Skip to main content Skip to main navigation menu Skip to site footer
Type: Article
Published: 2023-05-23
Page range: 111-123
Abstract views: 56
PDF downloaded: 5

Taxonomy and phylogeny of Hypoxylon zhaotongensis sp. nov. (Hypoxylaceae, Xylariales), a bambusicolous fungus from Yunnan, China

Center for Yunnan Plateau Biological Resources Protection and Utilization, College of Biological Resource and Food Engineering, Qujing Normal University, Qujing, P.R. China.
Center for Yunnan Plateau Biological Resources Protection and Utilization, College of Biological Resource and Food Engineering, Qujing Normal University, Qujing, P.R. China.
Center for Yunnan Plateau Biological Resources Protection and Utilization, College of Biological Resource and Food Engineering, Qujing Normal University, Qujing, P.R. China. Section of Genetics, Institute for Research and Development in Health and Social Care, Battaramulla, Sri Lanka.
Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.
Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia.
Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia.
Center for Yunnan Plateau Biological Resources Protection and Utilization, College of Biological Resource and Food Engineering, Qujing Normal University, Qujing, P.R. China.
Center for Yunnan Plateau Biological Resources Protection and Utilization, College of Biological Resource and Food Engineering, Qujing Normal University, Qujing, P.R. China.
Anthurium verapazense Catemaco Ecological reserve Santa Marta volcano Monocots

Abstract

During a survey of bambusicolous fungi in Yunnan Province, southwestern China, several saprobic fungi associated with dead bamboo culms were collected and cursorily identified as species belonging to Xylariales (Sordariomycetes), based on a morphological approach. In this study, a new species, Hypoxylon zhaotongensis, from bamboo was established. In addition, molecular analyses based on multiple gene sequences (ITS, LSU, RPB2, β-tubulin) were carried out to determine the phylogenetic placement of the new isolates. The morphological characteristics show that our new taxon differs from the known Hypoxylon species in having broader asci and ascospores with conspicuously straight germ-slit, which is less than the spore length. Detailed description, photo-plate and a phylogenetic tree showing the taxonomic placement of the new species are provided in this paper.

References

  1. Bulliard, P. (1791) Histoire des champignons de la France, ou Traité élémentaire renfermant dans un ordre méthodique les descriptions et les figures des champignons qui croissent naturellemnt en France, par M. Bulliard. Vol. 1.
    Cai, L. (2020) Preface to the Special issue on Fungal Resources and Diversity. Journal of fungi 39 (4): 3. https://doi.org/CNKI:SUN:JWXT.0.2020-04-002
    Cedeño-Sanchez, M., Charria-Girón, E., Lambert, C., Luangsa-ard, J.J., Decock, C., Franke, R., Brönstrup, M. & Stadler, M. (2023) Segregation of the genus Parahypoxylon (Hypoxylaceae, Xylariales) from Hypoxylon by a polyphasic taxonomic approach. MycoKeys 95: 131–162. https://doi.org/10.3897/mycokeys.95.98125
    Dai, D.Q., Phookamsak, R., Wijayawardene, N.N., Li, W.J., Bhat, D.J., Xu, J.C., Taylor, J.E., Hyde, K.D. & Chukeatirote, E. (2017) Bambusicolous fungi. Fungal Diversity 82 (1): 1–105. https://doi.org/10.1007/s13225-016-0367-8
    Dai, D.Q., Wijayawardene, N.N., Dayarathne, M.C., Kumla, J., Han, L.S., Zhang, G.Q., Zhang, X., Zhang, T.T. & Chen, H.H. (2022) Taxonomic and Phylogenetic Characterizations Reveal Four New Species, Two New Asexual Morph Reports, and Six New Country Records of Bambusicolous Roussoella from China. Journal of Fungi 8 (5): 532. https://doi.org/10.3390/jof8050532
    Daranagama, D.A., Hyde, K.D., Sir, E.B., Thambugala, K.M., Tian, Q., Samarakoon, M.C., McKenzie, E.H.C., Jayasiri, S.C., Tibpromma, S., Bhat, J.D., Liu, X.Z. & Stadler, M. (2018) Towards a natural classification and backbone tree for Graphostromataceae, Hypoxylaceae, Lopadostomataceae and Xylariaceae. Fungal Diversity 88: 1–165. https://doi.org/10.1007/s13225-017-0388-y
    Fournier, J., Lechat, C. & Courtecuisse, R. (2016) The genus Hypoxylon (Xylariaceae) in Guadeloupe and Martinique (French West Indies). Ascomycete org 7: 145–212.
