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Published: 2022-12-15
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Molecular and morphological notes on Opuntia ser. Streptacanthae (Cactaceae)

Posgrado en Biotecnología Agrícola, Instituto de Horticultura, Departamento de Fitotecnia, Universidad Autónoma Chapingo, Chapingo, México, 56230, Texcoco, Estado de México, México
Posgrado en Biotecnología Agrícola, Instituto de Horticultura, Departamento de Fitotecnia, Universidad Autónoma Chapingo, Chapingo, México, 56230, Texcoco, Estado de México, México
Posgrado en Biotecnología Agrícola, Instituto de Horticultura, Departamento de Fitotecnia, Universidad Autónoma Chapingo, Chapingo, México, 56230, Texcoco, Estado de México, México
Posgrado en Biotecnología Agrícola, Instituto de Horticultura, Departamento de Fitotecnia, Universidad Autónoma Chapingo, Chapingo, México, 56230, Texcoco, Estado de México, México
Cactaceae cactus pear Opuntia phylogeny relationships taxonomy Eudicots

Abstract

A detailed morphological and molecular study of the four Opuntia species belonging to the ser. Streptacanthae is carried out. No published phylogenetic study of Opuntia includes these four species. Our data support the recognition of the four taxa as separated species but related by the characteristic of having a glabrous epidermis, yellow flowers, red and sweet fruits, in the present study it is recovered forming a monophyletic group within Opuntia s.str.

References

<p>Anderson, E.F. (2001) <em>The Cactus family</em>. Timber Press, 776 pp.</p>
<p>Arias, S., Gama S. &amp; Guzmán, U. (1997) <em>Cactaceae. Flora del Valle de Tehuacán-Cuicatlán</em>, Fasc. 14. Instituto de Biología, Universidad Nacional Autónoma de México. México City, 146 pp.</p>
<p>Arias, S., Gama, S., Guzmán, U. &amp; Vázquez, B. (2012) Cactaceae. <em>Flora del Valle de Tehuacán-Cuicatlán</em>, Fasc. 95. Instituto de Biología, Universidad Nacional Autónoma de México. México City, 235 pp.</p>
<p>Bárcenas, R.T., Yesson, C.J. &amp; Hawkins, J.A. (2011) Molecular systematics of the Cactaceae. <em>Cladistics</em> 27: 470–489. https://dx.doi.org/10.1111/j.1096-0031.2011.00350.x</p>
<p>Benson, L. (1969) <em>The Native Cacti of California</em>. Stanford University Press, Staford, 243 pp.</p>
<p>Benson, L. (1982) <em>The Cacti of the United States and Canada</em>. Stanford University Press, Staford, 1044 pp.</p>
<p>Bouckaert, R., Heled, J., Kühnert, D., Vaughan, T., Wu, C.-H., Xie, D., Suchard, M.A., Rambaut, A. &amp; Drummond, A.J. (2014) BEAST 2: A software platform for Bayesian Evolutionary analysis. <em>PLOS Computational Biology </em>10: e1003537. https://doi.org/10.1371/journal.pcbi.1003537</p>
<p>Bravo-Hollis, H. (1937) <em>Las Cactáceas de México.</em> Universidad Nacional Autónoma de México. México City, 755 pp.</p>
<p>Bravo-Hollis, H. (1978) <em>Las Cactáceas de México</em>, vol. 1. Universidad Nacional Autónoma de México, México City, 351 pp.</p>
<p>Britton, N.L. &amp; Rose, J.N. (1919) <em>The Cactaceae: Description and illustrations of plants of the Cactus Family</em>, vol. 1. Carnegie Institution, Washington, 236 pp.</p>
<p>Dong, W., Xu, C., Li, C., Sun, J., Zuo, Y., Shi, S., Cheng, T., Guo, J. &amp; Zhou, S. (2015) <em>ycf1</em>, the most promising plastid DNA barcode of land plants. <em>Scientific Reports</em> 5: 1–5. 8348.&nbsp; https://doi.org/10.1038/srep08348</p>
