Abstract
A new wood-inhabiting fungus, Conferticium fissuratum is proposed based on morphological features and molecular phylogenetic evidence. It is characterized by the membranous basidiomata with the tuberculate hymenial surface, a monomitic hyphal system having simple-septate generative hyphae, the presence of the subcylindrical cystidia with the mastoid process and broadly ellipsoid, and the verrucose basidiospores (4.5–6.1 × 3.5–4.7 µm). The phylogenetic tree inferred from ITS sequences revealed that the new species C. fissuratum clusters sister to C. ravum. A full description, illustrations, and results of the phylogenetic analysis of the new species are provided.
References
- Bernicchia, A. & Gorjón, S.P. (2010) Fungi Europaei 12: Corticiaceae s.l. Edizioni Candusso, Lomazzo, pp. 1–1007.
- Cui, B.K., Li, H.J., Ji, X., Zhou, J.L., Song, J., Si, J., Yang, Z.L. & Dai, Y.C. (2019) Species diversity, taxonomy and phylogeny of Polyporaceae (Basidiomycota) in China. Fungal Diversity 97: 137–392. https://doi.org/10.1007/s13225-019-00427-4
- Dai, Y.C. (2011) A revised checklist of corticioid and hydnoid fungi in China for 2010. Mycoscience 52: 69–79. https://doi.org/10.1007/S10267-010-0068-1
- Dai, Y.C., Cui, B.K., Si, J., He, S.H., Hyde, K.D., Yuan, H.S. & Liu, X.Y. (2015) Dynamics of the worldwide number of fungi with emphasis on fungal diversity in China. Mycological Progress 14: 1–9. https://doi.org/10.1007/s11557-015-1084-5
- Dai, Y.C., Yang, Z.L., Cui, B.K., Wu, G., Yuan, H.S., Zhou, L.W., He, S.H., Ge, Z.W., Wu, F., Wei, Y.L., Yuan, Y. & Si, J. (2021) Diversity and systematics of the important macrofungi in Chinese forests. Mycosystema 40: 770–805.
- Dong, J.H., Gu, Z.R., Wang, Y.Y., Shi, Y.M. & Zhao, C.L. (2024a) A new corticioid fungus, Alloexidiopsis sinensis (Auriculariales, Basidiomycota), in China, evidenced by morphological characteristics and phylogenetic analyses. Phytotaxa 658: 227–239. https://doi.org/10.11646/phytotaxa.658.3.1
- Dong, J.H., Li, Q., Yuan, Q., Luo, Y.X., Zhang, X.C., Dai, Y.F., Zhou, Q., Liu, X.F., Deng, Y.L., Zhou, H.M., Muhammad, A. & Zhao, C.L. (2024b) Species diversity, taxonomy, molecular systematics and divergence time of wood-inhabiting fungi in Yunnan-Guizhou Plateau, Asia. Mycosphere 15: 1110–1293. https://doi.org/10.5943/mycosphere/15/1/10
- Duan, Z.Y., Guan, Q.X., Luo, K.Y. & Zhao, C.L. (2023) Morphological and molecular identification of three new resupinate species of Hyphoderma (Hyphodermataceae, Agaricomycetes) from East Asia. Phytotaxa 599: 1–19. https://doi.org/10.11646/phytotaxa.599.1.1
- Felsenstein, J. (1985) Confidence intervals on phylogenetics: an approach using bootstrap. Evolution 39: 783–791. https://doi.org/10.1111/j.1558-5646.1985.tb00420.x
- Hallenberg, N. (1980) New taxa of Corticiaceae from N. Iran (Basidiomycetes). Mycotaxon 11: 447–475
- James, T.Y., Stajich, J.E., Hittinger, C.T. & Rokas, A. (2020) Toward a fully resolved fungal tree of life. Annual Review of Microbiology 74: 291–313. https://doi.org/10.1146/annurev-micro-022020-051835
- Ji, X., Zhou, J.L., Song, C.G., Xu, T.M., Wu, D.M. & Cui, B.K. (2022) Taxonomy, phylogeny and divergence times of Polyporus (Basidiomycota) and related genera. Mycosphere 13: 1–52. https://doi.org/10.5943/mycosphere/13/1/1
