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Type: Article
Published: 2023-05-25
Page range: 187-205
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Two new records of Xylariales species from Northern Thailand

School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand. Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand. State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Medical University, Guiyang 550004, P.R. China.
School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand. Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand.
Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand
State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Medical University, Guiyang 550004, P.R. China
Doi Inthanon national park fungal diversity morphology multigene phylogeny Sordariomycetes taxonomy


Xylariales members have conspicuous to inconspicuous perithecia with unitunicate asci. The most known species are endophytes and saprobes, known to produce secondary metabolites with fundamental importance in the pharmaceutical and chemical industries. The current study collected samples from decaying barks of Quercus kingiana at Doi Inthanon National Park, Thailand. Based on morphological features coupled with combined gene analyses of ITS, LSU, rpb2 and tub2 sequence data, the isolates were identified as Xylaria karsticola and Jackrogersella minutella, belonging to Xylariaceae and Hypoxylaceae, respectively. This study provides the first geographical records of X. karsticola and J. minutella in Thailand and the first records of these species on Quercus kingiana.


  1. Anderson, R. (2009) Hypoxylon in Britain and Ireland 2. Hypoxylon rubiginosum and its allies. Field Mycology 9: 41–48.
    Doi Inthanon Military Base. (2001) Precipitation data recorded at the military base at the summit of Doi Inthanon (1982-1999).
    Becker, K. & Stadler, M. (2021) Recent progress in biodiversity research on the Xylariales and their secondary metabolism. The Journal of Antibiotics 74: 1–23.
    Bucher, V.V.C., Hyde, K.D., Pointing, S.B. & Reddy, C.A. (2004) Production of wood decay enzymes, mass loss and lignin solubilization in wood by marine ascomycetes and their anamorphs. Fungal Diversity 15: 1–14.
    Cedeño–Sanchez, M., Wendt, L., Stadler, M. & Mejía, L.C. (2020) Three new species of Hypoxylon and new records of Xylariales from Panama. Mycosphere 11: 1457–1476.
    Chuaseeharonnachai, C., Yamaguchi, K., Sri-Indrasutdhia, V., Somrithipol, S., Okanec, I., Nakagiri, A. & Boonyuen, N. (2013) Diversity of aero-aquatic hyphomycetes from six streams in Doi Inthanon and Khao Yai tropical forests, Thailand. Cryptogamie Mycologie 34: 183–197.
    Chethana, K.W.T., Manawasinghe, I.S., Hurdeal, V.G., Bhunjun, C.S., Appadoo, M.A., Gentekaki, E., Raspé, O., Promputtha, I. & Hyde, K.D. (2021) What are fungal species and how to delineate them? Fungal Diversity 109: 1–25.
    Chuzho, K. & Dkhar, M.S. (2019) Diversity of ascomycetous wood-rotting fungi along an altitudinal gradient in forests of Nagaland and first report of Jackrogersella minutella from India. Journal of the Indian Academy of Wood Science 16: 36–43.
    Daranagama, D.A., Hyde, K.D., Sir, E.B., Thambugala, K.M., Tian, Q., Samarakoon, M.C., McKenzie, E.H., Jayasiri, S.C., Tibpromma, S., Bhat, J.D. & Liu, X. (2018) Towards a natural classification and backbone tree for Graphostromataceae, Hypoxylaceae, Lopadostomataceae and Xylariaceae. Fungal Diversity 88: 1–165.
    Daranagama, D.A., Camporesi, E., Tian, Q., Liu, X., Chamyuang, S., Stadler, M. & Hyde, K.D. (2015) Anthostomella is polyphyletic comprising several genera in Xylariaceae. Fungal Diversity 73: 203–238.
    Darriba, D., Taboada, G.L., Doallo, R. & Posada, D. (2012) jModelTest 2: more models, new heuristics and high-performance computing. Nature methods 9: 772.
