Skip to main content Skip to main navigation menu Skip to site footer
Responsive image
Issue: Vol. 601 No. 3: 11 July 2023
Type: Article
Published: 2023-07-11
DOI: 10.11646/phytotaxa.601.3.2
Page range: 212–222
Abstract views: 162
PDF downloaded: 15

A new species of Helvella (Helvellaceae, Pezizomycetes) within the H. macropus group from China

Key Laboratory of Forest Disaster Warning and Control in Yunnan Province, College of Biodiversity Conservation, Southwest Forestry University, Kunming, Yunnan, 650224, China, Key Laboratory for Plant Diversity and Biogeography of East Asia, Yunnan Key Laboratory of Fungal Diversity and Green Development, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan 650201, China
Key Laboratory for Plant Diversity and Biogeography of East Asia, Yunnan Key Laboratory of Fungal Diversity and Green Development, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan 650201, China
Key Laboratory for Plant Diversity and Biogeography of East Asia, Yunnan Key Laboratory of Fungal Diversity and Green Development, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan 650201, China
Key Laboratory of Forest Disaster Warning and Control in Yunnan Province, College of Biodiversity Conservation, Southwest Forestry University, Kunming, Yunnan, 650224, China
Key Laboratory for Plant Diversity and Biogeography of East Asia, Yunnan Key Laboratory of Fungal Diversity and Green Development, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan 650201, China
1 new taxon complex group endemic saddle fungi species diversity Fungi

Abstract

The brown cupulate saddle fungi with villus surface in China, have long been named as Helvella macropus, a well-known European and North American species. In this study, Helvella fruiting bodies from Austria, China (the Tibetan Plateau), and Germany were examined. Phylogenetic analyses of combined LSU, TEF and HSP90 sequence data showed that the Chinese H. macropus-like samples represent two phylogenetic species, one of which is H. macropus s.s., and the other is H. guttata, which is introduced as new species here. Helvella guttata and H. macropus are described and illustrated in detail and compared with their similar species. In addition, a key to the morphologically recognizable taxa of the H. macropus complex is provided.

References

  1. Abbott, S.P. & Currah, R.S. (1997) The Hevellaceae: systematic revision and occurrence in northern and northwestern North America. Mycotaxon 62: 1–125.

  2. Ariyawansa, H., Hyde, K.D., Jayasiri, S., Buyck, B., Chethana, K.W.T., Dai, D., Dai, Y.C., Daranagama, D., Jayawardena, R., Lücking, R., Ghobad-Nejhad, M., Niskanen, T., Thambugala, K., Voigt, K., Zhao, R., Li, G.J., Doilom, M., Boonmee, S., Yang, Z., Cai, Q., Cui, Y.Y., Bahkali, A., Chen, J., Cui, B., Chen, J., Dayarathne, M., Dissanayake, A., Ekanayaka, A., Hashimoto, A., Hongsanan, S., Jones, E.B.G., Larsson, E., Li, W., Li, Q.R., Liu, J., Luo, Z., Maharachchikumbura, S.N., Mapook, A., McKenzie, E.C., Norphanphoun, C., Konta, S., Pang, K., Perera, R., Phookamsak, R., Phukhamsakda, C., Pinruan, U., Randrianjohany, E., Singtripop, C., Tanaka, K., Tian, C., Tibpromma, S., Abdel-Wahab, M., Wanasinghe, D., Wijayawardene, N., Zhang, J.F., Zhang, H., Abdel-Aziz, F., Wedin, M., Westberg, M., Ammirati, J., Bulgakov, T., Lima, D., Callaghan, T., Callac, P., Chang, C.H., Coca, L., Dal-Forno, M., Dollhofer, V., Fliegerová, K., Greiner, K., Griffith, G., Ho, H .M., Hofstetter, V., Jeewon, R., Kang, J., Wen, T.C., Kirk, P., Kytövuori, I., Lawrey, J., Xing, J., Li, H., Liu, Z., Liu, X., Liimatainen, K., Lumbsch, H.T., Matsumura, M., Moncada, B., Nuankaew, S., Parnmen, S., de Azevedo Santiago, A.L.C.M., Sommai, S., Song, Y., de Souza, C.F., de Souza-Motta, C., Su, H., Suetrong, S., Wang, Y., Wei, S.F., Wen, T., Yuan, H., Zhou, L., Réblová, M., Fournier, J., Camporesi, E., Luangsa-ard, J.J., Tasanathai, K., Khonsanit, A., Thanakitpipattana, D., Somrithipol, S., Diederich, P., Millanes, A., Common, R., Stadler, M., Yan, J., Li, X., Lee, H., Nguyen, T.T., Lee, H., Battistin, E., Marsico, O., Vizzini, A., Vila, J., Ercole, E., Eberhardt, U., Simonini, G., Wen, H.A. & Chen, X.H. (2015) Fungal diversity notes 111–252—taxonomic and phylogenetic contributions to fungal taxa. Fungal Diversity 75: 1–248. http://dx.doi.org/10.1007/s13225-015-0346-5

  3. Dissing, H. (1966) The genus Helvella in Europe with special emphasis on the species found in Norden. Dansk Botanisk Arkiv 25: 1–172.

