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Issue: Vol. 653 No. 1: 13 Jun. 2024
Type: Article
Published: 2024-06-13
DOI: 10.11646/phytotaxa.653.1.4
Page range: 54-66
Abstract views: 62
PDF downloaded: 1

A new omphalinoid species, Entoloma khushabense (Entolomataceae, Basidiomycota) from Punjab, Pakistan

Fungal Biology and Systematics Research Laboratory, Institute of Botany, University of the Punjab, Quaid-e-Azam Campus, Lahore 54590, Pakistan
Fungal Biology and Systematics Research Laboratory, Institute of Botany, University of the Punjab, Quaid-e-Azam Campus, Lahore 54590, Pakistan
Fungal Biology and Systematics Research Laboratory, Institute of Botany, University of the Punjab, Quaid-e-Azam Campus, Lahore 54590, Pakistan
caulocystidia evolution Khushab Noorpur Thal nrDNA Fungi

Abstract

A new species, Entoloma khushabense, is described and illustrated here based on morphological and molecular evidence from Pakistan. The key features of the new species that make it distinct from closely related taxa include the presence of conspicuous cheilocystidia and caulocystidia, and hyphae having constrictions in the walls. Morphological data and phylogenetic analyses based on internal transcribed spacer (ITS) and large sub-unit (LSU) regions of nuclear rDNA support that the present species differs from previously known members of Entoloma.

References

  1. Ahmad, S. (1962) Further contributions to the fungi of Pakistan. II. Biologia 8: 123‒150.
  2. Ahmad, S., Iqbal, S.H. & Khalid, A.N. (1997) Fungi of Pakistan. Sultan Ahmad Mycological Society of Pakistan, Lahore.
  3. Altschul, S.F., Gish, W., Miller, W., Myers, E.W. & Lipman, D.J. (1990) Basic local alignment search tool. Journal of Molecular Biology 215 (3): 403–410.
  4. Aman, N., Khalid, A.N. & Moncalvo, J-M. (2022) A compendium of macrofungi of Pakistan by ecoregions. MycoKeys 89: 171–233. https://doi.org/10.3897/mycokeys.89.81148
  5. Baroni, T.J. & Halling, R.E. (2000) Some Entolomataceae (Agaricales) from Costa Rica. Brittonia 52: 121–135. https://doi.org/10.2307/2666502.
  6. Baroni, T.J. & Matheny, P.D. (2011) A re-evaluation of gasteroid and cyphelloid species of Entolomataceae from Eastern North America. Harvard Papers in Botany 16: 293–310. https://doi.org/10.3100/0.25.016.0205
  7. Battistin, E., Deng, W.Q., Li, T.H. & Gelardi, M. (2014) A new species of Entoloma s. l. (Agaricales) from Nan’ao Island, south-eastern China. Sydowia 66 (2): 257–264. https://doi.org/10.12905/0380.sydowia66(2)2014-0257
  8. Bentham, G. (1844) Notes on Mimoseae, with a synopsis of species. The London Journal of Botany 3: 82–112. [https://www.biodiversitylibrary.org/item/6314#page/86/mode/1up]
  9. Brandrud, T.E., Bendiksen, E., Jordal, J.B., Weholt, Dima, B., Morozova, O. & Noordeloos, M.E. (2020) On some Entoloma species (Tricholomatinae, Basidiomycota) little known or new to Norway. Agarica 9: 31–52.
