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Issue: Vol. 677 No. 2: 16 Dec. 2024
Type: Article
Published: 2024-12-16
DOI: 10.11646/phytotaxa.677.2.3
Page range: 144-154
Abstract views: 199
PDF downloaded: 2

Cordyceps duyunensis, a new species from Doupeng Mount, Guizhou, China

Center for Mycomedicine Research, Basic Medical School, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, Guizhou, PR China. College of Pharmacy, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, Guizhou, PR China.
Center for Mycomedicine Research, Basic Medical School, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, Guizhou, PR China. Institute of Fungus Resources, Department of Ecology, College of Life Sciences, Guizhou University, Guiyang 550025, Guizhou, PR China. Key Laboratory of Microbio and Infectious Disease Prevention & Control in Guizhou Province, Guiyang 550025, Guizhou, PR China.
Center for Mycomedicine Research, Basic Medical School, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, Guizhou, PR China. College of Pharmacy, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, Guizhou, PR China.
Center for Mycomedicine Research, Basic Medical School, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, Guizhou, PR China. College of Pharmacy, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, Guizhou, PR China.
Center for Mycomedicine Research, Basic Medical School, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, Guizhou, PR China. Key Laboratory of Microbio and Infectious Disease Prevention & Control in Guizhou Province, Guiyang 550025, Guizhou, PR China.
Institute of Fungus Resources, Department of Ecology, College of Life Sciences, Guizhou University, Guiyang 550025, Guizhou, PR China
Entomopathogenic fungi Cordyceps morphology phylogeny new species Fungi

Abstract

A new species, Cordyceps duyunensis, which was isolated from an infected pupa, is introduced. Morphological comparisons and phylogenies analysis of a multigene dataset (SSU, ITS, RPB1, RPB2 and TEF) support the establishment of the new species. It differs from related species by its smaller phialides.

References

  1. Castlebury, L.A., Rossman, A.Y., Sung, G.H., Hyten, A.S. & Spatafora, J.W. (2004) Multigene phylogeny reveals new lineage for Stachybotrys chartarum, the indoor air fungus. Mycological Research 108: 864–872. https://doi.org/10.1017/S0953756204000607
  2. Chen, W.H., Liu, C., Han, Y.F., Liang, J.D., Tian, W.Y. & Liang, Z.Q. (2019) Three novel insect-associated species of Simplicillium (Cordycipitaceae, Hypocreales) from Southwest China. MycoKeys 58: 83–102. https://doi.org/10.3897/mycokeys.58.37176
  3. Chen, W., Liang, J., Ren, X., Zhao, J., Han, Y. & Liang, Z. (2021) Cryptic diversity of isaria-like species in Guizhou, China. Life (Basel, Switzerland) 11 (10): 1093. https://doi.org/10.3390/life11101093
  4. Chen, W.H., Liang, J.D., Ren, X.X., Zhao, J.H., Han, Y.F. & Liang, Z.Q. (2022) Species diversity of Cordyceps-like fungi in the Tiankeng karst region of China. Microbiology Spectrum 10 (5): e01975-22. https://doi.org/10.1128/spectrum.01975-22
