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Issue: Vol. 653 No. 1: 13 Jun. 2024
Type: Article
Published: 2024-06-13
DOI: 10.11646/phytotaxa.653.1.3
Page range: 37-53
Abstract views: 35
PDF downloaded: 2

A combined phylogenetic and numerical taxonomic study of Matthiola montana and M. anchoniifolia (Brassicaceae)

Department of Plant and Animal Production, Ahi Evran University, Kırşehir, Türkiye
Department of Biology, Faculty of Science, Hacettepe University, Ankara, Türkiye. Hacettepe University, Biological Diversity Research and Application Center, Ankara, Türkiye.
Brassicaeae Matthiola anchoniifolia M. montana morphology phylogeny Eudicots

Abstract

Matthiola montana and M. anchoniifolia are endemic to Türkiye and can be distinguished from each other by morphological features including siliques, leaves, petals, and hairs. Although these two taxa have traditionally been treated as separate species in all taxeonomic accounts, field and herbarium studies together with findings in the literature suggest that they should be considered at the subspecies level at the very least due to transitions in the aforementioned characters. To test this hypothesis, we evaluated all available herbarium specimens and specimens from field collections, conducting numerical and phylogenetic analyses based on the ITS region of the nuclear DNA. Our analyses, including the unweighted pair group method with arithmetic mean (UPGMA) and principal coordinate analysis (PCoA) as well as phylogenetic methods, consistently indicated that the two species did not form separate clusters. Therefore, our careful examination of the material, along with morphological and phylogenetic analyses, strongly supports the conclusion that M. anchoniifolia is not distinct from M. montana and should be a synonym of the latter.

References

  1. Al-Shehbaz, I.A., Beilstein, M.A. & Kellogg, E.A. (2006) Systematics and phylogeny of the Brassicaceae (Cruciferae): an overview. Plant Systematics and Evolution 259: 89–120. https://doi.org/10.1007/s00606-006-0415-z
  2. Al-Shehbaz, I.A. & Barriera, G. (2019) Typification of Edmond Boissier’s Cruciferae (Brassicaceae) names enumerated in Flora Orientalis. Boissiera 72: 1–192. Available from: https://www.ville-ge.ch/cjb/publications/Boissiera72/pdf/Boissiera72.pdf (accessed: 23 September 2023).
  3. Álvarez, I.J.F.W. & Wendel, J.F. (2003) Ribosomal ITS sequences and plant phylogenetic inference. Molecular Phylogenetics and Evolution 29 (3): 417–434. https://doi.org/10.1016/S1055-7903(03)00208-2
  4. Appel, O. & Al-Shehbaz, I.A. (2003) Matthiola R.Br. In: Kubitzki, K. & Bayer, C. (Eds.) The families and genera of vascular plants. Flowering plants, dicotyledons: Malvales, Capparales, and non-betalanin Caryophyllales, vol. 5. Springer-Verlag, Berlin, pp. 140.
  5. Boissier, E. (1842) Plantae aucherianae orientales enumeratae, cum novarum specierum descriptione. Annales des sciences naturelles, botanique, Sér. 2 17: 45–90, 150–205, 381–390.
  6. Boissier, E. (1844) Diagnoses plantarum Orientalium novarum. Ser. 2, 5. B. Herrmann, Lipsiae [Leipzig] & Typis Ramboz et Schuchardt, Genève.
  7. Boissier, E. (1856) Diagnoses plantarum Orientalium novarum. Ser. 1, 5. B. Herrmann, Lipsiae [Leipzig] & Typis Ramboz et Schuchardt, Genève, 91 pp.
  8. Candolle, A.P. (1821) Regni vegetabilis systema naturale, Vol. 2. Treuttel & Würtz, Strasbourg, London, 576 pp.
  9. Collins, T.J. (2007) ImageJ for microscopy. Biotechniques 43 (S1): S25–S30. https://doi.org/10.2144/000112517
  10. Cullen, J. (1965) Matthiola R. Br. In: Davis, P.H. (Ed.) Flora of Turkey and the East Aegean Islands, vol. 1. Edinburgh University Press, Edinburgh, pp. 447–450.
  11. Davis, P.H. (1971) Distribution patterns in Anatolia with particular reference to endemism. In: Davis, P.H., Harper, P.C. & Hedge, I.C. (Eds.) Plant life of South-West Asia. Botanical Society of Edinburgh, Edinburgh, pp. 15–27.
