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Type: Article
Published: 2024-01-26
Page range: 206-216
Abstract views: 931
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Tuber itzcuinzapotl sp. nov. (Tuberaceae, Pezizomycetes), the first edible truffle reported from Mexico with traditional biocultural importance

Colegio de Postgraduados, Campus Montecillo, Edafología, Km 36.5, 56230, Montecillo, Texcoco, Estado de México, Mexico.
Tecnológico Nacional de México. Instituto Tecnológico Superior de Zongolica, Km 4 Carretera a la Compañía S/N, 95000, Tepetitlanapa, Zongolica, Veracruz, Mexico
Tecnológico Nacional de México. Instituto Tecnológico de Ciudad Victoria, Tecnológico Nacional de México, Herbario Micológico José Castillo, Boulevard Emilio Portes Gil No. 1301, 87010 Ciudad Victoria, Tamaulipas, Mexico.
Colegio de Postgraduados, Campus Montecillo, Edafología, Km 36.5, 56230, Montecillo, Texcoco, Estado de México, Mexico
Tecnológico Nacional de México. Instituto Tecnológico Superior de Zongolica, Km 4 Carretera a la Compañía S/N, 95000, Tepetitlanapa, Zongolica, Veracruz, Mexico
Colegio de Postgraduados, Campus Montecillo, Edafología, Km 36.5, 56230, Montecillo, Texcoco, Estado de México, Mexico.
biocultural erosion ethnomycology hypogeous fungi Mexican truffles mycorrhizal fungi traditional knowledge Fungi

Abstract

Tuber itzcuinzapotl is described as a new species to science. This species is characterized by its pale brown ascomata, finely granular peridium, pale brown to gray gleba, and 22–52 × 15–40 µm alveolate ascospores. The new species is putatively associated with Pinus patula in conifer mixed forests in the state of Veracruz, located in eastern Mexico. Phylogenetic analysis based on the nrITS region places the new species in the Maculatum clade, closely related, but morphologically distinctive, to T. miquihuanense and T. mexiusanum. The new species consumed by the Nahua people, traditionally named “Itzcuinzapotl”, constitutes the first edible truffle with biocultural importance in Mexico. Macro- and micromorphological characterization, results of phylogenetic analysis, and photographs are presented. Ethnomycological aspects related to the species are also briefly discussed.