<p>Frandsen, P.B., Calcott, B., Mayer, C. &amp; Lanfear, R. (2015) Automatic selection of partitioning schemes for phylogenetic analyses using iterative <em>k</em>-means clustering of site rates. <em>BMC Evolutionary Biology</em> 15: 1–17. https://doi.org/10.1186/s12862-015-0283-7</p>
<p>García, E. (1973) <em>Modificaciones al sistema de clasificación climática de Köppen</em>. 4<sup>th</sup> Edition. Printed by the Author, Mexico City, 217 pp.</p>
<p>García-Morales, L.J., García-Jiménez, J., Contreras-Medina, R., Alcántara-Ayala, O. &amp; Luna-Vega, I. (2022) Diversity, distribution and conservation of the Cactaceae (Caryophyllales) from Tamaulipas, Mexico. <em>Plant Biosystems </em>156: 1–17.&nbsp; https://doi.org/10.1080/11263504.2022.2056648</p>
<p>Griffith, M.P. (2004) The origins of an important cactus crop, <em>Opuntia ficus-indica</em> (Cactaceae): new molecular evidence. <em>American Journal of Botany</em> 91: 1915–1921. https://doi.org/10.3732/ajb.91.11.1915</p>
<p>Griffith, M.P. &amp; Porter, M. (2009) Phylogeny of Opuntioideae (Cactaceae). <em>International Journal of Plant Sciences</em> 170: 107–116. http://dx.doi.org/10.1086/593048</p>
<p>Griffiths, D. (1914) New species of <em>Opuntia</em>. <em>Biological Society</em> of <em>Washington</em> 27: 23–28.</p>
<p>Guiggi, A. (2015) Genera nova et combinations novae in Cactaceis Austroamericanis ad subfamiliam Opuntioideae K. Schumann spectantibus IV. <em>Supplementum to Cactology V:</em> 1–4.</p>
<p>Guzmán, U.L., Arias, S. &amp; Dávila, P. (2003) <em>Catálogo de cactáceas mexicanas.</em> Universidad Nacional Autónoma de México. México City, 315 pp.</p>
<p>Hall, T.A. (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. <em>Nucleic Acids Symposium Series</em> 41: 95–98.</p>
<p>Hernández, H.M., Gómez, H.C., Bárcenas, R.T., Puente, P. &amp; Reyes, A.J. (2014) A checklist of the subfamily Opuntioideae (Cactaceae) from North and Central America. <em>In</em>: Hunt, D.R. (Ed.) <em>Further Studies in Opuntioideae (Cactaceae). Succulent Plant Research</em> 8: 185–200.</p>
<p>Hernández-Hernández, T., Hernández, H., De-Nova, J.A., Puente, R., Eguiarte, L.E. &amp; Magallón, S. (2011) Phylogenetic relationships and evolution of growth form in Cactaceae (Caryophyllales, Eudicotyledoneae). <em>American Journal of Botany</em> 98: 44–61. https://dx.doi.org/10.3732/ajb.1000129</p>
<p>Huelsenbeck, J.P. &amp; Ronquist, F. (2001) MrBayes: Bayesian inference of phylogeny. <em>Bioinformatics </em>17 (8): 754–755. https://doi.org/10.1093/bioinformatics/17.8.754</p>
<p>Huelsenbeck, J.P., Larget, B. &amp; Alfaro, M.E. (2004) Bayesian phylogenetic model selection using reversible jump Markov Chain Montecarlo. <em>Molecular Biology and Evolution </em>21: 1123–1133. https://doi.org/10.1093/molbev/msh123</p>
<p>Hunt, D. (1999) CITES <em>Cactaceae, checklist</em>, 2<sup>nd</sup> Edition. Royal Botanical Gardens, Kew, 315 pp.</p>
<p>Hunt, D. (2006) <em>The New cactus lexicon</em>. International Cactaceae Systematics Group, Milbourne Port, 373 pp.</p>
<p>Hunt, D. (2014)<em> Further Studies in Opuntioideae (Cactaceae).</em> <em>Succulent Plant Research</em> 8: 185–200.</p>
