Skip to main content Skip to main navigation menu Skip to site footer
Type: Article
Published: 2022-07-28
Page range: 291-300
Abstract views: 63
PDF downloaded: 2

Lepiota cremea, a novel species of Lepiota sect. Lepiota (Agaricaceae s.l., Agaricales) from Turkey

Department of Plant and Animal Production, Atabey Vocational School, Isparta University of Applied Sciences, Isparta – 32670, Turkey
Agaricomycetes Basidiomycota Denizli ITS phylogeny taxonomy Fungi


A new species of Lepiota from Turkey, Lepiota cremea sp. nov., is proposed based on morphological characters and phylogenetic analyses of the nuclear rDNA internal transcribed spacer (ITS1-5.8S-ITS2 = ITS) region. The morphological features together with molecular data confirmed both the novelty of this species and its infrageneric placement within Lepiota sect. Lepiota. Lepiota cremea, so far only found in Pinus nigra subsp. pallasiana forest, is characterized by the pale beige basidiomata, subcylindrical to fusiform basidiospores, long pileus covering elements with often nodulose or horn-like bumps. A comprehensive description, illustrations and comparisons with morphologically similar and related taxa are provided.


<p>Babos, M. (1974) Studies on Hungarian <em>Lepiota</em> s.l. species, IV. <em>Annales Historico Naturales Musei Nationali Hungarici</em> 66: 65–75.</p>
<p>Birkebak, J.M., Vellinga, E.C., Franco-Molano, A.E., Wood, M.G. &amp; Matheny, P.B. (2011) <em>Lepiota maculans</em>, an unusual mushroom rediscovered after 105 years. <em>Southeastern Naturalist</em> 10 (2): 267–274.</p>
<p>Bon, M. (1980) (‘1979’) Novitates: Taxons nouveaux. <em>Documents Mycologiques</em> 10 (37–38): 89–92.</p>
<p>Bon, M. (1993) Famille Lepiotaceae Roze ex Overeem. <em>Documents Mycologiques</em> 22 (88): 27–32.</p>
<p>Bresadola, G. (1881) <em>Fungi Tridentini novi vel nondum delineati descripti et iconibus illustrati</em>. Vol. 1. J.B. Monauni. Tridenti, Trento, 346 pp.</p>
<p>Caballero, A., Vizzini, A., Muñoz, G., Contu, M. &amp; Ercole, E. (2015) <em>Lepiota elseae</em> (Agaricales, Agaricaceae), a new species of section <em>Lepiota</em> from Spain. <em>Phytotaxa</em> 201 (3): 188–196. &nbsp;</p>
<p>Candusso, M. &amp; Lanzoni, G. (1990) <em>Lepiota</em> <em>s.l.</em> <em>Fungi europaei 4</em>. Giovanna Biella, Saronno, 743 pp.</p>
<p>Edgar, R.C. (2004) MUSCLE: multiple sequence alignment with high accuracy and high throughput. <em>Nucleic Acids Research</em> 32: 1792–1797.&nbsp;</p>
<p>Gardes, M. &amp; Bruns, T.D. (1993) ITS primers with enhanced specificity for Basidiomycetes application to the identification of mycorrhizae and rusts. <em>Molecular Ecology</em> 2: 113–118.&nbsp;</p>
<p>Ge, Z-W. &amp; Smith, M.E. (2013) Phylogenetic analysis of rDNA sequences indicates that the sequestrate <em>Amogaster viridiglebus</em> is derived from within the agaricoid genus <em>Lepiota</em> (Agaricaceae). <em>Mycological Progress</em> 12 (1): 151–155.&nbsp;</p>
<p>Gillet, C.G. (1874) <em>Les Hyménomycètes, ou, Description de tous les champignons (fungi): qui croissent en France, avec l’indication de leurs propriétés utiles ou vénéneuses</em>. Ch. Thomas, Alençon, France, 517 pp.</p>
