. (2013) A new cryptic species in the genus Tubariomyces (Inocybaceae, Agaricales). Mycological Progress 12: 375–381. https://doi.org/10.1007/s11557-012-0843-9
  65. Wagensommer, R.P., Bistocchi, G., Arcangeli, A., Rubini, A., Perini, C., Venanzoni, R. & Angelini, P. (2018) An assessment of red list data for the Pezizomycotina (Ascomycota): Umbria (Italy) as a test case. Plant Biosystems 152: 1329–1337. https://doi.org/10.1080/11263504.2018.1448015
  66. White, T.J., Bruns, T.D., Lee, S.B. & Taylor, J.W. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis, M.A., Gelfand, D.H., Sninsky, J.J. & White, T.J. (Eds.) PCR Protocols: A Guide to Methods and Applications. Academic Press, New York, pp. 315–322. https://doi.org/10.1016/B978-0-12-372180-8.50042-1
  67. Yu, W.J., Chang, C., Qin, L.W., Zeng, N.K., Wang, S.X. & Fan, Y.G. (2020) Pseudosperma citrinostipes (Inocybaceae), a new species associated with Keteleeria from southwestern China. Phytotaxa 450: 8–16. https://doi.org/10.11646/phytotaxa.450.1.2
  68. Zhao, L.N., Yu, W.J., Deng, L.S., Hu, J.H., Ge, Y.P., Zeng, N.K. & Fan, Y.G. (2022) Phylogenetic analyses, morphological studies, and muscarine detection reveal two new toxic Pseudosperma (Inocybaceae, Agaricales) species from tropical China. Mycological Progress 21 (75): 1–25. https://doi.org/10.21203/rs.3.rs-1572469/v1

How to Cite

Galeotti, G.B., Rubini, A., Para, R., Riccioni, C. & Belfiori, B. (2025) Description of two new species of Pseudosperma in central Italy. Phytotaxa 693 (2): 113–132. https://doi.org/10.11646/phytotaxa.693.2.1