  4. Guindon, S., Dufayard, J.F., Lefort, V., Anisimova, M., Hordijk, W. & Gascuel, O. (2010) New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0. Systematic Biology 59: 307–321. https://doi.org/10.1093/sysbio/syq010

  5. Häffner, J. (1987) Die Gattung Helvella: Morphologie und Taxonomie. Beihefte zur Zeitschrift für Mykologie 7: 1–165.

  6. Hall, T.A. (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT[C]. Nucleic acids symposium series 41: 95–98. [London: Information Retrieval Ltd., c1979-c2000.]

  7. Hansen, K., Perry, B.A., Dranginis, A.W. & Pfister, D.H. (2013) A phylogeny of the highly diverse cup-fungus family Pyronemataceae (Pezizomycetes, Ascomycota) clarifies relationships and evolution of selected life history traits. Molecular Phylogenetics and Evolution 67: 311–335. https://doi.org/10.1016/j.ympev.2013.01.014

  8. Hansen, K., Schumacher, T., Skrede, I., Huhtinen, S. & Wang, X.H. (2019) Pindara revisited–evolution and generic limits in Helvellaceae. Persoonia - Molecular Phylogeny and Evolution of Fungi 42: 186–204. https://doi.org/10.3767/persoonia.2019.42.07

  9. Hoang, D.T., Chernomor, O., Von Haeseler, A., Minh, B.Q. & Vinh, L.S. (2018) UFBoot2: improving the ultrafast bootstrap approximation. Molecular Biology and Evolution 35: 518–522. https://doi.org/10.1093/molbev/msx281

  10. Hu, Y., Karunarathna, S.C., Li, H., Galappaththi, M.C., Zhao, C.L., Kakumyan, P. & Mortimer, P.E. (2022) The impact of drying temperature on basidiospore size. Diversity 14 (4): 239.

  11. Hwang, J., Zhao, Q., Yang, Z.L., Wang, Z. & Townsend, J.P. (2015) Solving the ecological puzzle of mycorrhizal associations using data from annotated collections and environmental samples – an example of saddle fungi. Environmental Microbiology Reports 7: 658–667. https://doi.org/10.1111/1758-2229.12303

  12. Imai, S. (1932) Contribution to the Knowledge of the Classification of Helvellaceae. Shokubutsugaku Zasshi 46: 172–175, 359. https://doi.org/10.15281/jplantres1887.46.172

  13. Index Fungorum (2023) Available from: https://www.indexfungorum.org/names/Names.asp (accessed 10 July 2023)

  14. Jayasiri, S.C., Hyde, K.D., Ariyawansa, H.A., Bhat, J., Buyck, B., Cai, L., Dai, Y.C., Abd-Elsalam, K.A., Ertz, D., Hidayat, I., Jeewon, R., Jones, E.B.G., Bahkali, A.H., Karunarathna, S.C., Liu, J.K., Luangsa-ard, J.J., Lumbsch, H.T., Maharachchikumbura, S.S.N., McKenzie, E.H.C., Moncalvo, J.M., Ghobad-Nejhad, M., Nilsson, H., Pang, K.L., Pereira, O.L., Phillips, A.J.L. Raspé, O., Rollins, A.W., Romero, A.I., Etayo, J., Selçuk, F., Stephenson, S.L., Suetrong, S., Taylor, J.E., Tsui, C.K.M., Vizzini, A., Abdel-Wahab, M.A., Wen, T.C., Boonmee, S., Dai, D.Q., Daranagama, D.A., Dissanayake, A.J., Ekanayaka, A.H., Fryar, S.C., Hongsanan, S., Jayawardena, R.S., Li, W.J., Perera, R.H., Phookamsak, R., de Silva, N.I., Thambugala, K.M., Tian, Q., Wijayawardene, N.N., Zhao, R.L., Qi Zhao, Q., Kang, J.C. & Promputtha, I. (2015) The Faces of Fungi database: fungal names linked with morphology, phylogeny and human impacts. Fungal diversity 74: 3–18. https://doi.org/10.1007/s13225-015-0351-8

  15. Katoh, K. & Standley, D.M. (2013) MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Molecular Biology and Evolution 30: 772–780. https://doi.org/10.1093/molbev/mst010

  16. Landeros, F., Ferrusca-Rico, F.M., Guzman-Davalos, L., Esquivel-Naranjo, E.U., Matias-Ferrer, N., Burrola-Aguilar, C., Viurcos-Martinez, G. & Garibay-Orijel, R. (2021) Helvella jocatoi sp. nov. (Pezizales, Ascomycota), a new species from H. lacunosa complex with cultural importance in central Mexico Abies religiosa forests. Phytotaxa 498: 1–11. https://doi.org/10.11646/phytotaxa.498.1.1

  17. Landeros, F., Iturriaga, T. & Guzmán-Dávalos, L. (2012) Type studies in Helvella (Pezizales) 1. Mycotaxon 119: 35–63. http://dx.doi.org/10.5248/119.35

  18. Løken, S.B., Skrede, I. & Schumacher, T. (2020) The Helvella corium species aggregate in Nordic countries–phylogeny and species delimitation. Fungal systematics and evolution 5: 169–186. https://doi.org/10.3114/fuse.2020.05.11

  19. Nylander, J.A.A. (2004) MrModeltest v2, Program distributed by the author Evolutionary Biology Centre, Uppsala University.