- Hu, Y., Karunarathna, S.C., Li, H., Galappaththi, M.C., Zhao, C.L., Kakumyan, P. & Mortimer, P.E. (2022) The impact of drying temperature on basidiospore size. Diversity 14 (4): 239. https://doi.org/10.3390/d14040239
- Katoh, K., Rozewicki, J. & Yamada, K.D. (2019) MAFFT online service: multiple sequence alignment, interactive sequence choice and visualization. Briefings in Bioinformatics 20: 1160–1166. https://doi.org/10.1093/bib/bbx108
- Larsson, E. & Larsson, K.H. (2003) Phylogenetic relationships of russuloid basidiomycetes with emphasis on aphyllophoralean taxa. Mycologia 95: 1037–1065. https://doi.org/10.2307/3761912
- Larsson, A. (2014) AliView: a fast and lightweight alignment viewer and editor for large data sets. Bioinformatics 30: 3276–3278. https://doi.org/10.1093/bioinformatics/btu531
- Liu, S., Shen, L.L., Xu, T.M., Song, C.G., Gao, N., Wu, D.M., Sun, Y.F. & Cui, B.K. (2023) Global diversity, molecular phylogeny and divergence times of the brown-rot fungi within the Polyporales. Mycosphere 14: 1564–1664. https://doi.org/10.5943/mycosphere/14/1/18
- Luo, K.Y. & Zhao, C.L. (2022) A molecular systematics and taxonomy research on Trechispora (Hydnodontaceae, Trechisporales): concentrating on three new Trechispora species from East Asia. Journal of Fungi 8: 1020. https://doi.org/10.3390/jof8101020
- Luo, K.Y. & Zhao, C.L. (2023) A molecular systematics and taxonomy research on Trechispora (Hydnodontaceae, Trechisporales): concentrating on three new Trechispora species from East Asia. Journal of Fungi 8: 1020. https://doi.org/10.3390/jof8101020
- Miller, M.A., Pfeiffer, W. & Schwartz, T. (2012) The CIPRES science gateway. In: Proceedings of the 1st Conference of the Extreme Science and Engineering Discovery Environment: Bridging from the Extreme to the Campus and Beyond. Chicago, IL, USA. 39 pp. https://doi.org/10.1145/2335755.2335836
- Nylander, J.A.A. (2004) MrModeltest v2. Program Distributed by the Author, Evolutionary Biology Centre, Uppsala University: Uppsala, Sweden.
- Petersen, J.H. (1996) Farvekort. In: The Danish Mycological Society’s Colour-Chart. Foreningen til Svampekundskabens Fremme: Greve, Germany, 6 pp.
- Rathnayaka, A.R., Tennakoon, D.S., Jones, G.E., Wanasinghe, D.N., Bhat, D.J., Priyashantha, A.H., Stephenson, S.L., Tibpromma, S. & Karunarathna, S.C. (2024) Significance of precise documentation of hosts and geospatial data of fungal collections, with an emphasis on plant-associated fungi. New Zealand Journal of Botany 62: 1–28. https://doi.org/10.1080/0028825X.2024.2381734
- Ronquist, F. & Huelsenbeck, J.P. (2003) MRBAYES 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19: 1572–1575. https://doi.org/10.1093/bioinformatics/btg180
- Stroheker, S., Weiss, M., Sieber, T.N. & Bugmann, H. (2018) Ecological factors influencing Norway spruce regeneration on nurse logs in a subalpine virgin forest. Forests 9: 120. https://doi.org/10.3390/f9030120
- Su, J.Q., Zhang, X.C., Zhao, C.L. & Zhou, H.M. (2024) Molecular phylogeny and morphology reveal a new wood-inhabiting fungal species, Hyphoderma guangdongense (Polyporales, Basidiomycota), from China. Phytotaxa 661: 75–86. https://doi.org/10.11646/phytotaxa.661.1.5
- Swofford, D.L. (2002) PAUP*: Phylogenetic analysis using parsimony (*and other Methods), Version 4.0b10. Sinauer Associates: Sunderland, MA, USA.
