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Type: Article
Published: 2024-12-18
Page range: 278-288
Abstract views: 50
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Taxonomic and phylogenetic characterizations reveal two new species and a new record of Simplicillium (Cordycipitaceae, Hypocreales) from the Mayao River Valley, Guizhou, China

Center for Mycomedicine Research, Basic Medical School, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, Guizhou, PR China. Institute of Fungus Resources, Department of Ecology, College of Life Sciences, Guizhou University, Guiyang 550025, Guizhou, PR China. Key Laboratory of Microbio and Infectious Disease Prevention & Control in Guizhou Province, Guiyang 550025, Guizhou, PR China.
Center for Mycomedicine Research, Basic Medical School, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, Guizhou, PR China. College of Pharmacy, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, Guizhou, PR China.
Center for Mycomedicine Research, Basic Medical School, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, Guizhou, PR China. College of Pharmacy, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, Guizhou, PR China.
Center for Mycomedicine Research, Basic Medical School, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, Guizhou, PR China. Key Laboratory of Microbio and Infectious Disease Prevention & Control in Guizhou Province, Guiyang 550025, Guizhou, PR China.
Institute of Fungus Resources, Department of Ecology, College of Life Sciences, Guizhou University, Guiyang 550025, Guizhou, PR China
entomopathogenic fungi morphological phylogenetic analysis Simplicillium valley Fungi

Abstract

Simplicillium species are widely distributed and commonly found on various substrates or as a fungicolous fungi. In this research, three arthropod-associated specimens were collected. Two new species, Simplicillium neocoleopterorum and S. vallense, and a new record of S. formicae in China, were isolated, established and described according to a multilocus phylogenetic analysis and their morphological characteristics.

References

  1. Castlebury, L.A., Rossman, A.Y., Sung, G.H., Hyten, A.S. & Spatafora, J.W. (2004) Multigene phylogeny reveals new lineage for Stachybotrys chartarum, the indoor air fungus. Mycological Research 108: 864–872. https://doi.org/10.1017/S0953756204000607
  2. Chen, W.H., Liu, C., Han, Y.F., Liang, J.D., Tian, W.Y. & Liang, Z.Q. (2019) Three novel insect-associated species of Simplicillium (Cordycipitaceae, Hypocreales) from Southwest China. MycoKeys 58: 83–102. https://doi.org/10.3897/mycokeys.58.37176
  3. Chen, W.H., Han, Y.F., Liang, J.D. & Liang, Z.Q. (2021) Taxonomic and phylogenetic characterizations reveal four new species of Simplicillium (Cordycipitaceae, Hypocreales) from Guizhou, China. Scientific Reports 11: 15300. https://doi.org/10.1038/s41598-021-94893-z
  4. Chen, W., Liang, J., Ren, X., Zhao, J., Han, Y. & Liang, Z. (2022) Multigene phylogeny, phylogenetic network, and morphological characterizations reveal four new arthropod-associated Simplicillium species and their evolutional relationship. Frontiers in Microbiology 13: 950773. https://doi.org/10.3389/fmicb.2022.950773
  5. Chuang, W.Y., Lin, Y.C., Shrestha, B., Luangsa-ard, J.J., Stadler, M., Tzean, S.S., Wu, S., Ko, C.C., Hsieh, S.Y., Wu, M.L., Wang, S.C., Shen, T.L. & Ariyawansa, H.A. (2024) Phylogenetic diversity and morphological characterization of cordycipitaceous species in Taiwan. Studies in Mycology 109: 1–56. https://doi.org/10.3114/sim.2024.109.01
