Skip to main content Skip to main navigation menu Skip to site footer
Type: Article
Published: 2023-11-07
Page range: 66-76
Abstract views: 93
PDF downloaded: 11

Addition to Pseudoplagiostomataceae: Pseudoplagiostoma inthanonense sp. nov. from Doi Inthanon National Park, Northern Thailand

School of Science; Mae Fah Luang University; Chiang Rai; 57100; Thailand; Center of Excellence in Fungal Research; Mae Fah Luang University; Chiang Rai; 57100; Thailand
Center of Excellence in Fungal Research; Mae Fah Luang University; Chiang Rai; 57100; Thaila; School of Biological Sciences and Institute of Microbiology; Seoul National University; Seoul; 08826; Republic of Korea
School of Science; Mae Fah Luang University; Chiang Rai; 57100; Thailand; Center of Excellence in Fungal Research; Mae Fah Luang University; Chiang Rai; 57100; Thailand
1 new species plant litter fungi coelomycetes lifestyle saprobic Fungi

Abstract

Fungi are dominant microorganisms in plant litter, especially in pristine environments. This study collected leaf litter of broadleaf species within Doi Inthanon National Park, Chiang Mai, Thailand. Phylogenetic analyses utilizing DNA sequence data from ITS, LSU, tef1-α, rpb2, and tub2 regions and morphological characteristics revealed a new species, Pseudoplagiostoma inthanonense (Pseudoplagiostomataceae). The phylogeny and morphology of this novel species are thoroughly described and supported with detailed illustrations. Pseudoplagiostoma inthanonense is characterized by the shape and size of the conidiogenous cell and spore size. The discovery of P. inthanonense confirms that Pseudoplagiostoma consists of pathogenic, endophytic, and saprobic life forms while creating new research opportunities on potential lifestyle changes of Pseudoplagiostoma species.

References

  1. Bezerra, J., Pádua, A., Oliveira, T., Paiva, L., Guarnaccia, V., Fan, X. & Souza-Motta, C. (2019) Pseudoplagiostoma myracrodruonis (Pseudoplagiostomataceae, Diaporthales): a new endophytic species from Brazil. Mycological Progress 18: 1329–1339. https://doi.org/10.1007/s11557-019-01531-0
  2. Carbone, I. & Kohn, L.M. (1999) A method for designing primer sets for speciation studies in filamentous ascomycetes. Mycologia 91: 553–556. https://doi.org/10.1080/00275514.1999.12061051
  3. Chaiwan, N. (2021) https://gmsmicrofungi.org: an online database providing updated information of microfungi in the Greater Mekong Subregion. Mycosphere 12: 1513–1526. https://doi.org/10.5943/mycosphere/12/1/19
  4. Cheewangkoon, R., Groenewald, J.Z., Verkley, G., Hyde, K., Wingfield, M., Gryzenhout, M., Summerell, B., Denman, S., Toanun, C. & Crous, P. (2010) Re-evaluation of Cryptosporiopsis eucalypti and Cryptosporiopsis-like species occurring on Eucalyptus leaves. Fungal diversity 44: 89–105. https://doi.org/10.1007/s13225-010-0041-5
  5. Chethana, K.T., Manawasinghe, I.S., Hurdeal, V.G., Bhunjun, C.S., Appadoo, M.A., Gentekaki, E., Raspé, O., Promputtha, I. & Hyde, K.D. (2021) What are fungal species and how to delineate them? Fungal Diversity 109: 1–25. https://doi.org/10.1007/s13225-021-00483-9