  10. Chaudhari, S.K., Arshad, M., Mustafa, G., Fatima, S., Amjad, M.S. & Yasmeen, F. (2014) Foliar epidermal anatomy of grasses from Thal desert, district Khushab, Pakistan. International Journal of Biosciences 4 (8): 62–70. https://doi.org/10.12692/ijb/4.8.62-70
  11. Co-David, D., Langeveld, D. & Noordeloos, M.E. (2009) Molecular phylogeny and spore evolution of Entolomataceae. Persoonia 23: 147–176. https://doi.org/10.3767/003158509X480944
  12. De Jussieu, A. (1830) Mémoire sur la famille de Myrsinacées. Memoirs Museum d’Histoire Naturelle19: 153–304.
  13. Dehnhardt, F. (1832) Catalogus Plantarum Horti Camaldulensis. Editio Secunda; Ortobotanico: Napoli, Italy. pp. 1–40. Available online: http://www.ortobotanico.unina.it/Catalogo_P/H_camaldulensis/Catalogo.htm (accessed 12 June 2024)
  14. Dima, B., Brandrud, T.E., Corriol, G., Jansen, G.M., Jordal, J.B., Khalid, A.N., Larsson, E., Lorås, J., Morozova, O.V., Naseer, A., Noordeloos, M.E., Rossi, W., Santamaria, S., Sarwar, S., Sesli, E., Usman, M., Afshan, N.S., Ahmad, I., Banerjee, A., Banerjee, K., Bendiksen, E., Rodrigues Da Silva Colombo, D., De Kesel, A., Dovana, F., Ferisin, G., Hussain, S., Islam, S., Jesus, A.L., Kaygusuz, O., Krisai-Greilhuber, I., Mahammad, S., Mishra, D.K., Nath, P.S., De Oliveira Da Paixã, S.C., Panja, B., Papp, V., Amorim Pires-Zottarelli, C.L., Radnóti, A., Rana, D., Saha, R., Türkekul, I. & Haelewaters, D. (2021) Fungal Systematics and Evolution: FUSE 7. Sydowia 73: 271–340. https://doi.org/10.12905/0380.sydowia73-2021-0271
  15. Edgar, R.C. (2004) MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research 32: 1792–1797. https://doi.org/10.1093/nar/gkh340
  16. Fatima, N., Usman, M. & Khalid, A.N. (2023) Entoloma punjabense sp. nov. of Rusticoides group from Chichawatni Forest, Pakistan. Phytotaxa 612 (2): 217–228. https://doi.org/10.11646/phytotaxa.612.2.6
  17. Fries, E.M. (1838) Epicrisis Systematis Mycologici. Typographia Academica, Uppsala, 610 pp.
  18. Gardes, M. & Bruns, T.D. (1993) ITS primers with enhanced specificity for basidiomycetes‐application to the identification of mycorrhizae and rusts. Molecular ecology 2 (2): 113–118. https://doi.org/10.1111/j.1365-294X.1993.tb00005.x
  19. Gates, G.M. & Noordeloos, M.E. (2007) Preliminary studies in the genus Entoloma in Tasmania – I. Persoonia 19: 157–226.
  20. Gillet, C.C. (1876) Les Hyménomycètes ou Description de tous les Champignons qui Croissent en France. Ch. Thomas, pp. 177–560
  21. Haelewaters, D., Dima, B., Abdel-Hafiz, A.I., Abdel-Wahab, M.A., Abul-Ezz, S.R., Acar, I., Aguirre-Acosta, E., Aime, M.C., Aldemir, S., Ali, M., Ayala-Vásquez, O., Bakhit, M.S., Bashir, H., Battistin, E., Bendiksen, E., Castro-Rivera, R., Çolak, Ö.F., De Kesel, A., de la Fuente, J.I., Dizkýrýcý, A., Hussain, S., Jansen, G.M., Kaygusuz, O., Khalid, A.N., Khan, J., Kiyashko, A.A., Larsson, E., Martínez-González, C.R., Morozova, O.V., Niazi, A.R., Noordeloos, M.E., Pham, T.H.G., Popov, E.S., Psurtseva, N.V., Schoutteten, N., She,H., Türkekul, Ý., Verbeken, A., Ahmad, H., Afshan, N.S., Christe, P., Fiaz, M., Glaizot, O., Liu, J., Majeed, J., Markotter, W., Nagy, A., Nawaz, H., Papp, V., Péter, Á., Pfliegler, W.P., Qasim, T., Riaz, M., Sándor, A.D., Szentiványi, T., Voglmayr, H., Yousaf, N. & Krisai-Greilhuber, I. (2020) Fungal systematics and evolution 6. Sydowia 72: 231–356. https://doi.org/10.12905/0380.sydowia72-2020-0271
