Abstract
A new species, Bredia bullata, from China, is described and illustrated based on morphological and molecular evidence. This new species is morphologically similar to B. malipoensis in having tomentose stems and leaves, cordate leaf blades in unequal pairs, an umbellate inflorescence, and undulate petals with an unguiculate base and retuse apex, but differs by having bullate leaf blades, white hypanthia and elliptic calyx lobes. Our phylogenetic analysis using the plastid genome strongly supports the generic placement of B. bullata and B. malipoensis within Bredia.
References
<p>Blume, C.L. (1849) Melastomataceae. <em>Museum Botanicum Lugduno-Batavum</em> 1: 1–25.</p>
<p>Capella-Gutiérrez, S., Silla-Martínez, J.M. & Gabaldón, T. (2009) trimAl: a tool for automated alignment trimming in large-scale phylogenetic analyses. <em>Bioinformatics</em> 25: 1972–1973. https://doi.org/10.1093/bioinformatics/btp348</p>
<p>Chen, C. (1984) Melastomataceae. <em>In: </em>Chen, C. (Ed.) <em>Flora Reipublicae Popularis Sinicae vol. 53</em>. Science Press, Beijing, pp. 135–293.</p>
<p>Chen, C. & Renner, S.S. (2007) Melastomataceae. <em>In:</em> Wu, Z.Y., Raven, P.H. & Hong, D.Y. (Eds.) <em>Flora of China vol. 13</em>. Science Press, Beijing, and Missouri Botanical Garden Press, St. Louis, pp. 377–384.</p>
<p>Dai, J.H., Zhou, Q.J., Zhou, R.C. & Liu, Y. (2020) A new species of <em>Bredia</em> (Sonerileae, Melastomataceae) from Sichuan, China. <em>Phytokeys</em> 152: 1–14. https://doi.org/10.3897/phytokeys.152.53512</p>
<p>Diels, F.L.E. (1932) Beiträge zur kenntnis der Melastomataceen ostasiens. <em>Botanische Jahrbücher für Systematik</em> 65: 97–119.</p>
<p>He, C.M., Zeng, S.J., Li, S.W. & Tong, Y.H. (2020) <em>Bredia reniformis</em> (Melastomataceae), a new species from Guangxi, China. <em>Nordic Journal of Botany</em> 38: e02525. https://doi.org/10.1111/njb.02525</p>
<p>Hoang, D.T., Chernomor, O., von Haeseler, A., Minh, B.Q. & Vinh, L.S. (2018) UFBoot2: improving the ultrafast bootstrap approximation. <em>Molecular Biology and Evolution</em> 35: 518–522. https://doi.org/10.1093/molbev/msx281</p>
<p>Jin, J.J., Yu, W.B., Yang, J.B., Song, Y., dePamphilis, C.W., Yi, T.S. & Li, D.Z. (2020) GetOrganelle: a fast and versatile toolkit for accurate de novo assembly of organelle genomes. <em>Genome Biology </em>21: 241. https://doi.org/10.1186/s13059-020-02154-5</p>
<p>Katoh, K. & Standley, D.M. (2013) MAFFT Multiple Sequence Alignment Software Version 7: improvements in performance and usability. <em>Molecular Biology and Evolution</em> 30: 772–780. https://doi.org/10.1093/molbev/mst010</p>
<p>Loureiro, J. (1790) <em>Flora Cochinchinensis vol. 2</em>. Ulyssipone, Lisbon, 387 pp.</p>
<p>Nguyen, L.T., Schmidt, H.A., von Haeseler, A. & Minh, B.Q. (2015) IQ-TREE: a fast and effective stochastic algorithm for estimating maximum-likelihood phylogenies. <em>Molecular Biology and Evolution</em> 32: 268–274. https://doi.org/10.1093/molbev/msu300</p>
<p>Pham, H.H. (2000) <em>An Illustrated Flora of Vietnam vol. 2</em>. Young Publishing House, Hanoi, 951 pp.</p>
<p>Qu, X.J., Moore, M.J., Li, D.Z. & Yi, T.S. (2019) PGA: a software package for rapid, accurate, and flexible batch annotation of plastomes. <em>Plant Methods</em> 15: 50. https://doi.org/10.1186/s13007-019-0435-7</p>
