Abstract
A new species of Amanita, A. minqingensis, is described from Fujian province, China, based on morphological and multi-phylogenetic sequencing including the nuclear ribosomal large subunit (nrLSU), nuclear ribosomal internal transcribed spacer (ITS), RNA polymerase large subunit-2 (rpb2), and translation elongation factor-α (tef1-α) loci. We identify the species as a member in Amanita section Vaginatae, subg. Amanita. The new species is characterized by its tan pileus with putty verrucose volval remnants, the absence of clamps and globose to sub-globose basidiospores ((9‒)9.2‒10.3‒11 × (8.5‒)9‒9.5‒10.5). Detailed descriptions, color photographs of fresh basidiomata, line drawing of microscopic characters and a phylogenetic tree based on ITS sequence data are are provided.
References
- Bas, C. (1969) Morphology and subdivision of Amanita and a monograph of its section Lepidella. Persoonia 5 (4): 285–579.
- Cai, Q., Cui, Y.Y. & Yang, Z.L. (2016) Lethal Amanita species in China. Mycologia 108 (5): 993–1009. https://doi.org/10.3852/16-008
- Cai, Q., Tulloss, R.E., Tang, L.P., Tolgor, B., Zhang, P., Chen, Z.H. & Yang, Z.L. (2014) Multi-locus phylogeny of lethal amanitas: implications for species diversity and historical biogeography. BMC Evolutionary Biology 14 (1): 143. https://doi.org/10.1186/1471-2148-14-143
- Corner, E.J.H. & Bas, C. (1962) The genus Amanita in Singapore and Malaya. Persoonia 2: 241–304.
- Crous, P.W., Luangsa-Ard, J.J., Wingfield, M.J., Carnegie, A.J., Hernández-Restrepo, M., Lombard, L., Roux, J., Barreto, R.W., Baseia, I.G., Cano-Lira, J.F., Martín, M.P., Morozova, O.V., Stchigel, A.M., Summerell, B.A., Brandrud, T.E., Dima, B., García, D., Giraldo, A., Guarro, J., Gusmão, L.F.P., Khamsuntorn, P., Noordeloos, M.E., Nuankaew, S., Pinruan, U., Rodríguez-Andrade, E., Souza-Motta, C.M., Thangavel, R., van Iperen, A.L., Abreu, V.P., Accioly, T., Alves, J.L., Andrade, J.P., Bahram, M., Baral, H.O., Barbier, E., Barnes, C.W., Bendiksen, E., Bernard, E., Bezerra, J.D.P., Bezerra, J.L., Bizio, E., Blair, J.E., Bulyonkova, T.M., Cabral, T.S., Caiafa, M.V., Cantillo, T., Colmán, A.A., Conceição, L.B., Cruz, S., Cunha, A.O.B., Darveaux, B.A., da Silva, A.L., da Silva, G.A., da Silva, G.M., da Silva, R.M.F., de Oliveira, R.J.V., Oliveira, R.L., De Souza, J.T., Dueñas, M., Evans, H.C., Epifani, F., Felipe, M.T.C., Fernández-López, J., Ferreira, B.W., Figueiredo, C.N., Filippova, N.V., Flores, J.A., Gené, J., Ghorbani, G., Gibertoni, T.B., Glushakova, A.M., Healy, R., Huhndorf, S.M., Iturrieta-González, I., Javan-Nikkhah, M., Juciano, R.F., Jurjević, Ž., Kachalkin, A.V., Keochanpheng, K., Krisai-Greilhuber, I., Li, Y.C., Lima, A.A., Machado, A.R., Madrid, H., Magalhães, O.M.C., Marbach, P.A.S., Melanda, G.C.S., Miller, A.N., Mongkolsamrit, S., Nascimento, R.P., Oliveira, T.G.L., Ordoñez, M.E., Orzes, R., Palma, M.A., Pearce, C.J., Pereira, O.L., Perrone, G., Peterson, S.W., Pham, T.H.G., Piontelli, E., Pordel, A., Quijada, L., Raja, H.A., Rosas de Paz, E., Ryvarden, L., Saitta, A., Salcedo, S.S., Sandoval-Denis, M., Santos, T.A.B., Seifert, K.A., Silva, B.D.B., Smith, M.E., Soares, A.M., Sommai, S., Sousa, J.O., Suetrong, S., Susca, A., Tedersoo, L., Telleria, M.T., Thanakitpipattana, D., Valenzuela-Lopez, N., Visagie, C.M., Zapata, M. & Groenewald, J.Z. (2018) Fungal Planet description sheets: 785-867. Persoonia 41: 238–417. https://doi.org/ 10.3767/persoonia.2018.41.12
- Cui, Y.Y., Cai, Q., Tang, L.P., Liu, J.W. & Yang, Z.L. (2018) The family Amanitaceae: molecular phylogeny, higher-rank taxonomy and the species in China. Fungal Diversity 91: 5–230. https://doi.org/10.1007/s13225-018-0405-9
- Cui, Y.Y., Hao, Y.J., Guo, T., Yang, Z.L. & Cai, Q. (2023) Species diversity of Amanita section Vaginatae in Eastern China, with a description of four new species. Journal of Fungi 9 (8): 862. https://doi.org/10.3390/jof9080862
- Gardes, M. & Bruns, T.D. (1993) ITS primers with enhanced specificity for basidiomycetes-application to the identification of mycorrhizae and rusts. Molecular Ecology 2 (2): 113–118. https://doi.org/10.1111/j.1365-294x.1993.tb00005.x
- Kibby, G. (2012) The genus Amanita in Great Britain. Privately published.