<p>Gray, S.F. (1821) <em>A natural arrangement of British plants, according to their relations to each other, as pointed out by Jussieu, De Candolle, Brown, &amp;c. including those cultivated for use; with an introduction to botany, in which the terms newly introduced are explained; illustrated by figures</em>. vol. 1. Cradock &amp; Joy, Paternoster-Row, London, Baldwin. pp. [i]–xxviii, [1]–824, pls I–XXI plates.</p>
<p>Hall, T.A. (1999) BioEdit: A user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. <em>Nucleic Acids Symposium Series</em> 41: 95–98.&nbsp;</p>
<p>Hou, Y.J. &amp; Ge, Z.W. (2020) New species of <em>Echinoderma</em> and <em>Lepiota</em> (Agaricaceae) from China. <em>Phytotaxa</em> 447 (4): 221–236.&nbsp;</p>
<p>Hughes, K.W., Petersen, R.H. &amp; Lickey, E.B. (2009) Using heterozygosity to estimate a percentage DNA sequence similarity for environmental species’ delimitation across basidiomycete fungi. <em>New Phytologist</em> 182 (2009): 795–798.&nbsp;</p>
<p>Johnson, J. (1999) Phylogenetic relationships within <em>Lepiota</em> sensu lato based on morphological and molecular data. <em>Mycologia</em> 91 (3): 443–458.</p>
<p>Justo, A., Angelini, C. &amp; Bizzi, A. (2015) Two new species and a new record of <em>Lepiota</em> (Basidiomycota, Agaricales) from the Dominican Republic. <em>Mycological Progress</em> 14: 56.</p>
<p>Katoh, K. &amp; Standley, D.M. (2013) MAFFT multiple sequence alignment software version 7: improvements in performance and usability. <em>Molecular Biology and Evolution</em> 30: 772–780.&nbsp;</p>
<p>Kaygusuz, O., Justo, A., Knudsen, H., Ševčíková, H. &amp; İbrahim, T. (2021) <em>Pluteus lauracearum</em> (Agaricales, Basidiomycota), a new species of <em>Pluteus</em> sect. <em>Hispidoderma</em> from thermophilic <em>Laurus</em> forests. <em>Phytotaxa</em> 523 (2): 126–140.&nbsp;</p>
<p>Kirk, P.M., Cannon, P.F., Minter, D.W. &amp; Stalpers, J.A. (2008) <em>Ainsworth &amp; Bisby’s Dictionary of the Fungi. 10th ed.</em> CABI Publishing, Wallingford, UK, 771 pp.</p>
<p>Kropp, B.R., Albee-Scott, S., Castellano, M. &amp; Trappe, J.M. (2012) <em>Cryptolepiota</em>, a new sequestrate genus in the <em>Agaricaceae</em> with evidence for adaptive radiation in western North America. <em>Mycologia</em> 104: 164–174.&nbsp;</p>
<p>Kummer, P. (1871) <em>Der Führer in die Pilzkunde. Edn. 1.</em> E. Luppe, Zerbst, 146 pp.</p>
<p>Lebel, T. &amp; Vellinga, E.C. (2013) Description and affinities of a sequestrate <em>Lepiota</em> (Agaricaceae) from Australia. <em>Mycological Progress</em> 12: 525–532.&nbsp;</p>
<p>Liang, J.F. &amp; Yang, Z.L. (2011) A new species of <em>Lepiota</em> (Agaricaceae) from southwestern China. <em>Mycotaxon</em> 117: 359–363.</p>
<p>Liang, J.F., Yang, Z.L. &amp; Xu, D.P. (2011) A new species of <em>Lepiota</em> from China. <em>Mycologia</em> 103: 820–830.&nbsp;</p>
<p>Liang, J.F., Yang, Z.L., Xu, J. &amp; Ge, Z.W. (2010) Two new unusual <em>Leucoagaricus</em> species (Agaricaceae) from tropical China with blue-green staining reactions. <em>Mycologia</em> 102: 1141–1152.&nbsp;</p>
<p>Liang, J.F., Yu, F., Lu, J.K., Wang, S.K. &amp; Song, J. (2018) Morphological and molecular evidence for two new species in <em>Lepiota</em> from China. <em>Mycologia</em> 110: 494–501.&nbsp;</p>
<p>Linnaeus, C. (1753) <em>Species Plantarum, vols. 1–2</em>. Impensis Laurentii Salvii, Holmiæ [Stockholm], 1200 pp.</p>