  6. Crous, P.W., Luangsa-ard, J.J., Wingfield, M.J., Carnegie, A.J., Hernández-Restrepo, M., Lombard, L., Roux, J., Barreto, R.W., Baseia, I.G., Cano-Lira, J.F., Martín, M.P., Morezova, O.V., Stchigel, A.M., Summerell, B.A., Brandrud, T.E., Dima, B., Garcia, D., Giraldo, A., Guarro, J., Gusmão, L.F.P., Khamsuntorn, P., Noordeloos, M.E., Nuankaew, S., Pinruan, U., Rodríguez-Andrade, E., Souza-Motta, C.M., Thangavel, R., van lperen, A.L., Abreu, V.P., Accioly, T., Alves, J.L., Andrade, J.P., Bahram, M., Baral, H.O., Barbier, E., Barnes, C.W., Bendiksen, E., Bernard, E., Bezerra, J.D.P., Bezerra, J.L., Bizio, E., Blair, J.E., Bulyonkova, T.M., Cabral, T.S., Caiafa, M.V., Cantillo, T., Colmán, A.A., Conceição, L.B., Cruz, S., Cunha, A.O.B., Darveaux, B.A., da Silva, A.L., da Sliva, G.A., da Sliva, G.M., da Sliva, R.M.F., de Oliveira, R.J.V., Oliveira, R.L., De Souza, J.T., Dueñas, M., Evans, H.C., Epifani, F., Felipe, M.T.C., Fernández-López, J., Ferreira, B.W., Fugueiredo, C.N., Filippova, N.V., Flores, J.A., Gené, J., Ghorbani, G., Gibertoni, T.B., Glushakova, A.M., Healy, R., Huhndorf, S.M., Iturrieta-González, I., Javan-Nikkhah, M., Juciano, R.F., Jurjević, Ž., Kachalkin, A.V., Keochanpheng, K., Krisai-Greilhuber, I., Li, Y.C., Lima, A.A., Machado, A.R., Madrid, H., Magalhães, O.M.C., Marbach, P.A.S., Melanda, G.C.S., Miller, A.N., Mongkolsamrit, S., Nascimento, R.P., Oliveira, T.G.L., Ordoñez, M.E., Orzes, R., Palma, M.A., Pearce, C.J., Pereira, O.L., Perrone, G., Peterson, S.W., Pham, T.H.G., Piontelli, E., Pordel, A., Quijada, L., Raja, H.A., Rosas de Paz, E., Ryvarden, L,, Saitta, A., Salcede, S.S., Sandoval-Denis, M., Santos, T.A.B., Seifert, K.A., Silva, B.D.B., Smith, M.E., Soares, A.M., Sommai, S., Sousa, J.O., Suetrong, S., Susca, A., Tedersoo, L., Telleria, M.T., Thanakitpipattana, D., Valenzuela-Lopez, N., Visagie, C.M., Zapata, M. & Groenewald, J.Z. (2018) Fungal Planet description sheets: 785–867. Persoonia 41: 238–417. https://doi.org/10.3767/persoonia.2018.41.12
  7. Crous, P.W., Cowan, D.A., Maggs-Kölling, G., Yilmaz, N., Thangavel, R., Wingfield, M.J., Noordeloos, M.E., Dima, B., Brandrud, T.E., Jansen, G.M., Morozova, O.V., Vila, J., Shivas, R.G., Tan, Y.P., Bishop-Hurley, S., Lacey, E., Marney, T.S., Larsson, E., Le Floch, G., Lombard, L., Nodet, P., Hubka, V., Alvarado, P., Berraf-Tebbal, A., Reyes, J.D., Delgado, G., Eichmeier, A., Jordal, J.B., Kachalkin, A.V., Kubátová, A., Maciá-Vicente, J.G., Malysheva, E.F., Papp, V., Rajeshkumar, K.C., Sharma, A., Spetik, M., Szabóová, D., Tomashevskaya, M.A., Abad, J.A., Abad, Z.G., Alexandrova, A.V., Anand, G., Arenas, F., Ashtekar, N., Balashov, S., Bañares, Á., Baroncelli, R., Bera, I., Biketova, A.Y., Blomquist, C.L., Boekhout, T., Boertmann, D., Bulyonkova, T.M., Burgess, T.I., Carnegie, A.J., Cobo-Diaz, J.F., Corriol, G., Cunnington, J.H., da Cruz, M.O., Damm, U., Davoodian, N., de A. Santiago, A.L.C.M., Dearnaley, J., de Freitas, L.W.S., Dhileepan, K., Dimitrov, R., Di Piazza, S., Fatima, S., Fuljer, F., Galera, H., Ghosh, A., Giraldo, A., Glushakova, A.M., Gorczak, M., Gouliamova, D.E., Gramaje, D., M. Groenewald, M., Gunsch, C.K., Gutiérrez, A., Holdom, D., Houbraken, J., Ismailov, A.B., Istel, Ł., Iturriaga, T., Jeppson, M., Jurjević, Ž., Kalinina, L.B., Kapitonov, V.I., Kautmanová, I., Khalid, A.N., Kiran, M., Kiss, L., Kovács, Á., Kurose, D., Kušan, I., Lad, S., Læssøe, T., Lee, H.B., Luangsa-ard, J.J., Lynch, M., Mahamedi, A.E., Malysheva, V.F., Mateos, A., Matočec, N., Mešić, A., Miller, A.N., Mongkolsamrit, S., Moreno, G., Morte, A., Mostowfizadeh-Ghalamfarsa, R., Naseer, A., Navarro-Ródenas, A., Nguyen, T.T.T., Noisripoom, W., Ntandu, J.E., Nuytinck, J., Ostrý, V., Pankratov, T.A., Pawłowska, J., Pecenka, J., Pham, T.H.G., Polhorský, A., Pošta, A., Raudabaugh, D.B., Reschke, K., Rodríguez, A., Romero, M., Rooney-Latham, S., Roux, J., Sandoval-Denis, M., Smith, M.T., Steinrucken, T.V., Svetasheva, T.Y., Tkalčec, Z., van