Skip to main content Skip to main navigation menu Skip to site footer
Responsive image
Issue: Vol. 636 No. 1: 1 Feb. 2024
Type: Article
Published: 2024-02-01
DOI: 10.11646/phytotaxa.636.1.5
Page range: 61-73
Abstract views: 22
PDF downloaded: 1

Molecular phylogeny and morphological characterization revealed Lepiota brunneoaurantia, a new species in L. sect. Stenosporae from Margalla Hills, Pakistan

Department of Botany, Division of Science and Technology, University of Education, Lahore, 54000, Pakistan
Department of Botany, Division of Science and Technology, University of Education, Lahore, 54000, Pakistan
Department of Botany, Division of Science and Technology, University of Education, Lahore, 54000, Pakistan
ITS lepiotaceous fungi nrDNA saprobic taxonomy Fungi

Abstract

Lepiota brunneoaurantia sp. nov. was collected from the Margalla Hills, Pakistan. This species was characterized by an orangish brown pileus with a dark brown umbo, presence of orangish upturned, crowded squamules, close off-white lamellae having lameullae of variable lengths, small (5.6 × 2.5 µm), oblong to ellipsoid basidiospores and clavate cheilocystidia. Comparative study based on morphology and molecular phylogeny revealed that it is distinct from already known taxa in the genus and represent a new species.