  12. Depypere, L., Chaerle, P., Breyne, P., Vander Mijnsbrugge, K. & Goetghebeur, P. (2009) A combined morphometric and AFLP based diversity study challenges the taxonomy of the European members of the complex Prunus L. section Prunus. Plant systematics and evolution 279: 219–231. https://doi.org/10.1007/s00606-009-0158-8
  13. Doğan, B., Çelik, M., Ünal, M., Sefali, A., Martin, E. & Kaya, A. (2016) Study of phylogenetic relationship of Turkish species of Matthiola (Brassicaceae) based on ISSR amplification. Turkish journal of botany 40 (2): 130–136. https://doi.org/10.3906/bot-1412-34
  14. Dumlupınar, İ., Gür, H. & Özüdoğru, B. (2023) Distributional ecology of Noccaea vesicaria in relation to the Anatolian Diagonal. Turkish journal of botany 47 (4): 267–277. https://doi.org/10.55730/1300-008X.2765
  15. Erden, A. & Menemen, Y. (2019) Geçmişten günümüze Cruciferae (Lahanagiller) familyası: sistematiği ve filogenisi. Bağbahçe bilim dergisi 6 (2): 10–24. https://doi.org/10.35163/bagbahce.613718
  16. German, D.A. (2019) Nomenclatural notes on Cruciferae. Turczaninowia 22 (4): 210–219. https://doi.org/10.14258/turczaninowia.22.4.18
  17. German, D.A., Friesen, N., Neuffer, B., Al-Shehbaz, I.A. & Hurka, H. (2009) Contribution to ITS phylogeny of the Brassicaceae, with special reference to some Asian taxa. Plant systematics and evolution 283 (1): 33–56. https://doi.org/10.1007/s00606-009-0213-5
  18. German, D.A., Hendriks, K.P., Koch, M.A., Lens, F., Lysak, M.A., Bailey, C.D., Mummenhoff, K. & Al-Shehbaz, I.A. (2023) An updated classification of the Brassicaceae (Cruciferae). PhytoKeys 220: 127–144. https://doi.org/10.3897/phytokeys.220.97724
  19. Govaerts, R., Nic Lughadha, E., Black, N., Turner, R. & Paton, A. (2021) The World Checklist of Vascular Plants, a continuously updated resource for exploring global plant diversity. Scientific data 8 (1): 215. https://doi.org/10.6084/m9.figshare.15035046
  20. Gowler, Z.R. (1998) A taxonomic revision of the genus Matthiola R. Br. (Cruciferae) and related genera. PhD thesis, University of Edinburgh, Edinburgh.
  21. Guindon, S. & Gascuel, O. (2003) A simple, fast and accurate method to estimate large phylogenies by maximum-likelihood. Systematic biology 52: 696–704. https://doi.org/10.1080/10635150390235520
  22. Güner, A., Aslan, S., Ekim, T., Vural, M. & Babaç, M.T. (2012) Türkiye Bitkileri Listesi (damarlı bitkiler). Nezahat Gökyiğit Botanik Bahçesi ve Flora Araştırmaları Derneği Yayını, Istanbul, 658 pp.
  23. Gür, H. (2016) The Anatolian diagonal revisited: testing the ecological basis of a biogeographic boundary. Zoology in the Middle East 62: 189–199. https://doi.org/10.1080/09397140.2016.1226544
  24. Hammer, Ø., Harper, D.A. & Ryan, P.D. (2001) PAST: Paleontological statistics software package for education and data analysis. Palaeontologia electronica 4 (1): 9.
  25. Hendriks, K.P., Kiefer, C., Al-Shehbaz, I.A., Bailey, C.D., van Huysduynen, A.H., Nikolov, L.A., Nauheimer, L., Zuntini, A.R., German, D.A., Franzke, A. & Koch, M.A. (2023) Global Brassicaceae phylogeny based on filtering of 1,000-gene dataset. Current biology 33: 4052–4068. https://doi.org/10.1016/j.cub.2023.08.026
