Ophiocordyceps ovatospora sp. nov. (Ophiocordycipitaceae, Hypocreales), pathogenic on termites from China

DEXIANG TANG; JUYE ZHU; LIJUN LUO; DONGHAI HOU; ZHIQIN WANG; SHIDAO YANG; HONG YU

doi:10.11646/phytotaxa.574.1.8

Issue: Vol. 574 No. 1: 25 November 2022

Type: Article

Published: 2022-11-25

DOI: 10.11646/phytotaxa.574.1.8

Page range: 105-117

Abstract views: 80

PDF downloaded: 1

Ophiocordyceps ovatospora sp. nov. (Ophiocordycipitaceae, Hypocreales), pathogenic on termites from China

DEXIANG TANG⁺⁻
JUYE ZHU⁺⁻
LIJUN LUO⁺⁻
DONGHAI HOU⁺⁻
ZHIQIN WANG⁺⁻
SHIDAO YANG⁺⁻
HONG YU⁺⁻

Yunnan Herbal Laboratory, College of Ecology and Environmental Sciences, Yunnan University, Kunming 650504, China.

Yunnan Herbal Laboratory, College of Ecology and Environmental Sciences, Yunnan University, Kunming 650504, China. School of Life Science, Yunnan University, Kunming, Yunnan 650504, China.

Yunnan Herbal Laboratory, College of Ecology and Environmental Sciences, Yunnan University, Kunming 650504, China.

1 new species morphology phylogeny taxonomy Fungi

Requires Subscription or Fee PDF (4MB)

Abstract

A new species, Ophiocordyceps ovatospora, occurring on termites was collected from Yunnan Province, China, and is described based on morphological and molecular phylogenetic data. In a phylogenetic analysis of a combined dataset of multigene loci comprising the internal transcribed spacer regions (ITS), the small and large subunit of the ribosomal DNA (nrSSU and nrLSU), partial sequences of elongation factor 1-alpha (tef-1a) and the largest and second largest subunit of the RNA polymerase (rpb1, rpb2), O. ovatospora was clustered together with O. khokpasiensis to form a clade separate from other species of Ophiocordyceps. Morphologically, O. ovatospora is characterized by producing pseudo-immersed perithecia, filiform asci and ascospores, monophialidic phialides, and oval conidia. Ophiocordyceps ovatospora has smaller perithecia than those of O. khokpasiensis but its asci are larger.