der Linde, E.J., Vegte, M.V.D., Vauras, J., Verbeken, A., Visagie, C.M., Vitelli, J.S., Volobuev, S.V., Weill, A., Wrzosek, M., Zmitrovich, I.V., Zvyagina, E.A. & Groenewald, J.Z. (2021) Fungal Planet description sheets: 1182–1283. Persoonia 46: 313–528. https://doi.org/10.3767/persoonia.2021.46.11
  8. Drummond, A. & Rambaut, A. (2007) BEAST: Bayesian evolutionary analysis by sampling trees. BMC Evolutionary Biology 7: 214. https://doi.org/10.1186/1471-2148-7-214
  9. Gomes, A.A., Pinho, D.B., Cardeal, Z.L., Menezes, H.C., De Queiroz, M.V. & Pereira, O.L. (2018) Simplicillium coffeanum, a new endophytic species from Brazilian coffee plants, emitting antimicrobial volatiles. Phytotaxa 333 (2): 188–198. https://doi.org/10.11646/phytotaxa.333.2.2
  10. Jeewon, R. & Hyde, K.D. (2016) Establishing species boundaries and new taxa among fungi: recommendations to resolve taxonomic ambiguities. Mycosphere 7: 1669–1677. https://doi.org/10.5943/mycosphere/7/11/4
  11. Kalyaanamoorthy, S., Minh, B.Q., Wong, T.K., Von Haeseler, A. & Jermiin, L.S. (2017) ModelFinder: fast model selection for accurate phylogenetic estimates. Nature Methods 14: 587–589. https://doi.org/10.1038/nmeth.4285
  12. Katoh, K. & Standley, D.M. (2013) MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Molecular Biology and Evolution 30 (4): 772–780. https://doi.org/10.1093/molbev/mst010
  13. Leplat, J., Francois, A. & Bousta, F. (2021) Simplicillium pech-merlensis, a new fungal species isolated from the air of the Pech-Merle show cave. Phytotaxa 521 (2): 80–94. https://doi.org/10.11646/phytotaxa.521.2.2
  14. Liang, J.D., Han, Y.F., Zhang, J.W., Du, W., Liang, Z.Q. & Li, Z.Z. (2011) Optimal culture conditions for keratinase production by a novel thermophilic Myceliophthora thermophila strain GZUIFR-H49-1. Journal of Applied Microbiology 110: 871–880. https://doi.org/10.1111/j.1365-2672.2011.04949.x
  15. Liu, F. & Cai, L. (2012) Morphological and molecular characterization of a novel species of Simplicillium from China. Cryptogamie, Mycologie 33 (2): 137–145. https://doi.org/10.7872/crym.v33.iss2.2012.137
  16. Nonaka, K., Kaifuchi, S., Ōmura, S. & Masuma, R. (2013) Five new Simplicillium species (Cordycipitaceae) from soils in Tokyo, Japan. Mycoscience 54 (1): 42–53. https://doi.org/10.1016/j.myc.2012.07.002
  17. Okane, I., Nonaka, K., Kurihara, Y., Abe, J.P. & Yamaoka, Y. (2020) A new species of Leptobacillim, L. symbioticum, isolated from mites and sori of soybean rust. Mycoscience 61 (4): 165–171. https://doi.org/10.1016/j.myc.2020.04.006
  18. Quaedvlieg, W., Binder, M., Groenewald, J.Z., Summerell, B.A., Carnegie, A.J., Burgess, T.I. & Crous, W. (2014) Introducing the consolidated species concept to resolve species in the Teratosphaeriaceae. Persoonia 33: 1–40. https://doi.org/10.3767/003158514X681981
  19. Rakotonirainy, M.S., Cariou, M.L., Brygoo, Y. & Riba, G. (1994) Phylogenetic relationships within the genus Metarhizium based on 28S rRNA sequences and isozyme comparison. Mycological Research 98 (2): 225–230. https://doi.org/10.1016/S0953-7562(09)80190-1