<p>Stapf, O. (1892) On the Sonerileae of Asia. <em>Annals of Botany</em> 6: 291–323. https://doi.org/10.1093/oxfordjournals.aob.a090681</p>
<p>Wen, Z.Y., Zeng, S.J., Fan, W.L., Zhang, G.Q. & Peng, D.H. (2019) <em>Bredia malipoensis</em> (Melastomataceae), a new species from Yunnan, China. <em>Phytotaxa</em> 425: 127–136. https://doi.org/10.11646/phytotaxa.425.3.2</p>
<p>Zeng, S.J., Zou, L.H., Wang, P., Hong, W.J., Zhang, G.Q., Chen, L.J. & Zhuang, X.Y. (2016) Preliminary phylogeny of <em>Fordiophyton</em> (Melastomataceae), with the description of two new species. <em>Phytotaxa</em> 247: 45–61. https://doi.org/10.11646/phytotaxa.247.1.3</p>
<p>Zhao, W.Y., Fan, Q., Ye, H.G., Zhan, X.H. & Liao, W.B. (2017) <em>Bredia changii</em>, a new species of Melastomataceae from Jiangxi, China. <em>Phytotaxa</em> 307: 36–42. https://doi.org/10.11646/phytotaxa.307.1.3</p>
<p>Zhou, Q.J., Dai, J.H., Lin, C.W., Denda, T., Zhou, R.C. & Liu, Y. (2019a) Recircumscription of <em>Bredia</em> and resurrection of <em>Tashiroea</em> (Sonerileae, Melastomataceae) with description of a new species <em>T. villosa</em>. <em>Phytokeys</em> 127: 121–150. https://doi.org/10.3897/phytokeys.127.36608</p>
<p>Zhou, Q.J., Lin, C.W., Dai, J.H., Zhou, R.C. & Liu, Y. (2019b) Exploring the generic delimitation of <em>Phyllagathis</em> and <em>Bredia</em> (Melastomataceae): a combined nuclear and chloroplast DNA analysis. <em>Journal of Systematics and Evolution</em> 57: 256–267. https://doi.org/10.1111/jse.12451</p>
<p>Zhou, Q.J., Lin, C.W., Ng, W.L., Dai, J.H., Denda, T., Zhou, R.C. & Liu, Y. (2019c) Analyses of plastome sequences improve phylogenetic resolution and provide new insight into the evolutionary history of Asian Sonerileae/Dissochaeteae. <em>Frontiers in Plant Science</em> 10: 1477. https://doi.org/10.3389/fpls.2019.01477</p>
<p>Zhou, R.C., Zhou, Q.J. & Liu, Y. (2018) <em>Bredia repens</em> (Melastomataceae), a new species from Hunan, China. <em>Systematic Botany</em> 43: 544–551. https://doi.org/10.1600/036364418X697265</p>
<p>Capella-Gutiérrez, S., Silla-Martínez, J.M. & Gabaldón, T. (2009) trimAl: a tool for automated alignment trimming in large-scale phylogenetic analyses. <em>Bioinformatics</em> 25: 1972–1973. https://doi.org/10.1093/bioinformatics/btp348</p>
<p>Chen, C. (1984) Melastomataceae. <em>In: </em>Chen, C. (Ed.) <em>Flora Reipublicae Popularis Sinicae vol. 53</em>. Science Press, Beijing, pp. 135–293.</p>
<p>Chen, C. & Renner, S.S. (2007) Melastomataceae. <em>In:</em> Wu, Z.Y., Raven, P.H. & Hong, D.Y. (Eds.) <em>Flora of China vol. 13</em>. Science Press, Beijing, and Missouri Botanical Garden Press, St. Louis, pp. 377–384.</p>
<p>Dai, J.H., Zhou, Q.J., Zhou, R.C. & Liu, Y. (2020) A new species of <em>Bredia</em> (Sonerileae, Melastomataceae) from Sichuan, China. <em>Phytokeys</em> 152: 1–14. https://doi.org/10.3897/phytokeys.152.53512</p>
<p>Diels, F.L.E. (1932) Beiträge zur kenntnis der Melastomataceen ostasiens. <em>Botanische Jahrbücher für Systematik</em> 65: 97–119.</p>
<p>He, C.M., Zeng, S.J., Li, S.W. & Tong, Y.H. (2020) <em>Bredia reniformis</em> (Melastomataceae), a new species from Guangxi, China. <em>Nordic Journal of Botany</em> 38: e02525. https://doi.org/10.1111/njb.02525</p>
<p>Hoang, D.T., Chernomor, O., von Haeseler, A., Minh, B.Q. & Vinh, L.S. (2018) UFBoot2: improving the ultrafast bootstrap approximation. <em>Molecular Biology and Evolution</em> 35: 518–522. https://doi.org/10.1093/molbev/msx281</p>
