Morphological and multigene phylogenetic analyses reveal two new nematode-trapping fungi (Arthrobotrys, Orbiliaceae) from Yunnan, China

YAO-QUAN YANG; FA ZHANG; ZHENG-QIANG LI; FA-PING ZHOU; XIAO-YAN YANG; WEN XIAO

doi:10.11646/phytotaxa.591.4.3

Issue: Vol. 591 No. 4: 11 April 2023

Type: Article

Published: 2023-04-11

DOI: 10.11646/phytotaxa.591.4.3

Page range: 263-272

Abstract views: 117

PDF downloaded: 5

**Morphological and multigene phylogenetic analyses reveal two new nematode-trapping fungi (Arthrobotrys, Orbiliaceae) from Yunnan, China**

YAO-QUAN YANG⁺⁻
FA ZHANG⁺⁻
ZHENG-QIANG LI⁺⁻
FA-PING ZHOU⁺⁻
XIAO-YAN YANG⁺⁻
WEN XIAO⁺⁻

Institute of Eastern-Himalaya Biodiversity Research, Dali University, Dali Yunnan 671003, P.R. China. The Provincial Innovation Team of Biodiversity Conservation and Utility of the Three Parallel Rivers Region, Dali Yunnan 671003, P.R. China.

Institute of Eastern-Himalaya Biodiversity Research, Dali University, Dali Yunnan 671003, P.R. China. The Provincial Innovation Team of Biodiversity Conservation and Utility of the Three Parallel Rivers Region, Dali Yunnan 671003, P.R. China. Collaborative Innovation Center for Biodiversity and Conservation in the Three Parallel Rivers Region of China, Dali Yunnan 671003, P.R. China.

Institute of Eastern-Himalaya Biodiversity Research, Dali University, Dali Yunnan 671003, P.R. China. The Provincial Innovation Team of Biodiversity Conservation and Utility of the Three Parallel Rivers Region, Dali Yunnan 671003, P.R. China.

Institute of Eastern-Himalaya Biodiversity Research, Dali University, Dali Yunnan 671003, P.R. China. The Provincial Innovation Team of Biodiversity Conservation and Utility of the Three Parallel Rivers Region, Dali Yunnan 671003, P.R. China. Collaborative Innovation Center for Biodiversity and Conservation in the Three Parallel Rivers Region of China, Dali Yunnan 671003, P.R. China.

Institute of Eastern-Himalaya Biodiversity Research, Dali University, Dali Yunnan 671003, P.R. China. The Provincial Innovation Team of Biodiversity Conservation and Utility of the Three Parallel Rivers Region, Dali Yunnan 671003, P.R. China. Collaborative Innovation Center for Biodiversity and Conservation in the Three Parallel Rivers Region of China, Dali Yunnan 671003, P.R. China. Yunling black-and-white snub-nosed monkey observation and research station of Yunnan province, Dali Yunnan 671003, P.R. China.

2 new species Carnivorous fungi Morphology Phylogeny Fungi

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Abstract

During the investigation of nematode-trapping fungi in Yunnan Province, four strains were isolated from the soil collected in Nujiang City, Yunnan Province, China and were identified as two new Arthrobotrys (Orbiliaceae) species based on morphology and multigene (ITS, EF1-α, and RPB2) phylogenetic analysis. Arthrobotrys gongshanensis is characterized by conidiophores producing 1–5 short denticles near the apex, bearing a single conidium on each denticle and obovoid to fusiform, 1–4-septate conidia. Arthrobotrys luzhangensis is characteristics by its obovoid to fusiform, 1–4-septate macroconidia, and clavate or obovoid, aseptate microconidia. These two new species both capture nematodes using adhesive networks. The multigene phylogenetic analyses further confirmed the taxonomic positions of these two new species within Arthrobotrys. This paper described them with illustrations and compared them with similar taxa.