<p>Katoh, K. &amp; Standley, D.M. (2013) MAFFT multiple sequence alignment software version 7: improvements in performance and usability. <em>Molecular Biology and Evolution </em>30 (4): 772–780. https://doi.org/10.1093/molbev/mst010</p>
<p>Katoh, K., Misawa, K., Kuma, K. &amp; Miyata, T. (2002) MAFFT: a novel method for rapid multiple sequence alignment based on fast Fourier transform. <em>Nucleic Acids Research </em>30 (14): 3059–3066. https://doi.org/10.1093/nar/gkf436</p>
<p>Katoh, K., Rozewicki, J. &amp; Yamada, K.D. (2017) MAFFT online service: multiple sequence alignment, interactive sequence choice and visualization. <em>Briefings in Bioinformatics</em> 20 (4): 1160–1166. https://doi.org/10.1093/bib/bbx108</p>
<p>Köhler, M., Font, F. &amp; Souza-Chies, T.T. (2018) First record of <em>Opuntia rioplatense</em> (Cactaceae) for the Brazilian flora. <em>Phytotaxa</em> 379 (4): 293–296.&nbsp; https://doi.org/10.11646/phytotaxa.379.4.3</p>
<p>Köhler, M., Font, F., Puente-Martínez, R. &amp; Majure, L.C. (2021a) “That´s <em>Opuntia</em>, that was!”, again: a new combination for an old and enigmatic <em>Opuntia</em> s.l. (Cactaceae). <em>Phytotaxa</em> 505 (3): 262–274. https://doi.org/10.11646/phytotaxa.505.3.2</p>
<p>Köhler, M., Oakley, L.J., Font, F., Las Peñas, M.L. &amp; Majure, L.C. (2021b) On the continuum of evolution: a putative new hybrid speciation event in <em>Opuntia</em> (Cactaceae) between a native and an introduced species in southern South America. <em>Systematics and Biodiversity</em> 19 (8): 1026–1039. https://doi.org/10.1080/14772000.2021.1967510</p>
<p>Korotkova, N., Aquino, D., Arias, S., Eggli, U., Franck, A., Gómez-Hinostrosa, C., Guerrero, P.C., Hernández, H.M., Kohlbecker, A., Köhler, M., Luther, K., Majure, L.C., Müller, A., Metzing, D., Nyffeler, R., Sánches, D., Schlumpberger, B. &amp; Berendsohn, W.G. (2021) Cactaceae at Caryophyllales.org–a dynamic online species-level taxonomic backbone for the family. <em>Willdenowia</em> 51: 251–270. https://doi.org/10.3372/wi.51.51208</p>
<p>Lanfear, R., Calcott, B., Kainer, D., Mayer, C. &amp; Stamatakis, A. (2014) Selecting optimal partitioning schemes for phylogenomic datasets. <em>BMC Evolutionary Biology</em> 14<em>: </em>1–14.&nbsp; https://doi.org/10.1186/1471-2148-14-82</p>
<p>Lanfear, R., Frandsen, P.B., Wright, A.M., Senfeld, T. &amp; Calcott, B. (2016) PartitionFinder 2: New methods for selecting partitioned models of evolution for molecular and morphological phylogenetic analyses. <em>Molecular Biology and Evolution</em> 34 (3): 772–773.&nbsp; https://doi.org/10.1093/molbev/msw260</p>
<p>Lemaire, C. (1839) <em>Cactearum genera nova speciesque novae et omnium in horto Monvilliano cultarum ex affinitatibus naturalibus ordinatio nova indexque methodicus</em>. Lutetiis, Paris, 115 pp.</p>
<p>Lodé, J. (2015) <em>Taxonomy of the Cactaceae (Supplement).</em> Alphabetical INDEX of taxa in current usage and their synonyms. Cactus Adventures International, Barcelona, 46 pp.</p>
<p>Maddison, W.P. &amp; Maddison, D.R. (2021) <em>Mesquite: a modular system for evolutionary analysis</em>. Version 3.70. Available from: https://mesquiteproject.org/ (accesed 16 April 2021)</p>
<p>Majure, L. &amp; Puente, R. (2014) Phylogenetic relationships and morphological evolution in <em>Opuntia</em> s.str. and closely related members of tribe Opuntieae. <em>Succulent Plant Research</em> 8: 9–30.</p>