<p>Locquin, M. (1956) Quelques Lepiotes nouvelles ou critiques, <em>Lepiota helveola</em> Bres. ss. Josserand, <em>L. Sericifera</em> (Locq.) Locq. et <em>L. Kühneriana</em> sp. nov. <em>Friesia</em> 5 (3/5): 293–296.</p>
<p>Long, P., Fan, F., Xu, B., He, Z., Su, Y., Zhang, P., Xie, J. &amp; Chen, Z. (2020) Determination of Amatoxins in <em>Lepiota brunneoincarnata</em> and <em>Lepiota venenata</em> by High-Performance Liquid Chromatography Coupled with Mass Spectrometry. <em>Mycobiology</em> 48 (3): 204–209.&nbsp;</p>
<p>Massee, G. (1898) Fungi Exotici 1. <em>Bulletin of miscellaneous information (Royal Botanic Gardens, Kew)</em> 1898 113–136.</p>
<p>Miller, M.A., Pfeiffer, W. &amp; Schwartz, T. (2010) <em>Creating the CIPRES Science Gateway for inference of large phylogenetic trees.</em> Proceedings of the Gateway Computing Environments Work shop (GCE). New Orleans, USA: GCE; pp. 1–8.&nbsp;</p>
<p>Muhire, B.M., Arvind, V. &amp; Darren, P.M. (2014) SDT: a virus classification tool based on pairwise sequence alignment and identity calculation. <em>PLOS One</em> 9 (9): e108277.</p>
<p>Nawaz, R., Khalid, A.N., Hanif, M. &amp; Razaq, A. (2013) <em>Lepiota vellingana</em> sp. nov. (Basidiomycota, Agaricales) a new species from Lahore, Pakistan. <em>Mycological Progress</em> 12: 727–732.&nbsp;</p>
<p>Nylander, J.A.A. (2004) <em>MrModeltest version 2. Program distributed by the author</em>. Uppsala, Sweden: Uppsala University, Evolutionary Biology Centre.</p>
<p>Rambaut, A. (2016) FigTree v1.4.3: Tree figure drawing tool. Available from: (accessed 28 July 2022)</p>
<p>Razaq, A., Khalid, A.N. &amp; Vellinga, E.C. (2013) <em>Lepiota himalayensis</em> (Basidiomycota, Agaricales), a new species from Pakistan. <em>Mycotaxon</em> 121: 319–325.&nbsp;</p>
<p>Ronquist, F., Teslenko, M., van der Mark, P., Ayres, D.L., Darling, A., Höhna, S., Larget, B., Liu, L., Suchard, M.A. &amp; Huelsenbeck, J.P. (2012) MrBayes 3.2: Efficient Bayesian phylogenetic inference and model choice across a large model space. <em>Systematic Biology </em>61: 589–542.</p>
<p>Saccardo, P.A. (1887) Sylloge Hymenomycetum, vol. 1. Agaricineae. <em>Sylloge Fungorum</em> 5: 1–1146.</p>
<p>Sesli, E., Asan, A. &amp; Selçuk, F. (2020) <em>The Checklist of Fungi of Turkey (Türkiye Mantarları Listesi)</em>. Ali Nihat Gökyiğit Vakfı Yayını, İstanbul, Turkey, 1177 pp.</p>
<p>Singer, R. &amp; Digilio, A.P.L. (1952) (‘1951’) Pródromo de la Flora Agaricina Argentina. <em>Lilloa</em> 25: 5–461.</p>
<p>Singer, R. (1986) <em>Lepiota</em> (Pers. ex) S.F. Gray. <em>In:</em> Singer, R. (Ed.) <em>The Agaricales in modern taxonomy</em>, 4<sup>th</sup> Edition. Koeltz Scientific Books, Koenigstein, Germany, pp. 497–501.</p>
<p>Solak, M.H. &amp; Türkoğlu, A. (2022) <em>Macrofungi of Turkey, Checklist vol. III</em>. Kanyilmaz Matbaacilik Kagit ve Ambalaj Sanayi Ticaret Ltd. Sti, İzmir, Turkey, 426 pp.</p>
<p>Söylemez, T. &amp; Yamaç, M. (2021) Screening of Macrofungi Isolates for Aflatoxin B1 and Ochratoxin A Degradation. <em>Biol Bull Russ Acad Sci</em> 48: 122–129.&nbsp;</p>
<p>Stamatakis, A. (2014) RAxML version 8: A tool for Phylogenetic Analysis and Post-Analysis of Large Phylogenies. <em>Bioinformatics</em> 30: 1312–1313.&nbsp;</p>