References

Ahrén, D., Ursing, B.M. & Tunlid, A. (1998) Phylogeny of nematode-trapping fungi based on 18S rDNA sequences. FEMS Microbiology Letters 158 (2): 179–184. https://doi.org/10.1016/S0378-1097(97)00519-3
Barron, G.L. (1977) The nematode-destroying fungi. Canadian Biological Publications Ltd., Guelph, Ontario, 45 pp.
Barron, G.L. (1979) Nematophagous fungi: a new Arthrobotrys with nonseptate conidia. Canadian Journal of Botany 57 (12): 1371–1373. https://doi.org/10.1139/b79-170
Corda, A.K.J. (1839) Pracht-flora europaeischer Schimmelbildungen. G. Fleischer, 43 pp.
Drechsler, C. (1937) Some hyphomycetes that prey on free-living terricolous nematodes. Mycologia 29 (4): 447–552. https://doi.org/10.1080/00275514.1937.12017222
Duddington, C.L. (1951) Two new predacious hyphomycetes. Transactions of the British Mycological Society 34 (4): 598–603. https://doi.org/10.1016/S0007-1536(51)80044-5
Felsenstein, J. (1985) Confidence limits on phylogenies: An approach using the bootstrap. Evolution 39: 783–791. https://doi.org/10.1111/j.1558-5646.1985.tb00420.x
Gao, R.H., Lei, L.P. & Liu, X.Z. (1996) A simple method for inducing and observing predacious devices of nematode-trapping fungi. Acta Mycologica Sinica 4: 304–305+326. https://doi.org/10.13346/j.mycosystema.1996.04.013
Hall, B.G. (2007) Phylogenetic Trees Made Easy: A How-to Manual. Massachusetts: Sinauer Associates Sunderland: Sunderland, MA, USA.
Hall, T.A. (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 41: 95–98 https://doi.org/ 10.12691/ajmr-3-2-1
Huelsenbeck, J.P. & Ronquist, F. (2001) MRBAYES: Bayesian inference of phylogenetic trees. Bioinformatics 17 (8): 754–755.
Katoh, K. & Standley, D.M. (2013) MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Molecular biology and evolution 30 (4): 772–780. https://doi.org/10.1093/molbev/mst010
Kirk, P.M., Cannon, P.F., Minter, D.W. & Stalpers, J.A. (2008) Dictionary of the Fungi. CAB International, Oxon UK.
Li, J., Liu, Y., Zhu, H. & Zhang, K.Q. (2016) Phylogenic analysis of adhesion related genes Mad1 revealed a positive selection for the evolution of trapping devices of nematode-trapping fungi. Scientific reports 6 (1): 1–10. https://doi.org/10.1038/srep22609
Li, J., Qian, W., Qiao, M., Bai, Y. & Yu, Z. (2013) A new Drechslerella species from Hainan, China. Mycotaxon 125: 183–188. https://doi.org/10.5248/125.183
Li, T.F., Zhang, K.Q. & Liu, X.Z. (2000) Taxonomy of Nematophagous Fungi. Chinese Scientific and Technological Publications, Beijing China, pp.35.
Liou, G.Y. & Tzean, S.S. (1997) Phylogeny of the genus Arthrobotrys and allied nematode-trapping fungi based on rDNA sequences. Mycologia 89 (6): 876–884. https://doi.org/10.1080/00275514.1997.12026858
Liu, S.R., Su, H.Y., Su, X.J., Zhang, F., Liao, G.H. & Yang, X.Y. (2014) Arthrobotrys xiangyunensis, a novel nematode-trapping taxon from a hot-spring in Yunnan Province, China. Phytotaxa 174 (2): 89–96. http://dx.doi.org/10.11646/phytotaxa.174.2.3
Liu, Y.J., Whelen, S. & Hall, B.D. (1999) Phylogenetic relationships among ascomycetes: evidence from an RNA polymerse II subunit. Molecular biology and evolution 16 (12): 1799–1808. https://doi.org/10.1093/oxfordjournals.molbev.a026092
Nguyen, L.T., Schmidt, H.A., Von Haeseler, A. & Minh, B.Q. (2015) IQ-TREE: a fast and effective stochastic algorithm for estimating maximum-likelihood phylogenies. Molecular biology and evolution 32 (1): 268–274. https://doi.org/10.1093/molbev/msu300
O’Donnell, K., Kistler, H.C., Cigelnik, E. & Ploetz, R.C. (1998) Multiple evolutionary origins of the fungus causing Panama disease of banana: concordant evidence from nuclear and mitochondrial gene genealogies. Proceedings of the National Academy of Sciences 95 (5): 2044–2049. https://doi.org/10.1073/pnas.95.5.2044
Posada, D. (2008) jModelTest: phylogenetic model averaging. Molecular biology and evolution 25 (7): 1253–1256. https://doi.org/10.1093/molbev/msn083
Quijada, L., Baral, H.O., Beltrán-Tejera, E. & Pfister, D.H. (2020) Orbilia jesu-laurae (Ascomycota, Orbiliomycetes), a new species of neo-tropical nematode-trapping fungus from Puerto Rico, supported by morphology and molecular phylogenetics. Willdenowia 50: 241–251. https://doi.org/10.3372/wi.50.50210