References

Abe, T., Bignell, D.E. & Higashi, M. (2000) Termites: Evolution, Sociality, Symbioses, Ecology. Kluwer Academic Publishers, London, United Kingdom, 247 pp. https://doi.org/10.1007/978-94-017-3223-9
Araújo, J.P.M., Evans, H.C., Geiser, D.M., Mackay, W.P. & Hughes, D.P. (2015) Unravelling the diversity behind the Ophiocordyceps unilateralis (Ophiocordycipitaceae) complex: three new species of zombie-ant fungi from the Brazilian Amazon. Phytotaxa 220: 224–238. https://doi.org/10.11646/phytotaxa.220.3.2
Araújo, J.P.M., Evans, H.C., Kepler, R.M. & Hughes, D.P. (2018) Zombie-ant fungi across continents: 15 new species and new combinations within Ophiocordyceps. I. Myrmecophilous hirsutelloid species. Studies in Mycology 90: 119–160. https://doi.org/10.1016/j.simyco.2017.12.002
Bischoff, J.F., Rehner, S.A. & Humber, R.A. (2006) Metarhizium frigidum sp. nov.: a cryptic species of M. anisopliae and a member of the M. flavoviride complex. Mycologia 98: 737–745. https://doi.org/10.3852/mycologia.98.5.737
Blackwell, M. & Gilbertson, R.L. (1981) Cordycepioideus octosporus, a termite suspected pathogen from Jalisco, Mexico. Mycologia 73: 358–362. https://doi.org/10.2307/3759659
Blackwell, M. & Gilbertson, R.L. (1984) New information on Cordycepioideus bisporus and Cordycepioideus octosporus. Mycologia 76: 763–765. https://doi.org/10.2307/3793239
Buckley, R.E. (2005) A Beauveria phylogeny inferred from nuclear ITS and EF1-α sequences: evidence for cryptic diversification and links to Cordyceps teleomorphs. Mycologia 97: 84–98. https://doi.org/10.1080/15572536.2006.11832842
Castlebury, L.A., Rossman, A.Y., Sung, G.H., Hyten, A.S. & Spatafora, J.W. (2004) Multigene phylogeny reveals new lineage for Stachybotrys chartarum, the indoor air fungus. Mycological Research 108: 864–872. https://doi.org/10.1017/S0953756204000607
Eggleton, P. (2000) Termites: evolutions, sociality symbioses, ecology. Kluwer Academic Publishers, Dordrecht. pp. 25–51. 
Evans, H.C., Elliot, S.L., Hughes, D.P. & Corrie, M. (2011) Hidden diversity behind the zombie-ant fungus Ophiocordyceps unilateralis: four new species described from carpenter ants in Minas Gerais, Brazil. PLoS One 6: e17024. https://doi.org/10.1371/journal.pone.0017024
Khonsanit, A., Luangsa-ard, J.J., Thanakitpipattana, D., Kobmoo, N. & Piasai1, O. (2018) Cryptic species within Ophiocordyceps myrmecophila complex on formicine ants from Thailand. Mycological Progress 18: 147–161. https://doi.org/10.1007/s11557-018-1412-7
Kirk, P.M., Cannon, P.F., Minter, D.W. & Stalpers, J.A. (2008) Ainsworth & Bisby’s dictionary of the fungi, 10th edn. CABI International, Wallingford. 771 pp.
Kobayasi, Y. (1941) The genus Cordyceps and its allies. Science Reports of the Tokyo Bunrika Daigaku 84: 53–260.
Kobmoo, N., Mongkolsamrit, S., Tasathai, K., Thanakitpipattana, D. & Luangsa-ard, J.J. (2012) Molecular phylogenies reveal host-specific divergence of Ophiocordyceps unilateralis sensu lato following its host ants. Molecular Ecology 21: 3022–3031. https://doi.org/10.1111/j.1365-294X.2012.05574.x
Kobmoo, N., Mongkolsamrit, S., Wutikhun, T., Tasanathai, K., Khonsanit, A., Thanakitpipattana, D. & Luangsa-Ard, T.T. (2015) New species of Ophiocordyceps unilateralis, an ubiquitous pathogen of ants from Thailand. Fungal Biology 119: 44–52. https://doi.org/10.1016/j.funbio.2014.10.008
Larkin, M.A., Blackshields, G., Brown, N.P., Chenna, R., McGettigan, P.A., McWilliam, H., Valentin, F., Wallace, I.M., Wilm, A., Lopez, R., Thompson, J.D., Gibson, T.J. & Higgins, D.G. (2007) Clustal W and Clustal X version 2.0. Bioinformatics 23: 2947–2948. https://doi.org/10.1093/bioinformatics/btm404
Liu, Y.J., Whelen, S. & Hall, B.D. (1999) Phylogenetic relationships among ascomycetes: evidence from an RNA polymerse II subunit. Molecular Biology and Evolution 16: 1799–1808. https://doi.org/10.1093/oxfordjournals.molbev.a026092
Liu, Z., Liang, Z.Q., Whalley, A.J.S., Yao, Y.J. & Liu, A.Y. (2001) Cordyceps brittlebankisoides, a new pathogen of grubs and its anamorph, Metarhizium anisopliae var. majus. Journal of Invertebrate Pathology 78: 178–182. https://doi.org/10.1006/jipa.2001.5039
