Cordyceps yaoluopingensis sp. nov., a new entomopathogenic species from China

YU YANG; YUAN-PING XIAO; YAN-NI CHENG; FEI-YAN XU; HAO-MING HAN; XIA LUO

doi:10.11646/phytotaxa.571.3.2

Issue: Vol. 571 No. 3: 4 November 2022

Type: Article

Published: 2022-11-04

DOI: 10.11646/phytotaxa.571.3.2

Page range: 278-290

Abstract views: 110

PDF downloaded: 2

Cordyceps yaoluopingensis sp. nov., a new entomopathogenic species from China

YU YANG⁺⁻
YUAN-PING XIAO⁺⁻
YAN-NI CHENG⁺⁻
FEI-YAN XU⁺⁻
HAO-MING HAN⁺⁻
XIA LUO⁺⁻

School of Biological Science and Food Engineering, Chuzhou University, No.1 West Huifeng Road, Chuzhou 239000, China. Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand. School of Food and Pharmaceutical Engineering, Guizhou Institute of Technology, Guiyang 550003, P.R. China.

Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand. School of Food and Pharmaceutical Engineering, Guizhou Institute of Technology, Guiyang 550003, P.R. China.

School of Biological Science and Food Engineering, Chuzhou University, No.1 West Huifeng Road, Chuzhou 239000, China.

insect fungi morphology multilocus phylogeny new taxon taxonomy

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Abstract

Entomopathogenic fungi have evolved in concert with insects for a long time and possess unique insecticidal properties and significant biological control, and medicinal potential. However, they are frequently overlooked and many previously unknown species have yet to be discovered and described. Cordyceps yaoluopingensis, a new species from Yaoluoping Nature Reserve in Anhui Province, China is defined as a new species based on morphology and molecular phylogenetic analyses. The new species has shorter synnemata, longer phialides and larger ovoid or fusiform conidia. Phylogenetic analysis using the six loci ITS, LSU, SSU, tef1-α, rpb1 and rpb2 established the uniqueness of the new species.