  22. Hall, T.A. (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 41: 95–98. https://doi.org/10.1073/pnas.1117018109
  23. He, X.L., Horak, E., Wang, D., Li, T.H., Peng, W.H. & Gan, B.C. (2019) Descriptions of five new species in Entoloma subgenus Claudopus from China, with molecular phylogeny of Entoloma sl. MycoKeys 61: 1–26. https://doi.org/10.3897/mycokeys.61.46446
  24. He, X.L., Li, T.H., Xi, P.G., Jiang, Z.D. & Shen, Y.H. (2013) Phylogeny of Entoloma sl subgenus Pouzarella, with descriptions of five new species from China. Fungal Diversity 58: 227–243. https://doi.org/10.1007/s13225-012-0212-7.
  25. Henkel, T.W., Aime, M.C., Largent, D.L. & Baroni, T.J. (2010) The Entolomataceae of the Pakaraima Mountains of Guyana 5: new species of Alboleptonia. Mycotaxon 114: 115–126. http://dx.doi.org/10.5248/114.115
  26. Horak, E. (1980) Entoloma (Agaricales) in Indomalaya and Australasia. Beihefte Nova Hedwigia 65: 1–352.
  27. Horak, E. (2008) The fungi of New Zealand, vol. 5: Agaricales of New Zealand 1: Pluteaceae – Entolomataceae. Fungal Diversity Press, Hong Kong. 305 pp.
  28. Izhar, A., Qasim, T., Asif, M., Niazi, A.R. & Khalid, A.N. (2023) Entoloma griseofibrillosus (Entolomataceae, Basidiomycota), a new species from Punjab, Pakistan. Phytotaxa 589 (1): 1–14. https://doi.org/10.11646/phytotaxa.589.1.1
  29. Izhar, A., Usman, M., Kiran, M. & Khalid, A.N. (2022) Two New species of Entoloma (Agaricales, Basidiomycota) from Khyber Pakhtunkhwa, Pakistan. Phytotaxa 574: 226–240. https://doi.org/10.11646/phytotaxa.574.3.2
  30. Karstedt, F. & Capelari, M. (2015) A new species of Entolomataceae with cuboidal basidiospores from the São Paulo Metropolitan Region, Brazil. Mycosphere 6: 69–73. https://doi.org/10.5943/mycosphere/6/1/8
  31. Karstedt, F. & Capelari, M. (2017) A new species of Entoloma subgenus Trichopilus from Atlantic Forest Region (Brazil). Sydowia 69: 23–28. https://doi.org/10.12905/0380.sydowia69-2017-0023
  32. Karstedt, F. (2007) A new combination and new records of Pouzarella (Agaricales, Entolomataceae) from Brazil. Mycotaxon 102: 147–154.
  33. Karstedt, F., Capelari, M., Baroni, T.J., Largent, D.L. & Bergemann, S.E. (2019) Phylogenetic and morphological analyses of species of the Entolomataceae (Agaricales, Basidiomycota) with cuboid basidiospores. Phytotaxa 391 (1): 1–27. https://doi.org/10.11646/phytotaxa.391.1.1
  34. Karsten, G.K.W.H. (1880–1883) Deutsche Flora: Pharmaceutisch-medicinische Botanik. Ein Grundriss der systematischen Botanik zum Selbststudium für Ärzte, Apotheker und Botaniker. Mit Abbildungen von 1138 Pflanzenarten. J. M. Späth Verlag, Berlin. pp. 1–1284.
