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Type: Article
Published: 2021-07-06
Page range: 135–147
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Pinocchia daecheonga sp. nov. (Synechococcales, Cyanobacteria) isolated from a Daecheong Lake in Geum River, Republic of Korea

Department of Life Science, College of Natural Science, Kyonggi University, Suwon 16227, Republic of Korea
Department of Biotechnology, Sangmyung University, Seoul 03016, Republic of Korea; Present address: Geum River Environment Research Center, National Institute of Environmental Research, Chungbuk 29027, Republic of Korea
Department of Biotechnology, Sangmyung University, Seoul 03016, Republic of Korea
Department of Life Science, College of Natural Science, Kyonggi University, Suwon 16227, Republic of Korea
cryptic species Daecheong Lake phylogenetic analysis Pinocchia Algae

Abstract

The collection of Pinocchia daecheonga sp. nov. was performed at the Daecheong Lake in Korea, five strains of which with four clones were investigated through light microscopy, transmission electron microscopy and molecular data comprising the 16S rRNA to 23S rRNA gene. P. daecheonga differed from type species P. polymorpha by absence of the polar aerotopes. Investigated stains and four clones of P. daecheonga turned out to be a sister clade to the P. polymorpha according to the phylogenetic analysis of 16S rRNA gene. In addition, the Pinocchia was clustered with the family Leptolyngbyaceae members, genera Leptothoe and Leptoelongatus. 16S–23S rRNA intergenic transcribed spacer (ITS) region of P. daecheonga was found to be substantially distinct to P. polymorpha in terms of the secondary structure and nucleotide sequence composition, which concludes that the Pinocchia daecheonga isolated from the Daecheong Lake is a unique species due to differences in morphology and genetic traits compared to the relative P. polymorpha.

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