  6. Chomnunti, P., Hongsanan, S., Aguirre-Hudson, B., Tian, Q., Persoh, D., Dhami, M., Xu, J., Liu, X., Stadler, M., Hyde, K. & Alias, A. (2014) The sooty moulds. Fungal diversity 66: 1–36. https://doi.org/10.1007/s13225-014-0278-5
  7. Crous, P., Summerell, B., Shivas, R., Burgess, T., Decock, C., Dreyer, L., Granke, L., Guest, D., Hardy, G., Hausbeck, M., Hüberli, D., Jung, T., Koukol, O., Lennox, C.L., Liew, E.C.Y., Lombard, L., McTaggart, A., Pryke, J., Roets, F. & Groenewald, J.Z. (2012) Fungal Planet description sheets: 107–127. Persoonia 28: 138–82. https://doi.org/10.3767/003158512X652633
  8. Crous, P.W., Luangsa-ard, J.J., Wingfield, M.J., Carnegie, A.J., Hernández-Restrepo, M., Lombard, L., Roux, J., Barreto, R.W., Baseia, I.G., Cano-Lira, J.F., Martín, M.P., Morozova, O.V., Stchigel, A.M., Summerell, B.A., Brandrud, T.E., Dima, B., García, D., Giraldo, A., Guarro, J., Gusmão, L.F.P., Khamsuntorn, P., Noordeloos, M.E., Nuankaew, S., Pinruan, U., Rodríguez-Andrade, E., Souza-Motta, C.M., Thangavel, R., van Iperen, A.L., Abreu, V.P., Accioly, T., Alves, J.L., Andrade, J.P., Bahram, M., Baral, H.-O., Barbier, E., Barnes, C.W., Bendiksen, E., Bernard, E., Bezerra, J.D.P., Bezerra, J.L., Bizio, E., Blair, J.E., Bulyonkova, T.M., Cabral, T.S., Caiafa, M.V., Cantillo, T., Colmán, A.A., Conceição, L.B., Cruz, S., Cunha, A.O.B., Darveaux, B.A., da Silva, A.L., da Silva, G.A., da Silva, G.M., da Silva, R.M.F., de Oliveira, R.J.V., Oliveira, R.L., De Souza, J.T., Dueñas, M., Evans, H.C., Epifani, F., Felipe, M.T.C., Fernández-López, J., Ferreira, B.W., Figueiredo, C.N., Filippova, N.V., Flores, J.A., Gené, J., Ghorbani, G., Gibertoni, T.B., Glushakova, A.M., Healy, R., Huhndorf, S.M., Iturrieta-González, I., Javan-Nikkhah, M., Juciano, R.F., Jurjević, Ž., Kachalkin, A.V., Keochanpheng, K., Krisai-Greilhuber, I., Li, Y.-C., Lima, A.A., Machado, A.R., Madrid, H., Magalhães, O.M.C., Marbach, P.A.S., Melanda, G.C.S., Miller, A.N., Mongkolsamrit, S., Nascimento, R.P., Oliveira, T.G.L., Ordoñez, M.E., Orzes, R., Palma, M.A., Pearce, C.J., Pereira, O.L., Perrone, G., Peterson, S.W., Pham, T.H.G., Piontelli, E., Pordel, A., Quijada, L., Raja, H.A., Rosas de Paz, E., Ryvarden, L., Saitta, A., Salcedo, S.S., Sandoval-Denis, M., Santos, T.A.B., Seifert, K.A., Silva, B.D.B., Smith, M.E., Soares, A.M., Sommai, S., Sousa, J.O., Suetrong, S., Susca, A., Tedersoo, L., Telleria, M.T., Thanakitpipattana, D., Valenzuela-Lopez, N., Visagie, C.M., Zapata, M. & Groenewald, J.Z. (2018) Fungal Planet description sheets: 785–867. Persoonia : Molecular Phylogeny and Evolution of Fungi 41: 238–417. https://doi.org/10.3767/persoonia.2018.41.12
  9. Glass, N.L. & Donaldson, G.C. (1995) Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. Applied and environmental microbiology 61: 1323–1330. https://doi.org/10.1128/aem.61.4.1323-1330.1995