  5. Chuang, W.Y., Lin, Y.C., Shrestha, B., Luangsa-ard, J.J., Stadler, M., Tzean, S.S., Wu, S., Ko, C.C., Hsieh, S.Y., Wu, M.L., Wang, S.C., Shen, T.L. & Ariyawansa, H.A. (2024) Phylogenetic diversity and morphological characterization of cordycipitaceous species in Taiwan. Studies in Mycology 109: 1–56. https://doi.org/10.3114/sim.2024.109.01
  6. Crous, P.W., Wingfield, M.J., Lombard, L., Roets, F., Swart, W.J., Alvarado, P., Carnegie, A.J., Moreno, G., Luangsa-ard, J., Thangavel, R., Alexandrova, A.V., Baseia, I.G., Bellanger, J.M., Bessette, A.E., Bessette, A.R., De la Peña-Lastra, S., García, D., Gené, J., Pham, T.H.G., Heykoop, M., Malysheva, E., Malysheva, V., Martín, M.P., Morozova, O.V., Noisripoom, W., Overton, B.E., Rea, A.E., Sewall, B.J., Smith, M.E., Smyth, C.W., Tasanathai, K., Visagie, C.M., Adamčík, S., Alves, A., Andrade, J.P., Aninat, M.J., Araújo, R.V.B., Bordallo, J.J., Boufleur, T., Baroncelli, R., Barreto, R.W., Bolin, J., Cabero, J., Caboň, M., Cafà, G., Caffot, M.L.H., Cai, L., Carlavilla, J.R., Chávez, R., de Castro, R.R.L., Delgat, L., Deschuyteneer, D., Dios, M.M., Domínguez, L.S., Evans, H.C., Eyssartier, G., Ferreira, B.W., Figueiredo, C.N., Liu, F., Fournier, J., Galli-Terasawa, L.V., Gil-Durán, C., Glienke, C., Gonçalves, M.F.M., Gryta, H., Guarro, J., Himaman, W., Hywel-Jones, N., Iturrieta-González, I., Ivanushkina, N.E., Jargeat, P., Khalid, A.N., Khan, J., Kiran, M., Kiss, L., Kochkina, G.A., Kolařík, M., Kubátová, A., Lodge, D.J., Loizides, M., Luque, D., Manjón, J.L., Marbach, P.A.S., Massola, Jr N.S., Mata, M., Miller, A.N., Mongkolsamrit, S., Moreau, P.A., Morte, A., Mujic, A., Navarro-Ródenas, A., Németh, M.Z., Nóbrega, T.F., Nováková, A., Olariaga, I., Ozerskaya, S.M., Palma, M.A., Petters-Vandresen, D.A.L., Piontelli, E., Popov, E.S., Rodríguez, A., Requejo, Ó., Rodrigues, A.C.M., Rong, I.H., Roux, J., Seifert, K.A., Silva, B.D.B., Sklenář, F., Smith, J.A., Sousa, J.O., Souza, H.G., De Souza, J.T., Švec, K., Tanchaud, P., Tanney, J.B., Terasawa, F., Thanakitpipattana, D., Torres-Garcia, D., Vaca, I., Vaghefi, N., van Iperen, A.L., Vasilenko, O.V., Verbeken, A., Yilmaz, N., Zamora, J.C., Zapata, M., Jurjević, Ž. & Groenewald, J.Z. (2019) Fungal Planet description sheets: 951–1041. Persoonia 43: 223–425. https://doi.org/10.3767/persoonia.2019.43.06
  7. Crous, P.W., Osieck, E.R., Shivas, R.G., Tan, Y.P., Bishop-Hurley, S.L., Esteve-Raventós, F., Larsson, E., Luangsa-ard, J.J., Pancorbo, F., Balashov, S., Baseia, I.G., Boekhout, T., Chandranayaka, S., Cowan, D.A., Cruz, R.H.S.F., Czachura, P., De la