- Tedersoo, L., Bahram, M., Põlme, S., Kõljalg, U., Yorou, N.S., Wijesundera, R., Ruiz, L.V., Vasco-Palacios, A.M., Thu, P.Q., Suija, A., Smith, M.E., Sharp, C., Saluveer, E., Saitta, A., Rosas, M., Riit, T., Ratkowsky, D., Pritsch, K., Põldmaa, K., Piepenbring, M., Phosri, C., Peterson, M., Parts, K., Pärtel, K., Otsing, E., Nouhra, E., Njouonkou, A.L., Nilsson, R.H., Morgado, L.N., Mayor, J., May, T.W., Majuakim, L., Lodge, D.J., Lee, S.S., Larsson, K.H., Kohout, P., Hosaka, K., Hiiesalu, I., Henkel, T.W., Harend, H., Guo, L.D., Greslebin, A., Grelet, G., Geml, J., Gates, G., Dunstan, W., Dunk, C., Drenkhan, R., Dearnaley, J., Kesel, A.D., Dang, T., Chen, X., Buegger, F., Brearley, F.Q., Bonito, G., Anslan, S., Abell, S. & Abarenkov, K. (2014) Global diversity and geography of soil fungi. Science 346: 1256688. https://doi.org/10.1126/science.1256688
- Vasiliauskas, R., Larsson, E., Larsson, K.H. & Stenlid, J. (2005) Persistence and long-term impact of Rotstop biological control agent on mycodiversity in Picea abies stumps. Biological Control 32: 295–304. https://doi.org/10.1016/j.biocontrol.2004.10.008
- Vu, D., Groenewald, M., Vries, M., Gehrmann, T., Stielow, B., Eberhardt, U., Al-Hatmi, A., Groenewald, J.Z., Cardinali, G., Houbraken, J., Boekhout, T., Crous, P., Robert, V. & Verkley, G.J.M. (2019) Large-scale generation and analysis of filamentous fungal DNA barcodes boosts coverage for kingdom fungi and reveals thresholds for fungal species and higher taxon delimitation. Studies in Mycology 92: 135–154. https://doi.org/10.1016/j.simyco.2018.05.001
- Wang, C.G., Zhao, H., Liu, H.G., Zeng, G.Y., Yuan, Y., Dai, Y.C. (2023) A multi‑gene phylogeny clarifies species diversity, taxonomy, and divergence times of Ceriporia and other related genera in Irpicaceae (Polyporales, Basidiomycota). Mycosphere 14 (1): 1665–1729. https://doi.org/10.5943/mycosphere/14/1/19
- Wang, L., Yang, X. & Zhao, C.L. (2024) The wood-decaying fungal diversity unveiled by morphology and phylogeny in Ailaoshan Mountain, Yunnan, China. Phytotaxa 661: 1–30. https://doi.org/10.11646/phytotaxa.661.1.1
- Wang, Y.R., Wu, Y.D., Vlasák, J., Yuan, Y. & Dai, Y.C. (2021) Phylogenetic analysis demonstrating four new species in Megasporoporia sensu lato (Polyporales, Basidiomycota). Mycosphere 12: 1012–1037. https://doi.org/10.5943/mycosphere/12/1/11
- Wei, Y.L., Dai, Y.C. (2004) The ecological function of wood-inhabiting fungi in forest ecosystem. Chinese Journal of Applied Ecology 15: 1935–1938.