    Glez-Peña, D., Gómez-Blanco, D., Reboiro-Jato, M., Fdez-Riverola, F. & Posada, D. (2010) ALTER: program-oriented conversion of DNA and protein alignments. Nucleic Acids Research 38: 14–18. https://doi.org/10.1093/nar/gkq321
    Hall, T. (2004) BioEdit. Ibis Therapeutics, Carlsbad, CA, 92008, USA. Available from: http://www.mbio.ncsu.edu/BioEdit/bioedit.html (accessed 18 March 2005)
    Helaly, S.E., Thongbai, B. & Stadler, M. (2018) Diversity of biologically active secondary metabolites from endophytic and saprotrophic fungi of the Ascomycete order Xylariales. Natural Products Report 35: 992–1014. https://doi.org/10.1039/C8NP00010G
    Hellwig, V., Ju, Y.M., Rogers, J.D., Fournier, J. & Stadler, M. (2005) Hypomiltin, a novel azaphilone from Hypoxylon hypomiltum, and chemotypes in Hypoxylon sect. Hypoxylon as inferred from analytical HPLC profiling. Mycological Progress 4: 39–54. https://doi.org/10.1007/s11557-006-0108-6
    Huelsenbeck, J.P. & Ronquist, F. (2001) MRBAYES: Bayesian inference of phylogenetic trees. Bioinformatics 17: 754–755. https://doi.org/10.1093/bioinformatics/17.8.754
    Jayasiri, S.C., Hyde, K.D., Ariyawansa, H.A., Bhat, J., Buyck, B., Cai, L., Dai, Y.C., Abd-Elsalam, K.A., Ertz, D., Hidayat, I., Jeewon, R., Jones, E.B.G., Bahkali, A.H., Karunarathna, S.C., Liu, J.K., Luangsa-ard, J.J., Lumbsch, H.T., Maharachchikumbura, S.S.N., McKenzie, E.H.C., Moncalvo, J.M., Ghobad-Nejhad, M., Nilsson, H., Pang, K.A., Pereira, O.L., Phillips, A.J.L., Raspé, O., Rollins, A.W., Romero, A.I., Etayo, J., Selçuk, F., Stephenson, S.L., Suetrong, S., Taylor, J.E., Tsui, C.K.M., Vizzini, A., Abdel-Wahab, M.A., Wen, T.C., Boonmee, S., Dai, D.Q., Daranagama, D.A., Dissanayake, A.J., Ekanayaka, A.H., Fryar, S.C., Hongsanan, S., Jayawardena, R.S., Li, W.J., Perera, R.H., Phookamsak, R., de Silva, N.I., Thambugala, K.M., Tian, Q., Wijayawarden, N.N., Zhao, R.L., Zhao, Q., Kang, J.C. & Promputtha, I. (2015) The Faces of fungi database: fungal names linked with morphology and phylogeny and human impacts. Fungal Diversity 74: 3–18. https://doi.org/10.1007/s13225-015-0351-8
    Jiang, H.B., Phookamsak, R., Hyde, K.D., Mortimer, P.E., Xu, J.C., Kakumyan, P. & Kumla, J. (2021) A taxonomic appraisal of bambusicolous fungi in Occultibambusaceae (Pleosporales, Dothideomycetes) with new collections from Yunnan Province, China. Life 11 (9): 932. https://doi.org/10.3390/life11090932
    Jiang, J.P., Du, C., Liu, B., Wang, K., Cai, L., Li, Q. & Huang, X.L. (2022) Bioinventory in China: progress and perspectives. Biodiversity Science 30: 22531. https://doi.org/10.17520/biods.2022531
    Ju, Y.M. & Rogers, J.D. (1996) A revision of the Genus Hypoxylon. American Phytopathological Society (APS Press).