Rambaut, A. (2021) FigTree v1. 3.1. 2010. Available Online: http://tree.bio.ed.ac.uk/software/figtree/ (accessed 5 March 2021).
Rezakhani, F., Khodaparast, S.A., Masigol, H., Roja-Jimenez, K., Grossart, H.P. & Bakhshi, M. (2019) A preliminary report of aquatic hyphomycetes isolated from Anzali lagoon (Gilan province, North of Iran). Rostaniha 20: 123–143. https://doi.org/10.22092/BOTANY.2019.126701.1161
Schenck, S., Kendrick, W.B. & Pramer, D. (1977) A new nematode-trapping hyphomycete and a reevaluation of Dactylaria and Arthrobotrys. Canadian Journal of Botany 55 (8): 977–985. https://doi.org/10.1139/b77-115
Su, H.Y., Li, M. & Liu, X.X. (2006) Improvement of the slide making method of nematode trapping fungi. Journal of dali university 2006 (06): 25–26+30.
Su, H.Y., Liu, S.R., Li, Y.X. & Cao, Y.H. (2011) Arthrobotrys latispora, a new nematode-trapping fungus from southwest China. Mycotaxon 117 (3): 29–36. [https://www.researchgate.net/publication/278130299]
Swe, A., Jeewon, R. & Hyde, K.D. (2008) Nematode-Trapping fungi from mangrove habitats. Cryptogamie Mycologie 29 (4): 333–354. [https://www.researchgate.net/publication/288671904]
Swe, A., Li, J., Zhang, K.Q., Pointing, S.B., Jeewon, R. & Hyde, K.D. (2011) Nematode-trapping fungi. Current Research in Environmental & Applied Mycology 1 (1): 1–26.
Tamura, K., Stecher, G., Peterson, D., Filipski, A. & Kumar, S. (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Molecular biology and evolution 30 (12): 2725–2729. https://doi.org/10.1093/molbev/mst197
Vu, D., Groenewald, M., De Vries, M., Gehrmann, T., Stielow, B., Eberhardt, U., Al-Hatmi, A., Gronewald, J.Z., Cardinali, G. & Houbraken, J. (2019) Large-scale generation and analysis of filamentous fungal DNA barcodes boosts coverage for kingdom fungi and reveals thresholds for fungal species and higher taxon delimitation. Studies in Mycology 92: 135–154. https://doi.org/10.1016/j.simyco.2018.05.001
White, T.J., Bruns, T., Lee, S. & Taylor, J. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR protocols: a guide to methods and applications 18 (1): 315–322. https://doi.org/10.1016/b978-0-12-372180-8.50042-1
Wu, H.Y., Kim, D.G., Ryu, Y.H. & Zhou, X.B. (2012) Arthrobotrys koreensis, a new nematode-trapping species from Korea. Sydowia 64: 129–136.
Yang, E., Xu, L.L., Yang, Y., Zhang, X.Y., Xiang, M.C., Wang, C.S., An, Z.Q. & Liu, X.Z. (2012) Origin and evolution of carnivorism in the Ascomycota (fungi). PNAS 109: 10960–10965. https://doi.org/10.1073/pnas.1120915109
Yang, Y., Yang, E., An, Z., Liu, X.Z. (2007) Evolution of nematode-trapping cells of predatory fungi of the Orbiliaceae based on evidence from rRNA-encoding DNA and multiprotein sequences. PNAS 104: 8379–8384. https://doi.org/10.1073/pnas.0702770104
Zhang, F., Boonmee, S., Bhat, J.D., Xiao, W. & Yang, X.Y. (2022) New Arthrobotrys Nematode-Trapping Species (Orbiliaceae) from Terrestrial Soils and Freshwater Sediments in China. Journal of Fungi 8 (7): 671. https://doi.org/10.3390/jof8070671
Zhang, F., Zhou, X.J., Monkai, J., Li, F.T., Liu, S.R., Yang, X.Y., Xiao, W. & Hyde, K.D. (2020) Two new species of nematode-trapping fungi (Dactylellina, Orbiliaceae) from burned forest in Yunnan, China. Phytotaxa 452 (1): 65–74. https://doi.org/10.11646/phytotaxa.452.1.6
Zhang, K.Q. & Hyde, K.D. (2014) Nematode-trapping fungi. Springer Science & Business, Berlin.
Zhang, K.Q., Liu, X.Z., Cao, L. & Gao, R.H. (1996) A new species of Arthrobotrys from China. Mycological Research 5 (100): 527–530. https://doi.org/10.1016/S0953-7562(96)80003-7
Zhang, K.Q. & Mo, M.H. (2006) Flora Fungorum Sinicorum: Arthrobotrys et Gengra Cetera Cognata. Science Press, Beijing China.
Zhang, Y., Qiao, M., Baral, H., Xu, J., Zhang, K.Q. & Yu, Z.F. (2020) Morphological and molecular characterization of Orbilia pseudopolybrocha and O. tonghaiensis, two new species of Orbiliaceae from China. International journal of systematic and evolutionary microbiology 70 (4): 2664–2676. https://doi.org/10.1099/ijsem.0.004088