References

  1. Bonito, G.M., Gryganskyi, A.P., Trappe, J.M. & Vilgalys, R. (2010) A global meta‐analysis of Tuber ITS rDNA sequences: species diversity, host associations and long‐distance dispersal. Molecular Ecology 19 (22): 4994–5008. https://doi.org/10.1111/j.1365-294X.2010.04855
  2. Bonito, G., Smith, M.E., Nowak, M., Healy, R.A., Guevara, G., Cázares, E., Kinoshita, A., Nouhra, E.R., Domínguez, L.S., Tedersoo, L., Murat, C., Wang, Y., Arroyo-Moreno, B., Pfister, D.H., Nara, K., Zambonelli, A., Trappe, J.M. & Vilgalys, R. (2013) Historical Biogeography and Diversification of Truffles in the Tuberaceae and Their Newly Identified Southern Hemisphere Sister Lineage. PLoS ONE 8 (1): e52765. https://doi.org/10.1371/journal.pone.0052765
  3. Castellano, M.A., Trappe, J.M., Maser, Z. & Maser, S. (19896) Key to spores of the genera of hypogeous fungi of North America, with reference to animal mycophagy. Mad River Press, Eureka, 185 pp.
  4. Cázares, E., Guevara, G., García, J. & Trappe, J.M. (2008) Melanogaster minysporus sp. nov., a new sequestrate member of the Boletales from Mexico. Revista Mexicana de Micología 28: 67–69.
  5. Cázares, E., García, J., Castillo, J. & Trappe, J.M. (1992) Hypogeous fungi from northern México. Mycologia 84: 341–359.
  6. Contreras, L., Vázquez, A. & Ruan-Soto, F. (2018) Etnomicología y venta de hongos en un mercado del Noroeste del estado de Puebla, México. Scientia Fungorum 47: 47–55.
  7. De Avila, A., Welden, A. & Gúzman, G. (1980) Notes on the ethnomycology of Hueyapan, Morelos Mexico. Journal of Ethnopharmacology 2: 311–321.
  8. García Jiménez, J. (2013) Diversidad de macromicetos en el Estado de Tamaulipas, México Diversity of macrofungi in the Tamaulipas State, México. Doctoral dissertation, Universidad Autónoma de Nuevo León.
  9. García-Jiménez, J., Ayala-Vásquez, O., Guevara-Guerrero, G., Garza-Ocañas, F. & de la Fuente, J.I. (2021) Tuber mixtecorum (Tuberaceae, Pezizales) a new truffle in the Maculatum clade from Mexico. Phytotaxa 509: 113–120. https://doi.org/10.11646/phytotaxa.509.1.6
  10. Guevara, G., Bonito, G. & Cázares, E. (2013a) Revisión del género Tuber (Tuberaceae: Pezizales) de Mexico. Revista Mexicana de Biodiversidad 84: 39–49. https://doi.org/10.7550/rmb.31981
  11. Guevara, G., Bonito, G., Trappe, J.A., Cázares, E., Williams, G., Healy, R., Schadt, C. & Vilgalys, R. (2012) New North American Tuber spp. Mycologia 105: 194–209. https://doi.org/10.3852/12-087
  12. Guevara, G., Bonito, G., Trappe, J.M., Cázares, E., Williams, G., Healy, R.A., Schadt, C. & Vilgalys, R. (2013a) New North American truffles (Tuber spp.) and their ectomycorrhizal associations. Mycologia 105: 194–209. https://doi.org/10.3852/12-087
  13. Guevara, G., Pacioni, G., Leonardi, M., Garza-Ocañas, F. & Gaitán-Hernández, R. (2022) Mycorrhizal synthesis of Périgord black truffle (Tuber melanosporum) with mexican oak species. Microbiology and biotechnology letters 50: 40–50. https://doi.org/10.48022/mbl.2109.09012
  14. Hall, T. (2004) BioEdit v.7.0.1. Available at: Hall, Tom. “BioEdit version 7.0. 0.” Distributed by the author. Available from: https://www.mbio.ncsu.edu/BioEdit/bioedit.html (accessed: 1 August 2023).
  15. Healy, R., Bonito, G.M. & Smith, M.E. (2016) A Brief Overview of the Systematics, Taxonomy, and Ecology of the Tuber rufum Clade. In: Zambonelli, A., Iotti, M., Murat, C. (Eds.) True Truffle (Tuber spp.) in the World. Soil Biology, vol 47. Springer, Cham. pp. 125–136.
  16. https://doi.org/10.1007/978-3-319-31436-5_8
  17. Hu, Y., Karunarathna, S.C., Li, H., Galappaththi, M.C., Zhao, C.L., Kakumyan, P. & Mortimer, P.E. (2022) The Impact of Drying Temperature on Basidiospore Size. Diversity 14 (4): 239.
  18. Instituto Nacional de Estadística y Geografía (2020) Censo de Población y Vivienda 2020. México. Available from: https://www.inegi.org.mx/app/scitel/consultas/index# (Consulted 1 November 2023).
  19. Katoh, K. & Standley, D.M. (2013) MAFFT Multiple Sequence Alignment Software version 7: improvements in performance and usability. Molecular Biology and Evolution 30 (4): 772–780. https://doi.org/10.1093/molbev/mst010
  20. Katoh, K., Misawa, K, Kuma, K. & Miyata, T. (2002) MAFFT: a novel method for rapid multiple sequence alignment based on fast Fourier transform. Nucleic Acids Research 30 (14): 3059–306. https://doi.org/10.1093/nar/gkf436
  21. Lanfear, R., Calcott, B., Kainer, D., Mayer, C. & Stamatakis, A. (2014) Selecting optimal partitioning schemes for phylogenomic datasets. BMC Evolutionary Biology 14: 1–14. https://doi.org/10.1186/1471-2148-14-82
