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Issue: Vol. 646 No. 2: 2 May 2024
Type: Article
Published: 2024-05-02
DOI: 10.11646/phytotaxa.646.2.3
Page range: 125-142
Abstract views: 4
PDF downloaded: 1

Molecular phylogenetic studies on Asparagus (Asparagaceae) revealed the position of the highly endangered Irano-Turkish halophytic species A. lycaonicus P.H.Davis related to A. officinalis L.

Halophytes and C4 Plants Research Laboratory, Department of Plant Sciences, School of Biology, College of Sciences, University of Tehran, P.O. Box 14155-6455, Tehran, Iran
Halophytes and C4 Plants Research Laboratory, Department of Plant Sciences, School of Biology, College of Sciences, University of Tehran, P.O. Box 14155-6455, Tehran, Iran
Environmental Health Program, Şabanözü Vocational School, Çankırı Karatekin University, P.O. Box 18650, Çankırı, Türkiye
Halophytes and C4 Plants Research Laboratory, Department of Plant Sciences, School of Biology, College of Sciences, University of Tehran, P.O. Box 14155-6455, Tehran, Iran, Botanischer Garten und Botanisches Museum Berlin, Freie Universität Berlin, Königin-Luise-Straße 6-8, 14195 Berlin, Germany
Monocots Bolluk Salt Lake cultivated Asparagus Flora of Iran Flora of Turkey Meyghan Salt Lake

Abstract

Asparagus lycaonicus P.H.Davis (Asparagaceae), a highly interesting halophytic species was found in two disjunct populations of ca. 1550 km aerial distance, one in Central Anatolia (Bolluk Salt Lake) and the other in Central Iran (Meyghan Salt Lake). Using one nuclear phytochrome C (PHYC) and two plastid regions (trnH-psbA and trnD-T) the phylogenetic position of this Irano-Turkish endemic species was studied. In contrast to low resolution of plastid sequences, the PHYC provides good resolution for understanding species level phylogeny in SW Asian Asparagus. Molecular data confirms a conspecific position of the two disjunct localities in Turkey and Iran and their position in a clade containing cultivated Asparagus and many other Irano-Turkish species. Asparagus lycaonicus is extremely rare in Iran and since its discovery in 1986, it has never been rediscovered in Iran, even in its initial collection site. Intensive land use activities, water level changes and the passage of Arak wastewater into the Meyghan Salt Lake area has drastically changed the habitat of A. lycaonicus and might have caused its extinction. Since its discovery in 1960 in Turkey, the species is only found in a small area around and inside a sodic extraction factory around Bolluk Lake. Considering the critical condition of its populations in Iran and Turkey, we evaluate the conservation status of Asparagus lycaonicus as Critically Endangered (CR). In conclusion we urge the authorities to implement in situ measures for conservation and restoration of the habitats of A. lycaonicus, a halophytic species related to cultivated Asparagus. The taxonomic status of some newly described Asparagus from Iran is discussed with reducing A. touranensis Hamdi & Assadi to synonym of A. griffithii Baker., A. khorasanensis Hamdi & Assadi to synonym of A. breslerianus Schult. & Schult.f, and Asparagus bojnurdensis Hamdi & Assadi to synonym of A. verticillatus L.