References

  1. Ahmad, S., Iqbal, S.H. & Khalid, A.N. (1997) Fungi of Pakistan. Sultan Ahmad Mycological Society of Pakistan, Department of Botany, University of the Punjab, Quaid-e-Azam Campus, Lahore, pp. 1–248.
  2. Ali, S., Khan, S.M., Siddiq, Z., Ahmad, Z., Ahmad, K.S., Abdullah, A., Hashem, A., Al-Arjani, A.F. & Abd_Allah, E.F. (2022) Carbon sequestration potential of reserve forests present in the protected Margalla Hills National Park. Journal of King Saud University-Science 34 (4): 101978. https://doi.org/10.1016/j.jksus.2022.101978
  3. Asif, M., Izhar, A., Haqnawaz, M., Niazi, A.R. & Khalid, A.N. (2022) Lepiota brunneogranulosa (Agaricaceae): a new species from Punjab, Pakistan, based on integrated taxonomy. Acta Botanica Brasilica: 36. https://doi.org/10.1590/0102-33062021abb0382
  4. Bas, C. (1969) Morphology and subdivision of Amanita and a monograph of its section Lepidella. Persoonia-Molecular Phylogeny and Evolution of Fungi 5 (4): 285–573.
  5. Bashir, H., Usman, M. & Khalid, A.N. (2020) Lepiota cholistanensis a new species of Lepiota (Agaricaceae: Basidiomycota) from Cholistan desert, Pakistan. Phytotaxa 455 (4): 267–276. https://doi.org/10.11646/phytotaxa.455.4.4
  6. Bon, M. (1996) Die Groβpilzflora von Europa 3 Lepiotaceae. IHW-Verlag, Eching, Germany. [in German]
  7. Bruns, T.D. (1995) Thoughts on the processes that maintain local species diversity of ectomycorrhizal fungi. Plant and Soil 170: 63–73. https://doi.org/10.1007/BF02183055
  8. Candusso, M. & Lanzoni G. (1990) Lepiota s.l. Fungi Europaei 4. Candusso Edizioni, Saronno, Italy, 743 pp. [in Italian]
  9. Ge, Z.W., Yang, Z.L. & Vellinga, E.C. (2010) The genus Macrolepiota (Agaricaceae, Basidiomycota) in China. Fungal Diversity 45: 81–98. https://doi.org/10.1007/s13225-010-0062-0
  10. Hall, T.J. (1999) BIOEDIT: a user-friendly biological sequence alignment editor and analysis program for windows 95/98/ NT/7. Nucleic Acids Symposium Series 41: 95–98.
  11. He, M.-Q., Zhao, R.-L., Hyde, K.D., Begerow, D., Kemler, M., Yurkov, A., McKenzie, E.H.C., Raspé, O., Kakishima, M., Sánchez-Ramírez, S., Vellinga, E.C., Halling, R., Papp, V., Zmitrovich, I.V., Buyck, B., Ertz, D., Wijayawardene, N.N., Cui, B.-K., Schoutteten, N., Liu, X.-Z., Li, T.-H., Yao, Y.-J., Zhu, X.-Y., Liu, A.-Q., Li, G.-J., Zhang, M.-Z., Ling, Z.-L., Cao, B., Antonín, V., Boekhout, T., da Silva, B.D.B., De Crop, E., Decock, C., Dima, B., Dutta, A.K., Fell, J.W., Geml, J., Ghobad-Nejhad, M., Giachini, A.J., Gibertoni, T.B., Gorjón, S.P., Haelewaters, D., He, S.-H., Hodkinson, B.P., Horak, E., Hoshino, T., Justo, A., Lim, Y.W., Menolli Jr., N., Mešić, A., Moncalvo, J.-M., Mueller, G.M., Nagy, L.G., Nilsson, R.H., Noordeloos, M., Nuytinck, J., Orihara, T., Ratchadawan, C., Rajchenberg, M., Silva-Filho, A.G.S., Sulzbacher, M.A., Tkalčec, Z., Valenzuela, R., Verbeken, A., Vizzini, A., Wartchow, F., Wei, T.-Z., Weiß, M., Zhao, C.-L. & Kirk, P.M.(2019) Notes, outline and divergence times of Basidiomycota.Fungal Diversity 99: 105–367. https://doi.org/10.1007/s13225-019-00435-4
  12. Hou, Y.J. & Ge, Z.W. (2020) New species of Echinoderma and Lepiota (Agaricaceae) from China. Phytotaxa 44 (4): 221–236. https://doi.org/10.11646/phytotaxa.447.4.1
  13. Iqbal, M., Khan, S.M., Ahmad, Z., Hussain, M., Shah, S.N., Kamran, S., Manan, F., Haq, Z.U. & Ullah, S. (2021) Vegetation classification of the Margalla foothills, Islamabad under the influence of edaphic factors and anthropogenic activities using modern ecological tools. Pakistan Jounral of Botany 53 (5): 1831–1843. https://doi.org/10.30848/PJB2021-5(22)
  14. Kirk, P.M., Cannon, P.F., Minter, D.W. & Stalpers, J.A. (2008) Ainsworth and Bisby’s Dictionary of the fungi. Wallingford, International Mycological Institute, 771 pp. https://doi.org/10.1079/9780851998268.0000
  15. Kumar, T.K.A. & Manimohan, P. (2009) The genus Lepiota (Agaricales, Basidiomycota) in Kerala State, India. Mycotaxon 107 (1): 105–138. https://doi.org/10.5248/107.105
  16. Liang, J.F. & Yang, Z.L. (2016) Taxonomy and phylogeny in Lepiota sect. Stenosporae from China. Mycologia 108 (1): 56–69. https://doi.org/10.3852/14-192
  17. Liang, J.F., Xu, J. & Yang, Z.L. (2009) Divergence, dispersal and recombination in Lepiota cristata from China. Fungal Diversity 38: 105–124.
  18. Liang, J.F., Yang, Z.L., Xu, J. & Ge, Z.W. (2010) Two new unusual Leucoagaricus species (Agaricaceae) from tropical China with blue-green staining reactions. Mycologia 102 (5): 1141–1152. https://doi.org/10.3852/09-021