  35. Khalid, A.N. (2022) A Checklist of Macrofungi of Pakistan published from 1998–2020. Pakistan Journal of Botany 54: 1947–1962. https://doi.org/10.30848/PJB2022-5(23)
  36. Khan, Z., Arshad, M.A., Niazi, A.R. & Khalid, A.N. (2023b) A new species of Parasola section Conopileae (Psathyrellaceae) from Pakistan. Turkish Journal of Botany 47 (6): 606–619. https://doi.org/10.55730/1300-008X.2788
  37. Khan, Z., Izhar, A. & Khalid, A.N. (2023a) Leucoagaricus thallensis (Agaricaceae; Basidiomycota), a new species from Punjab, Pakistan. Phytotaxa 594 (3): 163–177. https://doi.org/10.11646/phytotaxa.594.3.1
  38. Kinoshita, A., Sasaki, H. & Nara, K. (2012) Multiple origins of sequestrate basidiomes within Entoloma inferred from molecular phylogenetic analyses. Fungal Biology 116: 1250–1262. https://doi.org/10.1016/j.funbio.2012.09.006
  39. Kluting, K.L., Baroni, T.J. & Bergemann, S.E. (2014) Toward a stable classification of genera within the Entolomataceae: a phylogenetic re-evaluation of the Rhodocybe-Clitopilus clade. Mycologia 106 (6): 1127–1142. https://doi.org/10.3852/13-270
  40. Kummer, P. (1871) Der Führer in die Pilzkunde. C. Luppe, Zerbst, 146 pp.
  41. Mabberley, D.J. (2008) Mabberley’s Plant-book: A Portable Dictionary of Plants, Their Classifications, and Uses. Cambridge University. pp. 77–476.
  42. Manimohan, P., Joseph, A.V. & Leelavathy, K.M. (1995) The genus Entoloma in Kerala State, India. Mycological Research 99 (9): 1083–1097.
  43. Manimohan, P., Noordeloos, M.E. & Dhanya, A.M. (2006) Studies on the genus Entoloma (Basidiomycetes, Agaricales) in Kerala State, India. Persoonia 19: 45–93.
  44. Miller, M.A., Pfeiffer, W. & Schwartz, T. (2010) Creating the CIPRES Science Gateway for inference of large phylogenetic trees. In: Proceedings of the Gateway computing environments workshop (GCE). New Orleans, Louisiana, pp. 1–8. https://doi.org/10.1109/GCE.2010.5676129.
  45. Morgado, L.N., Noordeloos, ME., Lamoureux, Y. & Geml, J. (2013) Multi-gene phylogenetic analyses reveal species limits, phylogeographic patterns, and evolutionary histories of key morphological traits in Entoloma (Agaricales, Basidiomycota). Persoonia-Molecular Phylogeny and Evolution of Fungi 31: 159–178. https://doi.org/10.3767/003158513X673521
  46. Morozova, O.V., Noordeloos, M.E. & Vila, J. (2014) Entoloma subgenus Leptonia in boreal-temperate Eurasia: towards a phylogenetic species concept. Persoonia-Molecular Phylogeny and Evolution of Fungi 32 (1): 141–169. https://doi.org/10.3767/003158514X681774
  47. Munsell, A.H. (1994) Munsell Soil Color Charts. Macbeth: Newburgh, NY, USA, 37 pp.
  48. Noordeloos, M.E. & Gates, G.M. (2012) The Entolomataceae of Tasmania. Fungal diversity research series, vol. 22. Springer, London.
  49. Noordeloos, M.E. (1981) Introduction to the taxonomy of the genus Entoloma sensu lato (Agaricales). Persoonia 11: 121–151.