  10. Hall, T.A. (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic acids symposium series. Oxford, pp. 95–98.
  11. Hoang, D.T., Chernomor, O., Von Haeseler, A., Minh, B.Q. & Vinh, L.S. (2018) UFBoot2: improving the ultrafast bootstrap approximation. Molecular biology and evolution 35: 518–522. https://doi.org/10.1093/molbev/msx281
  12. Hyde, K.D., Norphanphoun, C., Chen, J., Dissanayake, A.J., Doilom, M., Hongsanan, S., Jayawardena, R.S., Jeewon, R., Perera, R.H. & Thongbai, B. (2018) Thailand’s amazing diversity: up to 96% of fungi in northern Thailand may be novel. Fungal diversity 93: 215–239. https://doi.org/10.1007/s13225-018-0415-7
  13. Kalyaanamoorthy, S., Minh, B.Q., Wong, T.K., Von Haeseler, A. & Jermiin, L.S. (2017) ModelFinder: fast model selection for accurate phylogenetic estimates. Nature methods 14: 587–589. https://doi.org/10.1038/nmeth.4285
  14. Katoh, K., Rozewicki, J. & Yamada, K.D. (2019) MAFFT online service: multiple sequence alignment, interactive sequence choice and visualization. Briefings in bioinformatics 20: 1160–1166. https://doi.org/10.1093/bib/bbx108
  15. Kumla, J., Suwannarach, N., Wannathes, N. & Lumyong, S. (2022) Survey of Volvariella (Agaricales, Basidiomycota) including two new species, V. neovolvacea and V. thailandensis, from Northern Thailand. Diversity 14: 161. https://doi.org/10.3390/d14030161
  16. Liu, Y.J., Whelen, S. & Hall, B.D. (1999) Phylogenetic relationships among ascomycetes: evidence from an RNA polymerse II subunit. Molecular biology and evolution 16: 1799–1808. https://doi.org/10.1093/oxfordjournals.molbev.a026092
  17. Lueangpraplut, S., Unartngam, A. & Unartngam, J. (2013) Molecular identification of Pseudoplagiostoma eucalypti causing leaf spot and shoot blight diseases on eucalyptus in Thailand based on ITS rDNA sequence. Journal of Agricultural Technology 9: 165–175.
  18. Maharachchikumbura, S.S., Chen, Y., Ariyawansa, H.A., Hyde, K.D., Haelewaters, D., Perera, R.H., Samarakoon, M.C., Wanasinghe, D.N., Bustamante, D.E. & Liu, J.-K. (2021) Integrative approaches for species delimitation in Ascomycota. Fungal Diversity 109: 155–179. https://doi.org/10.1007/s13225-021-00486-6
  19. Miller, M.A., Pfeiffer, W. & Schwartz, T. (2010) Creating the CIPRES Science Gateway for inference of large phylogenetic trees. 2010 gateway computing environments workshop (GCE). Ieee, pp. 1–8. https://doi.org/10.1109/GCE.2010.5676129
  20. Mu, T.C., Zhang, Z., Liu, R.Y., Li, Z., Zhang, X.G. & Xia, J. (2022) Morphological and molecular identification of Pseudoplagiostoma castaneae sp. nov. (Pseudoplagiostomataceae, Diaporthales) in Shandong Province, China. Nova Hedwigia 114: 171–180. https://doi.org/10.1127/nova_hedwigia/2022/0666
  21. O’Donnell, K., Kistler, H.C., Cigelnik, E. & Ploetz, R.C. (1998) Multiple evolutionary origins of the fungus causing Panama disease of banana: concordant evidence from nuclear and mitochondrial gene genealogies. Proceedings of the National Academy of Sciences 95: 2044–2049. https://doi.org/10.1073/pnas.95.5.2044