  26. Huber-Morath, A. (1963) Novitiae florae Anatolica VI. Bauhinia 2 (2): 192–213.
  27. Jaén-Molina, R., Caujapé-Castells, J., Reyes-Betancort, J.A., Akhani, H., Fernández-Palacios, O., de Paz, J.P., Febles-Hernández, R. & Marrero-Rodríguez, Á. (2009) The molecular phylogeny of Matthiola R. Br. (Brassicaceae) inferred from ITS sequences, with special emphasis on the Macaronesian endemics. Molecular phylogenetics and evolution 53 (3): 972–981. https://doi.org/10.1016/j.ympev.2009.08.031
  28. Kearse, M., Moir, R., Wilson, A., Stones-Havas, S., Cheung, M., Sturrock, S., Buxton, S., Cooper, A., Markowitz, S., Duran, C. & Thierer, T. (2012) Geneious Basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics 28 (12): 1647–1649. https://doi.org/10.1093/bioinformatics/bts199
  29. Koenen, E.J., Ojeda, D.I., Steeves, R., Migliore, J., Bakker, F.T., Wieringa, J.J., Kidner, C., Hardy, O.J., Pennington, R.T., Bruneau, A. & Hughes, C.E. (2019) Large-scale genomic sequence data resolve the deepest divergences in the legume phylogeny and support a near-simultaneous evolutionary origin of all six subfamilies. New phytologist 225: 1355–1369. https://doi.org/10.1111/nph.16290
  30. Koopman, W.J.M., Wissemann, V., De Cock, K., Van Huylenbroeck, J., De Riek, J., Sabatino, G.J.H., Visser, D., Vosman, B., Ritz, C.M. & Maes, B. & Werlemark, G. (2008) AFLP markers as a tool to reconstruct complex relationships: a case study in Rosa (Rosaceae). American journal of botany 95 (3): 353–366. https://doi.org/10.3732/ajb.95.3.353
  31. Letunic, I. & Bork, P. (2016) Interactive tree of life (iTOL) v3: an online tool for the display and annotation of phylogenetic and other trees. Nucleic acids research 44 (W1): W242–W245. https://doi.org/10.1093/nar/gkw290
  32. Martin, E., Ünal, M., Doğan, B., Altınordu, F., Sefali, A. & Kaya, A. (2016) Karyotype analyses of the genus Matthiola (Brassicaceae) in Turkey. Cytologia 81 (1): 53–60. https://doi.org/10.1508/cytologia.81.53
  33. Martin, E., Yılmaz, G. & Tekin, M. (2013) Karyology of endemic Matthiola anchoniifolia Hub.-Mor.(Brassicaceae) in Turkey. Caryologia 66 (4): 293–295. https://doi.org/10.1080/00087114.2013.852343
  34. Miller, M.A., Pfeiffer, W. & Schwartz, T. (2011) The CIPRES science gateway: a community resource for phylogenetic analyses. In: Proceedings of the 2011 TeraGrid Conference: Extreme digital discovery. Salt Lake City, pp. 1–8. https://doi.org/10.1145/2016741.2016785
  35. Mummenhoff, K., Franzke, A. & Koch, M. (1997) Molecular phylogenetics of Thlaspi s.l. (Brassicaceae) based on chloroplast DNA restriction site variation and sequences of the internal transcribed spacers of nuclear ribosomal DNA. Canadian journal of botany 75: 469–482. https://doi.org/10.1139/b97-051
  36. Mutlu, B. (2012) Matthiola Aiton. In: Güner, A., Aslan, S., Ekim, T., Vural, M. & Babaç, M.T. (Eds.) Türkiye Bitkileri Listesi (damarlı bitkiler). Nezahat Gökyiğit Botanik Bahçesi ve Flora Araştırmaları Derneği Yayını, Istanbul, pp. 287–288.
  37. Nilsson, R.H., Ryberg, M., Kristiansson, E., Abarenkov, K., Larsson, K.H. & Kõljalg, U. (2006) Taxonomic reliability of DNA sequences in public sequence databases: a fungal perspective. PLoS One 1 (1): e59. https://doi.org/10.1371/journal.pone.0000059