    Dayarathne, M.C., Maharachchikumbura, S.S.N., Jones, E.B.G., Goonasekara, I.D., Bulgakov, T.S., Al-Sadi, A.M., Hyde, K.D., Lumyong, S. & McKenzie, E.H.C. (2017) Neophyllachora gen nov. (Phyllachorales), three new species of Phyllachora from Poaceae and resurrection of Polystigmataceae (Xylariales). Mycosphere 10: 1598−1625.
    de Silva, N., Hyde, K.D., Lumyong, S., Phillips, A.J.L., Bhat, D.J., Maharachchikumbura, S.S.N., Thambugala, K.M., Tennakoon, D.S., Suwannarach, N. & Karunarathna, S.C. (2022) Morphology, phylogeny, host association and geography of fungi associated with plants of Annonaceae, Apocynaceae and Magnoliaceae. Mycosphere 13: 955–1076.
    Fournier, J., Flessa, F., Peršoh, D. & Stadler M. (2011) Three new Xylaria species from southwestern Europe. Mycological progress 10: 33–52.
    Hall, T.A. (1999) BioEdit: A user-friendly biological sequence alignment editor and analysis program for Windows 95/98/N.T. Nucleic Acids Symposium Series 41: 95–98.
    Hashemi, S.A., Khodaparast, S.A., Zare, R. & Elahinia, S.A. (2014) Contribution to the identification of Xylaria species in Iran. Rostaniha 15: 153–166.
    Hawksworth, D.L., Kirk, P.M., Sutton, B.C. & Pegler, D.N. (1995) Ainsworth and Bisby’s Dictionary of the Fungi, 8th edition. C.A.B. International: Oxon, U.K.
    Hernãndez-Restrepo, M., Decock, C.A., Costa, M.M. & Crous, P.W. (2022) Phylogeny and taxonomy of Circinotrichum, Gyrothrix, Vermiculariopsiella and other setose hyphomycetes. Persoonia 49: 99–135.
    Hsieh, H.M., Lin, C.R., Fang, M.J., Rogers, J.D., Fournier, J., Lechat, C. & Ju, Y.M. (2010) Phylogenetic status of Xylaria subgenus Pseudoxylaria among taxa of the subfamily Xylarioideae (Xylariaceae) and phylogeny of the taxa involved in the subfamily. Molecular Phylogenetics and Evolution 54: 957–969.
    Hsieh, H.M., Ju, Y.M. & Rogers, J.D. (2005) Molecular phylogeny of Hypoxylon and closely related genera. Mycologia 97: 844–865.
    Hurdeal, V.G., Jones, E.B.G., André-Luiz, C.M.De.A.S., Hyde, K.D. & Gentekaki, E. (2022) Expanding the diversity of mucoralean fungi from northern Thailand: novel Backusella species from soil. Phytotaxa 559: 275–284.
    Husbands, D.R., Urbina, H., Lewis, S.M. & Aime, M.C. (2018) Xylaria karyophthora: A new seed-inhabiting fungus of Greenheart from Guyana. Mycologia 110: 434–447.
    Hyde, K.D., Norphanphoun, C., Chen, J., Dissanayake, A.J., Doilom, M., Hongsanan, S., Jayawardena, R.S., Jeewon, R., Perera, R.H., Thongbai, B. & Wanasinghe, D.N. (2018) Thailand’s amazing diversity: up to 96% of fungi in northern Thailand may be novel. Fungal Diversity 93: 215–239.
    Hyde, K.D., Norphanphoun, C., Maharachchikumbura, S.S.N., Bhat, D.J., Jones, E.B.G., Bundhun, D., Chen, Y.J., Bao, D.F., Boonmee, S., Calabon, M.S., Chaiwan, N., Chethana, K.W.T., Dai, D.Q., Dayarathne, M.C., Devadatha, B., Dissanayake, A.J., Dissanayake, L.S., Doilom, M., Dong, W., Fan, X.L., Goonasekara, I.D., Hongsanan, S., Huang, S.K., Jayawardena, R.S., Jeewon, R., Karunarathna, A., Konta, S., Kumar, V., Lin, C.G., Liu, J.K., Liu, N.G., Luangsa-ard, J., Lumyong, S., Luo, Z.L., Marasinghe, D.S., McKenzie, E.H.C., Niego, A.G.T., Niranjan, M., Perera, R.H., Phukhamsakda, C., Rathnayaka, A.R., Samarakoon, M.C., Samarakoon, S.M.B.C., Sarma, V.V., Senanayake, I.C., Shang, Q.J., Stadler, M., Tibpromma, S., Wanasinghe, D.N., Wei, D.P., Wijayawardene, N.N., Xiao, Y.P., Yang, J., Zeng, X.Y., Zhang, S.N. & Xiang, M.M. (2020) Refined families of Sordariomycetes. Mycosphere 11: 305–1059.