<p>Jin, J.J., Yu, W.B., Yang, J.B., Song, Y., dePamphilis, C.W., Yi, T.S. & Li, D.Z. (2020) GetOrganelle: a fast and versatile toolkit for accurate de novo assembly of organelle genomes. <em>Genome Biology </em>21: 241. https://doi.org/10.1186/s13059-020-02154-5</p>
<p>Katoh, K. & Standley, D.M. (2013) MAFFT Multiple Sequence Alignment Software Version 7: improvements in performance and usability. <em>Molecular Biology and Evolution</em> 30: 772–780. https://doi.org/10.1093/molbev/mst010</p>
<p>Loureiro, J. (1790) <em>Flora Cochinchinensis vol. 2</em>. Ulyssipone, Lisbon, 387 pp.</p>
<p>Nguyen, L.T., Schmidt, H.A., von Haeseler, A. & Minh, B.Q. (2015) IQ-TREE: a fast and effective stochastic algorithm for estimating maximum-likelihood phylogenies. <em>Molecular Biology and Evolution</em> 32: 268–274. https://doi.org/10.1093/molbev/msu300</p>
<p>Pham, H.H. (2000) <em>An Illustrated Flora of Vietnam vol. 2</em>. Young Publishing House, Hanoi, 951 pp.</p>
<p>Qu, X.J., Moore, M.J., Li, D.Z. & Yi, T.S. (2019) PGA: a software package for rapid, accurate, and flexible batch annotation of plastomes. <em>Plant Methods</em> 15: 50. https://doi.org/10.1186/s13007-019-0435-7</p>
<p>Stapf, O. (1892) On the Sonerileae of Asia. <em>Annals of Botany</em> 6: 291–323. https://doi.org/10.1093/oxfordjournals.aob.a090681</p>
<p>Wen, Z.Y., Zeng, S.J., Fan, W.L., Zhang, G.Q. & Peng, D.H. (2019) <em>Bredia malipoensis</em> (Melastomataceae), a new species from Yunnan, China. <em>Phytotaxa</em> 425: 127–136. https://doi.org/10.11646/phytotaxa.425.3.2</p>
<p>Zeng, S.J., Zou, L.H., Wang, P., Hong, W.J., Zhang, G.Q., Chen, L.J. & Zhuang, X.Y. (2016) Preliminary phylogeny of <em>Fordiophyton</em> (Melastomataceae), with the description of two new species. <em>Phytotaxa</em> 247: 45–61. https://doi.org/10.11646/phytotaxa.247.1.3</p>
<p>Zhao, W.Y., Fan, Q., Ye, H.G., Zhan, X.H. & Liao, W.B. (2017) <em>Bredia changii</em>, a new species of Melastomataceae from Jiangxi, China. <em>Phytotaxa</em> 307: 36–42. https://doi.org/10.11646/phytotaxa.307.1.3</p>
<p>Zhou, Q.J., Dai, J.H., Lin, C.W., Denda, T., Zhou, R.C. & Liu, Y. (2019a) Recircumscription of <em>Bredia</em> and resurrection of <em>Tashiroea</em> (Sonerileae, Melastomataceae) with description of a new species <em>T. villosa</em>. <em>Phytokeys</em> 127: 121–150. https://doi.org/10.3897/phytokeys.127.36608</p>
<p>Zhou, Q.J., Lin, C.W., Dai, J.H., Zhou, R.C. & Liu, Y. (2019b) Exploring the generic delimitation of <em>Phyllagathis</em> and <em>Bredia</em> (Melastomataceae): a combined nuclear and chloroplast DNA analysis. <em>Journal of Systematics and Evolution</em> 57: 256–267. https://doi.org/10.1111/jse.12451</p>
<p>Zhou, Q.J., Lin, C.W., Ng, W.L., Dai, J.H., Denda, T., Zhou, R.C. & Liu, Y. (2019c) Analyses of plastome sequences improve phylogenetic resolution and provide new insight into the evolutionary history of Asian Sonerileae/Dissochaeteae. <em>Frontiers in Plant Science</em> 10: 1477. https://doi.org/10.3389/fpls.2019.01477</p>
<p>Zhou, R.C., Zhou, Q.J. & Liu, Y. (2018) <em>Bredia repens</em> (Melastomataceae), a new species from Hunan, China. <em>Systematic Botany</em> 43: 544–551. https://doi.org/10.1600/036364418X697265</p>