    Katoh, K. & Standley, D.M. (2013) MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Molecular Biology and Evolution 30: 772–780. https://doi.org/10.1093/molbev/mst010
    Kuhnert, E., Fournier, J., Peršoh, D., Luangsa-ard, J.J.D. & Stadler, M. (2014a) New Hypoxylon species from Martinique and new evidence on the molecular phylogeny of Hypoxylon based on ITS rDNA and β- tubulin data. Fungal Diversity 64: 181–203. https://doi.org/10.1007/s13225-013-0264-3
    Kuhnert, E., Heitkämper, S., Fournier, J., Surup, F. & Stadler, M. (2014b) Hypoxyvermelhotins A-C, new pigments from Hypoxylon lechatii sp. nov. Fungal Biology 118: 242–252. https://doi.org/10.1016/j.funbio.2013.12.003
    Lambert, C., Wendt, L., Hladki, A.I., Stadler, M. & Sir, E.B. (2019) Hypomontagnella (Hypoxylaceae): A new genus segregated from Hypoxylon by a polyphasic taxonomic approach. Mycological Progress 18 (1–2): 187–201. https://doi.org/10.1007/s11557-018-1452-z
    Liese, W. & Köhl, M. (eds.) (2015) Bamboo: The Plant and its Uses. Springer International Publishing: Cham, Switzerland. 356 pp. https://doi.org/10.1007/978-3-319-14133-6
    Liu, Y., Whelen, S. & Hall, B. (1999) Phylogenetic relationships among Ascomycetes: evidence from an RNA polymerse II subunit. Molecular Biology and Evolution 16: 1799–1808. https://doi.org/10.1093/oxfordjournals.molbev.a026092
    Maharachchikumbura, S.S., Hyde, K.D., Jones, E.B.G., McKenzie, E.H.C., Huang, S.K., Abdel-Wahab, M.A., Daranagama, D.A., Dayarathne, M., D’souza, M.J., Goonasekara, I.D., Hongsanan, S., Jayawardena, R.S., Kirk, P.M., Konta, S., Liu, J.K., Liu, Z.Y., Norphanphoun, C., Pang, K.L., Perera, R.H., Senanayake, I.C., Shang, Q., Shenoy, B.D., Xiao, Y.P., Bahkali, A.H., Kang, J.C., Somrothipol, S., Suetrong, S., Wen, T.C. & Xu, J.C. (2015) Towards a natural classification and backbone tree for Sordariomycetes. Fungal Diversity 72: 199–301. https://doi.org/10.1007/s13225-015-0331-z
    Ma, H.X., Vasilyeva, L. & Li, Y. (2012) Hypoxylon from China-2: H. dengii sp. nov. and H. crocopeplum new to China. Mycotaxon 122: 1–5. https://doi.org/ 10.5248/122.1
    Ma, H.X., Qiu, J.Z., Xu, B. & Li, Y. (2018) Two Hypoxylon species from Yunnan Province based on morphological and molecular characters. Phytotaxa 376: 27–36. https://doi.org/10.11646/phytotaxa.376.1.3
    Ma, H., Song, Z., Pan, X., Li, Y., Yang, Z. & Qu, Z. (2022) Multi-Gene Phylogeny and Taxonomy of Hypoxylon (Hypoxylaceae, Ascomycota) from China. Diversity 14: 37. https://doi.org/ 10.3390/d14010037
    Manandhar, R., Kim, J.H. & Kim, J.T. (2017) Environmental, social and economic sustainability of bamboo and bamboo-based construction materials in buildings. Journal of Asian Architecture and Building Engineering 18: 49–59. https://doi.org/10.1080/13467581.2019.1595629
    Miller, M.A., Pfeiffer, W. & Schwartz, T. (2010) Creating the CIPRES Science Gateway for inference of large phylogenetic trees. In: 2010 Gateway Computing Environments Workshop (GCE). New Orleans, LA, USA, pp. 1–8. https://doi.org/10.1109/GCE.2010.5676129
    Nylander, J.A.A. (2004) MrModeltest 2.0. Program distributed by the author. Evolutionary Biology Centre, Uppsala University.