References

Cao, S.Q., Li, C.R., Huang, B., Fan, M.Z. & Li, Z.Z. (2010) Cordyceps cardinalis, a new species in China and its Isaria anamorph. Journal of Anhui Agricultural University 37 (4): 712–715. https://doi.org/10.1080/00949651003724790
Castillo, L.P., Osorio, A. Vargas, N., Sanjuan, T., Grajales, A. & Restrepo, S. (2018) Genetic diversity of the entomopathogenic fungus Cordyceps tenuipes in forests and butterfly gardens in Quindio, Colombia. Fungal Biology 122 (9): 891–899. https://doi.org/10.1016/j.funbio.2018.05.003
Castlebury, L.A., Rossman, A.Y., Sung, G.H., Hyten, A.S. & Spatafora, J.W. (2004) Multigene phylogeny reveals new lineage for Stachybotrys chartarum, the indoor air fungus Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA. Mycological Research 108 (8): 864–872. https://doi.org/10.1017/S0953756204000607
Cheek, M., Lughadha, E.N., Kirk, P.M., Lindon, H. & Niskanen, T. (2020) New scientific discoveries: Plants and fungi. Plants People Planet 2 (5): 371–388. https://doi.org/10.1002/ppp3.10148
Chen, W., Han, Y., Liang, J. & Liang, Z. (2021) Taxonomic and phylogenetic characterizations reveal four new species of Simplicillium (Cordycipitaceae, Hypocreales) from Guizhou, China. Scientific Reports 11 (1): 15300. https://doi.org/10.1038/s41598-021-94893-z
Chen, W., Liu, C., Han, Y.F., Liang, J.D., Tian, W.Y. & Liang, Z.Q. (2019a) Three novel insect-associated species of Simplicillium (Cordycipitaceae, Hypocreales) from Southwest China. MycoKeys 58: 83–102. https://doi.org/10.3897/mycokeys.58.37176
Chen, Z., Wang, Y., Dai, Y., Chen, K., Xu, L. & He, Q. (2019b) Species diversity and seasonal fluctuation of entomogenous fungi of Ascomycota in Taibaoshan Forest Park in western Yunnan. Biodiversity Science 27 (9): 993–1001. https://doi.org/ 10.17520/biods.2019135
Dhanasekaran, V., Mostert, L., Jeewon, R., Hyde, K. & Crous, P. (2004) Pleurostomophora, an anamorph of Pleurostoma (Calosphaeriales), a new anamorph genus morphologically similar to Phialophora. Studies in Mycology 50 (2): 50.
Fan, M.Z., Wang, B., Ding, D.G., Zhang, L., Wang, L.M. & Yu, Z.N. (1998) Investigation on the Resources of Entomogenous Fungi in Yaoluoping Nature Preservation. Journal of Anhui Agricultural University 25.
Gao, L. (2004) Investigation,Analysis and Protection of Some Special Plants in Yaolouping Natural Safeguard Areas. Journal of Anhui Agricultural Sciences 06: 1184–1186. https://doi.org/10.13989/j.cnki.0517-6611.2004.06.040
Gao, L., Xu, R.-S., Cao, J.X. & Zhou, Z.Y. (2002) A preliminary study on the flore of Yaoluoping National Resources Reserve. Joural of Biology. 06: 32–34. https://doi.org/10.13989/j.cnki.0517-6611.2004.06.040
Hall, T., Biosciences, I. & Carlsbad, C. (2011) BioEdit: An important software for molecular biology. GERF Bulletin of Biosciences 2 (1): 60–61.
He, Y., Wu, W. & Li, N. (2016) A Checklist of Macrofungi in Yaoluoping Nature Reserve, Anhui. Journal of Resources and Ecology 7 (2): 144–150. https://doi.org/10.5814/j.issn.1674-764x.2016.02.010
Huang, Y., Wang, X., Feng, Z., Cui, H., Zhu, Z., Xia, C., Han, X., Liu, W. & Liu, Y. (2020) Cordyceps cicadae Prevents Renal Tubular Epithelial Cell Apoptosis by Regulating the SIRT1/p53 Pathway in Hypertensive Renal Injury. Evidence-Based Complementary and Alternative Medicine 2020: 1–13. https://doi.org/10.1155/2020/7202519
Hyde, K., Xu, J., Rapior, S., Jeewon, R., Lumyong, S., Niego, A.G., Abeywickrama, P., Vishvakeerthi, J., Brahmanage, R., Brooks, S., Chaiyasen, A., Chethana, K., Chomnunti, P., Chepkirui, C., Chuankid, B., de Silva, N., Doilom, M., Faulds, C., Gentekaki, E. & M. Stadler. (2019) The amazing potential of fungi: 50 ways we can exploit fungi industrially. Fungal Diversity 97: 1–136. https://doi.org/10.1007/s13225-019-00430-9