References

  1. Akhani, H. (1988) Plant records from Kavire-Meyghan (Arak), new to Iran. Iranian Journal of Botany 4 (1): 105–107.
  2. Akhani, H. (1998) Plant biodiversity of Golestan National Park, Iran. Stapfia 53: 1–411
  3. Akhani, H. (2002) Notes on the flora of Iran 1: Asparagus (Asparagaceae) and Nitraria (Zygophyllaceae). Edinburgh Journal of Botany 59 (2): 295–302. https://doi.org/10.1017/S0960428602000112
  4. Akhani, H. (2005) The Illustrated Flora of Golestan National Park, Iran. Vol. 1. Tehran University Press, Tehran, 481 pp.
  5. Akhani, H., Edwards, G. & Roalson, E.H. (2007) Diversification of the Old World Salsoleae s.l. (Chenopodiaceae): Molecular phylogenetic analysis of nuclear and chloroplast data sets and a revised classification. International Journal of Plant Sciences 168 (6): 931–956. https://doi.org/10.1086/518263
  6. Assadi, M. (2023) Asparagaceae. In: Assadi & Maassoumi, A.A. (Eds.) Flora of Iran, 174. Research Institute of Forests and Rangelands, Iran, 22 pp.
  7. Baker, J.G. (1875) Revision of the genera and species of the Asparagaceae. Journal of the Linnean Society of London 14: 594–629. https://doi.org/10.1111/j.1095-8339.1875.tb00349.x
  8. Browicz, K. (1990) Asparagus [Liliaceae II]. In: Rechinger, K.H. (Ed.) Flora Iranica, vol 165. Graz, Austria, pp. 167–176.
  9. CITES (1983) Convention on International Trade in Endangered Species of Wild Fauna and Flora. Annex-II, Annexes, pp. 135–146.
  10. Council of Europe (1999) Appendices to the Convention on the Conservation of European Wildlife and Natural Habitats. Secretariat Memorandum prepared by the Directorate of Environment and Local Authorities, Strasbourg, 26 pp.
  11. Darriba, D., Taboada, G.L., Doallo, R. & Posada, D. (2012) jModelTest 2: more models, new heuristics and parallel computing. Nature Methods 9 (8): 772. https://doi.org/10.1038/nmeth.2109
  12. Davis, P.H. (1983) Materials for a Flora of Turkey, XXXVIII. Araceae, Dioscoreaceae, Liliaceae. Notes from the Royal Botanic Garden, Edinburgh 41 (1): 45–54.
  13. Davis, P.H. (1984) Asparagus. In: Davis, P.H. (Ed.) Flora of Turkey and the East Aegean Islands, vol 8. UK: Edinburgh University Press, Edinburgh, pp. 75–81.
  14. Doyle, J. & Doyle, J. (1987) A rapid procedure for DNA purification from small quantities of fresh leaf tissue. Phytochemical Bulletin 19: 11–15.
  15. Druce, G.C. (1913) Supplement to botanical exchange club report 1913. Report/Botanical Society and Exchange Club of the British Isles 3: 405–426.
  16. Ekim, T., Koyuncu, M., Vural, M., Duman, H., Aytaç, Z. & Adigüzel, N. (2000) Türkiye Bitkileri Kırmızı Listesi, (Red Data Book of Turkish Plants (Pteridophyta and Angiospermae). Türkiye Tabiatını Koruma Derneği ve Van 100. Yıl Üniversitesi Yayını, 246 Syf. Ankara. [In Turkish]
  17. Güner, A., Aslan, S., Ekim, T., Vural, M. & Babaç, M.T. (Eds.) (2012) Türkiye Bitkileri Listesi (Damarlı Bitkiler). Nezahat Gökyiğit Botanik Bahçesi ve Flora Araştırmaları Derneği Yayını İstanbul, 761 pp. [In Turkish]
  18. Güvenç, A. (1996) “Türkiye’de yetişen Asparagus (Kuşkonmaz) türleri üzerinde farmasötik botanik yönünden araştırmalar. Ankara. [In Turkish]
  19. Hajiboland, R., Bahrami-Rad, S., Akhani, H. & Poschenrieder, C. (2018) Salt tolerance mechanisms in three Irano-Turanian Brassicaceae halophytes relatives of Arabidopsis thaliana. Journal of Plant Research 131 (6): 1029–1046. https://doi:10.1007/s10265-018-1053-6
  20. Hamdi, S. & Assadi, M. (2009) Asparagus khorasanensis (Asparagaceae), a new species from Iran. Feddes Repertorium 120 (7–8): 419–425. https://doi.org/10.1002/fedr.200911120
  21. Hamdi, S., Assadi, M. & Jouharchi, M. (2017) Asparagus bojnurdensis (Asparagaceae), a new species from Iran. Kew Bulletin 72: 1–6. https://doi.org/10.1007/s12225-017-9691-8
  22. Hamdi, S.M.M. (2016) A new record of Asparagus (Asparagaceae) from Iran. Iranian Journal of Botany 22 (1): 30–32.
  23. Hamdi, S.M.M. & Assadi, M. (2013) A new species of Asparagus L. (Asparagaceae) from Iran. Iranian Journal of Botany 19 (1): 44–46.
  24. Hamdi, S.M.M. & Assadi, M. (2017) Asparagus azerbaijanensis, a new species of Asparagus subgen. Asparagopsis (Asparagaceae) from Iran. Phytotaxa 297 (1): 93–96. https://doi.org/10.11646/phytotaxa.297.1.12