    Index Fungorum (2023) Available from: (Accessed 10 January 2023).
    Jayasiri, S.C., Hyde, K.D., Ariyawansa, H.A., Bhat, J., Buyck, B., Cai, L., Dai, Y.C., Abd-Elsalam, K.A., Ertz, D., Hidayat, I., Jeewon, R., Jones, E.B.G., Bahkali, A.H., Karunarathna, S.C., Liu, J.K., Luangsa-ard, J.J., Lumbsch, H.T., Maharachchikumbura, S.S.N., McKenzie, E.H.C., Moncalvo, J.M., Ghobad-Nejhad, M., Nilsson, H., Pang, K.A., Pereira, O.L., Phillips, A.J.L., Raspé, O., Rollins, A.W., Romero, A.I., Etayo, J., Selçuk, F., Stephenson, S.L., Suetrong, S., Taylor, J.E., Tsui, C.K.M., Vizzini, A., Abdel-Wahab, M.A., Wen, T.C., Boonmee, S., Dai, D.Q., Daranagama, D.A., Dissanayake, A.J., Ekanayaka, A.H., Fryar, S.C., Hongsanan, S., Jayawardena, R.S., Li, W.J., Perera, R.H., Phookamsak, R., de Silva, N.I., Thambugala, K.M., Tian, Q., Wijayawardene, N.N., Zhao, R.L., Zhao, Q., Kang, J.C. & Promputtha, I. (2015) The Faces of Fungi database: fungal names linked with morphology, phylogeny and human impacts. Fungal Diversity 74: 3–18.
    Ju, Y.M. & Rogers, J.D. (1996) A revision of the genus Hypoxylon. American Phytopathological Society, Saint Paul, Minnesota, 382 pp.
    Kang, J.C., Kong, R.Y. & Hyde, K.D. (1998) Studies on the Amphisphaeriales 1. Amphisphaeriaceae (sensu stricto) and its phylogenetic relationships inferred from 5.88 rDNA and ITS2 sequences. Fungal Diversity 1: 147–157. []
    Kang, J.C., Kong, R.Y. & Hyde, K.D. (2002) Phylogeny of Amphisphaeriaceae (sensu stricto) and related taxa revisited based on nrDNA sequences. Mycotaxon 81: 321–330.
    Katoh, K., Rozewicki, J. & Yamada, K.D. (2019) M.A.F.F.T. online service: multiple sequence alignment, interactive sequence choice and visualization. Briefings in Bioinformatics 20: 1160–1166.
    Kim, C.S., Jo, J.W., Kwag, Y.N., Oh, S.O., Lee, S.G., Sung, G.H., Han, J.G., Oh, J., Shrestha, B., Kim, S.Y. & Shin, C.H. (2016) New records of Xylaria species in Korea: X. ripicola sp. nov. and X. tentaculata. Mycobiology 44: 21–28.
    Kirk, P.M., Cannon, P.F., Minter, D.W. & Stalpers, J.A. (2008) Ainsworth & Bisby’s dictionary of the fungi, 10th edn. CABI Publishing, Wallingford.
    Konta, S., Hyde, K.D., Phookamsak, R., Xu, J.C., Maharachchikumbura, S.S.N., Daranagama, D.A., McKenzie, E.H.C., Boonmee, S., Tibpromma, S., Eungwanichayapant, P.D. & Samarakoon, M.C. (2020) Polyphyletic genera in Xylariaceae (Xylariales): Neoxylaria gen. nov. and Stilbohypoxylon. Mycosphere 11: 2629–2651.