Luangsa-ard, J.J., Ridkaew, R., Tasanathai, K., Thanakitpipattana, D. & Hywel-Jones, N. (2010) Ophiocordyceps halabalaensis: a new species of Ophiocordyceps pathogenic to Camponotus gigas in Hala Bala Wildlife Sanctuary, Southern Thailand. Fungal Biology 115: 608–614. https://doi.org/10.1016/j.funbio.2011.03.002
Luangsa-ard, J.J., Tasanathai, K., Thanakitpipattana, D., Khonsanit, A. & Stadler, M. (2018) Novel and interesting Ophiocordyceps spp. (Ophiocordycipitaceae, Hypocreales) with superficial perithecia from Thailand. Studies in Mycology 89: 125–142. https://doi.org/10.1016/j.simyco.2018.02.001
Ochiel, G.S., Evans, H.C. & Eilenberg, J. (1997) Cordycepioideus, a pathogen of termites in Kenya. Mycologist 11: 7–9. https://doi.org/10.1016/S0269-915X(97)80059-6
Pearce, M. & Pearce, M. (1997) Termites: biology and pest management. Ecological Entomology 23: 354–354. https://doi.org/10.1046/j.1365-2311.1998.00139.x
Petch, T. (1931) Notes on entomogenous fungi. Transactions of the British Mycological Society 16: 55–75. https://doi.org/10.1016/S0007-1536(31)80006-3
Rath, A.C. (2000) The use of entomopathogenic fungi for control of termites. Biocontrol Science and Technology 10: 563–581. https://doi.org/10.1080/095831500750016370.
Rehner, S.A. & Samuels, G.J. (1994) Taxonomy and phylogeny of Gliocladium analysed from nuclear large subunit ribosomal DNA sequences. Mycological Research 98: 625–634. https://doi.org/10.1016/S0953-7562(09)80409-7
Ronquist, F. & Huelsenbeck, J.P. (2003) MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19: 1572–1574. https://doi.org/10.1093/bioinformatics/btg180
Spatafora, J.W., Quandt, C.A., Kepler, R.M., Sung, G.H., Shrestha, B., Hywel-Jones, N.L. & Luangsa-ard, J.J. (2015) New 1F1N species combinations in Ophiocordycipitaceae (Hypocreales). IMA Fungus 6: 357–362. https://doi.org/10.5598/imafungus.2015.06.02.07
Stamatakis, A. (2006) RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics 22: 2688–2690. https://doi.org/10.1093/bioinformatics/btl446
Stifler, C.B. (1941) A new genus of Hypocreales. Mycologia 33: 82–86.
Sung, G.H., Hywel-Jones, N.L., Sung, J.M., Luangsa-ard, J.J., Shrestha, B. & Spatafora, J.W. (2007) Phylogenetic classification of Cordyceps and the clavicipitaceous fungi. Studies in Mycology 57: 5–59. https://doi.org/10.3114/sim.2007.57.01
Swofford, D.L. (2002) PAUP*. Phylogenetic analysis using parsimony (*and other methods), Version 4.0b10. Sinauer Associates, Sunderland, Massachusetts. https://doi.org/10.1111/j.0014-3820.2002.tb00191.x
Tamura, K., Stecher, G., Peterson, D., Filipski, A. & Kumar, S. (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution 30: 2725–2729.
Tasanathai, K., Noisripoom, W., Chaitika, T., Khonsanit, A., Hasin, S. & Luangsa-ard, J. (2019) Phylogenetic and morphological classification of Ophiocordyceps species on termites from Thailand. MycoKeys 56: 101–129. https://doi.org/10.3897/mycokeys.56.37636
Vilgalys, R. & Hester, M. (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. Journal of Bacteriology 172: 4238–4246. https://doi.org/10.1128/jb.172.8.4238-4246.1990 
Wang, Y.B., Yu, H., Dai, Y.D., Chen, Z.H., Zeng, W.B., Yuan, F. & Liang, Z.Q. (2015) Polycephalomyces yunnanensis (Hypocreales), a new species of Polycephalomyces parasitizing Ophiocordyceps nutans and stink bugs (hemipteran adults). Phytotaxa 208: 34–44. https://doi.org/10.11646/phytotaxa.208.1.3
Wang, Y.B., Wang, Y., Fan, Q., Duan, D.E., Zhang, G.D., Dai, R.Q., Dai, Y.D., Zeng, W.B., Chen, Z.H., Li, D.D., Tang, D.X., Xu, Z.H., Sun, T., Nguyen, T.T., Tran, N.L., Dao, V.M., Zhang, C.M., Huang, L.D., Liu, Y.J., Zhang, X.M., Yang, D.R., Sanjuan, T., Liu, X.Z., Yang, Z.L. & Yu, H. (2020) Multigene phylogeny of the family Cordycipitaceae (Hypocreales): new taxa and the new systematic position of the Chinese cordycipitoid fungus Paecilomyces hepiali. Fungal Diversity 103: 1–46. https://doi.org/10.1007/S13225-020-00457-3
White, T.J., Bruns, T., Lee, S. & Taylor, J. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR Protocols 315–322. https://doi.org/10.1016/b978-0-12-372180-8.50042-1
Xu, Z.H., Tran, N.L., Wang, Y., Zhang, G.D., Dao, V.M., Nguyen, T.T., Wang, Y.B. & Yu, H. (2022) Phylogeny and morphology of Ophiocordyceps puluongensis sp. nov. (Ophiocordycipitaceae, Hypocreales), a new fungal pathogen on termites from Vietnam. Journal of Invertebrate Pathology 192: 107771. https://doi.org/10.1016/j.jip.2022.107771