  19. Naseer, A., Jabeen, S., Khanum, S., Waseem, B. & Khalid, A.N. (2022) Lepiota lilacea (Agaricales, Basidiomycota), a New Record from Pakistan. Chiang Mai Journal of Science 49 (3): 693–700. https://doi.org/10.12982/CMJS.2022.054
  20. Nawaz, R., Khalid, A.N., Hanif, M. & Razaq, A. (2013) Lepiota vellingana sp. nov. (Basidiomycota, Agaricales) a new species from Lahore, Pakistan. Mycological Progress 12: 727–732. https://doi.org/10.1007/s11557-012-0884-0
  21. Niazi, A.R., Asif, M., Izhar, A. & Khalid, A.N. (2021) A new species of Lepiota (Agaricaceae) from Punjab, Pakistan. Phytotaxa 511 (2): 163–174. https://doi.org/10.11646/phytotaxa.511.2.4
  22. Orton, P.D. (1960) New check list of British Agarics and Boleti. Part III. Notes on genera and species in the list. Transactions of the British Mycological Society 43 (2): 159–439. https://doi.org/10.1016/S0007-1536(60)80065-4
  23. Prylutskyi, O., Zinenko, O. & Havrysh, P. (2021) First records of three Lepiota species (Agaricales, Basidiomycota) from Ukraine, with notes on a poorly known species, Lepiota subalba. Ukrainian Botanical Journal 78 (6): 373–380. https://doi.org/10.15407/ukrbotj78.06.373
  24. Qasim, T., Khalid, A.N., Vellinga, E.C. & Razaq, A. (2015) Lepiota albogranulosa sp. nov. (Agaricales, Agaricaceae) from Lahore, Pakistan. Mycological Progress 14: 1–6. https://doi.org/10.1007/s11557-015-1037-z
  25. Qasim, T., Khalid, A. & Vellinga, E. (2016) A new species of Lepiota, Lepiota lahorensis, from Lahore, Pakistan. Turkish Journal of Botany 40 (4): 419–426. https://doi.org/10.3906/bot-1507-31
  26. Quélet, L. (1881) Quelques espèces critiques ou nouvelles de la Flore Mycologique de France (1). Comptes Rendus de l’Association Française pour l’Avancement des Sciences 9: 661–675. [https://gallica.bnf.fr/ark:/12148/bpt6k201156z/f758.item]
  27. Razaq, A., Khalid, A.N. & Ilyas, S. (2013) Molecular identification of Lepiota acutesquamosa and L. cristata (Basidiomycota, Agaricales) based on ITS–rDNA barcoding from Himalayan moist temperate forests of Pakistan. International Journal of Agriculture & Biology 15 (2).
  28. Razaq, A., Khalid, A.N. & Vellinga, E.C. (2012) Lepiota himalayensis (Basidiomycota, Agaricales), a new species from Pakistan. Mycotaxon 121: 319–325. https://doi.org/10.5248/121.319
  29. Razaq, A., Vellinga, E.C., Ilyas, S. & Khalid, A.N. (2014) Lepiota brunneoincarnata and L. subincarnata: distribution and phylogeny. Mycotaxon 126: 133–141. https://doi.org/10.5248/126.133
  30. Razzaq, F., Saba, M., Niazi, A.R. & Khalid, A.N. (2022) Two new records of genus Lepiota (Agaricaceae) from Pakistan. Biology Bulletin 49 (5): 458–465. https://doi.org/10.1134/S1062359022050181
  31. Sarawi, S., Shi, Y.N., Lotz-Winter, H., Reschke, K., Bode, H.B. & Piepenbring, M. (2022) Occurrence and chemotaxonomical analysis of amatoxins in Lepiota spp. (Agaricales). Phytochemistry 195: 113069. https://doi.org/10.1016/j.phytochem.2021.113069
  32. Singer, R. (1986) The Agaricales in Modern Taxon. Koenigstei, Koltez Scientific Books, 4th ed, pp. 1–981.
  33. Sysouphanthong, P., Guo, J.Y., Hyde, K.D., Xu, J.C. & Mortimer, P.E. (2016) Lepiota thailandica Agaricaceae, a new species from Thailand. Phytotaxa 245 (4): 262–270. https://doi.org/10.11646/phytotaxa.245.4.3
  34. Sysouphanthong, P., Hyde, K.D., Chukeatirote, E. & Vellinga, E.C. (2011) A review of genus Lepiota and its distribution in east Asia. Current Research in Environment and Applied Mycology 2: 161–176.
  35. Tamura, K., Stecher, G., Peterson, D., Filipski, A. & Kumar, S. (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution 30 (12): 2725–2729. https://doi.org/10.1093/molbev/mst197
  36. Velenovský, J. (1920) České Houby 2: 201–424. [https://www.biodiversitylibrary.org/item/21925#page/211/mode/1up]
  37. Vellinga, E.C. (2001) Studies in Lepiota III., Some species from California, USA. Mycotaxon 80: 285–295.
  38. Vellinga, E.C. (2003) Phylogeny of Lepiota (Agaricaceae)—evidence from nrITS and nrLSU sequences. Mycological Progress 2: 305–322. https://doi.org/10.1007/s11557-006-0068-x
  39. Vellinga, E.C. (2004) Genera in the family Agaricaceae: evidence from nrITS and nrLSU sequences. Mycological Research 108: 354–377. https://doi.org/10.1017/S0953756204009700
  40. Vellinga, E.C. (2006) Lepiotaceous fungi in California, USA 2-Lepiota rhodophylla sp. nov. Mycotaxon 98: 205–212.
  41. White, T.J., Bruns, T., Lee, S.J.W.T. & Taylor, J. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR protocols: A Guide to Methods and Applications 18 (1): 315–322. https://doi.org/10.1016/B978-0-12-372180-8.50042-1