Peña-Lastra, S., Dovana, F., Drury, B., Fell, J., Flakus, A., Fotedar, R., Jurjević, Ž., Kolecka, A., Mack, J., Maggs-Kölling, G., Mahadevakumar, S., Mateos, A., Mongkolsamrit, S., Noisripoom, W., Plaza, M., Overy, D.P., Piątek, M., Sandoval-Denis, M., Vauras, J., Wingfield, M.J., Abell, S.E., Ahmadpour, A., Akulov, A., Alavi, F., Alavi, Z., Altes, A., Alvarado, P., Anand, G., Ashtekar, N., Assyov, B., Banc-Prandi, G., Barbosa, K.D., Barreto, G.G., Bellanger, J.M., Bezerra, J.L., Bhat, D.J., Bilanski, P., Bose, T., Bozok, F., Chaves, J., Costa-Rezende, D.H., Danteswari, C., Darmostuk, V., Delgado, G., Denman, S., Eichmeier, A., Etayo, J., Eyssartier, G., Faulwetter, S., Ganga, K.G.G., Ghosta, Y., Goh, J., Góis, J.S., Gramaje, D., Granit, L., Groenewald, M., Gulden, G., Gusmão, L.F.P., Hammerbacher, A., Heidarian, Z., Hywel-Jones, N., Jankowiak, R., Kaliyaperumal, M., Kaygusuz, O., Kezo, K., Khonsanit, A., Kumar, S., Kuo, C.H., Læssøe, T., Latha, K.P.D., Loizides, M., Luo, S.M., Maciá-Vicente, J.G., Manimohan, P., Marbach, P.A.S., Marinho, P., Marney, T.S., Marques, G., Martín, M.P., Miller, A.N., Mondello, F., Moreno, G., Mufeeda, K.T., Mun, H.Y., Nau, T., Nkomo, T., Okrasińska, A., Oliveira, J.P.A.F., Oliveira, R.L., Ortiz, D.A., Pawłowska, J., Pérez-De-Gregorio, M.À., Podile, A.R., Portugal, A., Privitera, N., Rajeshkumar, K.C., Rauf, I., Rian, B., Rigueiro-Rodríguez, A., Rivas-Torres, G.F., Rodriguez-Flakus, P., Romero-Gordillo, M., Saar, I., Saba, M., Santos, C.D., Sarma, P.V.S.R.N., Siquier, J.L., Sleiman, S., Spetik, M., Sridhar, K.R., Stryjak-Bogacka, M., Szczepańska, K., Taşkın, H., Tennakoon, D.S., Thanakitpipattana, D., Trovao, J., Türkekul, İ., van Iperen, A.L., van 't Hof, P., Vasquez, G., Visagie, C.M., Wingfield, B.D., Wong, P.T.W., Yang, W.X., Yarar, M., Yarden, O., Yilmaz, N., Zhang, N., Zhu, Y.N. & Groenewald, J.Z. (2023) Fungal Planet description sheets: 1478–1549. Persoonia 50 (1): 158–310. https://doi.org/10.3767/persoonia.2023.50.05
  8. Dong, Q.Y., Wang, Y., Wang, Z.Q., Tang, D.X., Zhao, Z.Y., Wu, H.J. & Yu, H. (2022) Morphology and phylogeny reveal five novel species in the genus Cordyceps (Cordycipitaceae, Hypocreales) from Yunnan, China. Frontiers in Microbiology 13: 846909. https://doi.org/10.3389/fmicb.2022.846909
  9. Drummond, A. & Rambaut, A. (2007) BEAST: Bayesian evolutionary analysis by sampling trees. BMC Evolutionary Biology 7: e214. https://doi.org/10.1186/1471-2148-7-214
  10. Duan, D.E. (2019) Taxonomy and phylogeny of Cordycipitaceae from Vietnam (Doctoral dissertation, Master’s thesis. Kunming: Yunnan University). https://cdmd.cnki.com.cn/Article/CDMD-10673-1019178333.htm
  11. Hu, J.J., Zhao, G.P., Tuo, Y.L., Dai, D., Guo, D.Z., Rao, G., Qi, Z.X., Zhang, Z.H., Li, Y. & Zhang, B. (2021) Morphology and molecular study of three new cordycipitoid fungi and its related species collected from Jilin Province, northeast China. MycoKeys 83: 161–180. https://doi.org/10.3897/mycokeys.83.72325