    Kuhnert, E., Sir, E.B., Lambert, C., Hyde, K.D., Hladki, A.I., Romero, A.I., Rohde, M. & Stadler, M. (2017) Phylogenetic and chemotaxonomic resolution of the genus Annulohypoxylon (Xylariaceae) including four new species. Fungal Diversity 85: 1–43.
    Læssøe, T., Srikitikulchai, P., Luangsa-ard, J.D. & Stadler, M. (2013) Theissenia reconsidered, including molecular phylogeny of the type species T. pyrenocrata and a new genus Durotheca (Xylariaceae, Ascomycota). IMA Fungus 4: 57–69.
    Lestari, A.S. & Chethana, K.W.T. (2022) Morpho-phylogenetic insights reveal Bisporella montana as Calycina montana comb. nov. (Pezizellaceae, Helotiales). Phytotaxa 558: 185–202.
    Li, J.F., Phookamsak, R., Jeewon, R., Tibpromma, S., Maharachchikumbura, S.S.N., Bhat, D.J., Chukeatirote, E., Lumyong, S., Hyde, K.D. & McKenzie, E.H.C. (2017) Establishment of Zygosporiaceae fam. nov. (Xylariales, Sordariomycetes) based on rDNA sequence data to accommodate Zygosporium. Mycosphere 8: 1855–1868.
    Liu, Y.J., Whelen, S. & Hall, B.D. (1999) Phylogenetic relationships among ascomycetes: evidence from an RNA polymerase II subunit. Molecular biology and evolution 16: 1799–1808.
    Lumbsch, H.T. & Huhndorf, S.M. (2009) Myconet volume 14 part one. Outline of Ascomycota. Fieldiana Life and Earth Sciences 1: 1–922.
    Ma, H., Song, Z., Pan, X., Qu, Z., Yang, Z., Li, Y. & Zhu, A. (2022) Four new pale-spored species of Xylaria (Xylariaceae, Xylariales) with a key to worldwide species on fallen fruits and seeds. Biology 11: 885.
    Ma, H.X. & Li, Y. (2018) Xylaria crinalis and X. betulicola from China—two new species with threadlike stromata. Sydowia 70: 37–49.
    Ma, H.X., Qiu, J.Z., Xu, B. & Li, Y. (2018) Two Hypoxylon species from Yunnan Province based on morphological and molecular characters. Phytotaxa 376: 27−36.
    Miller, M., Pfeiffer, W.T. & Schwartz, T. (2010) Creating the CIPRES science gateway for inference of large phylogenetic trees. In Proceedings of the Gateway Computing Environments Workshop: 14 Nov 2010. pp. 1–8.
    Munk, A. (1953) The system of the Pyrenomycetes. Dansk Botanisk Arkin 15: 1–163.
    Nannfeldt, J.A. (1932) Studien über die morphologie und systematik der nichtlichenisierten inoperculaten Discomyceten. Nova Acta Regiae Societatis Scientiarum Upsaliensis 8 (2): 1–368.
    Nordén, B. (2014) Annulohypoxylon minutellum and Obolarina dryophila (Xylariales), two stromatic pyrenomycetes on oak new to Norway. Agarica 35: 25–28.
    O’Donnell, K. & Cigelnik E. (1997) Two divergent intragenomic rDNA ITS2 types within a monophyletic lineage of the fungus Fusarium are nonorthologous. Molecular Phylogenetics and Evolution 7: 103–16.
    Pan, X.Y., Song, Z.K., Qu, Z., Liu, T.D. & Ma, H.X. (2022) Three new Xylaria species (Xylariaceae, Xylariales) on fallen leaves from Hainan Tropical Rainforest National Park. MycoKeys 86: 47–63. 10.3897/mycokeys.86.71623
    Perera, R.H., Hyde, K.D., Maharachchikumbura, S.S.N., Jones, E.B.G., McKenzie, E.H.C., Stadler, M., Lee, H.B., Samarakoon, M.C., Ekanayaka, A.H., Camporesi, E. & Liu, J.K. (2020) Fungi on wild seeds and fruits. Mycosphere 11: 2108–2480. 10.5943/mycosphere/11/1/14
    Persoh, D., Melcher, M., Graf, K., Fournier, J., Stadler, M. & Rambold, G. (2009) Molecular and morphological evidence for the delimitation of Xylaria hypoxylon. Mycologia 101: 256–268.