  22. Phookamsak, R., Hyde, K.D., Jeewon, R., Bhat, D.J., Jones, E.B.G., Maharachchikumbura, S.S.N., Raspé, O., Karunarathna, S.C., Wanasinghe, D.N., Hongsanan, S., Doilom, M., Tennakoon, D.S., Machado, A.R., Firmino, A.L., Ghosh, A., Karunarathna, A., Mešić, A., Dutta, A.K., Thongbai, B., Devadatha, B., Norphanphoun, C., Senwanna, C., Wei, D., Pem, D., Ackah, F.K., Wang, G.-N., Jiang, H.B., Madrid, H., Lee, H.B., Goonasekara, I.D., Manawasinghe, I.S., Kušan, I., Cano, J., Gené, J., Li, J., Das, K., Acharya, K., Raj, K.N.A., Latha, K.P.D., Chethana, K.W.T., He, M.-Q., Dueñas, M., Jadan, M., Martín, M.P., Samarakoon, M.C., Dayarathne, M.C., Raza, M., Park, M.S., Telleria, M.T., Chaiwan, N., Matočec, N., Silva, N.I. de, Pereira, O.L., Singh, P.N., Manimohan, P., Uniyal, P., Shang, Q.J., Bhatt, R.P., Perera, R.H., Alvarenga, R.L.M., Prata, S.N., Singh, S.K., Vadthanarat, S., Oh, S.Y., Huang, S.K., Rana, S., Konta, S., Paloi, S., Jayasiri, S.C., Jeon, S.J., Mehmood, T., Gibertoni, T.B., Nguyen, T.T.T., Singh, U., Thiyagaraja, V., Sarma, V.V., Dong, W., Yu, X.D., Lu, Y.Z., Lim, Y.W., Chen, Y., Tkalčec, Z., Zhang, Z.F., Luo, Z.L., Daranagama, D.A., Thambugala, K.M., Tibpromma, S., Camporesi, E., Bulgakov, T.S., Dissanayake, A.J., Senanayake, I.C., Dai, D.Q., Tang, L.Z., Khan, S., Zhang, H., Promputtha, I., Cai, L., Chomnunti, P., Zhao, R.L., Lumyong, S., Boonmee, S., Wen, T.-C., Mortimer, P.E. & Xu, J. (2019) Fungal diversity notes 929–1035: taxonomic and phylogenetic contributions on genera and species of fungi. Fungal diversity 95: 1–273. https://doi.org/10.1007/s13225-019-00421-w
  23. Phookamsak, R., Jiang, H.B., Suwannarach, N., Lumyong, S., Xu, J., Xu, S., Liao, C. & Chomnunti, P. (2022) Bambusicolous Fungi in Pleosporales: Introducing Four Novel Taxa and a New Habitat Record for Anastomitrabeculia didymospora. Journal of Fungi 8: 630. https://doi.org/10.3390/jof8060630
  24. Priyashantha, A.H., Dai, D.Q., Bhat, D.J., Stephenson, S.L., Promputtha, I., Kaushik, P., Tibpromma, S. & Karunarathna, S.C. (2023) Plant-Fungi Interactions: Where It Goes? Biology 12: 809. https://doi.org/10.3390/biology12060809
  25. Rai, M. & Agarkar, G. (2016) Plant-fungal interactions: what triggers the fungi to switch among lifestyles? Critical Reviews in Microbiology 42: 428–438. https://doi.org/10.3109/1040841X.2014.958052
  26. Rehner, S.A. & Samuels, G.J. (1994) Taxonomy and phylogeny of Gliocladium analysed from nuclear large subunit ribosomal DNA sequences. Mycological Research 98: 625–634. https://doi.org/10.1016/S0953-7562(09)80409-7
  27. Sanchez, R., Serra, F., Tárraga, J., Medina, I., Carbonell, J., Pulido, L., De María, A., Capella-Gutíerrez, S., Huerta-Cepas, J., Gabaldon, T., Dopazo, J. & Dopazo, H. (2011) Phylemon 2.0: a suite of web-tools for molecular evolution, phylogenetics, phylogenomics and hypotheses testing. Nucleic acids research 39: W470–W474. https://doi.org/10.1093/nar/gkr408
  28. Senanayake, I., Rathnayaka, A., Sandamali, D., Calabon, M., Gentekaki, E., Lee, H., Pem, D., Dissanayake, L., Wijesinghe, S., Bundhun, D., TTT, N., Goonasekara, I., Abeywickrama, P., Jayawardena, R., Wanasinghe, D., Jeewon, R., Bhat, D.J., Mm, X., Bhunjun, C.S. & Hurdeal, V. (2020) Morphological approaches in studying fungi: collection, examination, isolation, sporulation and preservation. mycosphere 11: 2678–2754. https://doi.org/10.5943/mycosphere/11/1/20
  29. Staden, R., Beal, K. & Bonfield, J.K. (1998) The Staden Package, Computer Methods in Molecular Biology, Eds, Stephen, Misener and Steve, Krawetz.