Jayasiri, S.C., Hyde, K.D., Ariyawansa, Bhat, J., Buyck, B., Cai, L., Dai, Y.C., Abd-Elsalam, K.A., Ertz, D., Hidayat, I., Jeewon, R., Jones, E.B.G., Bahkali, A.H., Karunarathna, S.C., Liu, J.K., Luangsa-Ard, J.J., Lumbsch, H.T., Maharachchikumbura, S.S.N., McKenzie, E.H.C., Moncalvo, J.M., Ghobad-Nejhad, M., Nilsson, H., Pang, K.L., Pereira, O.L., Phillips, A.J.L., Raspe, O. Rollins, A.W., Romero, A.I., Etayo, J., Selcuk, F., Stephenson, S.L., Suetrong, S., Taylor, J.E., Tsui, C.K.M., Vizzini, A., Abdel-Wahab, M.A., Wen, T.C., Boonmee, S., Dai, D.Q., Daranagama, D.A., Dissanayake, A.J., Ekanayaka, A.H., Fryar, S.C., Hongsanan, S., Jayawardena, R.S., Li, W.J., Perera, R.H., Phookamsak, R., De Silva, N.I., Thambugala, K.M., Tian, Q., Wijayawardene, N.N., Zhao, R.L., Zhao, Q., Kang, J.C. & Promputtha, I. (2015) The Faces of Fungi database: fungal names linked with morphology, phylogeny and human impacts. Fungal Diversity 74 (1): 3–18. https://doi.org/10.1007/s13225-015-0351-8
Katoh, K. & Standley, D.M. (2013) MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Molecular Biology and Evolution 30 (4): 772–780. https://doi.org/10.1093/molbev/mst010
Kepler, R.M., Luangsa-ard, J.J., Hywel-Jones, N.L., Quandt, C.A., Sung, G.H., Rehner, S.A., Aime, M.C., Henkel, T.W., Sanjuan, T., Zare, R., Chen, M.J., Li, Z.Z., Rossman, A.Y., Spatafora, J.W. & Shrestha, B. (2017) A phylogenetically-based nomenclature for Cordycipitaceae (Hypocreales). IMA Fungus 8 (2): 335–353. https://doi.org/10.5598/imafungus.2017.08.02.08 
Kepler, R.M., Sung, G.H., Ban, S., Nakagiri, A., Chen, M.J., Huang, B., Li, Z. & Spatafora, J.W. (2012) New teleomorph combinations in the entomopathogenic genus Metacordyceps. Mycologia 104 (1): 182–197. https://doi.org/10.3852/11-070
Kobayasi & Shimizu. (1982) Key to the taxa of the genera Cordyceps and Torrubiella. Transactions of the Mycological Society of Japan 23: 329–364.
Kobayasi, Y. (1941) The genus Cordyceps and its allies. Science reports of the Tokyo Bunrika Daigaku 5: 53–260.
Li, C.R., Zuo, D.P., Nam, S.H., Pu, S.C., Fan, M.Z. & Li, Z.Z. (2007) Cordyceps takaomontana and its anamorph Paecilomyces tenuipes. Mycosystema 26 (2): 217–220. https://doi.org/10.13346/j.mycosystema.2007.02.012
Li, L.Q., Song, A.X., Yin, J., Siu, K.C., Wong, W.T. & J. Wu. (2020) Anti-inflammation activity of exopolysaccharides produced by a medicinal fungus Cordyceps sinensis Cs-HK1 in cell and animal models. International Journal of Biological Macromolecules 149: 1042–1050. https://doi.org/10.1016/j.ijbiomac.2020.02.022
Li, T.H., Deng, C.Y. & Song, B. (2008) A distinct species of Cordyceps on coleopterous larvae hidden in twigs. Mycotaxon 103: 365–369.
Liang, Z., He, X., Han, Y., Cai, S. & Liang, J. (2008) A new species of Isaria isolated from an infected locust. Mycotaxon 105: 29–36.
Liang, Z.Q., Liu, A.Y., Liu, M.H. & Kang, J.C. (2003) The genus Cordyceps and its allies from the Kuankuoshui Reserve in Guizhou III. Fungal Diversity 14: 95–101.
Maharachchikumbura, S., Hyde, K., Jones, E., McKenzie, E., Bhat, D.J., Dayarathne, M., Huang, S.K., Norphanphoun, C., Senanayake, I., Perera, R., Shang, Q.J., Xiao, Y.P., D’souza, M., Hongsanan, S., Jayawardena, R., Daranagama, D., Konta, S., Goonasekara, I., Zhuang, W.Y. & Wijayawardene, N. (2016) Families of Sordariomycetes. Fungal Diversity 79: 1–317. https://doi.org/10.1007/s13225-016-0369-6.
Mains, E.B. (1958) North American Entomogenous Species of Cordyceps. Mycologia 50 (2): 169–222.
Miller, M., Pfeiffer, W.T. & Schwartz, T. (2010) Creating the CIPRES Science Gateway for Inference of Large Phylogenetic Trees. Vol. 14.