  22. Lanfear, R., Frandsen, P.B., Wright, A.M., Senfeld, T. & Calcott, B. (2017) PartitionFinder 2: New methods for selecting partitioned models of evolution for molecular and morphological phylogenetic analyses. Molecular Biology and Evolution 34 (3): 772–773. https://doi.org/10.1093/molbev/msw260
  23. Martínez-González, C.R., Ramírez-Mendoza, R., Jiménez-Ramírez, J., Gallegos-Vázquez, C. & Luna-Vega, I. (2017) Improved method for genomic DNA extraction for Opuntia Mill. (Cactaceae). Plant Methods 13: 1−10.
  24. Montecchi, A. & Sarasini, M. (2000) Funghi ipogei d’Europa. Associazione Micologica Bresadola, Fondazione Centro Studi Micologici. Vicenza. 714 pp.
  25. Montoya, A., Hernández-Totomoch, O., Estrada-Torres, A. & Kong, A. (2003) Traditional knowledge about mushrooms in a Nahua community in the state of Tlaxcala, Mexico. Mycologia 95 (5): 793–806.
  26. Montoya, A., Kong, A., Estrada-Torres, A., Cifuentes, J. & Caballero, J. (2004) Useful wild fungi of La Malinche National Park, Mexico. Fungal Diversity 17: 115–143.
  27. Montoya, A., Torres-García, E., Kong, A., Estrada-Torres, A. & Caballero, J. (2012) Gender differences and regionalization of the cultural significance of wild mushrooms around La Malinche volcano, Tlaxcala, Mexico. Mycologia 104 (4): 826–834. https://doi.org/10.3852/11-347
  28. Müller, K., Quandt, D., Müller, J. & Neinhuis, C. (2005) PhyDE®-Phylogenetic data editor, versión 10.0. Program distributed by the authors. Available from: https://www.phyde.de (accessed: 16 February 2022).
  29. Nguyen, L.T., Schmidt, H.A., Von Haeseler, A. & Minh, B.Q. (2015) IQ-TREE: A Fast and Effective Stochastic Algorithm for Estimating Maximum-Likelihood Phylogenies. Molecular Biology and Evolution 32 (1): 268–274. https://doi.org/10.1093/molbev/msu300
  30. Payen, T., Murat, C. & Bonito, G. (2014) Truffle phylogenomics: New insights into truffle evolution and truffle life cycle. Advances in Botanical Research 70: 211–234. https://doi.org/10.1016/B978-0-12-397940-7.00007-0
  31. Pérez-Moreno, J., Mortimer, P.E., Karunarathna, S.C. & Li, H. (2021b) Global perspectives on the ecological, cultural and socioeconomic relevance of wild edible fungi. Studies in Fungi 6: 408–424. https://doi.org/10.5943/sif/6/1/31
  32. Pérez-Moreno, J., Guerin-Laguette, A., Rinaldi, A.C., Yu, F.G., Verbeken, A., Hernández-Santiago, F. & Martínez-Reyes, M. (2021a) Edible mycorrhizal fungi of the world: What is their role in forest sustainability, food security, biocultural conservation and climate change? Plants, People, Planet 3: 471–490. https://doi.org/10.1002/ppp3.10199
  33. Rambaut, A. (2018) FigTree version 1.4.4. Available from: http://tree.bio.ed.ac.uk/software/figtree/ (accessed: 1 August 2023).
  34. Rambaut, A., Drummond, A.J., Xie, D., Baele, G. & Suchard, M.A. (2018) Posterior Summarization in Bayesian Phylogenetics Using Tracer 1.7. Systematic biology 67 (5): 901–904. https://doi.org/10.1093/sysbio/syy032
  35. Reyes-López, R., Montoya, A., Kong, A., Cruz-Campuzano, E. & Caballero-Nieto, J. (2020) Folk classification of wild mushrooms from San Isidro Buensuceso, Tlaxcala, Central Mexico. Journal of Ethnobiology and Ethnomedicine 16: 53. https://doi.org/10.1186/s13002-020-00408-x
  36. Ronquist, F., Teslenko, M., Van der Mark, P., Ayres, D.L., Darling, A., Höhna, S., Larget, B., Liu, L., Suchard, M.A. & Huelsenbeck, J.P. (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across large model space. Systematic Biology 61: 539–542.
  37. Sánchez-Ledezma, J.A., Guevara-Guerrero, G., Garibay-Origel, R., Ángeles-Argáiz, R., Ávila-Rodríguez, V., Arreola-Ávila, J.G., Carrasco-Hernández, V., Borja-De la Rosa, A. & Gonzáles-García, F. (2023) Tuber caryophilum, a new truffle species growing in Carya illinoinensis orchards. Revista Mexicana de Biodiversidad 93: e934893. https://doi.org/10.22201/ib.20078706e.2022.93.4893
  38. Swofford, D.L. (2002) PAUP* Phylogenetic analysis using parsimony (*and other methods). Version 4. Sinauer Associates, Sunderland.
  39. Trappe, J.M., Molina, R., Luoma, D.L., Cázares, E., Pilz, D., Smith, J.E., Castellano, M.A., Miller, L. & Trappe, J.M. (2009) Diversity, ecology and conservation of the truffle fungi in forests of the pacific northwest. US Dept. of Agriculture, Forest Service General Technical Report, PNW-GTR-772. Oregon. 194 pp.
  40. White, T.J., Bruns, T.D., Lee, S. & Taylor, J.W. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis, M.A., Gelfand, D.H., Sninsky, J. & White, T.J. (Eds.) PCR protocols: a guide to methods and applications. Academic, San Diego, pp. 315–322. https://doi.org/10.1016/B978-0-12-372180-8.50042-1
  41. Zhang, Z., Schwartz, S., Wagner, L. & Miller, W. (2000) A greedy algorithm for aligning DNA sequences. Journal of Computational Biology 7: 203−214.