  50. Noordeloos, M.E. (1984) Studies in Entoloma 10–13. Persoonia 12: 195–223.
  51. Noordeloos, M.E. (1985) Notulae ad Floram agaricinam neerlandicam—X-XI. Entoloma. Persoonia 12: 457–462.
  52. Noordeloos, M.E. (1992) Entoloma s.l. In: Candusso, M. (ed.) Funghi Europaei, vol 5. Giovanna Biella, Saronno, pp. 1–760.
  53. Noordeloos, M.E. (2004) Entoloma s.l. In: Fungi Europaei 5A. Edizioni Candusso, Alassio. 618 pp.
  54. Noordeloos, M.E., Lorås, J.A., Eidissen, S.E., Brandrud, T.E., Bendiksen, E., Morozova, O., Jordal, J.B., Weholt, Ø., Jansen, G.M., Larsson, E. & Bálint, D. (2021) Three new Entoloma species of the Cyanula clade from (sub)alpine habitats in Northern Norway and Sweden. Sydowia 73: 185–196. https://doi.org/10.12905/0380.sydowia73-2020-0185
  55. Noordeloos, M.E., Vila, J., Jordal, J.B., Kehlet, T., Brandrud, T.E., Bendiksen, E., Moreau, P.A., Dondl, M., Lorås, J. & Dima, B. (2022) Contributions to the revision of the genus Entoloma (Basidiomycota, Agaricales) in Europe: six new species from subgenus Cyanula and typification of E. incarnatofuscescens. Fungal Systematics and Evolution 9: 87–97. https://doi.org/10.3114/fuse.2022.09.06
  56. Noordeloos, M.E., Weholt, Ø., Bendiksen, E., Brandrud, T.E., Eidissen, S.E., Lorås, J., Morozova, O. & Dima, B. (2018) Entoloma aurorae-borealis sp. nov. and three rare Entoloma species in the Sinuatum clade (subg. Entoloma) from northern Europe. Sydowia 70: 199–210. https://doi.org/10.12905/0380.sydowia70-2018-0199
  57. Osmundson, T.W., Robert, V.A., Schoch, C.L., Baker, L.J., Smith, A., Robich, G., Mizzan, L. & Garbelotto, M.M. (2013) Filling gaps in biodiversity knowledge for macrofungi: contributions and assessment of an herbarium collection DNA barcode sequencing project. PloS ONE 8: e62419. https://doi.org/10.1371/journal.pone.0062419
  58. Pacioni, G. (1987) I funghi delle aree bruciate nel bacino del Mediterraneo. Micologia e Vegetazione Mediterranea 2: 67–148.
  59. Pegler, D.N. (1977) Entolomataceae (Agaricales) from India & Sri Lanka. Kew Bull 32: 189–220.
  60. Reschke, K., Noordeloos, M.E., Manz, C., Hofmann, T.A., Rodríguez-Cedeño, J., Dima, B. & Pipenbring, M. (2022) Fungal diversity in the tropics: Entoloma spp. in Panama. Mycological Progress 21: 93–145. https://doi.org/10.1007/s11557-021-01752-2
  61. Roxburg, W. (1814) Hortus Bengalensis or a Catalogue of the Plants Growing in the Honourable East India Company’s Botanic Garden at Calcutta. pp. 1–300.
  62. Shaheen, H., Qureshi, R., Qaseem, M.F., Amjad, M.S. & Bruschi, P. (2017) The cultural importance of indices: A comparative analysis based on the useful wild plants of Noorpur Thal Punjab, Pakistan. European Journal of Integrative Medicine 12: 27–34. http://dx.doi.org/10.1016/j.eujim.2017.04.003
  63. Singer, R. (1986) The Agaricales in modern taxonomy, 4th edition. Koeltz Scientific Books, Koenigstein.
  64. Smith, M.E., Amses, K.R., Elliott, T.F., Obase, K., Aime, M.C. & Henkel, T.W. (2015) New sequestrate fungi from Guyana: Jimtrappea guyanensis gen. sp. nov., Castellanea pakaraimophila gen. sp. nov., and Costatisporus cyanescens gen. sp. nov. (Boletaceae, Boletales). IMA fungus 6 (2): 297– 317. https://doi.org/10.5598/imafungus.2015.06.02.03
  65. Sultana, K., Rauf, C.A., Riaz, A., Naz, F., Irshad, G. & Haque, M.I. (2011) Checklist of Agarics of Kaghan Valley-1. Pakistan Journal of Botany 43: 1777–1787.