  12. Hyde, K.D., Tennakoon, D.S., Jeewon, R., Bhat, D.J., Maharachchikumbura, S.S.N., Rossi, W., Leonardi, M., Lee, H.B., Mun, H.Y., Houbraken, J., Nguyen, T.T.T., Jeon, S.J., Frisvad, J.C., Wanasinghe, D.N., Lücking, R., Aptroot, A., Caceres, M.E.S., Karunarathna, S.C., Hongsanan, S., Phookamsak, R., Silva, N.I., Thambugala, K.M., Jayawardena, R.S., Senanayake, I.C., Boonmee, S., Chen, J., Luo, Z.L., Phukhamsakda, C., Pereira, O.L., Abreu, V.P., Rosado, A.W.C., Buyck, B., Randrianjohany, E., Hofstetter, V., Gibertoni, T.B., Silva Soares, A.M., Plautz, Jr. H.L., Pontes Sotao, H.M., Xavier, W.K.S., Bezerra, J.D.P., Oliveira, T.G.L., Souza-Motta, C.M., Magalhaes, O.M.C., Bundhun, D., Harishchandra, D., Manawasinghe, I.S., Dong, W., Zhang, S.N., Bao, D.F., Samarakoon, M.C., Pem, D., Karunarathna, A., Lin, C.G., Yang, J., Perera, R.H., Kumar, V., Huang, S.K., Dayarathne, M.C., Ekanayaka, A.H., Jayasiri, S.C., Xiao, Y.P., Konta, S., Niskanen, T., Liimatainen, K., Dai, Y.C., Ji, X.H., Tian, X.M., Mešić, A., Singh, S.K., Phutthacharoen, K., Cai, L., Sorvongxay, T., Thiyagaraja, V., Norphanphoun, C., Chaiwan, N., Lu, Y.Z., Jiang, H.B., Zhang, J.F., Abeywickrama, P.D., Aluthmuhandiram, J.V.S., Brahmanage, R.S., Zeng, M., Chethana, T., Wei, D.P., Réblová, M., Fournier, J., Nekvindova, J., Barbosa, R.N., Felinto dos Santos, J.E., Oliveira, N.T., Li, G.J., Ertz, D., Shang, Q.J., Phillips, A.J.L., Kuo, C.H., Camporesi, E., Bulgakov, T.S., Lumyong, S., Jones, E.B.G., Chomnunti, P., Gentekaki, E., Bungartz, F., Zeng, X.Y., Fryar, S., Tkalčec, Z., Liang, J.M., Li, G.S., Wen, T.C., Singh, P.N., Gafforov, Y., Promputtha, I., Yasanthika, E., Goonasekara, I.D., Zhao, R.L., Zhao, Q., Kirk, P.M., Liu, J.K., Yan, J.Y., Mortimer, P.E., Xu, J.C. & Doilom, M.W. (2019) Fungal diversity notes 1036–1150: taxonomic and phylogenetic contributions on genera and species of fungal taxa. Fungal Diversity 96: 1–242. https://doi.org/10.1007/s13225-019-00429-2
  13. Kalyaanamoorthy, S., Minh, B.Q., Wong, T.K., Von Haeseler, A. & Jermiin, L.S. (2017) ModelFinder: fast model selection for accurate phylogenetic estimates. Nature Methods 14: 587–589. https://doi.org/10.1038/nmeth.4285.
  14. Katoh, K. & Standley, D.M. (2013) MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Molecular Biology and Evolution 30 (4): 772–780. https://doi.org/10.1093/molbev/mst010
  15. Kepler, R.M., Luangsa-ard, J.J., Hywel-Jones, N.L., Quandt, C.A., Sung, G.H., Rehner, S.A., Aime, M.C., Henkel, T.W., Sanjuan, T., Zare, R., Chen, M.J., Li, Z.Z., Rossman, A.Y., Spatafora, J.W. & Shrestha, B. (2017) A phylogenetically-based nomenclature for Cordycipitaceae (Hypocreales). IMA Fungus 8 (2): 335–353. https://doi.org/10.5598/imafungus.2017.08.02.08
  16. Li, Y.P., Chen, W.H., Han, Y.F., Liang, J.D. & Liang, Z.Q. (2020) Cordyceps yinjiangensis, a new ant-pathogenic fungus. Phytotaxa 453 (3): 284–292. https://doi.org/10.11646/phytotaxa.453.3.10
  17. Li, Z.Z., Luan, F.G., Hywel-Jones, N.L., Zhang, S.L., Chen, M.J., Huang, B., Sun, C.S., Chen, Z.A., Li, C.R., Tan, Y.J. & Dong, J.F. (2021) Biodiversity of cordycipitoid fungi associated with Isaria cicadae Miquel II: teleomorph discovery and nomenclature of chanhua, an important medicinal fungus in China. Mycosystema 40 (1): 95–107. https://doi.org/10.13346/j.mycosystema.200119
  18. Liang, Z.Q. (1991) Verification and identification of the anamorph of Cordyceps pruinosa Petch. Acta Mycologica Sinica 10: 104–107.