  25. Hernandez, L. (1995) Taxonomic study of the Mexican genus Hemiphylacus (Hyacinthaceae). Systematic Botany 20 (4): 546–554. https://doi.org/10.2307/2419808
  26. IUCN (2012) IUCN Red List Categories and Criteria: Version 3.1. Second edition. IUCN Species Survival Commission, Gland, Switzerland and Cambridge, 30 pp.
  27. Jessop, J.P. (1966) The genus Asparagus in South Africa. Bothalia 9 (1): 31–96. https://doi.org/10.4102/abc.v9i1.1576
  28. Kapoor, M., Mawal, P., Sharma, V. & Gupta, R.C. (2020) Analysis of genetic diversity and population structure in Asparagus species using SSR markers. Journal of Genetic Engineering and Biotechnology 18 (50): 1–10. https://doi.org/10.1186/s43141-020-00065-3
  29. Kelchner, S.A. (2000) The evolution of noncoding chloroplast DNA and its application in plant systematics. Annals of the Missouri Botanical Gardens 87: 482–498. https://doi.org/10.2307/2666142
  30. Kubitzki, K. & Rudall, P. (1998) Asparagaceae. In: Kubitzki, K. (Ed.) The Families and Genera of Vascular Plants, vol 3. Springer, Berlin Heidelberg, pp. 125–129. https://doi.org/10.1007/978-3-662-03533-7_15
  31. Kunth, K.S. (1850) Enumeratio plantarum omnium hucusque cognitarum: secundum familias naturales disposita, adjectis characteribus, differentiis et synonymis, vol 5. Sumtibus J.G. Cottae, Stutgardiae, 908 pp.
  32. Linnaeus, C. (1753) Species Plantarum, 1, 2. Impensis Laurentii Salvii, Holmiæ, Stockholm, 1200 pp.
  33. Linnaeus, C. (1762) Species Plantarum, vol. 1. Impensis Laurentii Salvii, Holmiæ, Stockholm, 784 pp.
  34. Löhne, C. & Borsch, T. (2005) Molecular evolution and phylogenetic utility of the petD group II intron: a case study in basal angiosperms. Molecular Biology and Evolution 22: 317–332. https://doi.org/10.1093/molbev/msi019
  35. Makino, T. (1907) Observations on the flora of Japan. Botanical Magazine (Tokyo) 21: 161–162. https://doi.org/10.15281/jplantres1887.21.251_161
  36. Merrill, E.D. (1919) Additional notes on the Kwangtung flora. Philippine Journal of Science 15: 225–261.
  37. Mousavizadeh, S.J., Gil, J., Castro, P., Hassandokht, M.R. & Moreno, R. (2021) Genetic diversity and phylogenetic analysis in Asian and European Asparagus subgenus species. Genetic Resources and Crop Evolution 68 (8): 3115–3124. https://doi.org/10.1007/s10722-021-01262-w
  38. Müller, J., Müller, K.F., Neinhuis, C. & Quandt, D. (2010) PhyDE: Phylogenetic data editor.
  39. Murray, J.A. (1774) Systema Vegetabilium, ed. 13 Gottingae [Göttingen].
  40. Norup, M.F., Petersen, G., Burrows, S., Bouchenak-Khelladi, Y., Leebens-Mack, J., Pires, J.C., Linder, H.P. & Seberg, O. (2015) Evolution of Asparagus L. (Asparagaceae): Out-of-South-Africa and multiple origins of sexual dimorphism. Molecular Phylogenetics and Evolution 92: 25–44. https://doi.org/10.1016/j.ympev.2015.06.002
  41. Ronquist, F., Teslenko, M., Van Der Mark, P., Ayres, D.L., Darling, A., Höhna, S., Larget, B., Liu, L., Suchard, M.A. & Huelsenbeck, J.P. (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology 61 (3): 539–542. https://doi.org/10.1093/sysbio/sys029
  42. Schultes, A. & Schultes, H. (1829) Asparagus. Systema vegetabilium ed. 15 7: 317–337.
  43. Shaw, J., Lickey, E.B., Beck, J.T., Farmer, S.B., Liu, W.S., Miller, J., Siripun, K.C., Winder, C.T., Schilling, E.E. & Small, R.L. (2005) The tortoise and the hare II: Relative utility of 21 noncoding chloroplast DNA sequences for phylogenetic analysis. American Journal of Botany 92 (1): 142–166. https://doi.org/10.3732/ajb.92.1.142
  44. Stöver, B.C. & Müller, K.F. (2010) TreeGraph 2: combining and visualizing evidence from different phylogenetic analyses. B M C Bioinformatics 11 (1): 7. https://doi.org/10.1186/1471-2105-11-7
  45. Ürker, O. & Keçeli, T. (2022) A current evaluation on conservation biology properties of Tuzkırgını (Asparagus lycaonicus P.H. Davis) that is an endangered plant species. BEU Journal of Science 9 (4): 1496–1513. [In Turkish] https://doi.org/10.17798/bitlisfen.726779
  46. Watson, S. (1882–1883) Contributions to American Botany. Proceedings of the American Academy of Arts and Sciences 18: 96–196. https://doi.org/10.2307/25138689
  47. Xinqi, C. & Tamanian, K.G. (2000) Asparagus. In: Wu, Z.Y. & Raven, P.H. (Eds.) Flora of China, vol 24. Science Press, Beijing, and Missouri Botanical Garden Press, St. Louis, pp. 208–215.