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Issue: Vol. 626 No. 3: 23 Nov. 2023
Type: Article
Published: 2023-11-23
DOI: 10.11646/phytotaxa.626.3.3
Page range: 170-180
Abstract views: 109
PDF downloaded: 89

Development of SSR markers identification system for Carex L. based on RAD sequencing

Beijing Key Laboratory of Greening Plants Breeding; Beijing Institute of Architecture; 100102; Beijing; P.R. China
National Key Laboratory of Wheat Improvement; Peking University Institute of Advanced Agricultural Sciences; Shandong Laboratory of Advanced Agricultural Sciences in Weifang; 261325; Shandong; P.R. China
Beijing Key Laboratory of Greening Plants Breeding; Beijing Institute of Architecture; 100102; Beijing; P.R. China
Beijing Key Laboratory of Greening Plants Breeding; Beijing Institute of Architecture; 100102; Beijing; P.R. China
Beijing Key Laboratory of Greening Plants Breeding; Beijing Institute of Architecture; 100102; Beijing; P.R. China
Beijing Key Laboratory of Greening Plants Breeding; Beijing Institute of Architecture; 100102; Beijing; P.R. China
Beijing Key Laboratory of Greening Plants Breeding; Beijing Institute of Architecture; 100102; Beijing; P.R. China
College of Landscape Architecture and Tourism; Hebei Agricultural University; 071000; Baoding; Hebei; P.R. China
Beijing Radiation Center; Beijing Academy of Science and Technology; 102300; Beijing; P.R. China
Science and technology exploitation and examination base of Mentougou District; 102300; Beijing; P.R. China
Beijing Key Laboratory of Greening Plants Breeding; Beijing Institute of Architecture; 100102; Beijing; P.R. China
Beijing Key Laboratory of Greening Plants Breeding; Beijing Institute of Architecture; 100102; Beijing; P.R. China
Beijing Key Laboratory of Greening Plants Breeding; Beijing Institute of Architecture; 100102; Beijing; P.R. China
Beijing Key Laboratory of Greening Plants Breeding; Beijing Institute of Architecture; 100102; Beijing; P.R. China
Beijing Key Laboratory of Greening Plants Breeding; Beijing Institute of Architecture; 100102; Beijing; P.R. China
Beijing Key Laboratory of Greening Plants Breeding; Beijing Institute of Architecture; 100102; Beijing; P.R. China
Beijing Key Laboratory of Greening Plants Breeding; Beijing Institute of Architecture; 100102; Beijing; P.R. China
Monocots Carex L. Restriction site-associated DNA sequencing SNP SSR Molecular identification

Abstract

In this study, we designed and evaluated an efficient set of simple sequence repeat (SSR) markers from restriction site-associated DNA (RAD) sequencing data of four widely utilized Carex L. accession across Northern China, especially in Beijing. Based on their genomic sequencing data, we developed 400 SSR markers and evaluated their amplification specificities among which a total of 17 SSR markers were identified as a core set of molecular markers for efficient assessment of diverse Carex L. accessions. Using this molecular identification system, we classified 26 Carex L. accessions into 3 genetically distinct groups. The establishment of a molecular identification system based on a core set of 17 SSR markers provides an essential basis for evaluating genetic relationships among Carex L. accessions along with a wealth of genetic resources for marker-assisted selection and breeding of Carex L..

 

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