  19. Liang, J.D., Han, Y.F., Zhang, J.W., Du, W., Liang, Z.Q. & Li, Z.Z. (2011) Optimal culture conditions for keratinase production by a novel thermophilic Myceliophthora thermophila strain GZUIFR‐H49‐1. Journal of Applied Microbiology 110 (4): 871–880. https://doi.org/10.1111/j.1365-2672.2011.04949.x
  20. Liu, Y.J., Whelen, S. & Hall, B.D. (1999) Phylogenetic relationships among ascomycetes: evidence from an RNA polymerase II subunit. Molecular Biology and Evolution 16 (12): 1799–1808. https://doi.org/10.1093/oxfordjournals.molbev.a026092
  21. López-Rodríguez, L., Burrola-Aguilar, C., Garibay-Orijel, R., Estrada-Zúñiga, M.E., Matías-Ferrer, N. & Argüelles-Moyao, A. (2022) Cordyceps mexicana sp. nov., parasitizing Paradirphia sp. moths: a new sister species of the Cordyceps militaris complex, distributed in central Mexican Quercus-Pinus mixed forests. Mycologia 114 (4): 732–747. https://doi.org/10.1080/00275514.2022.2058854
  22. Mongkolsamrit, S., Noisripoom, W., Thanakitpipattana, D., Wutikhun, T., Spatafora, J.W. & Luangsa-ard, J. (2018) Disentangling cryptic species with Isaria-like morphs in Cordycipitaceae. Mycologia 110: 230–257. https://doi.org/10.1080/00275514.2018.1446651
  23. Mongkolsamrit, S., Noisripoom, W., Tasanathai, K., Khonsanit, A., Thanakitpipattana, D., Himaman, W., Kobmoo, N. & Luangsa-ard, J.J. (2020) Molecular phylogeny and morphology reveal cryptic species in Blackwellomyces and Cordyceps (Cordycipitaceae) from Thailand. Mycological Progress 19 (9): 957–983. https://doi.org/10.1007/s11557-020-01615-2
  24. Peng, X.C., Xiao, Y.P., Zhang, Y., Chomnunti, P., Tangtrakulwanich, K. & Wen, T.C. (2023) Cordyceps poluscapitis sp. nov., an ant-pathogenic fungus from Guizhou, China. Phytotaxa 599 (4): 239–251. https://doi.org/10.11646/phytotaxa.599.4.3
  25. Ronquist, F., Teslenko, M., van der Mark, P., Ayres, D.L., Darling, A., Höhna, S., Larget, B., Liu, L., Suchard, M.A. & Huelsenbeck, J.P. (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology 61: 539–542. https://doi.org/10.1093/sysbio/sys029
  26. Su, C.H. & Wang, H.H. (1986) Phytocordyceps, a new genus of the Clavicipitaceae. Mycotaxon 26, 337–344. [http://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7878780]
  27. Sung, G.H., Hywel-Jones, N.L., Sung, J.M., Luangsa-ard, J.J., Shrestha, B. & Spatafora, J.W. (2007) Phylogenetic classification of Cordyceps and the clavicipitaceous fungi. Studies in Mycology 57: 5–59. https://doi.org/10.3114/sim.2007.57.01
  28. Tamura, K., Stecher, G., Peterson, D., Filipski, A. & Kumar, S. (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution 30: 2725–2729. https://doi.org/10.1093/molbev/mst197
  29. Tasanathai, K., Thanakitpipattana, D., Noisripoom, W., Khonsanit, A., Kumsao, J. & Luangsa-ard, J.J. (2016) Two new Cordyceps species from a community forest in Thailand. Mycological Progress 15: 1–8. https://doi.org/10.1007/s11557–016–1170–3