  38. Radford, A.E. (1986) Fundamentals of plant systematics. Harper and Row, New York. 498 pp.
  39. Rambaut, A. & Drummond, A.J. (2013) Tracer v1.5. University of Edinburgh, Edinburgh. Available from: http://beast.bio.ed.ac.uk (accessed 23 September 2023)
  40. Reddy, S., Kimball, R.T., Pandey, A., Hosner, P.A., Braun, M.J., Hackett, S.J., Han, K.L., Harshman, J., Huddleston, C.J., Kingston, S. & Marks, B.D. (2017) Why do phylogenomic data sets yield conflicting trees? Data type influences the avian tree of life more than taxon sampling. Systematic biology 66: 857–879. https://doi.org/10.1093/sysbio/syx041
  41. Rešetnik, I., Satovic, Z., Schneeweiss, G.M. & Liber, Z. (2013) Phylogenetic relationships in Brassicaceae tribe Alysseae inferred from nuclear ribosomal and chloroplast DNA sequence data. Molecular phylogenetics and evolution 69 (3): 772–786. https://doi.org/10.1016/j.ympev.2013.06.026
  42. Ronquist, F. & Huelsenbeck, J.P. (2003) MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19 (12): 1572–1574. https://doi.org/10.1093/bioinformatics/btg180
  43. Saitou, N. & Nei, M. (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular biology and evolution 4: 406–425. https://doi.org/10.1093/oxfordjournals.molbev.a040454
  44. Sánchez, J.L., Domina, G. & Caujapé-Castells, J. (2005) Genetic differentiation of three species of Matthiola (Brassicaceae) in the Sicilian insular system. Plant systematics and evolution 253: 81–93. https://doi.org/10.1007/s00606-005-0307-7
  45. Smith, A.B. (1994) Rooting molecular trees: problems and strategies. Biological journal of the Linnean Society 51 (3): 279–292. https://doi.org/10.1111/j.1095-8312.1994.tb00962.x
  46. Sun, M., Folk, R.A., Gitzendanner, M.A., Soltis, P.S., Chen, Z., Soltis, D.E. & Guralnick, R.P. (2020) Estimating rates and patterns of diversification with incomplete sampling: a case study in the rosids. American journal of botany 107 (6): 895–909. https://doi.org/10.1002/ajb2.1479
  47. Stamatakis, A. (2014) RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 30: 1312–1313. https://doi.org/10.1093/bioinformatics/btu033
  48. Taberlet, P., Gielly, L., Pautou, G. & Bouvet, J. (1991) Universal primers for amplification of three non-coding regions of chloroplast DNA. Plant molecular biology 17 (5): 1105–1109. https://doi.org/10.1007/BF00037152
  49. Tekin, M., Yilmaz, G. & Martin, E. (2013) Morphological, anatomical and palynological studies on endemic Matthiola anchoniifolia Hub.-Mor.(Brassicaceae). Notulae scientia biologicae 5 (2): 163–168. https://doi.org/10.15835/nsb529075
  50. Thiers, B. (2023 [continuously updated]) Index Herbariorum: A global directory of public herbaria and associated staff. New York Botanical Garden, New York. Available from: https://sweetgum.nybg.org/ih/ (accessed: 23 September 2023).
  51. Thomas, J.A., Trueman, J.W., Rambaut, A. & Welch, J.J. (2013) Relaxed phylogenetics and the Palaeoptera problem: resolving deep ancestral splits in the insect phylogeny. Systematic biology 62: 285–297. https://doi.org/10.1093/sysbio/sys093
  52. Turland, N.J., Wiersema, J.H., Barrie, F.R., Greuter, W., Hawksworth, D.L., Herendeen, P.S., Knapp, S., Kusber, W.-H., Li, D.-Z., Marhold, K., May, T.W., McNeill, J., Monro, A.M., Prado, J., Price, M.J. & Smith, G.F. (Eds.) (2018) International Code of Nomenclature for algae, fungi, and plants (Shenzhen Code) adopted by the Nineteenth International Botanical Congress Shenzhen, China, July 2017. Regnum Vegetabile 159. Koeltz Botanical Books, Glashütten. https://doi.org/10.12705/Code.2018
  53. Ward, J.M. (1993) Systematics of New Zealand Inuleae (Compositae-Asteraceae)—A numerical phenetic study of Raoulia in relation to allied genera. New Zealand journal of botany 31: 29–42. https://doi.org/10.1080/0028825X.1993.10419532
  54. Warwick, S.I., Sauder, C.A., Al-Shehbaz, I.A. & Jacquemoud, F. (2007) Phylogenetic relationships in the tribes Anchonieae, Chorisporeae, Euclidieae, and Hesperideae (Brassicaceae) based on nuclear ribosomal ITS DNA sequences. Annals of the Missouri Botanical Garden 94 (1): 56–78. https://doi.org/10.3417/0026-6493(2007)94[56:PRITTA]2.0.CO;2
  55. White, T.J., Bruns, T., Lee, S. & Taylor, J. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis, M.A., Gelfand, D.H., Sninsky, J.J. & White, T.J. (Eds.) PCR protocols: a guide to methods and applications. Academic Press, New York, pp. 315–322.
  56. Wu, H.Y., Chan, K.T., But, G.W.C. & Shaw, P.C. (2021) Assessing the reliability of medicinal Dendrobium sequences in GenBank for botanical species identification. Scientific Reports 11 (1): 3439. https://doi.org/10.1038/s41598-021-82385-z
  57. Yıldırım, H., Özdöl, T., Can, I.D., Erdem, S., Oğur, E. & Özüdoğru, B. (2022) Knautia goecmenii (Caprifoliaceae): an unexpected caespitose suffrutescent new species from the Western Anatolia, Turkey. Phytotaxa 531 (2): 97–110. https://doi.org/10.11646/phytotaxa.531.2.2