<p>Majure, L., Puente, R., Griffith, M.P., Judd, W.S., Soltis, P.S. &amp; Soltis, D.E. (2012) Phylogeny of <em>Opuntia</em> s.s. (Cactaceae): clade delineation, geographic origins, and reticulate evolution. <em>American Journal of Botany</em> 99: 847–864. https://doi.org/10.3732/ajb.1100375</p>
<p>Majure, L., Puente, R., Griffith, M.P., Soltis, D.E. &amp; Judd, W.S. (2013) <em>Opuntia lilae</em>, another <em>Tacinga</em> hidden in <em>Opuntia </em>s.l. <em>Systematic Botany</em> 38: 444–450.</p>
<p>Majure, L., Köhler, M. &amp; Font, F. (2020) North American Opuntias (Cactaceae) in Argentina? Remarks on the phylogenetic position of <em>Opuntia penicilligera</em> and a closer look at <em>O. ventanensis</em>. <em>Phytotaxa</em> 428 (3): 279–289. https://doi.org/10.11646/phyto­taxa.428.3.9</p>
<p>Martínez-González, C.R. &amp; Morales-Sandoval, J. (2021) Taxonomic status of two controversial species of <em>Opuntia</em> (Cactaceae) from Mexico based on morphological and molecular data.<em> Bradleya</em> 39: 23–35.</p>
<p>Martínez-González, C.R., Gallegos-Vázquez, C., Luna-Vega, I. &amp; García-Sandoval, R. (2015a) <em>Opuntia leiascheinvariana</em>, una nueva especie de Cactaceae del Estado de Hidalgo, México. <em>Botanical Sciences</em> 93 (3): 517–529.</p>
<p>Martínez-González, C.R., Luna-Vega, I., Gallegos-Vázquez, C. &amp; García-Sandoval, R. (2015b) <em>Opuntia delafuentiana</em> (Cactaceae: Opuntioideae), a new xoconostle from central Mexico. <em>Phytotaxa</em> 231 (3): 230–244.</p>
<p>Martínez-González, C.R., Ramírez-Mendoza, R., Jiménez-Ramírez, J., Gallegos-Vázquez, C. &amp; Luna-Vega, I. (2017) Improved method for genomic DNA extraction for <em>Opuntia</em> Mill. (Cactaceae). <em>Plant Methods</em> 13: 1–10.&nbsp; https://doi.org/10.1186/s13007-017-0234-y</p>
<p>Martínez-González, C.R., Gallegos-Vázquez, C., Scheinvar, L., Muñiz-Díaz de León, M.E. &amp; Jiménez-Ramírez, J. (2019) Re-evaluation of <em>Opuntia matudae</em> (Cactaceae). <em>Phytotaxa</em> 423 (3): 158–170. https://doi.org/10.11646/phytotaxa.423.3.4</p>
<p>Martínez-González, C.R., Muñiz-Díaz de León, M.E., González-Martínez, C.A., Jiménez-Ramírez, J., Morales-Sandoval, J. &amp; Gallegos-Vázquez, C. (2020) Phylogenetic placement and new data on macro- and micromorphology of <em>Opuntia joconostle </em>(Cactaceae). <em>Bradleya </em>38: 158–169. https://doi.org/10.25223/brad.n38.2020.a17</p>
<p>Miller, P. (1754) <em>The gardeners dictionary: containing the methods of cultivating and improving all sorts of trees, plants, and flowers, for the kitchen, fruit, and pleasure gardens, and also those which are used in medicine: with directions for the culture of vineyards, and making wine in England</em>. Printed by the author, London, no pagination. https://doi.org/10.5962/bhl.title.79061</p>
<p>Mercado-Muñoz, F., León de la Luz, J.L., Rebman, J.P., Medel Narváez, A. &amp; Campos Ramos, R. (2021) Two new species of nopal (<em>Opuntia</em>, Cactaceae) from the Baja California Peninsula (Mexico). <em>Phytotaxa</em> 508 (3): 266–278. https://doi.org/10.11646/phytotaxa.508.3.2</p>
<p>Müller, K., Quandt, D., Müller, J. &amp; Neinhuis, C. (2005) <em>PhyDE®-Phylogenetic data editor. Program distributed by the authors, versión 10.0.</em> Available from: https://www.phyde.de (accessed 16 April 2021)</p>