    Pointing, S.B., Pelling, A.L., Smith., GJD., Hyde, K.D. & Reddy, C.A. (2005) Screening of basidiomycetes and xylariaceous fungi for lignin peroxidase and laccase gene-specific sequences. Mycological Research 109: 115–124.
    Phutthacharoen, K., Chethana, K.W.T., Lestari, A.S., Stadler, M. & Hyde, K.D. (2022) Three New Species of Dicephalospora (Helotiaceae,Helotiales) from Thailand. Diversity 14: 645.
    Raei, S., Khodaparast, S.A. & Abbasi, M. (2012) Contribution to the knowledge of Hypoxylon and Annulohypoxylon in Guilan province (N Iran). Rostaniha 13: 197–206.
    Rambaut, A. (2014) FigTree v1.4. Institute of Evolutionary Biology, University of Edinburgh, Edinburgh. Available at: (accessed 24 May 2023)
    Roensch, P., Roensch, S., Reiher A. & Otto, P. (2010) Investigations on the fructicolous Xylaria delitschii and Xylaria Oxyacanthae. Boletus 32: 106–122.
    Rogers, J.D. & Candoussau, F. (1980) A new variety of Hypoxylon cohaerens from France. Mycologia 72: 826–829.
    Rogers, J.D. (2000) Thoughts and musings on tropical Xylariaceae. Mycological Research 104: 1412–1420.
    Samarakoon, M.C., Hyde, K.D., Promputtha, I., Hongsanan, S., Ariyawansa, H.A., Maharachchikumbura, S.S.N., Daranagama, D.A., Stadler, M. & Mapook, A. (2016) Evolution of Xylariomycetidae (Ascomycota: Sordariomycetes). Mycosphere 7: 1746–1761.
    Samarakoon, M.C., Hyde, K.D., Maharachchikumbura, S.S., Stadler, M., Gareth Jones, E.B., Promputtha, I., Suwannarach, N., Camporesi, E., Bulgakov, T.S. & Liu, J.K. (2022) Taxonomy, phylogeny, molecular dating and ancestral state reconstruction of Xylariomycetidae (Sordariomycetes). Fungal Diversity 112: 1–88.
    Sugita, R., Hirayama, K., Shirouzu, T. & Tanaka, K. (2022) Spirodecosporaceae fam. nov. (Xylariales, Sordariomycetes) and two new species of Spirodecospora. Fungal Systematics and Evolution 10: 217–229.
    Schmit, J.P. & Mueller, G.M. (2007) An estimate of the lower limit of global fungal diversity. Biodiversity and Conservation 16: 99–111.
    Senanayake, I.C., Maharachchikumbura, S.S.N., Hyde, K.D., Bhat, J.D., Jones, E.B.G., McKenzie, E.H.C., Dai, D.Q., Daranagama, D.A., Dayarathne, M.C., Goonasekara, I.D., Konta, S., Li, W.J., Shang, Q.J., Stadler, M., Wijayawardene, N.N., Xiao, Y.P., Norphanphoun, C., Li, Q., Liu, X.Z., Bahkali, A.H., Kang, J.C., Wang, Y., Wen, T.C., Wendt, L., Xu, J.C. & Camporesi, E. (2015) Towards unraveling relationships in Xylariomycetidae (Sordariomycetes). Fungal Diversity 73: 73–144.
    Smith, G.J.D., Liew, E.C. & Hyde, K.D. (2003) The Xylariales: A monophyletic order containing 7 families. Fungal Diversity 13: 185–218.
    Stadler, M., Kuhnert, E., Persŏh, D. & Fournier, J. (2013) The Xylariaceae as model example for a unified nomenclature following the ‘‘One fungus-one name’’ (1F1N) concept. Mycology 4: 5–21.