  66. Sulzbacher, M.A., Orihara, T., Grebenc, T., Wartchow, F., Smith, M.E., Martín, M.P., Giachini, A.J. & Baseia, I.G. (2020) Longistriata flava (Boletaceae, Basidiomycota) – a new monotypic sequestrate genus and species from Brazilian Atlantic Forest. MycoKeys 62: 53–73. https://doi.org/10.3897/mycokeys.62.39699
  67. Varga, T., Krizsán, K., Földi, C., Dima, B., Sánchez-García, M., Sánchez-Ramírez, S., Szöllősi, G.J., Szarkándi, J.G., Papp, V., Albert, L. & Andreopoulos, W. (2019) Megaphylogeny resolves global patterns of mushroom evolution. Nature Ecology & Evolution 3 (4): 668–678. https://doi.org/10.1038/s41559-019-0834-1
  68. Vellinga, E.C. (2001) Agaricaceae. In: Noordeloos, M.E., Kuyper, T.W. & Vellinga, E.C. (eds.) Flora Agaricina Neerlandica 5. Rotterdam: A.A. Balkema Publishers, 220 pp.
  69. Vila, J., Caballero, F., i Pericay, J.C., Alvarado, P., Català, S., Higelmo, M.A. & Llimona, X. (2014) Preliminary morphologic and molecular study of the Entoloma rusticoides group (Agaricales-Basidiomycota). Revista Catalana de Micologia 35: 65–99.
  70. Vilgalys, R. & Hester, M. (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. Journal of Bacteriology 172: 4238–4246. https://doi.org/10.1128/jb.172.8.4238-4246.1990
  71. Vizzini, A., Musumeci, E., Ercole, E. & Contu, M. (2011) Clitopilus chrischonensis sp. nov. (Agaricales, Entolomataceae), a striking new fungal species from Switzerland. Nova Hedwigia 92: 425–434. https://doi.org/10.1127/0029-5035/2011/0092-0425
  72. Wang, J.R. & Bau, T. (2013) A new species and a new record of the genus Entoloma from China. Mycotaxon 124: 165–171. http://dx.doi.org/10.5248/124.165
  73. White, T.J., Bruns, T., Lee, S. & Taylor, J. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenies. In: Innis, M.A., Gelfand, D.H., Sninsky, J.J., White, T.J. (eds.) PCR protocols: a guide to methods and applications. San Diego: Academic Press. pp. 315–322. https://doi.org/10.1016/B978-0-12-372180-8.50042-1
  74. Wight, R. & Arnott, G.A.W. (1834) Prodromus Florae Peninsulae Indiae Orientalis 1. Parbury, Allen & Co., London, pp. 1–480. https://doi.org/10.5962/bhl.title.252
  75. Yousaf, Z. (2018) First Report of ethnobotanical studies of tehsil Noorpur Thal, District Khushab, Punjab, Pakistan. International Journal of Environment, Agriculture and Biotechnology 3 (4): 264399. http://dx.doi.org/10.22161/ijeab/3.4.15
  76. Zhao, R.L., Karunarathna, S.C., Raspe, O., Parra, L.A., Guinberteau, J., Moinard, M., Kesel, A.D., Barroso, R., Courtecuisse, R., Hyde, K.D., Guelly, A.K., Desjardin, D.E. & Callac, P. (2011) Major clades in tropical Agaricus. Fungal Diversity 51: 279–296. https://doi.org/10.1007/s13225-011-0136-7