    O’donnell, K. & Cigelnik, E. (1997) Two divergent intragenomic rDNA ITS2 types within a monophyletic lineage of the fungus Fusarium are nonorthologous. Molecular Phylogenetics and Evolution 7: 103–116. https://doi.org/10.1006/mpev.1996.0376
    Phookamsak, R., Jiang, H., Suwannarach, N., Lumyong, S., Xu, J., Xu, S., Liao, C.F. & Chomnunti, P. (2022) Bambusicolous Fungi in Pleosporales: Introducing Four Novel Taxa and a New Habitat Record for Anastomitrabeculia didymospora. Journal of Fungi 8: 630. https://doi.org/ 10.3390/jof8060630
    Rambaut, A. (2006) FigTree. Tree drawing tool version 1.4. 0. University of Edinburgh: Institute of Evolutionary Biology. [http://tree.bio.ed.ac.uk/software/figtree/]
    Rannala, B. & Yang, Z. (1996) Probability distribution of molecular evolutionary trees: a new method of phylogenetic inference. Joural of molecular evolution 43: 304–311. https://doi.org/10.1007/BF02338839
    Rogers, J.D. (2018) The Xylariaceae Systematic, Biological and Evolutionary Aspects. Mycologia 71 (1): 1–42. https://doi.org/10.1080/00275514.1979.12020984
    Ronquist, F. & Huelsenbeck, J.P. (2003) MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19: 1572–1574. https://doi.org/10.1093/bioinformatics/btg180
    Senanayake, I.C., Rathnayaka, A.R., Marasinghe, D.S., Calabon, M.S., Gentekaki, E., Wanasinghe, D.N., Lee, H.B., Hurdeal, V.G., Pem, D., Dissanayake, L.S., Wijesinghe, S.N., Bundhun, D., Nguyen, T.T., Goonasekara, I.D., Abeywickrama, P.D., Bhunjun, C.S., Chomnunti, P., Boonmee, S., Jayawardena, R.S., Wijayawardene, N.N., Doilom, M., Jeewon, R., Bhat, J.D., Zhang, H.X. & Xie, N. (2020) Morphological approaches in studying fungi: collection, examination, isolation, sporulation and preservation. Mycosphere 11: 2678–2754. https://doi.org/10.5943/mycosphere/11/1/20
    Sir, E.B., Kuhnert, E., Lambert, C., Hladki, A.I., Romero, A.I. & Stadler, M. (2016) New species and reports of Hypoxylon from Argentina recognized by a polyphasic approach. Mycological Progress 15: 42. https://doi.org/10.1007/s11557-016-1182-z
    Stadler, M., Fournier, J., Granmo, A. & Beltrán-Tejera, E. (2008) The “red Hypoxylons” of the temperate and subtropical Northern hemisphere. North American Fungi 3: 73–125. https://doi.org/10.2509/naf2008.003.0075
    Stadler, M. (2011) Importance of secondary metabolites in the Xylariaceae as parameters for assessment of their taxonomy, phylogeny, and functional biodiversity. Current Research in Environmental & Applied Mycology 1: 75–133. https://doi.org/10.5943/cream/1/2/1
    Tamura, K., Stecher, G., Peterson, D., Filipski, A. & Kumar, S. (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution 30: 2725–2729. https://doi.org/10.1093/molbev/mst197
    Vilgalys, R. & Hester, M. (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. Journal of bacteriology 172: 4238–4246. https://doi.org/10.1128/jb.172.8.4238-4246.1990
    Wendt, L., Sir, E.B., Kuhnert, E., Heitkämper, S., Lambert, C., Hladki, A.I., Romero, A.I., Luangsa-ard, J.J., Srikitikulchai, P., Peršoh, D. & Stadler, M. (2018) Resurrection and emendation of the Hypoxylaceae, recognised from a multigene phylogeny of the Xylariales. Mycological Progress 17 (1–2): 115–154. https://doi.org/10.1007/s11557-017-1311-3
    White, T.J., Bruns, T., Lee, S. & Taylor, J. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis, M.A., Gelfand, D.H., Sninsky, J.J. & White, T.J. (Eds.) PCR Protocols: A guide to methods and applications. Academic Press, San Diego, pp. 315–322. https://doi.org/10.1016/B978-0-12-372180-8.50042-1
    Wijayawardene, N.N., Dissanayake, L.S., Dai, D.Q., Xiao, Y.P., Wen, T.C., Karunarathna, S.C., Wu, H.X., Zhang, H., Tibpromma, S., Kang, J.C., Wang, Y., Shen, X.C., Tang, L.Z., Deng, C.Y., Liu, Y.X. & Kang, Y.Q. (2021) Yunnan–Guizhou Plateau: a mycological hotspot. Phytotaxa 523 (1): 1–31 https://doi.org/10.11646/phytotaxa.523.1.1
    Wijayawardene, N.N., Dai, D.Q., Zhu, M.L., Wanasinghe, D.N., Kumla, J., Zhang, G.Q., Zhang, T.T., Han, L.S., Tibpromma, S. & Chen, H.H. (2022) Fungi associated with dead branches of Magnolia grandiflora: A case study from Qujing, China. Frontiers in Microbiology 13: 954680. https://doi.org/10.3389/fmicb.2022.954680
    Yang, E.F., Karunarathna, S.C., Dai, D.Q., Stephenson, S.L., Elgorban, A.M., Al-Rejaie, S., Xiong, Y.R., Promputtha, I., Samarakoon, M.C. & Tibpromma, S. (2022) Taxonomy and Phylogeny of Fungi Associated with Mangifera indica from Yunnan, China. Journal of Fungi 8: 1249. https://doi.org/10.3390/jof8121249
    Zhaxybayeva, O. & Gogarten, J.P. (2002) Bootstrap, Bayesian probability and maximum likelihood mapping: exploring new tools for comparative genome analyses. BMC Genomics 3: 4. https://doi.org/10.1186/1471-2164-3-4