  30. Trifinopoulos, J., Nguyen, L.T., von Haeseler, A. & Minh, B.Q. (2016) W-IQ-TREE: a fast online phylogenetic tool for maximum likelihood analysis. Nucleic Acids Research 44 (W1): W232–W235. https://doi.org/10.1093/nar/gkw256
  31. Vaidya, G., Lohman, D.J. & Meier, R. (2011) SequenceMatrix: concatenation software for the fast assembly of multi-gene datasets with character set and codon information. Cladistics 27 (2): 171–180. https://doi.org/10.1111/j.1096-0031.2010.00329.x
  32. Wang, Y.B., Wang, Y., Fan, Q., Duan, D.E., Dai, R.Q., Dai, Y.D., Zeng, W.B., Chen, Z.H., Li, D.D., Tang, D.X., Xu, Z.H., Sun, T., Nguyen, T.T., Tran, N.L., Dao, V.M., Zhang, C.M., Huang, L.D., Liu, Y.J., Zhang, X.M., Yang, D.R., Sanjuan, T., Liu, X.Z., Yang, Z.L. & Yu, H. (2020) Multigene phylogeny of the family Cordycipitaceae (Hypocreales): new taxa and the new systematic position of the Chinese cordycipitoid fungus Paecilomyces hepiali. Fungal Diversity 103: 1–46. https://doi.org/10.1007/s13225-020-00457-3
  33. Wang, W.K., Wang, J., Kang, C. & Deng, C.Y. (2022) Species diversity of macrofungi in Doupengshan Provincial Nature Reserve, Guizhou, China. Guizhou Science 40 (3): 1–5. https://doi.org/10.3969/j.issn.1003-6563.2022.03.001
  34. Wang, Y., Dong, Q.Y., Luo, R., Fan, Q., Duan, D.E., Dao, V.M., Wang, Y.B. & Yu, H. (2023) Molecular phylogeny and morphology reveal cryptic species in the Cordyceps militaris complex from Vietnam. Journal of Fungi 9 (6): 1–19. https://doi.org/10.3390/jof9060676
  35. White, T.J., Bruns, T., Lee, S. & Taylor, J. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis, M.A., Gelfand, D.H., Sninsky, J.J. & White, T.J. (Eds.) PCR protocols: a guide to methods and applications. Academic Press, New York, pp. 315–322. https://doi.org/10.1016/B978-0-12-372180-8.50042-1
  36. Yang, Y., Xiao, Y.P., Cheng, Y.N., Xu, F.Y., Han, H.M., Zhang, K. & Luo, X. (2022) Cordyceps yaoluopingensis sp. nov., a new entomopathogenic species from China. Phytotaxa 571 (3): 278–290. https://doi.org/10.11646/phytotaxa.571.3.2
  37. Zha, L.S., Wen, T.C., Huang, S.K., Boonmee, S. & Eungwanichayapant, P.D. (2019) Taxonomy and biology of Cordyceps qingchengensis sp. nov. and its allies. Phytotaxa 416 (1): 14–24. https://doi.org/10.11646/phytotaxa.416.1.2
  38. Zhang, D., Gao, F., Jakovlic, I., Zou, H., Zhang, J., Li, W.X. & Wang, G.T. (2020) PhyloSuite: an integrated and scalable desktop platform for streamlined molecular sequence data management and evolutionary phylogenetics studies. Molecular Ecology Resources 20: 348–355. https://doi.org/10.1111/1755-0998.13096
  39. Zhang, J., Lu, J.J., Zhao, X.C., Kang, C., Wu, Z.J., Deng, C.Y. & Li, Y. (2021) Preliminary report on species diversity of broad-sense Cordyceps in Doupeng Mount area of Guizhou. Edible Fungi Journal 28 (6): 174–187. https://doi.org/10.16488/j.cnki.1005-9873.2021.06.022
  40. Zou, X., Liu, A.Y., Liang, Z.Q., Han, Y.F. & Yang, M. (2010) Hirsutella liboensis, a new entomopathogenic species affecting Cossidae (Lepidoptera) in China. Mycotaxon 111 (1): 39–44. https://doi.org/10.5248/111.39