    Stamatakis, A. (2014) RAxML version 8: A tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 30: 1312–1313.
    Sydow, H. & Sydow, P. (1913) Enumeration of Philippine fungi, with notes and descriptions of new species. Part I. Micromycetes. Philippine Journal of Science 8: 265–285.
    Tang, A.M.C., Jeewon, R. & Hyde, K.D. (2005) Successional patterns of microfungi in fallen leaves of Castanopsis fissa (Fagaceae) in Hong Kong forest. Canadian Journal of Microbiology 51: 967–974.
    Thomas, D.C., Vandegrift, R., Ludden, A., Carroll, G.C. & Roy, B.A. (2016) Spatial ecology of the fungal genus Xylaria in a Tropical Cloud Forest. Biotropica 48: 381–393.
    Vilgalys, R. & Hester, M. (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. Journal of Bacteriology 172: 4238–4246.
    Voglmayr, H., Friebes, G., Gardiennet, A. & Jaklitsch, W.M. (2018) Barrmaelia and Entosordaria in Barrmaeliaceae (fam. nov., Xylariales) and critical notes on Anthostomella-like genera based on multigene phylogenies. Mycological Progress 17: 155–177.
    Watkinson, S., Boddy, L. & Money, N. (2015) The fungi, 3rd edn. Academic Press, London, 466 pp.
    Wendt, L., Sir, E.B., Kuhnert, E., Heitkämper, S., Lambert, C., Hladki, A.I., Romero, A.I., Luangsa-ard, J.J., Srikitikulchai, P., Peršoh, D. & Stadler, M. (2018) Resurrection and emendation of the Hypoxylaceae, recognised from a multigene phylogeny of the Xylariales. Mycological Progress 17: 115–154.
    Whalley, A.J.S. & Edwards, R.L. (1995) Secondary metabolites and systematic arrangement within the Xylariaceae. Canadian Journal of Botany 73: 802–810.
    White, T.J., Bruns, T., Lee, S. & Taylor, J. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis, M.A., Gelfand, D.H., Sninsky, J.J & White, T.J (Eds.) PCR Protocols: A Guide to Methods and Applications. Academic Press. San Diego California. pp. 315–322.
    Wibberg, D., Stadler, M., Lambert, C., Bunk, B., Spröer, C., Rückert, C., Kalinowski, J., Cox, R.J. & Kuhnert, E. (2021) High quality genome sequences of thirteen Hypoxylaceae (Ascomycota) strengthen the phylogenetic family backbone and enable the discovery of new taxa. Fungal Diversity 106: 7–28.
    Wijayawardene, N.N., Hyde, K.D., Dai, D.Q., Sánchez-García, M., Goto, B.T., Saxena, R.K., Erdoðdu, M., Selçuk, F., Rajeshkumar, K.C., Aptroot, A., Błaszkowski, J., Boonyuen, N., da Silva, G.A., de Souza, F.A., Dong, W., Ertz, D., Haelewaters, D., Jones, E.B.G., Karunarathna, S.C., Kirk, P.M., Kukwa, M., Kumla, J., Leontyev, D.V., Lumbsch, H.T., Maharachchikumbura, S.S.N., Marguno, F., Martínez-Rodríguez, P., Mešić, A., Monteiro, J.S., Oehl, F., Pawłowska, J., Pem, D., Pfliegler, W.P., Phillips, A.J.L., Pošta, A., He, M.Q., Li, J.X., Raza, M., Sruthi, O.P., Suetrong, S., Suwannarach, N., Tedersoo, L., Thiyagaraja, V., Tibpromma, S., Tkalčec, Z., Tokarev, Y.S., Wanasinghe, D.N., Wijesundara, D.S.A., Wimalaseana, S.D.M.K., Madrid, H., Zhang, G.Q., Gao, Y., Sánchez-Castro, I., Tang, L.Z., Stadler, M., Yurkov, A., Thines, M. (2022) Outline of Fungi and fungus-like taxa – 2021. Mycosphere 13: 53–453.
    Willis, K.J. (2018) State of the world’s fungi 2018–report. Royal Botanic Gardens, Kew, 92 pp.