<p>Nyffeler, R. (2002) Phylogenetic relationships in the cactus family (Cactaceae) based on evidence from <em>trnK/ matK </em>and <em>trnL-trnF </em>sequences. <em>American Journal of Botany </em>89 (2): 312–326.</p>
<p>Nyffeler, R. &amp; Eggli, U. (2010) A farewell to dated ideas and concepts: molecular phyogenetics and a revised suprageneric classification of the family Cactaceae. <em>Schumannia</em> 6: 109–149.</p>
<p>Ochoterena, I. (1922) <em>Las Cactáceas de México</em>. Editorial Cultura. México City, 189 pp.</p>
<p>Parfitt, B.D. &amp; Pinkava, D.J. (1988) Nomenclatural systematic reassessment of <em>Opuntia engelmanii </em>and <em>Opuntia lindheimeri </em>(Cactaceae). <em>Madroño</em> 35: 342–349.</p>
<p>Pfeiffer, L.G.K. (1837) <em>Enumeratio diagnostica Cactearum Hucusque Cognitarum</em>. Sumtibus Ludovici Oehmigke, Berlin, 192 pp. https://doi.org/10.5962/bhl.title.147375</p>
<p>Pinkava, D.J. (1996) Nomenclatural changes in <em>Opuntia</em> (Cactaceae). <em>Haseltonia</em> 4: 103–104.</p>
<p>Pinkava, D.J. (2002) On the evolution of the continental North American Opuntioideae. Studies in the Opuntioideae (Cactaceae). <em>Succulent Plant Research</em> 6: 59–98.</p>
<p>Porras-Flórez, D., Albesiano, S. &amp; Arrieta-Violet, L. (2020) Typification of the name <em>Opuntia soederstromiana</em> (Cactaceae), a new record for the Flora of Colombia. <em>Phytotaxa</em> 452 (2): 116–123. https://doi.org/10.11646/phytotaxa.452.2.1</p>
<p>Rambaut, A., Suchard, M.A., Xie, D. &amp; Drummond, A.J. (2014) <em>Tracer v1.6</em>. Available from: http://beast.bio.ed.ac.uk/Tracer (accessed 16 April 2021)</p>
<p>Reyes-Agüero, J.A., Aguirre, J.R., Carlín, F. &amp; González A. (2009) <em>Catálogo de las principales variantes silvestres y cultivadas de Opuntia en la Altiplanicie Meridional de México</em>. UASLP., SAGARPA and CONACYT, San Luis Potosí, México, 350 pp.</p>
<p>Rzedowski, J. (1978) <em>Vegetación de México</em>. Limusa, Mexico City, 432 pp.</p>
<p>Salm-Dyck, J. (1834) <em>Hortus Dyckensis oder Verzeichniss der in dem botanischen Garten zu Dyck wachsenden Pflanzen</em>. Düsseldorf, Arnz.</p>
<p>Scheinvar, L. (1974) <em>Opuntia heliabravoana</em>, una especie nueva de Cactaceae. <em>Anales del Instituto de Biología, Universidad Nacional Autónoma de México, Serie Botánica</em> 45: 75–86.</p>
<p>Scheinvar, L. (1976) <em>Opuntia rzedowskii</em> ‘Nopal de pedregal’, una especie nueva de Cactaceae. <em>Anales del Instituto de Biología, Universidad Nacional Autónoma de México, serie Botánica </em>53: 123–136.</p>
<p>Scheinvar, L. (1981) Species, variedades y combinaciones nuevas de cactáceas del Valle de México. <em>Phytologia</em> 49: 324–328.</p>
<p>Scheinvar, L. (1982) Redescubrimiento de la <em>Opuntia spinulifera</em> Salm-Dyck.<em> Anales del Instituto de Biología, Universidad Nacional Autónoma de México, Serie Botánica </em>47: 1–20.</p>
<p>Scheinvar, L. (1985) Cactaceae.<em> In:</em> Rzedowski, J. &amp; Calderón de Rzedowski, G. (Eds.) <em>La flora del Valle de México,</em> vol. 2, Instituto de Ecología, A. C. y Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional. City, pp. 93–115.</p>
<p>Scheinvar, L. (1987) Redescubrimiento de <em>Opuntia oligacantha</em> Forster en el Valle de México y en la Altiplanicie Mexicana. <em>Anales del Instituto de Biología, Universidad Nacional Autónoma de México, Serie Botánica</em> 57: 109–122.</p>