  30. Suwannarach, N., Kumla, J. & Lumyong, S. (2016) Pseudoplagiostoma dipterocarpi sp. nov., a new endophytic fungus from Thailand. Mycoscience 57 (2): 118–122. https://doi.org/10.1016/j.myc.2015.12.002
  31. Tang, X., Goonasekara, I., Jayawardena, R., Jiang, H.-B., Li, J., Hyde, K. & Kang, J. (2020) Arthrinium bambusicola (Fungi, Sordariomycetes), a new species from Schizostachyum brachycladum in northern Thailand. Biodiversity Data Journal 8: e58755. https://doi.org/10.3897/BDJ.8.e58755
  32. Tang, X., Jayawardena, R., Stephenson, S. & Kang, J. (2022) A new species Pseudoplagiostoma dipterocarpicola (Pseudoplagiostomataceae, Diaporthales) found in northern Thailand on members of the Dipterocarpaceae. Phytotaxa 543 (4): 233–243. https://doi.org/10.11646/phytotaxa.543.4.3
  33. Teejuntuk, S., Sahunalu, P., Sakurai, K. & Sungpalee, W. (2003) Forest Structure and Tree Species Diversity along an Altitudinal Gradient in Doi Inthanon National Park, Northern Thailand. Tropics 12: 85–102. https://doi.org/10.3759/tropics.12.85
  34. Trifinopoulos, J., Nguyen, L.T., von Haeseler, A. & Minh, B.Q. (2016) W-IQ-TREE: a fast online phylogenetic tool for maximum likelihood analysis. Nucleic acids research 44: W232–W235. https://doi.org/10.1093/nar/gkw256
  35. Tsui, K.M., Hyde, K.D. & Hodgkiss, I.J. (2003) Methods for investigating the biodiversity and distribution of freshwater ascomycetes and anamorphic fungi on submerged wood.
  36. Vilgalys, R. & Hester, M. (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. Journal of bacteriology 172: 4238–4246. https://doi.org/10.1128/jb.172.8.4238-4246.1990
  37. Wei, D.P., Wanasinghe, D.N., Gentekaki, E., Thiyagaraja, V., Lumyong, S. & Hyde, K.D. (2021) Morphological and phylogenetic appraisal of novel and extant taxa of Stictidaceae from Northern Thailand. Journal of Fungi 7: 880. https://doi.org/10.3390/jof7100880
  38. White, T.J., Bruns, T., Lee, S. & Taylor, J. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR protocols: a guide to methods and applications 18: 315–322. https://doi.org/10.1016/B978-0-12-372180-8.50042-1
  39. Wijayawardene, N., Hyde, K., Anand, G., Ls, D., Tang, L.-Z. & Dai, D.-Q. (2021) Towards incorporating asexually reproducing fungi in the natural classification and notes for pleomorphic genera. mycosphere 12: 238–405. https://doi.org/10.5943/mycosphere/12/1/4
  40. Wijayawardene, N., Hyde, K., Dai, D., Sánchez-García, M., Goto, B., Saxena, R., Erdoðdu, M., Selçuk, F., Rajeshkumar, K., Aptroot, A., Błaszkowski, J., Boonyuen, N., Da Silva, G., De Souza, F., Dong, W., Ertz, D., Haelewaters, D., Jones, E., Karunarathna, S., Kirk, P., Kukwa, M., Kumla, J., Leontyev, D., Lumbsch, H., Maharachchikumbura, S., Marguno, F., Martínez-Rodríguez, P., Mešić, A., Monteiro, J., Oehl, F., Pawłowska, J., Pem, D., Pfliegler, W., Phillips, A., Pošta, A., He, M., Li, J., Raza, M., Sruthi, O., Suetrong, S., Suwannarach, N., Tedersoo, L., Thiyagaraja, V., Tibpromma, S., Tkalčec, Z., Tokarev, Y., Wanasinghe, D., Wijesundara, D., Wimalaseana, S., Madrid, H., Zhang, G., Gao, Y., Sánchez-Castro, I., Tang, L., Stadler, M., Yurkov, A. & Thines, M. (2022) Outline of Fungi and fungus-like taxa-2021. Mycosphere 13: 53–453. https://doi.org/10.5943/mycosphere/13/1/2
  41. Zhang, Z., Liu, X., Tao, M., Liu, X., Xia, J., Zhang, X. & Meng, Z. (2023) Taxonomy, Phylogeny, Divergence Time Estimation, and Biogeography of the Family Pseudoplagiostomataceae (Ascomycota, Diaporthales). Journal of Fungi 9: 82. https://doi.org/10.3390/jof9010082