<p>Sysouphanthong, P., Guo, J.Y., Hyde, K.D., Xu, J.C. &amp; Mortimer, P.E. (2016) <em>Lepiota thailandica</em> (Agaricaceae), a new species from Thailand. <em>Phytotaxa</em> 245 (4): 262–270.&nbsp;</p>
<p>Sysouphanthong, P., Hyde, K.D., Chukeatirote, E., Bahkali, A.H. &amp; Vellinga, E.C. (2011) <em>Lepiota</em> (Agaricales) in northern Thailand 1 <em>Lepiota</em> section <em>Stenosporae</em>. <em>Mycotaxon</em> 117: 53–85.&nbsp;</p>
<p>Sysouphanthong, P., Hyde, K.D., Chukeatirote, E., Bahkali, A.H. &amp; Vellinga, E.C. (2012) <em>Lepiota</em> (Agaricales) in northern Thailand 2 <em>Lepiota</em> section <em>Lepiota</em>. <em>Cryptogamie Mycologie</em> 33: 25–42.</p>
<p>Velenovskı, J. (1920) <em>České Houby 1.</em> České Botanické Společnosti, Praze, 133 pp.</p>
<p>Velenovskı, J. (1921) <em>České Houby</em> <em>3</em>. České Botanické Společnosti, Praha, pp. 425–632.</p>
<p>Vellinga, E.C. &amp; Huijser, H.A. (1999) Studies in <em>Lepiota</em> I. Species with a hymeniform pileus covering. <em>Belgian Journal of Botany</em> 131: 191–210.</p>
<p>Vellinga, E.C. (1988) Glossary. <em>In</em>: Bas, C., Kuyper, Th.W., Noordeloos, M.E. &amp; Vellinga, E.C. (Eds.) <em>Flora Agaricina Neerlandica, vol. I</em>. A.A. Balkema Publishers, Rotterdam, pp. 54–64.</p>
<p>Vellinga, E.C. (2001a) Studies in <em>Lepiota</em> III. Some species from California. <em>Mycotaxon</em> 80: 285–295.&nbsp;</p>
<p>Vellinga, E.C. (2001b) <em>Lepiota</em>. <em>In:</em> Noordeloos, M.E., Kuyper, Th.W. &amp; Vellinga, E.C. (Eds.) <em>Flora Agaricina Neerlandica, vol. V.</em> A.A. Balkema Publishers, Rotterdam, pp. 109–151.</p>
<p>Vellinga, E.C. (2001c) Studies in <em>Lepiota</em> IV. <em>Lepiota cristata</em> and <em>L. castaneidisca</em>. <em>Mycotaxon</em> 80: 297–306.</p>
<p>Vellinga, E.C. (2003) Phylogeny of <em>Lepiota</em> (Agaricaceae) evidence from nrITS and nrLSU sequences. <em>Mycological Progress </em>2: 305–322.&nbsp;</p>
<p>Vellinga, E.C. (2004) Genera in the family Agaricaceae: evidence from nrITS and nrLSU sequences. <em>Mycological Research</em> 108: 354–377.&nbsp;</p>
<p>Vellinga, E.C. (2006a) Lepiotaceous fungi in California, U.S.A. 4. Type studies of <em>Lepiota fumosifolia</em> and <em>L. petasiformis</em>. <em>Mycotaxon</em> 98: 225–232.</p>
<p>Vellinga, E.C. (2006b) Lepiotaceous fungi in California, U.S.A. – 2. <em>Lepiota rhodophylla</em> sp. nov. <em>Mycotaxon</em> 98: 205–211.</p>
<p>Vellinga, E.C. (2010) <em>Lepiota</em> in California: species with a hymeniform pileus covering. <em>Mycologia</em> 102: 664–674.&nbsp;</p>
<p>Vidal, J.M., Juste, P., García, F., Bellanger, J.M. &amp; Moreau, P.A. (2015) Hongos secotioides del género <em>Lepiota</em>: dos nuevas especies, dos nuevas combinaciones y reevaluación del género <em>Cribrospora</em>. <em>Boletín Micológico de FAMCAL</em> 10: 47–71.</p>
<p>Wasser, S.P. (1978) <em>Leucocoprinus</em> Pat. (Agaricales Clem.) in URSS. Novitates Systematicae Plantarum Vascularium et non Vascularium. <em>Acad. Sci. RSS UCR</em> 5: 207–225.</p>
<p>White, T.J., Bruns, T., Lee, S. &amp; Taylor, J. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. <em>In</em>: Innis, M.A., Gelfand, D.H., Sninsky, J.J. &amp; White, T.J. (Eds.) <em>PCR Protocols: A Guide to Methods and Applications</em>. Academic Press, New York, pp. 315–322.&nbsp;</p>