Skip to main content Skip to main navigation menu Skip to site footer
Type: Article
Published: 2023-11-15
Page range: 67-72
Abstract views: 119
PDF downloaded: 8

Trichoderma orarium (Hypocreales): a new species from Taiwan

Bioresource Collection and Research Center (BCRC); Food Industry Research & Development Institute (FIRDI); No. 331; Shih-Pin Road; Hsinchu TW-300193; Taiwan; R.O.C.,Bioresource Collection and Research Center (BCRC); Food Industry Research & Development Institute (FIRDI); No. 331; Shih-Pin Road; Hsinchu TW-300193; Taiwan; R.O.C.
Department of Plant Pathology and Microbiology; National Taiwan University; No. 1; Sec. 4; Roosevelt Road; Taipei TW-106319; Taiwan; R.O.C.
Bioresource Collection and Research Center (BCRC); Food Industry Research & Development Institute (FIRDI); No. 331; Shih-Pin Road; Hsinchu TW-300193; Taiwan; R.O.C.
Bioresource Collection and Research Center (BCRC); Food Industry Research & Development Institute (FIRDI); No. 331; Shih-Pin Road; Hsinchu TW-300193; Taiwan; R.O.C.
Trichoderma Harzianum clade phylogenetic analyses Morphology Fungi

Abstract

Trichoderma spp. are widely distributed worldwide. Members of Trichoderma have been shown to play a significant role in the biocontrol of plant disease, as well as the industrial production of enzymes and antibiotics, and as a mycoparasite of edible fungi. In this study, a new species, Trichoderma orarium, is introduced and illustrated. This new species was isolated from coastal soil in Northeastern Taiwan and identified based on morphological characteristics and multi-gene sequence analyses of three loci (ITS, tef1, and rpb2). The phylogenetic analyses revealed that T. orarium belongs to the Harzianum clade. Distinctions between the new species and its close relatives are discussed in detail.

 

References

  1. Barrera, V.A., Iannone, L., Romero, A.I. & Chaverri, P. (2021) Expanding the Trichoderma harzianum species complex: three new species from Argentine natural and cultivated ecosystems. Mycologia 113: 1136–1155. https://doi.org/10.1080/00275514.2021.1947641
  2. Cai, F. & Druzhinina, I.S. (2021) In honor of John Bissett: authoritative guidelines on molecular identification of Trichoderma. Fungal Diversity 107: 1–69. https://doi.org/10.1007/s13225-020-00464-4
  3. Cao, Z.-J., Qin, W.-T., Zhao, J., Liu, Y., Wang, S.-X. & Zheng, S.-Y. (2022) Three new Trichoderma species in Harzianum clade associated with the contaminated substrates of edible fungi. Journal of Fungi 8: 1154. https://doi.org/10.3390/jof8111154
  4. Carbone, I. & Kohn, L.M. (1999) A method for designing primer sets for speciation studies in filamentous ascomycetes. Mycologia 91: 553–556. https://doi.org/10.1080/00275514.1999.12061051
  5. Dou, K., Lu, Z., Wu, Q., Ni, M., Yu, C., Wang, M., Li, Y., Wang, X., Xie, H., Chen, J. & Zhang, C. (2020) MIST: a multilocus identification system for Trichoderma. Applied and Environmental Microbiology 86: e01532–20. https://doi.org/10.1128/AEM.01532-20
  6. Gardes, M. & Bruns, T.D. (1993) ITS primers with enhanced specificity for basidiomycetes — application to the identification of mycorrhizae and rusts. Molecular Ecology 2: 113–118. https://doi.org/10.1111/j.1365-294X.1993.tb00005.x
  7. Gu, X., Wang, R., Sun, Q., Wu, B. & Sun, J.-Z. (2020) Four new species of Trichoderma in the Harzianum clade from northern China. MycoKeys 73: 109–132. https://doi.org/10.3897/mycokeys.73.51424
  8. Jaklitsch, W.M., Komon, M., Kubicek, C.P. & Druzhinina, I.S. (2005) Hypocrea voglmayrii sp. nov. from the Austrian Alps represents a new phylogenetic clade in Hypocrea/Trichoderma. Mycologia 97: 1365–1378. https://doi.org/10.1080/15572536.2006.11832743
  9. Liu, Y.J., Whelen, S. & Hall, B.D. (1999) Phylogenetic relationships among ascomycetes: evidence from an RNA polymerse II subunit. Molecular Biology and Evolution 16: 1799–1808. https://doi.org/10.1080/15572536.2008.11832477
  10. Persoon, C.H. (1794) Neuer Versuch einer systematischen Eintheilung der Schwämme. In: Römer, J.J. (ed.) Neues Magazin für die Botanik in ihrem ganzen Umfange. Zürich, pp. 63–128. [https://www.e-rara.ch/zut/doi/10.3931/e-rara-37279]
  11. Phookamsak, R., Hyde, K.D., Jeewon, R., Bhat, D.J., Jones, E.B.G., Maharachchikumbura, S.S.N., Raspe, O., Karunarathna, S.C., Wanasinghe, D.N. & Hongsanan, S. (2019) Fungal diversity notes 929–1035: taxonomic and phylogenetic contributions on genera and species of fungi. Fungal Diversity 95: 1–273. https://doi.org/10.1007/s13225-019-00421-w
  12. Prameeladevi, T., Kamil, D., Sudeep Toppo, R. & Choudhary, S. (2021a) Trichoderma dumbbelliforme sp. nov. an undescribed fungus of order Hypocreales from India. Phytotaxa 520: 285–295. https://doi.org/10.11646/phytotaxa.520.3.8
  13. Prameeladevi, T., Narayanasamy, P., Kamil, D. & Choudhary, S. (2021b) Polyphasic taxonomy of Indian Trichoderma species. Phytotaxa 502: 1–27. https://doi.org/10.11646/phytotaxa.502.1
  14. Sousa, T.F., Reça, B.N.P.V., Castro, G.S., da Silva, I.J.S., Caniato, F.F., Júnior, M.B.de A., Yamagishi, M.E.B., Koolen, H.H.F., Bataglion, G.A., Hanada, R.E. & da Silva, G.F. (2023) Trichoderma agriamazonicum sp. nov. (Hypocreaceae), a new ally in the control of phytopathogens. Microbiological Research 275: 127469. https://doi.org/10.1016/j.micres.2023.127469
  15. Tamura, K., Stecher, G. & Kumar, S. (2021) MEGA11: Molecular evolutionary genetics analysis version 11. Molecular Biology and Evolution 38: 3022–3027. https://doi.org/10.1093/molbev/msab120
  16. White, T.J., Bruns, T.D., Lee, S.B. & Taylor, J.W. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis, M.A., Gelfand, D.H., Sninsky, J.J. & White, T.J. (Eds.) PCR Protocols — a guide to methods and applications. Academic Press, San Diego, pp. 315–322. https://doi.org/10.1016/B978-0-12-372180-8.50042-1
  17. Zheng, H., Qiao, M., Lv, Y., Du, X., Zhang, K.Q. & Yu, Z. (2021) New species of Trichoderma isolated as endophytes and saprobes from southwest China. Journal of Fungi 7: 467. [https://www.mdpi.com/2309-608X/7/6/467]