<p>Scheinvar, L. (1999) <em>Opuntia zamudioi</em>, una nueva especie de Querétaro. <em>Cactáceas y Suculentas Mexicanas</em> 44: 88–93.</p>
<p>Scheinvar, L. (2002) <em>Opuntia stricta</em> (Haw.) Haw. subsp. <em>esparzae</em>, una nueva subespecie de las dunas del río Concá, Arroyo Seco, Querétaro, México. <em>Cactáceas y Suculentas Mexicanas</em> 47: 94–102.</p>
<p>Scheinvar, L. (2004) <em>Flora cactológica del estado de Querétaro. Diversidad y riqueza.</em> Fondo de Cultura Económica, México, 390 pp.</p>
<p>Scheinvar, L. &amp; Olalde, G. (2008) Tipificación de algunas cactáceas del estado de Guerrero. <em>Cactáceas y Suculentas Mexicanas </em>53: 36–50.</p>
<p>Scheinvar, L. &amp; Rodríguez-Fuentes, A. (2003) Nueva subespecie de <em>Opuntia streptacantha</em> (Cactaceae) de la altiplanicie mexicana. <em>Anales del Instituto de Biología, Universidad Nacional Autónoma de México, Serie Botánica</em> 74: 303–311.</p>
<p>Scheinvar, L., Gallegos-Vázquez, C., Olalde-Parra, G. &amp; Rodríguez-Fuentes, A. (2010) <em>Opuntia</em> ser, <em>Streptacanthae</em> (Cactaceae)-neo- typifications and taxonomic notes for four species. <em>Schumannia</em> 6: 277–296.</p>
<p>Schmitz-Linneweber, C., Maier, R., Jean-Pierre, A., Cottet, A., Herrmann, R. &amp; Mache, R. (2001) The plastid chromosome of spinach (<em>Spinacia oleracea</em>): complete nucleotide sequence and gene organization. <em>Plant Molecular Biology</em> 45: 307–315.</p>
<p>Shaw, J., Lickey, E.B., Schilling, E.E. &amp; Small, R.L. (2007) Comparison of whole chloroplast genome sequences to choose noncoding regions for phylogenetic studies in angiosperms: The tortoise and the hare III. <em>American Journal of Botany</em> 94: 275–288. https://doi.org/10.3732/ajb.94.3.275</p>
<p>Stamatakis, A. (2014) RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. <em>Bioinformatics</em> 30: 1312–1313. https://doi.org/10.1093/bioinformatics/btu033</p>
<p>Stuppy, W. (2002) Seed characters and generic classification of the Opuntioideae (Cataceae). <em>Succulent Plant Research</em> 6: 25–58.</p>
<p>Taberlet, P.G., Ludovic, P., Guy, P. &amp; Bouvet, J. (1991) Universal primers for amplification of three non-coding regions of chloroplast DNA. <em>Plant Molecular Biology</em> 17: 1105–1109. https://doi.org/10.1007/BF00037152</p>
<p>Thiers, B. (2022) <em>Index herbariorum: a global directory of public herbaria and associated staff.</em> New York Botanical Gardenʾs Virtual Herbarium. Available from: http://sweetgum.nybg.org/ih (accessed 27 July 2022)</p>
<p>Weber, F.A.C. (1898) <em>Opuntia</em> Tournef. <em>In</em>: Bois, D. (Ed.) <em>Dictionnaire d´Horticulture</em>. P. Klincksieck, Paris, pp. 892–899.</p>
<p>White, TJ., Bruns, T.D., Lee, S. &amp; Taylor, J.W. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenies. <em>In</em>: Innis, M.A., Gelfand, D.H., Sninsky, J.J. &amp; White, T. (Eds<em>.</em>)<em> PCR Protocols: a guide to methods and applications</em>. Academic Press, San Diego, pp. 135–322.</p>
<p>Wilcox, C.D., Dove, S., McDavis, W. &amp; Greer, D. (2002) <em>UTHSCSA. Image Tool</em>. version 3.0. University of Texas Health Center, San Antonio, 56 pp.</p>
<p>Zhang, Z., Schwartz, S., Wagner, L. &amp; Miller, W. (2000) A greedy algorithm for aligning DNA sequences. <em>Journal of Computational Biology </em>7: 203–214.</p>