- White, T.J., Bruns, T., Lee, S. & Taylor, J. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis, M.A., Gelfand, D.H., Sninsky, J.J. & White, T.J. (eds.) PCR protocols: A guide to methods and applications. Academic Press, San Diego, pp. 315–322. https://doi.org/10.1016/B978-0-12-372180-8.50042-1
- Wijayawardene, N.N., Hyde, K.D., Dai, D.Q., Sánchez-García, M., Goto, B.T., Saxena, R.K., Erdoğdu, M., Selçuk, F., Rajeshkumar, K.C., Aptroot, A., Błaszkowski, J., Boonyuen, N., da Silva, G.A., de Souza, F.A., Dong, W., Ertz, D., Haelewaters, D., Jones, E.B.G., Karunarathna, S.C., Kirk, P.M., Kukwa, M., Kumla, J., Leontyev, D.V., Lumbsch, H.T., Maharachchikumbura, S.S.N., Marguno, F., Martínez-Rodríguez, P., Mešić, A., Monteiro, J.S., Oehl, F., Pawłowska, J., Pem, D., Pfliegler, W.P., Phillips, A.J.L., Pošta, A., He, M.Q., Li, J.X., Raza, M., Sruthi, O.P., Suetrong, S., Suwannarach, N., Tedersoo, L., Thiyagaraja, V., Tibpromma, S., Tkalčec, Z., Tokarev, Y.S., Wanasinghe, D.N., Wijesundara, D.S.A., Wimalaseana, S.D.M.K., Madrid, H., Zhang, G.Q., Gao, Y., Sánchez-Castro, I., Tang, L.Z., Stadler, M., Yurkov, A. & Thines, M. (2022) Outline of Fungi and fungus like taxa-2021. Mycosphere 13: 53–453. https://doi.org/10.5943/mycosphere/13/1/2
- Wu, F., Man, X.W., Tohtirjap, A. & Dai, Y.C. (2022) A comparison of polypore funga and species composition in forest ecosystems of China, North America, and Europe. Forest Ecosystems 9: 100051. https://doi.org/10.1016/j.fecs.2022.100051
- Wu, S.H. (1996) Studies on Gloeocystidiellum sensu lato (Basidiomycotina) in Taiwan. Mycotaxon. 58: 1–68
- Yang, Y., Li, R., Jiang, Q.Q., Zhou, H.M., Muhammad, A., Wang, H.J. & Zhao, C.L. (2024) Phylogenetic and taxonomic analyses reveal three new wood-inhabiting fungi (Polyporales, Basidiomycota) in China. Journal of Fungi 10: 55. https://doi.org/10.3390/jof10010055
- Yuan, Y., Chen, J.J., Korhonen, K., Martin, F. & Dai, Y.C. (2021) An updated global species diversity and phylogeny in the forest pathogenic genus Heterobasidion (Basidiomycota, Russulales). Frontiers in Microbiology 11: 596393. https://doi.org/10.3389/fmicb.2020.596393
- Zhang, X.C., Li, Y.C., Wang, Y.Y., Xu, Y., Zhao, C.L. & Zhou, H.M. (2024) Xylodon asiaticus (Hymenochaetales, Basidiomycota), a new species of corticioid fungus from southern China. Phytotaxa 634: 1–15. https://doi.org/10.11646/phytotaxa.634.1.1
- Zhao, H., Wu, Y.D., Yang, Z.R., Liu, H.G., Wu, F., Dai, Y.C. & Yuan, Y. (2024) Polypore funga and species diversity in tropical forest ecosystems of Africa, America and Asia, and a comparison with temperate and boreal regions of the Northern Hemisphere. Forest Ecosystem 100: 100200. https://doi.org/10.1016/j.fecs.2024.100200
- Zhao, C.L. & Wu, Z.Q. (2017) Ceriporiopsis kunmingensis sp. nov. (Polyporales, Basidiomycota) evidenced by morphological characters and phylogenetic analysis. Mycological Progress 16: 93–100. https://doi.org/10.1007/s11557-016-1259-8
- Zhao, Y.L. & Zhao, C.L. (2023) A corticioid fungus, Gloeocystidiellum yunnanense sp. nov. (Russulales) with characteristic gloeocystidia from southern China. Nova Hedwigia 116: 155–170. https://doi.org/10.1127/nova_hedwigia/2023/0725
- Zhao, C.L., Qu, M.H., Huang, R.X. & Karunarathna, S. (2023) Multi-gene phylogeny and taxonomy of the wood-rotting fungal genus Phlebia sensu lato (Polyporales, Basidiomycota). Journal of Fungi 9: 320. https://doi.org/10.3390/jof9030320
- Zhao, H., Wu, Y.D., Yang, Z.R., Liu, H.G., Wu, F., Dai, Y.C. & Yuan, Y. (2024) Polypore and a comparison with temperate and boreal regions of the Northern Hemisphere. Forest Ecosystem 11: 100200. https://doi.org/10.1016/j.fecs.2024.100200
- Zhou, H.M., Gu, Z.R. & Zhao, C.L. (2024) Molecular phylogeny and morphology reveal a new species of Asterostroma from Guizhou Province, China. Phytotaxa 635: 173–181. https://doi.org/10.11646/phytotaxa.635.2.7
- Zhou, H.M., Dai, Y.C., Bian, L.S., Liu, H.G., Vlasák, J. & Yuan, Y. (2024) Diversity, divergence time, and biogeography of the genus Albatrellus (Agaricomycetes, Russulales). Mycology. [39 pp.] https://doi.org/10.1080/21501203.2024.2386021