Mongkolsamrit, S., Noisripoom, W., Thanakitpipattana, D., Wutikhun, T., Spatafora, J.W. & Luangsa-ard, J.J. (2018) Disentangling cryptic species with Isaria-like morphs in Cordycipitaceae. Mycologia 110 (1): 230–257. https://doi.org/10.1080/00275514.2018.1446651
Mongkolsamrit, S., Noisripoom, W., Tasanathai, K., Khonsanit, A., Thanakitpipattana, D., Himaman, W., Kobmoo, N. & Luangsa-ard, J.J. (2020) Molecular phylogeny and morphology reveal cryptic species in Blackwellomyces and Cordyceps (Cordycipitaceae) from Thailand. Mycological Progress 19 (9): 957–983. https://doi.org/10.1007/s11557-020-01615-2
Mueller, G.M. & Schmit, J.P. (2007) Fungal biodiversity: what do we know? What can we predict? Biodiversity and Conservation 16 (1): 1–5.
Nylander, J. (2004) MrModeltest V2. Program Distributed by the Author. Bioinformatics 24: 581–583.
Rambaut, A.S., Xie, D., Drummond, A.J. (2013) Tracer version 1.6. University of Edinburgh. [Online]. [Accessed on 19.11.2016] available at. http://tree.bio.ed.ac.uk/software/tracer.
Ronquist, F., Teslenko, M., Van der, M.P., Ayres, D.L., Darling, A., Höhna, S., Larget, B., Liu, L., Suchard, M.A. & Huelsenbeck, J.P. (2012) MrBayes version 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology 61: 539–542. https://doi.org/10.1093/sysbio/sys029
Rossman, A.Y., Samuels, G.J., Rogerson, C.T. & R. Lowen. (1999) Genera of Bionectriaceae, Hypocreaceae and Nectriaceae (Hypocreales, Ascomycetes). Studies in Mycology 42: 1–248.
Saccardo, P.A. (1878) Enumeratio Pyrenomycetum Hypocreaceorum hucusque cognitorum systemate carpologico dispositorum. Michelia 1: 277–325.
Samson, R. (1974) Paecilomyces and some allied Hyphomycetes. Studies in Mycology 6.
Samson, R., Evans, H.C. & Latgé, J.P. (1988) Atlas of Entomopathogenic Fungi. Vol. 82. https://doi.org/10.1007/978-3-662-05890-9
Schoch, C.L., Seifert, K.A., Huhndorf, S., Robert, V., Spouge, J.L., Levesque, C.A., Chen, W., Bergeron, M.J., Hamelin, R.C., Vialle, A. & Fungal Barcoding Consortium. (2012) Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi. Proceedings of the National Academy of Science 109: 6241–6246. https://doi.org/10.1073/pnas.1117018109
Shrestha, B., Tanaka, E., Hyun, M.W., Han, J., Kim, C.S., Jo, J.W., Han, S., Oh, J. & Sung, G. (2016) Coleopteran and Lepidopteran Hosts of the Entomopathogenic Genus Cordyceps sensu lato. Journal of Mycology 2016 (2): 1–14. https://doi.org/10.1155/2016/7648219
Song, B., Lin, Q.Y., Li, T.H., Shen, Y.H., Li, J.J. & Luo, D.X. (2006) Known species of Cordyceps from China and their distribution. Journal of Fungal Research 4 (4): 10–26.
Stamatakis, A. (2014) RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 30: 1312–1313. https://doi.org/10.1093/bioinformatics/btu033
Sung, G.H., Hywel-Jones, N.L., Sung, J.M., Luangsa-ard, J.J., Shrestha, B. & Spatafora, J.W. (2007a) Phylogenetic classification of Cordyceps and the clavicipitaceous fungi. Studies in Mycology 57: 5–59. https://doi.org/10.3114/sim.2007.57.01
Sung, G.H., Sung, J.M., Hywel-Jones, N.L. & Spatafora, J.W. (2007b) A multi-gene phylogeny of Clavicipitaceae (Ascomycota, Fungi): identification of localized incongruence using a combinational bootstrap approach. Molecular Phylogenetics and Evolution 44 (3): 1204–1223. https://doi.org/10.1016/j.ympev.2007.03.011
Tasanathai, K., Thanakitpipattana, D., Noisripoom, W., Khonsanit, A., Kumsao, J. & Luangsa-ard, J.J. (2016) Two new Cordyceps species from a community forest in Thailand. Mycological Progress 15 (3): 28. https://doi.org/10.1007/s11557-016-1170-3
Teng, S.C. (1934) Notes on Hypocreales from China. Sinensia 4: 269–299.
Vilgalys, R. & Hester, M. (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. Journal of Bacteriology 172 (8): 4238–4246. https://doi.org/10.1128/jb.172.8.4238-4246.1990