  20. Ronquist, F., Teslenko, M., van der Mark, P., Ayres, D.L., Darling, A., Höhna, S., Larget, B., Liu, L., Suchard, M.A. & Huelsenbeck, J.P. (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology 61: 539–542. https://doi.org/10.1093/sysbio/sys029
  21. Spatafora, J.W., Sung, G.H., Sung, J.M., Hywel-Jones, N.L. & White, J.F. (2007) Phylogenetic evidence for an animal pathogen origin of ergot and the grass endophytes. Molecular Ecology 16: 1701–1711. http://doi.org/10.1111/j.1365-294X.2007.03225.x
  22. Tamura, K., Stecher, G., Peterson, D., Filipski, A. & Kumar, S. (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution 30: 2725–2729. https://doi.org/10.1093/molbev/mst197
  23. Vaidya, G., Lohman, D.J. & Meier, R. (2011) SequenceMatrix: concatenation software for the fast assembly of multi‐gene datasets with character set and codon information. Cladistics 27 (2): 171–180. https://doi.org/10.1111/j.1096-0031.2010.00329.x
  24. Wang, Y.B., Wang, Y., Fan, Q., Duan, D.E., Zhang, G.D., Dai, R.Q., Dai, Y.D., Zeng, W.B., Chen, Z.H., Li, D.D., Tang, D.X., Xu, Z.H., Sun, T., Nguyen, T., Tran, N., Dao, V., Zhang, C.M., Huang, L.D., Liu, Y.J., Zhang, X.M., Yang, D.R., Sanjuan, T., Liu, X.Z., Yang, Z.L. & Yu, H. (2020) Multigene phylogeny of the family Cordycipitaceae (Hypocreales): new taxa and the new systematic position of the Chinese cordycipitoid fungus Paecilomyces hepiali. Fungal Diversity 103: 1–46. https://doi.org/10.1007/s13225-020-00457-3
  25. Wei, D.P., Wanasinghe, D.N., Hyde, K.D., Mortimer, P.E., Xu, J.C., Xiao, Y.P., Bhunjun, C.S. & Toanun, C. (2019) The genus Simplicillium. MycoKeys 60: 69–92. https://doi.org/10.3897/mycokeys.60.38040
  26. White, T.J., Bruns, T., Lee, S. & Taylor, J. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis, M.A., Gelfand, D.H., Sninsky, J.J. & White, T.J. (Eds.) PCR protocols: a guide to methods and applications. Academic Press, New York, pp. 315–322. https://doi.org/10.1016/B978-0-12-372180-8.50042-1
  27. Yan, Q.H., Ni, Q.R., Gu, W.J., Liu, H.W., Yuan, X.Y. & Sun, J.Z. (2023) Simplicillium sinense sp. nov., a novel potential pathogen of tinea faciei. Frontiers in Microbiology 14: 1156027. https://doi.org/10.3389/fmicb.2023.1156027
  28. Zare, R. & Gams, W. (2001) A revision of Verticillium section Prostrata. IV. The genera Lecanicillium and Simplicillium gen. nov. Nova Hedwigia 73: 1–50. https://doi.org/10.1127/nova.hedwigia/73/2001/1
  29. Zare, R. & Gams, W. (2008) A revision of the Verticillium fungicola species complex and its affinity with the genus Lecanicillium. Mycological Research 112 (7): 811–824. https://doi.org/10.1016/j.mycres.2008.01.019
  30. Zhang, Z.F., Liu, F., Zhou, X., Liu, X.Z., Liu, S.J. & Cai, L. (2017) Culturable mycobiota from Karst caves in China, with descriptions of 20 new species. Persoonia 39: 1–31. https://doi.org/10.3767/persoonia.2017.39.01
  31. Zhang, D., Gao, F., Jakovlić, I., Zou, H., Zhang, J., Li, W.X. & Wang, G.T. (2020 b) PhyloSuite: an integrated and scalable desktop platform for streamlined molecular sequence data management and evolutionary phylogenetics studies. Molecular Ecology Resources 20: 348–355. https://doi.org/10.1111/1755-0998.13096.
  32. Zhang, Z.F., Zhou, S.Y., Eurwilaichitr, L., Ingsriswang, S., Raza, M., Chen, Q., Zhao, P., Liu, F. & Cai, L. (2020a) Culturable mycobiota from Karst caves in China II, with descriptions of 33 new species. Fungal Diversity 106: 29–136. https://doi.org/10.1007/s13225-020-00453-7
  33. Zou, X., Liu, A.Y., Liang, Z.Q., Han, Y.F. & Yang, M. (2010) Hirsutella liboensis, a new entomopathogenic species affecting Cossidae (Lepidoptera) in China. Mycotaxon 111 (1): 39–44. https://doi.org/10.5248/111.39