  20. Rambaut, A. (2018) FigTree–Tree Figure Drawing Tool Version v. 1.4. 4. Institute of Evolutionary Biology, University of Edinburgh: Edinburgh.

  21. Rannala, B. & Yang, Z. (1996) Probability distribution of molecular evolutionary trees: a new method of phylogenetic inference. Journal of molecular evolution 43: 304–311.

  22. Skrede, I., Carlsen, T. & Schumacher, T. (2017) A synopsis of the saddle fungi (Helvella: Ascomycota) in Europe–species delimitation, taxonomy and typification. Persoonia-Molecular Phylogeny and Evolution of Fungi 39: 201–253. https://doi.org/10.3767/persoonia.2017.39.09

  23. Skrede, I., Gonzalvo, L.B., Mathiesen, C. & Schumacher, T. (2020) The genera Helvella and Dissingia (Ascomycota: Pezizomycetes) in Europe–Notes on species from Spain. Fungal Systematics and Evolution 6: 65–93. https://doi.org/10.3114/fuse.2020.06.05

  24. Tai, F.L. (1979) Sylloge Fungorum Sinicorum. Science Press, pp 155–157.

  25. Tedersoo, L., Hansen, K., Perry, B.A. & Kjøller, R. (2006) Molecular and morphological diversity of pezizalean ectomycorrhiza. New Phytologist 170: 581–596. http://dx.doi.org/10.1111/j.1469-8137.2006.01678.x

  26. Teng, S.C. (1963) Fungi of China. Science Press.

  27. Trifinopoulos, J., Nguyen, L.T., von Haeseler, A. & Minh, B.Q. (2016) W-IQ-TREE: a fast online phylogenetic tool for maximum likelihood analysis. Nucleic Acids Research 44: W232–W235. https://doi. org/10.1093/nar/gkw256

  28. Van Vooren, N. (2014) Notes sur le genre Helvella L. (Ascomycota, Pezizales). 2. Les sous-genres Cupuliformes et Macropodes. Bulletin mycologique et botanique Dauphiné-Savoie 212: 29–47.

  29. Vilgalys, R. & Hester, M. (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. Journal of bacteriology 172: 4238–4246.

  30. Wang, X.C., Liu, T.Z., Chen, S.L., Li, Y. & Zhuang, W.Y. (2019) A four-locus phylogeny of rib-stiped cupulate species of Helvella (Helvellaceae, Pezizales) with discovery of three new species. MycoKeys 60: 45–67. https://doi. org/10.3897/mycokeys.60.38186

  31. Wang, M., Zhao, Y.C., Zhao, Q. & Zhou, D.Q. (2016) Helvella sublactea sp. nov. (Helvellaceae) from southwestern China. Phytotaxa 253: 131–138. https://doi.org/10.11646/phytotaxa.253.2.2

  32. Weidemann, H.M. (1998) Påvisning av Helvella ektomykorrhiza hos Dryas og Salix ved hjelp av taxon-selektive nrDNA Helvella-primere (Detection of Helvella ectomycorrhiza on Dryas and Salix using taxon-selective nrDNA based Helvella primers) Cand. scient. thesis, Department of Biology, University of Oslo, Oslo, Norway. [In Norwegian.]

  33. Xu, R.J., Li, L. & Zhao, Q. (2022) Helvella cystidiata sp. nov. (Helvellaceae, Ascomycota) from Tibetan Plateau, China. Phytotaxa 560: 82–92. https://doi.org/10.11646/phytotaxa.560.1.6

  34. Zhao, Q., Sulayman, M., Zhu, X.T., Zhao, Y.C., Yang, Z.L. & Hyde, K.D. (2016a) Species clarification of the culinary Bachu mushroom in western China. Mycologia 108: 828–836. https://doi. org/10.3852/16-002

  35. Zhao, Q., Tolgor, B., Zhao, Y.C., Yang, Z.L. & Hyde, K.D. (2015) Species diversity within the Helvella crispa group (Ascomycota: Helvellaceae) in China. Phytotaxa 239: 130–142. http://dx.doi.org/10.11646/phytotaxa.239.2.2

  36. Zhao, Q., Zhang, X., Li, S., Chai, H., Bahkali, A.H. & Hyde, K.D. (2016b) New species and records of saddle fungi (Helvella, Helvellaceae) from Jiuzhaigou Natural Reserve, China. Mycoscience 57: 422–430. https://doi.org/10.1016/j.myc.2016.07.005

  37. Zhuang, W.Y., Zheng, H.D. & Zeng, Z.Q. (2018) Species catalogue of China. Volume 3: Fungi. Cup-Fungi. Science Press, pp. 1–142.