Wang, S.B., Bao, B.F., Fan, M.Z. & Li, Z.Z. (2003) Community structure and species diversity of insect-borne fungi in the Yaoluoping National Nature Reserve. Chinese Journal of Applied Ecology 20 (9): 2262. https://doi.org/10.1007/978-3-642-02298-2_32
Wang, Y.B., Wang, Y., Fan, Q., Duan, D.E., Zhang, G.D., Dai, R.Q., Dai, Y.D., Zeng, W.B., Chen, Z.H., Li, D.D., Tang, D.X., Xu, Z.H., Sun, T., Nguyen, T.T., Tran, N.L., Dao, V.M., Zhang, C.M., Huang, L.D., Liu, Y.J., Zhang, X.M., Yang, D.R., Sanjuan, T., Liu, X.Z., Yang, Z.L. & Yu, H. (2020) Multigene phylogeny of the family Cordycipitaceae (Hypocreales): new taxa and the new systematic position of the Chinese cordycipitoid fungus Paecilomyces hepiali. Fungal Diversity 103 (1): 1–46. https://doi.org/10.1007/s13225-020-00457-3
White, T.J., Bruns, T., Lee, S. & Taylor, J. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics: PCR-protocols and applications-A laboratory manual. PCR Protocols: A Guide to Methods and Applications 315–322. https://doi.org/10.1016/B978-0-12-372180-8.50042-1
Wijayawardene, N.N., Hyde, K.D., Rajeshkumar, K.C., Hawksworth, D.L., Madrid, H., Kirk, P.M., Braun, U., Singh, R.V., Crous, P.W., Kukwa, M., Lücking, R., Kurtzman, C.P., Yurkov, A., Haelewaters, D., Aptroot, A., Lumbsch, H.T., Timdal, E., Ertz, D., Etayo, J., Phillips, A.J.L., Groenewald, J.Z., Papizadeh, M., Selbmann, L., Dayarathne, M.C., Weerakoon, G., Jones, E.B.G., Suetrong, S., Tian, Q., Castañeda-Ruiz, R.F., Bahkali, A.H., Pang, K.-L., Tanaka, K., Dai, D.Q., Sakayaroj, J. Hujslová, M., Lombard, L., Shenoy, B.D., Suija, A., Maharachchikumbura, S.S.N., Thambugala, K.M., Wanasinghe, D.N., Sharma, B.O., Gaikwad, S., Pandit, G., Zucconi, L., Onofri, S., Egidi, E., Raja, H.A., Kodsueb, R., Cáceres, M.E.S., Pérez-Ortega, S., Fiuza, P.O., Monteiro, J.S., Vasilyeva, L.N., Shivas, R.G., Prieto, M., Wedin, M., Olariaga, I., Lateef, A.A., Agrawal, Y., Fazeli, S.A.S., Amoozegar, M.A., Zhao, G.Z., Pfliegler, W.P., Sharma, G., Oset, M., Abdel-Wahab, M.A., Takamatsu, S., Bensch, K., de Silva, N.I., De Kesel, A., Karunarathna, A., Boonmee, S., Pfister, D.H., Lu, Y.Z., Luo, Z.L., Boonyuen, N., Daranagama, D.A., Senanayake, I.C., Jayasiri, S.C., Samarakoon, M.C., Zeng, X.Y., Doilom, M., Quijada, L., Rampadarath, S., Heredia, G., Dissanayake, A.J., Jayawardana, R.S., Perera, R.H., Tang, L.Z., Phukhamsakda, C., Hernández-Restrepo, M., Ma, X., T., Saowaluck, G., L.F.P., Weerahewa, D. & Karunarathna, S.C. (2017) Notes for genera: Ascomycota. Fungal Diversity 86: 1–594. https://doi.org/10.1007/s13225-017-0386-0
Xiang, X.Y., Wu, G.L., Lu, K.Q., Yang, J.X., Bao, X. & Shen, S.Q. (2013) Community distribution of macrofungi in Yaoluoping Nature Reserve. Guihaia 33 (02): 258–262.
Xie, Z. & Wu, K. (1995) The Vegetation Types and Their Distributions In Yaoluoping Natural Reserve of Anhui Province. Journal of East China Normal University (Natural Science) 03: 93–100.
Yan, J.Q. & Bau, T. (2015) Cordyceps ningxiaensis sp. nov., a new species from dipteran pupae in Ningxia Hui Autonomous Region of China. Nova Hedwigia 100 (1): 251–258. https://doi.org/10.1127/nova_hedwigia/2014/0222
Yang, Y., Cai, S., Zheng, Y., Lu, X., Xu, X. & Han, Y. (2009) Metarhizium taii var. chongqingensis nov., anamorph of Cordyceps chongqingensis sp. nov. isolated from a low altitude area in Chongqing, China. Current Microbiology 58 (6): 635. https://doi.org/10.1007/s00284-009-9382-2
Zimmermann, G. (2007) Review on safety of the entomopathogenic fungi Beauveria bassiana and Beauveria brongniartii. Biocontrol Science and Technology 17: 553–596. https://doi.org/10.1080/09583150701309006