- Kornerup, A. & Wanscher, J.H. (1981) Taschenlexikon der Farben, 3rd edn. Muster-Schmidt Verlag, Zürich und Göttingen.
- Kumar, S., Stecher, G. & Tamura, K. (2016) MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular Biology and Evolution 33 (7): 1870–1874. https://doi.org/10.1093/molbev/msw054
- Liu, Y.S., Liu, J.K., Mortimer, P.E. & Lumyong, S.L. (2021) Amanita submelleialba sp. nov. in section Amanita from northern Thailand. Phytotaxa 513 (2): 129–140. https://doi.org/10.11646/phytotaxa.513.2.4
- Mighell, K.S., Henkel, T.W., Koch, R.A., Chin, M.L., Brann, M.A. & Aime, M.C. (2021) Amanita in the Guineo-Congolian rainforest: Epitypes and new species from the Dja Biosphere Reserve, Cameroon. Mycologia 113 (1): 168–190. https://doi.org/10.1080/00275514.2020.1816386
- Niskanen, T., Lücking, R., Dahlberg, A., Gaya, E., Suz, L.M., Mikryukov, V., Liimatainen, K., Druzhinina, I., Westrip, J.R.S., Mueller, G.M., Martins-Cunha, K., Kirk, P., Tedersoo, L. & Antonelli, A. (2023) Pushing the frontiers of biodiversity research: Unveiling the global diversity, distribution, and conservation of fungi. Annual Review of Environment and the Resources 48: 149–176. https://doi.org/10.1146/annurev-environ-112621-090937
- Okhovat, A., Cruces, W., Docampo-Palacios, M.L., Ray, K.P. & Ramirez, G.A. (2023) Psychoactive isoxazoles, muscimol, and isoxazole derivatives from the Amanita (Agaricomycetes) species: Review of new trends in synthesis, dosage, and biological properties. International Journal of Medicinal Mushrooms 25 (9): 1–10. https://doi.org/10.1615/IntJMedMushrooms.2023049458
- Rehner, S.A. & Buckley, E. (2005) A Beauveria phylogeny inferred from nuclear ITS and EF1-a sequences: evidence for cryptic diversification and links to Cordyceps teleomorphs. Mycologia 97 (1): 84–98. https://doi.org/10.3852/mycologia.97.1.84
- Ronquist, F., Teslenko, M., van der Mark, P., Ayres, D.L., Darling, A., Höhna, S., Larget, B., Liu, L., Suchard, M.A. & Huelsenbeck, J.P. (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology 61 (3): 539–542. https://doi.org/10.1093/sysbio/sys029
- Stamatakis, A. (2014) RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 30: 1312–1313. https://doi.org/10.1093/bioinformatics/btu033
- Tompson, J.D., Gibson, T.J., Plewnlak, F., Jianmougin, F. & Higgins, D.G. (1997) The Clustal X windows interfaces: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research 24: 4876–4882. https://doi.org/10.1093/nar/25.24.4876
- Vilgalys, R. & Hester, M. (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. Journal of Bacteriology 172 (8): 4238–4246. https://doi.org/10.1128/jb.172.8.4238-4246.1990
- White, T.J., Bruns, T.D., Lee, S.B. & Taylor, J.W. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis, M.A., Gelfand, D.H., Sninsky, J.J. & White, T.J. (Eds.) PCR protocols: A guide to methods and applications 18. Academic Press, pp. 315–322. https://doi.org/10.1016/B978-0-12-‒3721808.50042-1
- Yang, Z.L. (1997) Die Amanita-Arten von Südwestchina. Bibliotheca Mycologica 170: 1–240.
- Yang, Z.L. (2004) Two new species of Amanita (Basidiomycota) from China. In: Agerer, R., Piepenbring, M. & Blanz, P. (Eds.) Frontiers Basidiomycete Mycology. IHW Verlag, Eching, pp. 315–324.
- Yang, Z.L. (2015) Atlas of the Chinese species of Amanitaceae. Science Press, Beijing.
- Zhang, P., Yang, Z.L. & Ge, Z.W. (2005) Two new species of Ramaria from southwestern China. Mycotaxon 94: 235–240. https://doi.org/10.1007/s11046-005-6871-0
- Zhou, H., Guo, M., Zhuo, L., Yan, H., Sui, X., Gao, Y. & Hou, C. (2023) Diversity and taxonomy of the genus Amanita (Amanitaceae, Agaricales) in the Yanshan Mountains, Northern China. Frontiers in Plant Science 14: 1226794. https://doi.org/10.3389/fpls.2023.1226794